Ifih1 (NM_027835) Mouse Untagged Clone

CAT#: MC223239

Ifih1 (untagged) - Mouse interferon induced with helicase C domain 1 (Ifih1), transcript variant 1, (10ug)



  "NM_027835" in other vectors (4)

CNY 10,712.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (2)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 9130009C22Rik; Helicard; Hlcd; MDA5; RLR-2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC223239 representing NM_027835
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCGATTGTCTGTTCTGCAGAGGACAGCTTCAGGAATCTCATCTTATTCTTCAGGCCCAGGCTGAAAA
TGTACATTCAGGTGGAGCCAGTGCTGGACCACCTCATCTTTCTGTCTGCAGAAACCAAAGAGCAGATTCT
TAAAAAGATCAACACCTGTGGTAACACCAGCGCGGCAGAACTGCTGCTGAGCACCTTGGAGCAGGGACAA
TGGCCTCTGGGATGGACGCAGATGTTCGTGGAGGCCCTAGAGCACAGTGGCAATCCCCTAGCCGCGCGCT
ATGTCAAACCCACACTCACTGATCTGCCCTCTCCTTCCTCTGAGACTGCCCATGACGAGTGTCTCCACTT
GCTGACCCTCCTCCAGCCCACTTTGGTGGACAAACTTCTGATTAACGATGTCTTGGACACTTGCTTCGAG
AAGGGACTATTGACAGTCGAAGACAGAAATCGGATTTCTGCTGCAGGAAACAGCGGGAATGAGTCAGGTG
TAAGAGAGCTGCTGAGAAGGATTGTGCAGAAGGAAAACTGGTTTTCTACCTTCCTGGATGTTCTGCGCCA
AACTGGAAATGATGCACTATTCCAAGAACTAACAGGTGGAGGCTGCCCAGAAGACAACACAGACTTGGCT
AACTCGTCTCACAGAGATGGGCCTGCAGCTAATGAGTGTCTTCTGCCTGCTGTCGATGAGTCAAGTCTGG
AGACAGAGGCCTGGAACGTAGACGACATATTACCAGAGGCTTCTTGTACAGATTCGTCTGTGACCACAGA
ATCAGACACAAGTTTGGCAGAAGGAAGTGTCAGCTGCTTCGATGAAAGTCTTGGACATAACAGCAACATG
GGCAGGGATTCAGGCACCATGGGAAGTGATTCAGATGAAAGTGTCATCCAGACAAAAAGAGTATCCCCCG
AGCCAGAACTGCAGCTCAGGCCTTACCAAATGGAAGTGGCCCAACCAGCTCTAGATGGGAAGAATATTAT
TATCTGCCTCCCCACGGGGAGTGGGAAAACCAGAGTGGCTGTTTACATCACCAAAGATCACTTAGACAAG
AAGAAGCAGGCATCTGAATCCGGGAAGGTTATCGTTCTTGTCAATAAGGTAATGTTAGCAGAACAACTTT
TCCGAAAAGAGTTCAACCCATATTTGAAGAAATGGTATCGAATTATTGGATTAAGTGGCGATACCCAGCT
GAAAATATCATTTCCAGAAGTTGTCAAATCTTACGATGTTATTATCAGCACTGCTCAAATCCTTGAAAAC
TCCCTCTTAAATCTGGAGAGTGGAGACGATGACGGTGTGCAGCTGTCAGACTTCTCTCTCATTATCATTG
ATGAGTGCCATCACACCAACAAGGAGGCAGTCTATAACAACATCATGAGACGATATTTGAAGCAGAAGCT
GAGAAACAATGACCTCAAGAAACAAAACAAACCAGCCATTCCCCTGCCGCAGATACTAGGACTGACAGCT
TCACCTGGTGTTGGAGCAGCCAAAAAGCAGTCTGAGGCTGAAAAACATATTTTAAATATATGTGCCAATC
TTGATGCCTTTACCATTAAAACAGTGAAAGAGAATCTTGGTCAACTCAAACACCAAATAAAGGAACCATG
CAAGAAATTTGTGATTGCTGATGACACCAGAGAAAATCCATTTAAAGAGAAACTTCTAGAAATTATGGCA
AGCATTCAGACTTACTGCCAAAAAAGTCCAATGTCAGATTTTGGAACCCAACATTATGAGCAGTGGGCCA
TTCAAATGGAGAAAAAAGCTGCTAAAGACGGAAATCGCAAAGATCGCGTCTGTGCAGAACATTTGAGGAA
GTACAACGAAGCCCTACAAATCAACGACACGATCCGAATGATTGATGCATATAGCCACCTGGAGACATTC
TACACTGATGAGAAAGAAAAGAAGTTCGCAGTCCTCAATGACAGCGACAAGAGTGATGACGAGGCCAGCA
GTTGCAATGACCAACTTAAGGGCGATGTAAAGAAATCTTTGAAACTGGACGAAACGGATGAATTTCTCAT
GAATTTGTTCTTTGATAACAAGAAAATGTTGAAAAAACTAGCTGAAAACCCAAAATACGAGAATGAAAAA
CTCATTAAATTAAGAAACACGATACTGGAACAATTCACAAGGTCTGAGGAGTCCTCCCGAGGAATTATTT
TTACAAAAACACGACAGAGCACCTACGCACTTTCCCAGTGGATCATGGAAAATGCAAAGTTTGCGGAAGT
TGGAGTCAAAGCGCATCACCTGATTGGCGCGGGGCACAGCAGTGAAGTCAAGCCCATGACTCAGACTGAA
CAAAAAGAAGTCATTAGTAAATTTCGCACTGGCGAAATAAATCTGCTTATCGCTACGACGGTGGCAGAGG
AAGGCCTGGATATCAAAGAGTGCAATATTGTTATTCGTTATGGCCTTGTCACGAACGAGATAGCCATGGT
CCAGGCCCGGGGTCGAGCCAGAGCTGATGAAAGCACGTATGTCCTGGTCACCAGCAGTGGCTCAGGAGTT
ACCGAACGGGAGATTGTTAATGATTTCCGAGAGAAGATGATGTATAAAGCTATTAACCGTGTTCAAAACA
TGAAACCAGAGGAGTATGCACATAAGATTTTGGAATTGCAGGTGCAAAGTATCCTGGAAAAGAAAATGAA
AGTCAAAAGAAGCATTGCAAAGCAATACAACGACAATCCATCGTTAATAACACTTCTCTGCAAAAATTGT
AGCATGCTGGTCTGCTCGGGAGAAAACATCCATGTCATTGAGAAGATGCATCATGTCAATATGACACCAG
AATTCAAGGGACTCTACATTGTAAGAGAAAACAAAGCACTGCAAAAGAAATTTGCTGATTATCAGACCAA
TGGAGAGATTATCTGCAAGTGTGGCCAGGCTTGGGGAACAATGATGGTGCACAAAGGTTTAGATTTGCCT
TGTCTTAAAATAAGGAATTTTGTAGTCAATTTCAAAAATAACTCACCGAAGAAACAGTACAAGAAGTGGG
TGGAATTGCCTATCAGATTTCCTGATCTTGACTACTCAGAATACTGCTTGTATAGTGATGAAGATTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI     
ACCN NM_027835
Insert Size 3078 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_027835.3, NP_082111.2
RefSeq Size 5519 bp
RefSeq ORF 3078 bp
Locus ID 71586
UniProt ID Q8R5F7
Gene Summary Innate immune receptor which acts as a cytoplasmic sensor of viral nucleic acids and plays a major role in sensing viral infection and in the activation of a cascade of antiviral responses including the induction of type I interferons and proinflammatory cytokines. Its ligands include mRNA lacking 2'-O-methylation at their 5' cap and long-dsRNA (>1 kb in length). Upon ligand binding it associates with mitochondria antiviral signaling protein (MAVS/IPS1) which activates the IKK-related kinases: TBK1 and IKBKE which phosphorylate interferon regulatory factors: IRF3 and IRF7 which in turn activate transcription of antiviral immunological genes, including interferons (IFNs); IFN-alpha and IFN-beta. Responsible for detecting the Picornaviridae family members such as encephalomyocarditis virus (EMCV), mengo encephalomyocarditis virus (ENMG), and theiler's murine encephalomyelitis virus (TMEV). Can also detect other viruses such as dengue virus (DENV), west Nile virus (WNV), and reovirus. Also involved in antiviral signaling in response to viruses containing a dsDNA genome, such as vaccinia virus. Plays an important role in amplifying innate immune signaling through recognition of RNA metabolites that are produced during virus infection by ribonuclease L (RNase L). May play an important role in enhancing natural killer cell function and may be involved in growth inhibition and apoptosis in several tumor cell lines.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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