Ptf1a (NM_018809) Mouse Untagged Clone

CAT#: MC209252

Ptf1a (untagged) - Mouse pancreas specific transcription factor, 1a (Ptf1a), (10ug)



  "NM_018809" in other vectors (4)

CNY 3,990.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms bHLHa29; PTF1-p48; PTF1p48
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC209252 representing NM_018809
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACGCCGTACTCCTGGAGCACTTCCCCGGGGGCCTGGACACCTTCCCATCCCCTTACTTTGATGAGG
AAGATTTCTTCACCGACCAGTCCTCTCGGGACCCGCTGGAGGACAGCGACGAGCTGCTGGGGGACGAGCA
AGCAGAAGTAGAGTTCCTCAGCCACCAGCTACACGAATACTGCTACCGCGACGGGGCGTGCCTGCTGCTG
CAACCCGCGCCCTCGGCCGCCCCGCACGCGCTCGCCCCGCCGCCTTTGGGGGATCCTGGCGAGCCCGAGG
ACAACGTCAGCTATTGCTGCGATGCAGGGGCTCCTCTCGCTGCCTTCCCCTACTCGCCTGGCTCACCGCC
CTCGTGCCTCGCCTACCCGTGTGCCGCGGTGCTGTCCCCCGGTGCGCGGCTCGGTGGTTTGAACGGGGCT
GCGGCAGCGGCGGCAGCAAGGCGGCGGCGACGCGTGCGCTCCGAGGCGGAGCTGCAGCAGCTGCGACAAG
CCGCTAATGTGCGAGAGCGGCGCCGCATGCAGTCCATCAACGACGCCTTCGAGGGGCTGCGTTCGCACAT
CCCCACGCTACCCTACGAAAAGCGCCTCTCCAAAGTAGACACGCTGCGCTTGGCCATAGGCTACATTAAC
TTCCTCAGCGAGCTGGTGCAAGCCGACCTGCCGCTGCGCGGGAGTGGCGCAGGTGGTTGCGGGGGCCCAG
GTGGCAGCCGGCACCTCGGAGAGGACAGTCCCGGTAACCAGGCCCAGAAGGTTATCATCTGCCATCGAGG
CACCCGTTCACCCTCCCCCAGTGACCCGGATTATGGTCTCCCTCCTCTTGCAGGGCACTCTCTTTCCTGG
ACTGATGAAAAACAGCTCAAAGAACAAAATATCATCCGTACAGCTAAAGTGTGGACCCCAGAGGACCCCA
GAAAACTCAACAGTAAATCTTTCGACAACATAGAGAACGAACCACCCTTTGAGTTTGTGTCCTGA


AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-RsrII     
ACCN NM_018809
Insert Size 975 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_018809.2, NP_061279.2
RefSeq Size 1508 bp
RefSeq ORF 975 bp
Locus ID 19213
UniProt ID Q9QX98
Gene Summary Transcription factor implicated in the cell fate determination in various organs. Binds to the E-box consensus sequence 5'-CANNTG-3'. Plays a role in early and late pancreas development and differentiation. Important for determining whether cells allocated to the pancreatic buds continue towards pancreatic organogenesis or revert back to duodenal fates. May be involved in the maintenance of exocrine pancreas-specific gene expression including ELA1 and amylase. Required for the formation of pancreatic acinar and ductal cells. Plays an important role in cerebellar development. Directly regulated by FOXN4 and RORC during retinal development, FOXN4-PTF1A pathway plays a central role in directing the differentiation of retinal progenitors towards horizontal and amacrine fates.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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