Ptf1a (NM_018809) Mouse Tagged ORF Clone
CAT#: MG226228
- TrueORF®
Ptf1a (tGFP-tagged) - Mouse pancreas specific transcription factor 1a (Ptf1a), (10ug)
ORF Plasmid: DDK
"NM_018809" in other vectors (4)
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CNY 4,000.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | bHLHa29; PTF1-p48; PTF1p48 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_018809 |
ORF Size | 972 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_018809.2, NP_061279.2 |
RefSeq Size | 1508 bp |
RefSeq ORF | 975 bp |
Locus ID | 19213 |
UniProt ID | Q9QX98 |
Gene Summary | Transcription factor implicated in the cell fate determination in various organs. Binds to the E-box consensus sequence 5'-CANNTG-3'. Plays a role in early and late pancreas development and differentiation. Important for determining whether cells allocated to the pancreatic buds continue towards pancreatic organogenesis or revert back to duodenal fates. May be involved in the maintenance of exocrine pancreas-specific gene expression including ELA1 and amylase. Required for the formation of pancreatic acinar and ductal cells. Plays an important role in cerebellar development. Directly regulated by FOXN4 and RORC during retinal development, FOXN4-PTF1A pathway plays a central role in directing the differentiation of retinal progenitors towards horizontal and amacrine fates.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC209252 | Ptf1a (untagged) - Mouse pancreas specific transcription factor, 1a (Ptf1a), (10ug) |
CNY 3,990.00 |
|
MR226228 | Ptf1a (Myc-DDK-tagged) - Mouse pancreas specific transcription factor, 1a (Ptf1a) |
CNY 2,400.00 |
|
MR226228L3 | Lenti ORF clone of Ptf1a (Myc-DDK-tagged) - Mouse pancreas specific transcription factor, 1a (Ptf1a) |
CNY 4,750.00 |
|
MR226228L4 | Lenti ORF clone of Ptf1a (mGFP-tagged) - Mouse pancreas specific transcription factor, 1a (Ptf1a) |
CNY 4,750.00 |