Ifih1 (NM_027835) Mouse Tagged ORF Clone
CAT#: MG226446
- TrueORF®
Ifih1 (tGFP-tagged) - Mouse interferon induced with helicase C domain 1 (Ifih1) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_027835" in other vectors (4)
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CNY 11,310.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 9130009C22Rik; Helicard; Hlcd; MDA5; RLR-2 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG226446 representing NM_027835
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGATTGTCTGTTCTGCAGAGGACAGCTTCAGGAATCTCATCTTATTCTTCAGGCCCAGGCTGAAAA TGTACATTCAGGTGGAGCCAGTGCTGGACCACCTCATCTTTCTGTCTGCAGAAACCAAAGAGCAGATTCT TAAAAAGATCAACACCTGTGGTAACACCAGCGCGGCAGAACTGCTGCTGAGCACCTTGGAGCAGGGACAA TGGCCTCTGGGATGGACGCAGATGTTCGTGGAGGCCCTAGAGCACAGTGGCAATCCCCTAGCCGCGCGCT ATGTCAAACCCACACTCACTGATCTGCCCTCTCCTTCCTCTGAGACTGCCCATGACGAGTGTCTCCACTT GCTGACCCTCCTCCAGCCCACTTTGGTGGACAAACTTCTGATTAACGATGTCTTGGACACTTGCTTCGAG AAGGGACTATTGACAGTCGAAGACAGAAATCGGATTTCTGCTGCAGGAAACAGCGGGAATGAGTCAGGTG TAAGAGAGCTGCTGAGAAGGATTGTGCAGAAGGAAAACTGGTTTTCTACCTTCCTGGATGTTCTGCGCCA AACTGGAAATGATGCACTATTCCAAGAACTAACAGGTGGAGGCTGCCCAGAAGACAACACAGACTTGGCT AACTCGTCTCACAGAGATGGGCCTGCAGCTAATGAGTGTCTTCTGCCTGCTGTCGATGAGTCAAGTCTGG AGACAGAGGCCTGGAACGTAGACGACATATTACCAGAGGCTTCTTGTACAGATTCGTCTGTGACCACAGA ATCAGACACAAGTTTGGCAGAAGGAAGTGTCAGCTGCTTCGATGAAAGTCTTGGACATAACAGCAACATG GGCAGGGATTCAGGCACCATGGGAAGTGATTCAGATGAAAGTGTCATCCAGACAAAAAGAGTATCCCCCG AGCCAGAACTGCAGCTCAGGCCTTACCAAATGGAAGTGGCCCAACCAGCTCTAGATGGGAAGAATATTAT TATCTGCCTCCCCACGGGGAGTGGGAAAACCAGAGTGGCTGTTTACATCACCAAAGATCACTTAGACAAG AAGAAGCAGGCATCTGAATCCGGGAAGGTTATCGTTCTTGTCAATAAGGTAATGTTAGCAGAACAACTTT TCCGAAAAGAGTTCAACCCATATTTGAAGAAATGGTATCGAATTATTGGATTAAGTGGCGATACCCAGCT GAAAATATCATTTCCAGAAGTTGTCAAATCTTACGATGTTATTATCAGCACTGCTCAAATCCTTGAAAAC TCCCTCTTAAATCTGGAGAGTGGAGACGATGACGGTGTGCAGCTGTCAGACTTCTCTCTCATTATCATTG ATGAGTGCCATCACACCAACAAGGAGGCAGTCTATAACAACATCATGAGACGATATTTGAAGCAGAAGCT GAGAAACAATGACCTCAAGAAACAAAACAAACCAGCCATTCCCCTGCCGCAGATACTAGGACTGACAGCT TCACCTGGTGTTGGAGCAGCCAAAAAGCAGTCTGAGGCTGAAAAACATATTTTAAATATATGTGCCAATC TTGATGCCTTTACCATTAAAACAGTGAAAGAGAATCTTGGTCAACTCAAACACCAAATAAAGGAACCATG CAAGAAATTTGTGATTGCTGATGACACCAGAGAAAATCCATTTAAAGAGAAACTTCTAGAAATTATGGCA AGCATTCAGACTTACTGCCAAAAAAGTCCAATGTCAGATTTTGGAACCCAACATTATGAGCAGTGGGCCA TTCAAATGGAGAAAAAAGCTGCTAAAGACGGAAATCGCAAAGATCGCGTCTGTGCAGAACATTTGAGGAA GTACAACGAAGCCCTACAAATCAACGACACGATCCGAATGATTGATGCATATAGCCACCTGGAGACATTC TACACTGATGAGAAAGAAAAGAAGTTCGCAGTCCTCAATGACAGCGACAAGAGTGATGACGAGGCCAGCA GTTGCAATGACCAACTTAAGGGCGATGTAAAGAAATCTTTGAAACTGGACGAAACGGATGAATTTCTCAT GAATTTGTTCTTTGATAACAAGAAAATGTTGAAAAAACTAGCTGAAAACCCAAAATACGAGAATGAAAAA CTCATTAAATTAAGAAACACGATACTGGAACAATTCACAAGGTCTGAGGAGTCCTCCCGAGGAATTATTT TTACAAAAACACGACAGAGCACCTACGCACTTTCCCAGTGGATCATGGAAAATGCAAAGTTTGCGGAAGT TGGAGTCAAAGCGCATCACCTGATTGGCGCGGGGCACAGCAGTGAAGTCAAGCCCATGACTCAGACTGAA CAAAAAGAAGTCATTAGTAAATTTCGCACTGGCGAAATAAATCTGCTTATCGCTACGACGGTGGCAGAGG AAGGCCTGGATATCAAAGAGTGCAATATTGTTATTCGTTATGGCCTTGTCACGAACGAGATAGCCATGGT CCAGGCCCGGGGTCGAGCCAGAGCTGATGAAAGCACGTATGTCCTGGTCACCAGCAGTGGCTCAGGAGTT ACCGAACGGGAGATTGTTAATGATTTCCGAGAGAAGATGATGTATAAAGCTATTAACCGTGTTCAAAACA TGAAACCAGAGGAGTATGCACATAAGATTTTGGAATTGCAGGTGCAAAGTATCCTGGAAAAGAAAATGAA AGTCAAAAGAAGCATTGCAAAGCAATACAACGACAATCCATCGTTAATAACACTTCTCTGCAAAAATTGT AGCATGCTGGTCTGCTCGGGAGAAAACATCCATGTCATTGAGAAGATGCATCATGTCAATATGACACCAG AATTCAAGGGACTCTACATTGTAAGAGAAAACAAAGCACTGCAAAAGAAATTTGCTGATTATCAGACCAA TGGAGAGATTATCTGCAAGTGTGGCCAGGCTTGGGGAACAATGATGGTGCACAAAGGTTTAGATTTGCCT TGTCTTAAAATAAGGAATTTTGTAGTCAATTTCAAAAATAACTCACCGAAGAAACAGTACAAGAAGTGGG TGGAATTGCCTATCAGATTTCCTGATCTTGACTACTCAGAATACTGCTTGTATAGTGATGAAGAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG226446 representing NM_027835
Red=Cloning site Green=Tags(s) MSIVCSAEDSFRNLILFFRPRLKMYIQVEPVLDHLIFLSAETKEQILKKINTCGNTSAAELLLSTLEQGQ WPLGWTQMFVEALEHSGNPLAARYVKPTLTDLPSPSSETAHDECLHLLTLLQPTLVDKLLINDVLDTCFE KGLLTVEDRNRISAAGNSGNESGVRELLRRIVQKENWFSTFLDVLRQTGNDALFQELTGGGCPEDNTDLA NSSHRDGPAANECLLPAVDESSLETEAWNVDDILPEASCTDSSVTTESDTSLAEGSVSCFDESLGHNSNM GRDSGTMGSDSDESVIQTKRVSPEPELQLRPYQMEVAQPALDGKNIIICLPTGSGKTRVAVYITKDHLDK KKQASESGKVIVLVNKVMLAEQLFRKEFNPYLKKWYRIIGLSGDTQLKISFPEVVKSYDVIISTAQILEN SLLNLESGDDDGVQLSDFSLIIIDECHHTNKEAVYNNIMRRYLKQKLRNNDLKKQNKPAIPLPQILGLTA SPGVGAAKKQSEAEKHILNICANLDAFTIKTVKENLGQLKHQIKEPCKKFVIADDTRENPFKEKLLEIMA SIQTYCQKSPMSDFGTQHYEQWAIQMEKKAAKDGNRKDRVCAEHLRKYNEALQINDTIRMIDAYSHLETF YTDEKEKKFAVLNDSDKSDDEASSCNDQLKGDVKKSLKLDETDEFLMNLFFDNKKMLKKLAENPKYENEK LIKLRNTILEQFTRSEESSRGIIFTKTRQSTYALSQWIMENAKFAEVGVKAHHLIGAGHSSEVKPMTQTE QKEVISKFRTGEINLLIATTVAEEGLDIKECNIVIRYGLVTNEIAMVQARGRARADESTYVLVTSSGSGV TEREIVNDFREKMMYKAINRVQNMKPEEYAHKILELQVQSILEKKMKVKRSIAKQYNDNPSLITLLCKNC SMLVCSGENIHVIEKMHHVNMTPEFKGLYIVRENKALQKKFADYQTNGEIICKCGQAWGTMMVHKGLDLP CLKIRNFVVNFKNNSPKKQYKKWVELPIRFPDLDYSEYCLYSDED TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_027835 |
ORF Size | 3075 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_027835.3, NP_082111.2 |
RefSeq Size | 5519 bp |
RefSeq ORF | 3078 bp |
Locus ID | 71586 |
UniProt ID | Q8R5F7 |
Gene Summary | Innate immune receptor which acts as a cytoplasmic sensor of viral nucleic acids and plays a major role in sensing viral infection and in the activation of a cascade of antiviral responses including the induction of type I interferons and proinflammatory cytokines. Its ligands include mRNA lacking 2'-O-methylation at their 5' cap and long-dsRNA (>1 kb in length). Upon ligand binding it associates with mitochondria antiviral signaling protein (MAVS/IPS1) which activates the IKK-related kinases: TBK1 and IKBKE which phosphorylate interferon regulatory factors: IRF3 and IRF7 which in turn activate transcription of antiviral immunological genes, including interferons (IFNs); IFN-alpha and IFN-beta. Responsible for detecting the Picornaviridae family members such as encephalomyocarditis virus (EMCV), mengo encephalomyocarditis virus (ENMG), and theiler's murine encephalomyelitis virus (TMEV). Can also detect other viruses such as dengue virus (DENV), west Nile virus (WNV), and reovirus. Also involved in antiviral signaling in response to viruses containing a dsDNA genome, such as vaccinia virus. Plays an important role in amplifying innate immune signaling through recognition of RNA metabolites that are produced during virus infection by ribonuclease L (RNase L). May play an important role in enhancing natural killer cell function and may be involved in growth inhibition and apoptosis in several tumor cell lines.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC223239 | Ifih1 (untagged) - Mouse interferon induced with helicase C domain 1 (Ifih1), transcript variant 1, (10ug) |
CNY 10,712.00 |
|
MR226446 | Ifih1 (Myc-DDK-tagged) - Mouse interferon induced with helicase C domain 1 (Ifih1), transcript variant 1 |
CNY 10,704.00 |
|
MR226446L3 | Lenti ORF clone of Ifih1 (Myc-DDK-tagged) - Mouse interferon induced with helicase C domain 1 (Ifih1), transcript variant 1 |
CNY 12,160.00 |
|
MR226446L4 | Lenti ORF clone of Ifih1 (mGFP-tagged) - Mouse interferon induced with helicase C domain 1 (Ifih1), transcript variant 1 |
CNY 12,160.00 |