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Rnls (NM_001167818) Mouse Tagged ORF Clone
CAT＃: MR204181
- TrueORF®
Rnls (Myc-DDK-tagged) - Mouse renalase, FAD-dependent amine oxidase (Rnls), transcript variant 1
"NM_001167818" in other vectors (3)
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CNY 2,400.00
CNY 2,461.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 6530404N21Rik; AI452315; AW060440; C10orf59 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR204181 representing NM_001167818
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATTACTGCCAGCAGTCCTCATAATCCCCGATGCACAGCTGACTTGGGAGCTCAGTACATCACCTGCT CTCCTCATTATGTCAAAGAGCACCAAAATTTTTATGAGGAACTGTTAGCTCATGGAATTTTGAAGCCTCT GACATCCCCCATTGAAGGAATGAAAGGGAAGGAAGGAGATTGCAACTTTGTGGCACCTCAAGGATTTTCT TCAGTTATCAAGTACTACTTGAAAAAGTCAGGTGCAGAAGTCTCCCTCAAGCACTGTGTGACTCAGATCC ACCTGAAAGATAACAAGTGGGAAGTCTCCACAGACACTGGCTCTGCTGAGCAGTTTGACCTTGTCATCCT CACCATGCCAGCTCCTCAGATTCTGGAACTTCAAGGTGACATTGTGAACTTAATTAGTGAACGCCAGAGG GAGCAACTGAAATCTGTGAGCTACTCCTCTCGCTATGCTCTGGGCCTCTTTTATGAAGTAGGCATGAAGA TTGGTGTCCCTTGGTCCTGCCGCTACCTCAGCAGTCACCCCTGCATATGCTTCATCTCCATTGATAATAA GAAGCGCAATATAGAGTCATCAGAATGTGGTCCATCCGTGGTGATCCAAACCACTGTCCCATTTGGAGTT CAACACTTGGAGGCCAGTGAGGCGGATGTGCAGAAGTTAATGATCCAGCAATTGGAAACCATTCTGCCGG GTTTGCCTCAGCCAGTTGCTACCATATGCCATAAATGGACATATTCACAGGTTACAAGCTCAGTTTCCGA CAGACCTGGTCAGATGACTCTTCATCTCAAGCCTTTCCTGGTGTGCGGAGGGGATGGATTTACTCACTCC AACTTCAATGGCTGCATCTCCTCTGCCCTGAGTGTCATGAAAGTTTTAAAGCGTTATATT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR204181 representing NM_001167818
Red=Cloning site Green=Tags(s) MITASSPHNPRCTADLGAQYITCSPHYVKEHQNFYEELLAHGILKPLTSPIEGMKGKEGDCNFVAPQGFS SVIKYYLKKSGAEVSLKHCVTQIHLKDNKWEVSTDTGSAEQFDLVILTMPAPQILELQGDIVNLISERQR EQLKSVSYSSRYALGLFYEVGMKIGVPWSCRYLSSHPCICFISIDNKKRNIESSECGPSVVIQTTVPFGV QHLEASEADVQKLMIQQLETILPGLPQPVATICHKWTYSQVTSSVSDRPGQMTLHLKPFLVCGGDGFTHS NFNGCISSALSVMKVLKRYI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001167818 |
| ORF Size | 900 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001167818.1, NP_001161290.1 |
| RefSeq Size | 1632 bp |
| RefSeq ORF | 903 bp |
| Locus ID | 67795 |
| UniProt ID | A7RDN6 |
| MW | 33.3 kDa |
| Gene Summary | Catalyzes the oxidation of the less abundant 1,2-dihydro-beta-NAD(P) and 1,6-dihydro-beta-NAD(P) to form beta-NAD(P)(+). The enzyme hormone is secreted by the kidney, and circulates in blood and modulates cardiac function and systemic blood pressure. Lowers blood pressure in vivo by decreasing cardiac contractility and heart rate and preventing a compensatory increase in peripheral vascular tone, suggesting a causal link to the increased plasma catecholamine and heightened cardiovascular risk. High concentrations of catecholamines activate plasma renalase and promotes its secretion and synthesis.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218391 | Rnls (untagged) - Mouse renalase, FAD-dependent amine oxidase (Rnls), transcript variant 1, (10ug) |
CNY 2,400.00 |
|
| MR204181L3 | Lenti ORF clone of Rnls (Myc-DDK-tagged) - Mouse renalase, FAD-dependent amine oxidase (Rnls), transcript variant 1 |
CNY 4,750.00 |
|
| MR204181L4 | Lenti ORF clone of Rnls (mGFP-tagged) - Mouse renalase, FAD-dependent amine oxidase (Rnls), transcript variant 1 |
CNY 4,750.00 |
