Rsad2 (NM_021384) Mouse Tagged ORF Clone
CAT#: MR205564
- TrueORF®
Rsad2 (Myc-DDK-tagged) - Mouse radical S-adenosyl methionine domain containing 2 (Rsad2)
ORF Plasmid: tGFP
"NM_021384" in other vectors (4)
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CNY 3,656.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 2510004L01Rik; cig5; Vig1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR205564 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGATGCTGGTGCCCACTGCTCTAGCTGCTCGGCTGCTGAGCCTGTTCCAGCAGCAGCTGGGTTCCC TCTGGAGTGGCCTGGCCATCCTGTTCTGCTGGCTGAGAATAGCATTAGGGTGGCTAGATCCCGGGAAGGA ACAGCCACAGGTCCGGGGTGAGCTGGAGGAGACCCAGGAGACCCAGGAAGATGGGAACAGCACTCAGCGC ACAACCCCCGTGAGTGTCAACTACCACTTCACTCGTCAGTGCAACTACAAATGTGGCTTCTGCTTCCACA CAGCCAAGACATCCTTCGTGCTGCCCCTGGAGGAGGCCAAGCGAGGACTGCTTCTGCTCAAACAGGCTGG TTTGGAGAAGATCAACTTTTCTGGAGGAGAACCCTTCCTTCAGGACAGGGGTGAATACTTGGGCAAGCTT GTGAGATTCTGCAAGGAGGAGCTAGCCCTGCCCTCTGTGAGCATAGTGAGCAATGGCAGCCTTATCCGGG AGAGATGGTTCAAGGACTATGGGGAGTATTTGGACATTCTTGCTATCTCCTGCGACAGCTTCGATGAGCA GGTTAATGCTCTGATTGGCCGTGGTCAAGGAAAAAAGAACCACGTGGAAAACCTTCAAAAGCTGAGGAGG TGGTGCAGGGATTACAAGGTGGCTTTCAAGATCAACTCTGTCATTAATCGCTTCAACGTGGACGAAGACA TGAATGAACACATCAAGGCCCTGAGCCCTGTGCGCTGGAAGGTTTTCCAGTGCCTCCTAATTGAGGGTGA GAACTCAGGAGAAGATGCCCTGAGGGAAGCAGAAAGATTTCTTATAAGCAATGAAGAATTTGAAACATTC TTGGAGCGTCACAAAGAGGTGTCCTGTTTGGTGCCTGAATCTAACCAGAAGATGAAAGACTCCTACCTTA TCCTAGATGAATATATGCGCTTTCTGAACTGTACCGGTGGCCGGAAGGACCCTTCCAAGTCTATTCTGGA TGTTGGCGTGGAAGAAGCAATAAAGTTCAGTGGATTTGATGAGAAGATGTTTCTGAAGCGTGGCGGAAAG TATGTGTGGAGTAAAGCTGACCTGAAGCTGGACTGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR205564 protein sequence
Red=Cloning site Green=Tags(s) MGMLVPTALAARLLSLFQQQLGSLWSGLAILFCWLRIALGWLDPGKEQPQVRGELEETQETQEDGNSTQR TTPVSVNYHFTRQCNYKCGFCFHTAKTSFVLPLEEAKRGLLLLKQAGLEKINFSGGEPFLQDRGEYLGKL VRFCKEELALPSVSIVSNGSLIRERWFKDYGEYLDILAISCDSFDEQVNALIGRGQGKKNHVENLQKLRR WCRDYKVAFKINSVINRFNVDEDMNEHIKALSPVRWKVFQCLLIEGENSGEDALREAERFLISNEEFETF LERHKEVSCLVPESNQKMKDSYLILDEYMRFLNCTGGRKDPSKSILDVGVEEAIKFSGFDEKMFLKRGGK YVWSKADLKLDW TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_021384 |
ORF Size | 1089 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_021384.1, NM_021384.2, NM_021384.3, NM_021384.4, NP_067359.2 |
RefSeq Size | 3785 bp |
RefSeq ORF | 1089 bp |
Locus ID | 58185 |
UniProt ID | Q8CBB9 |
MW | 41.6 kDa |
Gene Summary | Interferon-inducible iron-sulfur (4FE-4S) cluster-binding antiviral protein which plays a major role in the cell antiviral state induced by type I and type II interferon. Can inhibit a wide range of viruses, including west Nile virus (WNV), dengue virus, sindbis virus, influenza A virus, sendai virus and vesicular stomatitis virus (VSV). Displays antiviral activity against influenza A virus by inhibiting the budding of the virus from the plasma membrane by disturbing the lipid rafts. This is accomplished, at least in part, through binding and inhibition of the enzyme farnesyl diphosphate synthase (FPPS), which is essential for the biosynthesis of isoprenoid-derived lipids. Promotes TLR7 and TLR9-dependent production of IFN-beta production in plasmacytoid dendritic cells (pDCs) by facilitating Lys-63'-linked ubiquitination of IRAK1. Plays a role in CD4+ T-cells activation and differentiation. Facilitates T-cell receptor (TCR)-mediated GATA3 activation and optimal T-helper 2 (Th2) cytokine production by modulating NFKB1 and JUNB activities. Can inhibit secretion of soluble proteins.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC203237 | Rsad2 (untagged) - Mouse radical S-adenosyl methionine domain containing 2 (Rsad2), (10ug) |
CNY 3,656.00 |
|
MG205564 | Rsad2 (tGFP-tagged) - Mouse radical S-adenosyl methionine domain containing 2 (Rsad2) |
CNY 3,140.00 |
|
MR205564L3 | Lenti ORF clone of Rsad2 (Myc-DDK-tagged) - Mouse radical S-adenosyl methionine domain containing 2 (Rsad2) |
CNY 4,750.00 |
|
MR205564L4 | Lenti ORF clone of Rsad2 (mGFP-tagged) - Mouse radical S-adenosyl methionine domain containing 2 (Rsad2) |
CNY 4,750.00 |