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Limch1 (NM_001001980) Mouse Untagged Clone
CAT＃: MC223397
Limch1 (untagged) - Mouse LIM and calponin homology domains 1 (Limch1), (10ug)
CNY 13,590.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 3732412D22Rik; mKIAA1102 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC223397 representing NM_001001980
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTGCCCCGCTCTCGGTCTGGAAGTCCTTCAACCCCTGCAGCCCGAACCACCTCCGGAGCCTGCCT TCGCCGAGGCACAGAAGTGGATCGAGCAAGTCACTGGTCGAAGTTTTGGTGACAAAGACTTCCGGACAGG ACTAGAAAATGGGATCCTCCTTTGCGAGTTGCTGAATGCTATAAAGCCAGGACTTGTTAAAAAGATCAAT AGATTGCCTACCCCCATTGCAGGATTGGACAATACCATCTTATTCTTGAGAGGCTGTAAGGAGCTTGGCC TTAAAGAATCTCAACTCTTTGACCCGAGTGACCTCCAGGATACCTCCAACAGGGTCACTGTCAAGAACCT CGATTATAGCAGGAAGCTGAAAAACGTGCTAGTGACCATTTACTGGCTGGGGAAAGCTGCAAACAGCTGT GCATCCTACGGTGGAACCACACTGAACCTGAAGGAGTTTGAAGGGCTGTTGGCTCAGATGCGAAAGGAGA CTGACGACATTGATAGCCCGAAGCGCAGCATCCGAGACAGTGGCTACATCGACTGCTGGGACTCTGAACG CAGCGACTCCCTCTCTCCTCCTCGCCACGGACGGGATGACTCCTTTGACAGCCTGGATTCTTTTGGCTCC CGCTCCAGGCAGACACCTTCGCCAGATGTAGTTCTCAGGGGAAGCAGTGATGGAAGAGGAAGCGATTCTG AATCAGACTTGCCGCACCGGAAGTTGCCAGATGTGAAGAAAGATGATATGTCCGCACGAAGGACTTCTCA TGGTGAGCCGAAATCAGCAGTGCCTTTTAACCAGTACCTCCCGAACAAAAGCAACCAGACGGCCTACGTC CCGGCGCCTCTGAGAAAGAAGAAGGCGGAGCGGGAGGAATTCAGGAAGAGTTGGAGCACCGCCACCTCCC CCCTGGGTGGGGAAAGGCCCTTCAGATACGGTCCCAGAACTCCTGTGTCAGATGACGCAGAGAGCACGAG TATGTTTGACATGCGGTGTGAGGAGGAGGCAGCGGTGCTGCCGCACAGCAGGGCCCGCCAGGAGCAGCTG CAGCTGATAAATAACCAGCTGAGGGAAGAGGACGACAAATGGCAAGATGACCTGGCTCGCTGGAAGAGCC GGAGAAGAAGCGCTTCTCAGGACTTGATCAAGAAAGAGGAAGAAAGGAAAAAAATGGAGAAGCTGATGTC TGGGGAAGATGGGACAAGTGAGCGGAGGAAAAGCATCAAAACTTACAGAGAAATCGTCCAAGAAAAAGAG CGGAGAGAGAGGGAACTTCATGAGGCGTACAAGAACGCAAGGTCGCAGGAGGAGGCCGAGGGGATCCTCC AGCAGTACATCGAGAGGTTCACCATCAGTGAGGCTGTCCTCGAACGCTTGGAGATGCCAAAAATCCTGGA AAGAAGCCATTCAACAGAGCCAAACGTGTCCTCCTTCCCAAACGACCCCAGCCCCATGAAGTACCTGCGG CAGCAGTCACTGCCCCCACCCAAGTTCACTGCCACGGTTGAGACCACCATAGCTCGCACCAGTGTCCCAG AGAGCATTGCGTCAGCAGGCACTGGGTCTCCAAGCAAAATCATCACTCCAAACACAGTGCCTATGCTGAC ACCCAGGCCTTACTCCCAGCCCAAAAACTCTCAAGAGGTTCTGAAGACTTTTAAGGTAGATGGGAAGGTC AGCATGAATGGGGAAATGGCCCCTGGAGATGAGGAAGGAAAGGAAAAAGAAGGCCCTGCAGCTGCAGCCC CTGGCCCTTCCTTAACCAAGTCCCAGATGTTTGAAGGTGTGGCCACGGTGCACGACTCTCCTGTGCAGGT GAAACAAGGCAGCAACAGCATCGAGATCAACATCAAGAAGCCAAATTCTGCTCCCCAGGAACTGACAGCA GCCTCTGAGGAAACTGAATCGAACGGCCAAGAGGATGAAGACGGTGAAGAAAGGCCAGGGACAGGGGATT TGGAGCCTGACTCGGCAGAACCCCAGCACTTTACGACAACTGTGACTCGGTGCAGCCCAACAGTGGCCTT GGTGGAGTTTTCATCCAACCCGCAGCTGAAGAATGAGGTGCCAGAACAAGGCCAGAAGAAGCCAGAAGAT GAGATGAGTGGGAAGGTGGAGTTAGTGTTGTCACAGAAGGTGGCAAAGCCAAAATCCCCAGAGCCTGAAG CAACCCTGACGTTTCCATTTCTCGACAAAATGCCTGAAACCAACCAACTACATTTGCCAAATCCCAGTTC TCAAGCGGATTCTCCAAGCAGTGAAAAGTCCCCTGGGAGCACACCCTTTAAGTTCTGGGCATGGGACCCA GAAGAGGAGCGCCGGCGACAGGAGAAATGGCAGCAGGAGCAGGAACGTCTGCTGCAGGAGAGGTATCAGA AGGAGCAAGACAAGCTGAAGGAGGAGTGGGAAAAGGCCCAGAAGGAGGTCGAAGAAGAAGAACGCAGATA CTACGAGGAGGAGCGTAAGATAATTGAAGACACGGTGGTTCCATTCACTATTTCCTCAAGCTCTGCAGAC CAACTGTCTACGTCCTTGTCTGTAACTGAAGGCAGCGGGACAAGGAATAAGATGGACTTGGAAAACTGCC CAGACAAAGAAAACGAGAGAAGACAGAAGACGCCTTTCCAGGAGAATGACGGTGATTCATTGCTCAAAAC TAGAGAAGGTGGTCTGCCAGAGGAGCAGAGCCTAACTCCAAGTCCCTCGGCTAACCCTGAAATCTCCGTG TCAAAAGGAATCCATCAGGACCCGCAGCTGGAGGCAGAGGCTGGGGCCCCACACTGTGGTACAAACCCAC AGCCAGCTCAGGATCCACCTCGGAATCAACAGATACCAAACCCACCAACATCCACGTCGGAAGATGTGAA GCCCAAAACCTTGGCCCTGGAGAAAACCATCAATCATCAGATGGAGTCTCCGGGGGAAAGGCGGAAGTCT ATAAGCGGGAAGAAACTGTGCTCCTCCTGCGGGCTCACTTTGGGTAAAGGTGCTGCGATGATCATCGAGA CCCTGAATCTCTACTTTCACATTCAGTGTTTCAGGTGCGGCATCTGTAAAGGACAGCTCGGAGATGCAGT AAGCGGGACAGACGTCAGGATTCGCAATGGTCTCCTAAACTGTACCGACTGCTACATGCGATCCAGAAGT GCCGGCCAGCCTACAACACTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001001980 |
| Insert Size | 3174 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001001980.2, NP_001001980.2 |
| RefSeq Size | 6142 bp |
| RefSeq ORF | 3174 bp |
| Locus ID | 77569 |
| UniProt ID | Q3UH68 |
| Gene Summary | Actin stress fibers-associated protein that activates non-muscle myosin IIa. Activates the non-muscle myosin IIa complex by promoting the phosphorylation of its regulatory subunit MRLC/MYL9. Through the activation of non-muscle myosin IIa, positively regulates actin stress fibers assembly and stabilizes focal adhesions. It therefore negatively regulates cell spreading and cell migration.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG223602 | Limch1 (tGFP-tagged) - Mouse LIM and calponin homology domains 1 (Limch1), (10ug) |
CNY 11,590.00 |
|
| MR223602 | Limch1 (Myc-DDK-tagged) - Mouse LIM and calponin homology domains 1 (Limch1) |
CNY 10,550.00 |
|
| MR223602L3 | Lenti ORF clone of Limch1 (Myc-DDK-tagged) - Mouse LIM and calponin homology domains 1 (Limch1) |
CNY 12,450.00 |
|
| MR223602L4 | Lenti ORF clone of Limch1 (mGFP-tagged) - Mouse LIM and calponin homology domains 1 (Limch1) |
CNY 12,450.00 |
