Apbb1 (BC048395) Mouse Tagged ORF Clone

CAT#: MG206000

  • TrueORF®

Apbb1 (tGFP-tagged) - Mouse amyloid beta (A4) precursor protein-binding, family B, member 1 (cDNA clone MGC:56969 IMAGE:6392016)

ORF Plasmid: DDK tGFP



  "BC048395" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 2,380.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


Rabbit polyclonal Anti-Apbb1 Antibody
    • 50 ug

CNY 4,628.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms Fe65; Rir
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG206000 representing BC048395
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGTTGGGACTGAAGGACCCCGAGGAGGCGACATTGTCCTTCCCAGCTCAGAGCCTCAGCCCAGAAC
CAGTTCCCCAGGAGGAAGAGAAGCTGTCCCAACGGAATGCCAACCCAGGGATCAAGTGTTTCGCTGTGCG
CTCCCTAGGCTGGGTAGAGATGACCGAGGAGGAGCTGGCCCCAGGACGCAGCAGTGTGGCAGTCAACAAT
TGTATCCGCCAGCTCTCCTACCACAAAAACAATCTACATGATCCGATGGCTGGGGGCTGGGGAGAGGGAA
AGGATCTGCTGCTCCAGCTGGAGGACGAGACTCTAAAGTTGGTGGAGCCACAGAACCAGACGCTGCTGCA
TGCACAGCCCATCGTCAGCATTCGTGTGTGGGGCGTTGGGCGGGACAGTGGAAGAGAGAGGGACTTTGCC
TACGTAGCTCGAGATAAGCTGACCCAGATGCTCAAGTGCCACGTGTTTCGCTGTGAGGCACCTGCCAAGA
ACATCGCCACCAGCCTGCATGAGATCTGCTCCAAGATCATGTCTGAACGGCGCAATGCTCGCTGCTTGGT
CAATGGACTCTCCCTAGACCACTCTAAACTCGTGGATGTCCCTTTCCAAGTGGAATTCCCAGCACCAAAG
AATGAGCTGGTGCAGAAGTTCCAAGTCTATTACCTGGGAAATGTGCCAGTTGCTAAACCTGTTGGGGTAG
ACGTGATTAATGGGGCCCTGGAGTCAGTCCTGTCTTCCAGTAGCCGTGAGCAGTGGACTCCAAGTCACGT
CAGCGTGGCCCCTGCCACCCTCACCATCTTGCACCAGCAGACAGAAGCGGTGCTGGGGGAGTGCCGGGTG
CGGTTTCTCTCCTTCCTGGCTGTGGGCAGAGATGTGCACACATTCGCGTTCATCATGGCTGCCGGCCCAG
CCTCCTTCTGCTGTCACATGTTTTGGTGTGAGCCCAATGCTGCCAGTCTCTCAGAGGCTGTGCAGGCTGC
ATGCATGCTCCGCTACCAGAAGTGTCTGGATGCTCGCTCCCAGACCTCCACCTCCTGCCTCCCAGCACCC
CCTGCGGAGTCAGTTGCAAGACGTGTAGGGTGGACAGTCCGCAGGGGTGTTCAGTCGCTGTGGGGTTCCC
TCAAGCCCAAACGTCTGGGATCCCAGACCCCA


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG206000 representing BC048395
Red=Cloning site Green=Tags(s)

MELGLKDPEEATLSFPAQSLSPEPVPQEEEKLSQRNANPGIKCFAVRSLGWVEMTEEELAPGRSSVAVNN
CIRQLSYHKNNLHDPMAGGWGEGKDLLLQLEDETLKLVEPQNQTLLHAQPIVSIRVWGVGRDSGRERDFA
YVARDKLTQMLKCHVFRCEAPAKNIATSLHEICSKIMSERRNARCLVNGLSLDHSKLVDVPFQVEFPAPK
NELVQKFQVYYLGNVPVAKPVGVDVINGALESVLSSSSREQWTPSHVSVAPATLTILHQQTEAVLGECRV
RFLSFLAVGRDVHTFAFIMAAGPASFCCHMFWCEPNAASLSEAVQAACMLRYQKCLDARSQTSTSCLPAP
PAESVARRVGWTVRRGVQSLWGSLKPKRLGSQTP

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC048395
ORF Size 1154 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC048395, AAH48395
RefSeq Size 2065 bp
RefSeq ORF 1154 bp
Locus ID 11785
Gene Summary Adapter protein that forms a transcriptionally active complex with the gamma-secretase-derived amyloid precursor protein (APP) intracellular domain. Plays a central role in the response to DNA damage by translocating to the nucleus and inducing apoptosis. May act by specifically recognizing and binding histone H2AX phosphorylated on 'Tyr-142' (H2AXY142ph) at double-strand breaks (DSBs), recruiting other pro-apoptosis factors such as MAPK8/JNK1. Required for histone H4 acetylation at double-strand breaks (DSBs). Its ability to specifically bind modified histones and chromatin modifying enzymes such as KAT5/TIP60, probably explains its transcription activation activity. Function in association with TSHZ3, SET and HDAC factors as a transcriptional repressor, that inhibits the expression of CASP4. Associates with chromatin in a region surrounding the CASP4 transcriptional start site(s).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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