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Apbb1 (BC048395) Mouse Tagged ORF Clone
CAT＃: MR206000
- TrueORF®
Apbb1 (Myc-DDK-tagged) - Mouse amyloid beta (A4) precursor protein-binding, family B, member 1 (cDNA clone MGC:56969 IMAGE:6392016)
ORF Plasmid: tGFP
"BC048395" in other vectors (4)
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CNY 3,656.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Fe65; Rir |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR206000 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTTGGGACTGAAGGACCCCGAGGAGGCGACATTGTCCTTCCCAGCTCAGAGCCTCAGCCCAGAAC CAGTTCCCCAGGAGGAAGAGAAGCTGTCCCAACGGAATGCCAACCCAGGGATCAAGTGTTTCGCTGTGCG CTCCCTAGGCTGGGTAGAGATGACCGAGGAGGAGCTGGCCCCAGGACGCAGCAGTGTGGCAGTCAACAAT TGTATCCGCCAGCTCTCCTACCACAAAAACAATCTACATGATCCGATGGCTGGGGGCTGGGGAGAGGGAA AGGATCTGCTGCTCCAGCTGGAGGACGAGACTCTAAAGTTGGTGGAGCCACAGAACCAGACGCTGCTGCA TGCACAGCCCATCGTCAGCATTCGTGTGTGGGGCGTTGGGCGGGACAGTGGAAGAGAGAGGGACTTTGCC TACGTAGCTCGAGATAAGCTGACCCAGATGCTCAAGTGCCACGTGTTTCGCTGTGAGGCACCTGCCAAGA ACATCGCCACCAGCCTGCATGAGATCTGCTCCAAGATCATGTCTGAACGGCGCAATGCTCGCTGCTTGGT CAATGGACTCTCCCTAGACCACTCTAAACTCGTGGATGTCCCTTTCCAAGTGGAATTCCCAGCACCAAAG AATGAGCTGGTGCAGAAGTTCCAAGTCTATTACCTGGGAAATGTGCCAGTTGCTAAACCTGTTGGGGTAG ACGTGATTAATGGGGCCCTGGAGTCAGTCCTGTCTTCCAGTAGCCGTGAGCAGTGGACTCCAAGTCACGT CAGCGTGGCCCCTGCCACCCTCACCATCTTGCACCAGCAGACAGAAGCGGTGCTGGGGGAGTGCCGGGTG CGGTTTCTCTCCTTCCTGGCTGTGGGCAGAGATGTGCACACATTCGCGTTCATCATGGCTGCCGGCCCAG CCTCCTTCTGCTGTCACATGTTTTGGTGTGAGCCCAATGCTGCCAGTCTCTCAGAGGCTGTGCAGGCTGC ATGCATGCTCCGCTACCAGAAGTGTCTGGATGCTCGCTCCCAGACCTCCACCTCCTGCCTCCCAGCACCC CCTGCGGAGTCAGTTGCAAGACGTGTAGGGTGGACAGTCCGCAGGGGTGTTCAGTCGCTGTGGGGTTCCC TCAAGCCCAAACGTCTGGGATCCCAGACCCCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR206000 protein sequence
Red=Cloning site Green=Tags(s) MELGLKDPEEATLSFPAQSLSPEPVPQEEEKLSQRNANPGIKCFAVRSLGWVEMTEEELAPGRSSVAVNN CIRQLSYHKNNLHDPMAGGWGEGKDLLLQLEDETLKLVEPQNQTLLHAQPIVSIRVWGVGRDSGRERDFA YVARDKLTQMLKCHVFRCEAPAKNIATSLHEICSKIMSERRNARCLVNGLSLDHSKLVDVPFQVEFPAPK NELVQKFQVYYLGNVPVAKPVGVDVINGALESVLSSSSREQWTPSHVSVAPATLTILHQQTEAVLGECRV RFLSFLAVGRDVHTFAFIMAAGPASFCCHMFWCEPNAASLSEAVQAACMLRYQKCLDARSQTSTSCLPAP PAESVARRVGWTVRRGVQSLWGSLKPKRLGSQTP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC048395 |
| ORF Size | 1152 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC048395, AAH48395 |
| RefSeq Size | 2065 bp |
| RefSeq ORF | 1154 bp |
| Locus ID | 11785 |
| MW | 42.4 kDa |
| Gene Summary | Adapter protein that forms a transcriptionally active complex with the gamma-secretase-derived amyloid precursor protein (APP) intracellular domain. Plays a central role in the response to DNA damage by translocating to the nucleus and inducing apoptosis. May act by specifically recognizing and binding histone H2AX phosphorylated on 'Tyr-142' (H2AXY142ph) at double-strand breaks (DSBs), recruiting other pro-apoptosis factors such as MAPK8/JNK1. Required for histone H4 acetylation at double-strand breaks (DSBs). Its ability to specifically bind modified histones and chromatin modifying enzymes such as KAT5/TIP60, probably explains its transcription activation activity. Function in association with TSHZ3, SET and HDAC factors as a transcriptional repressor, that inhibits the expression of CASP4. Associates with chromatin in a region surrounding the CASP4 transcriptional start site(s).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217686 | Apbb1 (untagged) - Mouse amyloid beta (A4) precursor protein-binding, family B, member 1 (cDNA clone MGC:56969 IMAGE:6392016), (10ug) |
CNY 6,940.00 |
|
| MG206000 | Apbb1 (tGFP-tagged) - Mouse amyloid beta (A4) precursor protein-binding, family B, member 1 (cDNA clone MGC:56969 IMAGE:6392016) |
CNY 2,380.00 |
|
| MR206000L3 | Lenti ORF clone of Apbb1 (Myc-DDK-tagged) - Mouse amyloid beta (A4) precursor protein-binding, family B, member 1 (cDNA clone MGC:56969 IMAGE:6392016) |
CNY 4,090.00 |
|
| MR206000L4 | Lenti ORF clone of Apbb1 (mGFP-tagged) - Mouse amyloid beta (A4) precursor protein-binding, family B, member 1 (cDNA clone MGC:56969 IMAGE:6392016) |
CNY 4,090.00 |
