Eif4b (BC007171) Mouse Untagged Clone
CAT#: MC206803
Eif4b (untagged) - Mouse eukaryotic translation initiation factor 4B (cDNA clone MGC:7530 IMAGE:3492114), (10ug)
CNY 3,656.00
Product images
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | 2310046H11Rik; AL024095; C85189; Eif4a2 |
Vector | PCMV6-Kan/Neo |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>BC007171
GAATAAGAAGGGGAAGACCATCTCCCTAACAGACTTTCTAGCTGAGGATGGAGGACCTGGTGGAGGAAGC ACCTATGTCCCCAAACCAGTCAGCTGGGCTGATGAAACAGACGATCTGGAAGGAGATGTGTCAACAACTT GGCATAGTAACGATGATGACGTGTACAGGGCGCCTCCAATTGACCGTTCCATCCTTCCCACTGCTCCACG GGCTGCTCGGGAACCCAATATTGACCGGAGCCGTCTTCCCAAGTCGCCACCCTACACTGCTTTCCTAGGG AATCTGCCCTATGATGTGACAGAAGACTCCATTAAGGATTTCTTTAGAGGATTAAATATCAGCGCTGTAC GCTTACCACGGGAACCCAGCAATCCAGACAGGTTGAAAGGTTTCGGCTACGCAGAATTTGAGGACCTGGA TTCTCTGCTCAGTGCTCTGAGTCTCAATGAAGAGTCTCTAGGTAACAGGAGAATTCGTGTGGATGTTGCT GATCAAGCACAGGATAAAGACAGGGATGACCGTTCTTTTGGTCGAGATAGAAATCGGGATTCTGACAAAA CAGACACAGACTGGAGGGCCCGTCCCACCACAGACAGTTTTGATGACTACCCACCTAGAAGAGGCGATGA TAGCTTTGGAGACAAGTATCGAGATCGTTACGATTCAGACCGGTATCGGGATGGGTATAGGGACGGATAT CGGGACGGCCCACGCAGAGACATGGACCGCTATGGGGGCCGGGATCGCTATGATGACCGAGGCAGCAGAG ACTATGACCGAGGCTATGACTCCAGGATAGGCAGTGGCAGAAGAGCATTTGGAAGTGGGTACCGGAGAGA TGATGACTACAGAGGAGGTGGGGACCGCTATGAAGACCGCTATGACAGACGGGATGATCGGTCGTGGAGC TCCAGGGATGACTACTCTCGGGATGATTATAGGCGTGATGACAGAGGTCCCCCCCAGAGACCCAGACTGA ACCTCAAGCCTCGCAGCGCTCCTAAGGAGGATGACGCCTCCGCCAGCACCTCCCAGTCCAGCCGGGCAGC CTCCATCTTTGGAGGGGCGAAGCCTGTTGACACAGCTGCTAGGGAAAGAGAAGTAGAGGAGCGGCTACAG AAGGAGCAGGAGAAGCTGCAGCGTCAGCTGGATGAGCCAAAACTAGACCGCCGGCCCCGGGAGAGACACC CAAGCTGGCGAAGTGAAGAAACTCAGGAAAGAGAACGGTCAAGGACAGGAAGTGAGTCATCGCAGACTGG GGCCTCAGCCACATCTGGCAGAAATACACGAAGGAGAGAGAGTGAGAAGTCTCTAGAAAATGAAACCCTC AATAAAGAAGAAGACTGTCACTCTCCAACCTCTAAGCCTCCTAAACCTGACCAGCCTCTAAAGGTAATGC CAGCCCCTCCACCAAAGGAGAATGCGTGGGTGAAGCGAAGCTCTAACCCTCCTGCCCGATCTCAGAGCTC AGACACAGAGCAGCCGTCCCCTACAAGTGGTGGAGGGAAAGTAGCTGCAGTCCAGCCCCCTGAGGAAGGA CCATCAAGAAAAGATGGAAATAAAGTGGATGTGGTGGGTGCCACACAAGGCCAAGCTGGAAGCTGCAGCC GTGGTCCCGGGGATGGAGGGAGCAGAGACCACTGGAAGGACTTGGATAGGAAGGATGGCAAAAAAGATCA AGATTCCAGATCTGCACCTGAGCCAAAGAAACCTGAGGAGAACCCAGCCTCCAAGTTCAGCTCTGCAAGC AAGTACGCTGCTCTGTCTGTGGATGGCGAGGATGAGGATGAGGGCGACGACTGCACTGAGTAGGACCTCC GCACCCTGTGCTTTCTCCTAGTCTCCACCCCGGAACATTCAAGAGCAAATCAAACCTCTATCCAGACAAG ACAAAATAAACTCACCATCTCCTGAAGACCTTTCTAAACTTTTTTTTTTAATGAAATGAAATTCTTTTGC ATGCTGCTGCAGCCTTTTAAAGTATTGAAGTAACTGGAGAATCACCATTGTAGCCAGAGACGTGGCTACC GCTTTTGAATGGGGAAGTTGTGTCTGACAATACCAAGCAGTTACTGTGTGACTAGTGCCTGGGGCTCCAT TCAGCAGAATTGCAGTGACAGAGAGGCAGTCACATCAGTTAGGCAGTAGAAAAAGGTGCAAAGCAGAAGT GAGATTTCTACCTTCTAATTCTTATCCTGAGATGGCACCTGTGAATACCCAAACATTACCTGAATAAATT TCCCATCCTTAGAAAGGTAGCTCTAGTCTCAAGCTGTTTTAGTCTCCAGGAGGCTGCCAGCCCCTCTACT TTAGTTCTGACTTCAATGGGGCCAGCCTAAGGGGGAATTGTTTTATTTTTTTACCCCCCAAGGGGGTAGT TGGGAGTGAGTCTACGGGGCACTAACAAGTAGTTCCGCTTGGGGACCAAGATGAACGGAAACTCAATCGT GGTTTGGAAAGCTTTGGGGAGGTGACTAATTATATTTTATACCCGGTAGTGAGGGAAACAGTGTGACCTC CAGTAAACACATACATAGAATGGTCATTTCTGGATGAGGAAGCACTTAACAGGTAGTGAGAAGGCCTGGC TTTGCCCCTTCTAAACTCTCCTTTCTAGAGGTTGAAATATACCCATATTTTTTTGAAAACCTTGTCACTC TGGTCATCTCATGGTCTCCATGACACTTAGCCTCCCAGAGCTGTCTGTGAGTCACACACCTCCCACAGAC ACTGTATCTTAGCCACAAAAAGCCATGGCCTGAGGACTCGCTGCTTTTCTCAAGCAAGCCAGTGAGTTTT CTCTGTGCCATCTTTGATTTCTGCCTGCTCCTGCAGCTTTCAGCCTCAACTGATGGGCTTGGGTGGTGAA TAGGGGTGTGTAAATGGGGTGAGTTGGAGGTCTTCAGCCTTGAATGGGGTGTAAGGTTTCTATAAATCTC CCTCACTGGAGCCCCACGCTGGTGCTTGTTTATAATGTATGTGACCCTGACTGGTTTTCTGCTTAGGAGG GTTGGCTTCTTTACTCCCCTCAAGAATAAAGGATAATGCAAAGAACTATTGAGCAAGGTCCTGGTTTCTC TTGCAACACTGTAGCTTGACCTGTCTGCTTTGATGTGCACCTTTGTAGGGGTCAGCTCCAAAGATGGCGA GCCTCCCTGTTGTGGGTAGTCAGGCACCTGCAGACAGACGATACCTTGGTCCTAGGCCTGCTGTGCAGGA GCATTGATGTCCCGTGCTGCAGCAGCAGGCACAGCAAATCGCTTCTCCGGTGGATTTGATTGGCTAGTAG CTAGGAAGTGGGCTTTTGCAGATACCCACAGTTGAATGGGTTTTTTTAAACCATTGTTGTAGACAATGAG AAGGTAGTTTGCCCTCTTCATGTCTATTTCCCTTCCAGATAGTTGCATCCGAAGACAGCTGTTTTGTCCC ACTCTCCTACTTTGCTCCTCTGTTAAAATAAAAACAGGGGTTGACTTATGTCCCGCTCCTGAATACATGT AAAATTTGTACAAAAATATCTTCTATGAAAATGATTTGTAATCTGTAGACATTACTTGGGAGATGTCTTG AGATGTAAAATCCCATCCTTTGGGTTGAGGGTTTTTTTGTTTTTCTCCAAATAAATCCAGTCTTTAAAGT TGAAAAAAAAAAAAAAAA |
Restriction Sites | RsrII-NotI |
ACCN | BC007171 |
Insert Size | 1092 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | BC007171, AAH07171 |
RefSeq Size | 3658 bp |
RefSeq ORF | 1092 bp |
Locus ID | 75705 |
Gene Summary | Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F (By similarity).[UniProtKB/Swiss-Prot Function] |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG205574 | Eif4b (tGFP-tagged) - Mouse eukaryotic translation initiation factor 4B (cDNA clone MGC:7530 IMAGE:3492114) |
CNY 2,280.00 |
|
MR205574 | Eif4b (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 4B (cDNA clone MGC:7530 IMAGE:3492114) |
CNY 3,656.00 |
|
MR205574L3 | Lenti ORF clone of Eif4b (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 4B (cDNA clone MGC:7530 IMAGE:3492114) |
CNY 3,990.00 |
|
MR205574L4 | Lenti ORF clone of Eif4b (mGFP-tagged) - Mouse eukaryotic translation initiation factor 4B (cDNA clone MGC:7530 IMAGE:3492114) |
CNY 3,990.00 |