Neurod2 (NM_010895) Mouse Tagged ORF Clone

CAT#: MG225256

  • TrueORF®

Neurod2 (tGFP-tagged) - Mouse neurogenic differentiation 2 (Neurod2), (10ug)

ORF Plasmid: DDK tGFP



  "NM_010895" in other vectors (6)

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Mouse Protein / Lysate Request

CNY 4,370.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (3)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms bHLHa1; Ndrf
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG225256 representing NM_010895
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGACCCGCCTGTTCAGCGAGCCCGGCCTCCTCTCGGACGTGCCCAAGTTCGCCAGCTGGGGCGACG
GCGACGACGACGAGCCGAGGAGCGACAAGGGCGACGCGCCGCCGCAGCCTCCTCCTGCTCCCGGGTCGGG
GGCTCCAGGACCCGCCCGGGCCGCCAAGCCAGTGTCTCTTCGTGGAGGAGAAGAGATCCCTGAACCCACG
TTGGCTGAGGTCAAGGAGGAAGGAGAGCTGGGCGGCGAGGAGGAGGAGGAAGAGGAGGAGGAGGAAGGAC
TGGACGAGGCGGAAGGCGAGCGGCCCAAGAAGCGCGGGCCGAAGAAACGCAAGATGACCAAGGCGCGTCT
GGAGCGCTCCAAGCTGCGGCGACAGAAGGCCAACGCGCGGGAGCGCAACCGCATGCACGACCTGAACGCG
GCTCTGGACAACCTGCGCAAGGTGGTGCCCTGCTACTCCAAGACGCAGAAGCTGTCCAAGATCGAGACCC
TGCGCCTGGCCAAGAACTACATCTGGGCTCTCTCGGAGATCTTGCGCTCCGGGAAGCGGCCGGATCTGGT
GTCCTACGTGCAGACTCTGTGCAAGGGGCTGTCACAGCCCACCACGAATCTGGTGGCCGGCTGCCTGCAG
CTAAACTCTCGTAACTTCCTCACGGAGCAGGGCGCGGACGGCGCCGGCCGCTTTCACGGCTCGGGTGGCC
CGTTCGCCATGCATCCGTACCCATACCCGTGCTCCCGCCTGGCAGGCGCACAGTGTCAGGCGGCTGGCGG
CCTGGGCGGAGGCGCGGCGCACGCCCTGCGGACCCACGGCTACTGCGCCGCCTACGAGACGCTGTACGCG
GCGGCCGGTGGCGGCGGCGCTAGCCCGGACTACAACAGCTCCGAGTACGAGGGTCCACTCAGTCCCCCGC
TCTGTCTCAACGGCAACTTCTCGCTCAAGCAGGACTCGTCCCCCGATCACGAGAAGAGCTACCACTACTC
TATGCACTACTCGGCGCTGCCCGGCTCACGGCCCACGGGCCACGGGCTGGTCTTCGGCTCGTCGGCCGTG
CGCGGGGGCGTCCACTCCGAGAATCTCTTGTCTTACGATATGCACCTTCACCACGATCGGGGCCCCATGT
ACGAGGAGCTCAACGCATTTTTCCATAAC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG225256 representing NM_010895
Red=Cloning site Green=Tags(s)

MLTRLFSEPGLLSDVPKFASWGDGDDDEPRSDKGDAPPQPPPAPGSGAPGPARAAKPVSLRGGEEIPEPT
LAEVKEEGELGGEEEEEEEEEEGLDEAEGERPKKRGPKKRKMTKARLERSKLRRQKANARERNRMHDLNA
ALDNLRKVVPCYSKTQKLSKIETLRLAKNYIWALSEILRSGKRPDLVSYVQTLCKGLSQPTTNLVAGCLQ
LNSRNFLTEQGADGAGRFHGSGGPFAMHPYPYPCSRLAGAQCQAAGGLGGGAAHALRTHGYCAAYETLYA
AAGGGGASPDYNSSEYEGPLSPPLCLNGNFSLKQDSSPDHEKSYHYSMHYSALPGSRPTGHGLVFGSSAV
RGGVHSENLLSYDMHLHHDRGPMYEELNAFFHN

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_010895
ORF Size 1149 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_010895.3, NP_035025.3
RefSeq Size 3137 bp
RefSeq ORF 1152 bp
Locus ID 18013
UniProt ID Q62414
Gene Summary Transcriptional regulator implicated in neuronal determination. Mediates calcium-dependent transcription activation by binding to E box-containing promoter. Critical factor essential for the repression of the genetic program for neuronal differentiation; prevents the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. Induces transcription of ZEB1, which in turn represses neuronal differentiation by down-regulating REST expression. Plays a role in the establishment and maturation of thalamocortical connections; involved in the segregation of thalamic afferents into distinct barrel domains within layer VI of the somatosensory cortex. Involved in the development of the cerebellar and hippocampal granular neurons, neurons in the basolateral nucleus of amygdala and the hypothalamic-pituitary axis. Associates with chromatin to the DPYSL3 E box-containing promoter.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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