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Limch1 (NM_001001980) Mouse Tagged ORF Clone
CAT＃: MG223602
- TrueORF®
Limch1 (tGFP-tagged) - Mouse LIM and calponin homology domains 1 (Limch1), (10ug)
ORF Plasmid: DDK
"NM_001001980" in other vectors (4)
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CNY 11,590.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 3732412D22Rik; mKIAA1102 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG223602 representing NM_001001980
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTGCCCCGCTCTCGGTCTGGAAGTCCTTCAACCCCTGCAGCCCGAACCACCTCCGGAGCCTGCCT TCGCCGAGGCACAGAAGTGGATCGAGCAAGTCACTGGTCGAAGTTTTGGTGACAAAGACTTCCGGACAGG ACTAGAAAATGGGATCCTCCTTTGCGAGTTGCTGAATGCTATAAAGCCAGGACTTGTTAAAAAGATCAAT AGATTGCCTACCCCCATTGCAGGATTGGACAATACCATCTTATTCTTGAGAGGCTGTAAGGAGCTTGGCC TTAAAGAATCTCAACTCTTTGACCCGAGTGACCTCCAGGATACCTCCAACAGGGTCACTGTCAAGAACCT CGATTATAGCAGGAAGCTGAAAAACGTGCTAGTGACCATTTACTGGCTGGGGAAAGCTGCAAACAGCTGT GCATCCTACGGTGGAACCACACTGAACCTGAAGGAGTTTGAAGGGCTGTTGGCTCAGATGCGAAAGGAGA CTGACGACATTGATAGCCCGAAGCGCAGCATCCGAGACAGTGGCTACATCGACTGCTGGGACTCTGAACG CAGCGACTCCCTCTCTCCTCCTCGCCACGGACGGGATGACTCCTTTGACAGCCTGGATTCTTTTGGCTCC CGCTCCAGGCAGACACCTTCGCCAGATGTAGTTCTCAGGGGAAGCAGTGATGGAAGAGGAAGCGATTCTG AATCAGACTTGCCGCACCGGAAGTTGCCAGATGTGAAGAAAGATGATATGTCCGCACGAAGGACTTCTCA TGGTGAGCCGAAATCAGCAGTGCCTTTTAACCAGTACCTCCCGAACAAAAGCAACCAGACGGCCTACGTC CCGGCGCCTCTGAGAAAGAAGAAGGCGGAGCGGGAGGAATTCAGGAAGAGTTGGAGCACCGCCACCTCCC CCCTGGGTGGGGAAAGGCCCTTCAGATACGGTCCCAGAACTCCTGTGTCAGATGACGCAGAGAGCACGAG TATGTTTGACATGCGGTGTGAGGAGGAGGCAGCGGTGCTGCCGCACAGCAGGGCCCGCCAGGAGCAGCTG CAGCTGATAAATAACCAGCTGAGGGAAGAGGACGACAAATGGCAAGATGACCTGGCTCGCTGGAAGAGCC GGAGAAGAAGCGCTTCTCAGGACTTGATCAAGAAAGAGGAAGAAAGGAAAAAAATGGAGAAGCTGATGTC TGGGGAAGATGGGACAAGTGAGCGGAGGAAAAGCATCAAAACTTACAGAGAAATCGTCCAAGAAAAAGAG CGGAGAGAGAGGGAACTTCATGAGGCGTACAAGAACGCAAGGTCGCAGGAGGAGGCCGAGGGGATCCTCC AGCAGTACATCGAGAGGTTCACCATCAGTGAGGCTGTCCTCGAACGCTTGGAGATGCCAAAAATCCTGGA AAGAAGCCATTCAACAGAGCCAAACGTGTCCTCCTTCCCAAACGACCCCAGCCCCATGAAGTACCTGCGG CAGCAGTCACTGCCCCCACCCAAGTTCACTGCCACGGTTGAGACCACCATAGCTCGCACCAGTGTCCCAG AGAGCATTGCGTCAGCAGGCACTGGGTCTCCAAGCAAAATCATCACTCCAAACACAGTGCCTATGCTGAC ACCCAGGCCTTACTCCCAGCCCAAAAACTCTCAAGAGGTTCTGAAGACTTTTAAGGTAGATGGGAAGGTC AGCATGAATGGGGAAATGGCCCCTGGAGATGAGGAAGGAAAGGAAAAAGAAGGCCCTGCAGCTGCAGCCC CTGGCCCTTCCTTAACCAAGTCCCAGATGTTTGAAGGTGTGGCCACGGTGCACGACTCTCCTGTGCAGGT GAAACAAGGCAGCAACAGCATCGAGATCAACATCAAGAAGCCAAATTCTGCTCCCCAGGAACTGACAGCA GCCTCTGAGGAAACTGAATCGAACGGCCAAGAGGATGAAGACGGTGAAGAAAGGCCAGGGACAGGGGATT TGGAGCCTGACTCGGCAGAACCCCAGCACTTTACGACAACTGTGACTCGGTGCAGCCCAACAGTGGCCTT GGTGGAGTTTTCATCCAACCCGCAGCTGAAGAATGAGGTGCCAGAACAAGGCCAGAAGAAGCCAGAAGAT GAGATGAGTGGGAAGGTGGAGTTAGTGTTGTCACAGAAGGTGGCAAAGCCAAAATCCCCAGAGCCTGAAG CAACCCTGACGTTTCCATTTCTCGACAAAATGCCTGAAACCAACCAACTACATTTGCCAAATCCCAGTTC TCAAGCGGATTCTCCAAGCAGTGAAAAGTCCCCTGGGAGCACACCCTTTAAGTTCTGGGCATGGGACCCA GAAGAGGAGCGCCGGCGACAGGAGAAATGGCAGCAGGAGCAGGAACGTCTGCTGCAGGAGAGGTATCAGA AGGAGCAAGACAAGCTGAAGGAGGAGTGGGAAAAGGCCCAGAAGGAGGTCGAAGAAGAAGAACGCAGATA CTACGAGGAGGAGCGTAAGATAATTGAAGACACGGTGGTTCCATTCACTATTTCCTCAAGCTCTGCAGAC CAACTGTCTACGTCCTTGTCTGTAACTGAAGGCAGCGGGACAAGGAATAAGATGGACTTGGAAAACTGCC CAGACAAAGAAAACGAGAGAAGACAGAAGACGCCTTTCCAGGAGAATGACGGTGATTCATTGCTCAAAAC TAGAGAAGGTGGTCTGCCAGAGGAGCAGAGCCTAACTCCAAGTCCCTCGGCTAACCCTGAAATCTCCGTG TCAAAAGGAATCCATCAGGACCCGCAGCTGGAGGCAGAGGCTGGGGCCCCACACTGTGGTACAAACCCAC AGCCAGCTCAGGATCCACCTCGGAATCAACAGATACCAAACCCACCAACATCCACGTCGGAAGATGTGAA GCCCAAAACCTTGGCCCTGGAGAAAACCATCAATCATCAGATGGAGTCTCCGGGGGAAAGGCGGAAGTCT ATAAGCGGGAAGAAACTGTGCTCCTCCTGCGGGCTCACTTTGGGTAAAGGTGCTGCGATGATCATCGAGA CCCTGAATCTCTACTTTCACATTCAGTGTTTCAGGTGCGGCATCTGTAAAGGACAGCTCGGAGATGCAGT AAGCGGGACAGACGTCAGGATTCGCAATGGTCTCCTAAACTGTACCGACTGCTACATGCGATCCAGAAGT GCCGGCCAGCCTACAACACTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG223602 representing NM_001001980
Red=Cloning site Green=Tags(s) MACPALGLEVLQPLQPEPPPEPAFAEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKIN RLPTPIAGLDNTILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKNLDYSRKLKNVLVTIYWLGKAANSC ASYGGTTLNLKEFEGLLAQMRKETDDIDSPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGS RSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYV PAPLRKKKAEREEFRKSWSTATSPLGGERPFRYGPRTPVSDDAESTSMFDMRCEEEAAVLPHSRARQEQL QLINNQLREEDDKWQDDLARWKSRRRSASQDLIKKEEERKKMEKLMSGEDGTSERRKSIKTYREIVQEKE RRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNVSSFPNDPSPMKYLR QQSLPPPKFTATVETTIARTSVPESIASAGTGSPSKIITPNTVPMLTPRPYSQPKNSQEVLKTFKVDGKV SMNGEMAPGDEEGKEKEGPAAAAPGPSLTKSQMFEGVATVHDSPVQVKQGSNSIEINIKKPNSAPQELTA ASEETESNGQEDEDGEERPGTGDLEPDSAEPQHFTTTVTRCSPTVALVEFSSNPQLKNEVPEQGQKKPED EMSGKVELVLSQKVAKPKSPEPEATLTFPFLDKMPETNQLHLPNPSSQADSPSSEKSPGSTPFKFWAWDP EEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTISSSSAD QLSTSLSVTEGSGTRNKMDLENCPDKENERRQKTPFQENDGDSLLKTREGGLPEEQSLTPSPSANPEISV SKGIHQDPQLEAEAGAPHCGTNPQPAQDPPRNQQIPNPPTSTSEDVKPKTLALEKTINHQMESPGERRKS ISGKKLCSSCGLTLGKGAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCTDCYMRSRS AGQPTTL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001001980 |
| ORF Size | 3171 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001001980.3 |
| RefSeq Size | 6142 bp |
| RefSeq ORF | 3174 bp |
| Locus ID | 77569 |
| UniProt ID | Q3UH68 |
| Gene Summary | Actin stress fibers-associated protein that activates non-muscle myosin IIa. Activates the non-muscle myosin IIa complex by promoting the phosphorylation of its regulatory subunit MRLC/MYL9. Through the activation of non-muscle myosin IIa, positively regulates actin stress fibers assembly and stabilizes focal adhesions. It therefore negatively regulates cell spreading and cell migration.[UniProtKB/Swiss-Prot Function] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223397 | Limch1 (untagged) - Mouse LIM and calponin homology domains 1 (Limch1), (10ug) |
CNY 13,590.00 |
|
| MR223602 | Limch1 (Myc-DDK-tagged) - Mouse LIM and calponin homology domains 1 (Limch1) |
CNY 10,550.00 |
|
| MR223602L3 | Lenti ORF clone of Limch1 (Myc-DDK-tagged) - Mouse LIM and calponin homology domains 1 (Limch1) |
CNY 12,450.00 |
|
| MR223602L4 | Lenti ORF clone of Limch1 (mGFP-tagged) - Mouse LIM and calponin homology domains 1 (Limch1) |
CNY 12,450.00 |
