Eif4b (BC007171) Mouse Tagged ORF Clone

CAT#: MR205574

  • TrueORF®

Eif4b (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 4B (cDNA clone MGC:7530 IMAGE:3492114)

ORF Plasmid: DDK tGFP



  "BC007171" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 3,656.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms 2310046H11Rik; AL024095; C85189; Eif4a2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR205574 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACCGCTATGGGGGCCGGGATCGCTATGATGACCGAGGCAGCAGAGACTATGACCGAGGCTATGACT
CCAGGATAGGCAGTGGCAGAAGAGCATTTGGAAGTGGGTACCGGAGAGATGATGACTACAGAGGAGGTGG
GGACCGCTATGAAGACCGCTATGACAGACGGGATGATCGGTCGTGGAGCTCCAGGGATGACTACTCTCGG
GATGATTATAGGCGTGATGACAGAGGTCCCCCCCAGAGACCCAGACTGAACCTCAAGCCTCGCAGCGCTC
CTAAGGAGGATGACGCCTCCGCCAGCACCTCCCAGTCCAGCCGGGCAGCCTCCATCTTTGGAGGGGCGAA
GCCTGTTGACACAGCTGCTAGGGAAAGAGAAGTAGAGGAGCGGCTACAGAAGGAGCAGGAGAAGCTGCAG
CGTCAGCTGGATGAGCCAAAACTAGACCGCCGGCCCCGGGAGAGACACCCAAGCTGGCGAAGTGAAGAAA
CTCAGGAAAGAGAACGGTCAAGGACAGGAAGTGAGTCATCGCAGACTGGGGCCTCAGCCACATCTGGCAG
AAATACACGAAGGAGAGAGAGTGAGAAGTCTCTAGAAAATGAAACCCTCAATAAAGAAGAAGACTGTCAC
TCTCCAACCTCTAAGCCTCCTAAACCTGACCAGCCTCTAAAGGTAATGCCAGCCCCTCCACCAAAGGAGA
ATGCGTGGGTGAAGCGAAGCTCTAACCCTCCTGCCCGATCTCAGAGCTCAGACACAGAGCAGCCGTCCCC
TACAAGTGGTGGAGGGAAAGTAGCTGCAGTCCAGCCCCCTGAGGAAGGACCATCAAGAAAAGATGGAAAT
AAAGTGGATGTGGTGGGTGCCACACAAGGCCAAGCTGGAAGCTGCAGCCGTGGTCCCGGGGATGGAGGGA
GCAGAGACCACTGGAAGGACTTGGATAGGAAGGATGGCAAAAAAGATCAAGATTCCAGATCTGCACCTGA
GCCAAAGAAACCTGAGGAGAACCCAGCCTCCAAGTTCAGCTCTGCAAGCAAGTACGCTGCTCTGTCTGTG
GATGGCGAGGATGAGGATGAGGGCGACGACTGCACTGAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR205574 protein sequence
Red=Cloning site Green=Tags(s)

MDRYGGRDRYDDRGSRDYDRGYDSRIGSGRRAFGSGYRRDDDYRGGGDRYEDRYDRRDDRSWSSRDDYSR
DDYRRDDRGPPQRPRLNLKPRSAPKEDDASASTSQSSRAASIFGGAKPVDTAAREREVEERLQKEQEKLQ
RQLDEPKLDRRPRERHPSWRSEETQERERSRTGSESSQTGASATSGRNTRRRESEKSLENETLNKEEDCH
SPTSKPPKPDQPLKVMPAPPPKENAWVKRSSNPPARSQSSDTEQPSPTSGGGKVAAVQPPEEGPSRKDGN
KVDVVGATQGQAGSCSRGPGDGGSRDHWKDLDRKDGKKDQDSRSAPEPKKPEENPASKFSSASKYAALSV
DGEDEDEGDDCTE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC007171
ORF Size 1089 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC007171, AAH07171
RefSeq Size 3658 bp
RefSeq ORF 1091 bp
Locus ID 75705
MW 40.7 kDa
Gene Summary Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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