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Ect2 (NM_007900) Mouse Tagged ORF Clone
CAT＃: MG223423
- TrueORF®
Ect2 (tGFP-tagged) - Mouse ect2 oncogene (Ect2) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_007900" in other vectors (4)
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CNY 10,070.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AI528536; mKIAA4037 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG223423 representing NM_007900
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGACGACAGCGTGTTACCATCTCCTTCTGAGATAACTAGCTTGGCAGACTCTTCAGTGTTTGATT CTAAAGTTGCTGAAATGTCCAAGGAAAACTTGTGCTTGGCGTCTACTTCAAATGTTGATGAAGAAATGCC GCAGGTTGAAGCAAGAGTGATAATGGTCCAGGATGCTGGGAAACAAGAAGAACTTCTAAAGGCCTTAAAG ACTATTAAGATAATGGAAGTCCCTGTTATAAAGATAAAAGAAAGTTGTCCTGGAAAATCGGAGGAAAAAT TAATAAAAAGTATTATTAATATGGAAATGAAAGTGCCCTGTGTAAAGATGGACTCAATGGAAGAATTTGA AAGTTTGGATTCCCCAGAATTTGAGAATATATTTGTAGTTACTGACTTCCAGAATTCTGTCTTCAATGAC TTATACAAGGCTGATTGTAGGATTGTTGGGCCACCAGTTATACTCAACTGTGCACAAAGGGGAGAGCCCT TGCCGTTTTCCTGCCGGCCGCTGTATTGTACGAGTATGCTGAACCTGGTGCTCTGCTTCACTGGATTCAG GAAGAAGGAGGAGCTTGTCAAATTGGTGACGTTGGTTCATCATATGGGTGGAGTTATTCGAAAAGAGTGT AATTCCAAAGTAACACATCTGGTGGCAAATTGTACACAAGGCGAAAAATTTAGGGTTGCTGTGAGCCTGG GCACTCCAATTATGAAGCCAGAATGGATTTATAAAGCGTGGGAAAGACGCAATGAACAGTGTTTCTGTGC AGCAGTTGATGACTTTAGAAATGAATTTAAAGTTCCTCCATTCCAAGATTGCATTTTAAGTTTCCTGGGA TTTTCAGATGAAGAGAAACATAGTATGGAAGAAATGACTGAAATGCAAGGAGGTAGCTATTTACCAGTTG GGGATGAAAGGTGCACTCACCTTATTGTTGAAGAGAATACAGTAAAGGACCTTCCATTCGAACCTTCAAA GAAACTTTTTGTTGTCAAGCAAGAGTGGTTCTGGGGAAGCATTCAGATGGACGCTCGTGCAGGAGAGACT ATGTATTTGTATGAAAAGGCTAATACTCCTGAGCTCAAGAAATCGGTGTCTCTGCTTTCTCTAAGTACTC CAAACAGCAACCGCAAGAGACGGAGATTGAAAGAGACCCTGGCTCAGCTCTCCAGGGAGACTGACCTCTC TCCTTTCCCTCCCCGGAAGCGGCCCTCAGCTGAGCACTCTCTTTCTATTGGCTCACTCCTGGATATCTCC AACACACCCGAGTCCAGCATCCACTATGGAGAAACACCGAAGTCCTGTGCTAAGTCTTCTAGAAGTTCTA CTCCAGTTCCTCCAAAGCAGTCAGCCAGGTGGCAGGTGGCGAAAGAGCTCTACCAGACTGAGAGCAATTA TGTCAATATACTGGCCACAATCATTCAATTATTTCAGGTGCCCTTAGAAGAAGAAGGACAGCGTGGTGGG CCTATTCTTGCACCTGAGGAGATCAAGACTATTTTTGGGAGCATCCCAGATATCTTTGATGTGCATATGA AGATCAAGGATGATCTTGAAGACCTTATTGCTAACTGGGATGAGAGCAGAAGTATTGGTGACATCTTTCT TAAATATGCAAAAGATTTGGTAAAAACCTACCCTCCGTTTGTAAACTTCTTTGAAATGAGCAAGGAAATG ATCATTAAATGTGAGAAACAAAAGCCCAGATTTCATGCTTTCCTCAAGATAAATCAAGCTAAACCAGAAT GTGGACGACAGAGCCTTGTGGAACTTCTCATCCGGCCAGTGCAGAGGCTACCCAGTGTCGCTTTACTTTT AAACGATCTTAAAAAGCATACAGCTGATGAAAATCCAGACAAAAGCACTTTAGAAAAAGCTATTGGATCA CTAAAAGAAGTAATGACACATATCAATGAGGATAAGAGAAAAACAGAAGCACAGAAGCAAATTTTTGATG TTGTTTATGAAGTAGATGGATGCCCAGCTAATCTCTTATCTTCTCATCGGAGCCTGGTGCAACGAGTGGA AACGGTTTCCCTTGGTGAGCACCCTTGCGACCGAGGAGAACAAGTCACTCTCTTCCTCTTCAATGACTGC CTCGAGATAGCAAGAAAGCGGCACAAGGTTATTGGCACTTTTAGAAGTCCTCACGACCGCACCCGGCCCC CCGCTTCTCTGAAGCACATTCATCTCATGCCTCTTTCTCAGATTAAGAAGGTGCTGGACATCCGAGAGAC AGAAGATTGTCACAATGCCTTTGCCTTGCTTGTAAGGCCACCAACAGAACAGGCCAATGTACTGCTCAGC TTCCAGATGACGTCAGAGGAGCTTCCAAAGGAGAGCTGGCTGAAGATGCTGTGCCGACATGTAGCCAACA CCATTTGTAAGGCAGATGCTGAGAATCTTATGTATGTGGCTGATCCAGAATCCTTTGAAGTAAATACAAA AGATATGGATAGTACATTGAGTCGAGCATCTAGAGCAATAAAGAAGACTTCAAAAAAGGTTACAAGGGCA TTCTCTTTTTCCAAAACTCCCAAGAGAGCCCTGCGGATGGCTCTTTCATCATCCCATAGCTCAGAGGGAA GGAGTCCTCCGAGCAGTGGCAAGCTTGCTGTGAGCCGTCTGTCCAGCACATCATCCTTAGCAGGTATTCC ATCTCCCTCCCTTGTCAGCCTCCCTTCTTTCTTTGAACGGAGAAGTCATACATTAAGTCGATCTACAACT CACTTGATA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG223423 representing NM_007900
Red=Cloning site Green=Tags(s) MADDSVLPSPSEITSLADSSVFDSKVAEMSKENLCLASTSNVDEEMPQVEARVIMVQDAGKQEELLKALK TIKIMEVPVIKIKESCPGKSEEKLIKSIINMEMKVPCVKMDSMEEFESLDSPEFENIFVVTDFQNSVFND LYKADCRIVGPPVILNCAQRGEPLPFSCRPLYCTSMLNLVLCFTGFRKKEELVKLVTLVHHMGGVIRKEC NSKVTHLVANCTQGEKFRVAVSLGTPIMKPEWIYKAWERRNEQCFCAAVDDFRNEFKVPPFQDCILSFLG FSDEEKHSMEEMTEMQGGSYLPVGDERCTHLIVEENTVKDLPFEPSKKLFVVKQEWFWGSIQMDARAGET MYLYEKANTPELKKSVSLLSLSTPNSNRKRRRLKETLAQLSRETDLSPFPPRKRPSAEHSLSIGSLLDIS NTPESSIHYGETPKSCAKSSRSSTPVPPKQSARWQVAKELYQTESNYVNILATIIQLFQVPLEEEGQRGG PILAPEEIKTIFGSIPDIFDVHMKIKDDLEDLIANWDESRSIGDIFLKYAKDLVKTYPPFVNFFEMSKEM IIKCEKQKPRFHAFLKINQAKPECGRQSLVELLIRPVQRLPSVALLLNDLKKHTADENPDKSTLEKAIGS LKEVMTHINEDKRKTEAQKQIFDVVYEVDGCPANLLSSHRSLVQRVETVSLGEHPCDRGEQVTLFLFNDC LEIARKRHKVIGTFRSPHDRTRPPASLKHIHLMPLSQIKKVLDIRETEDCHNAFALLVRPPTEQANVLLS FQMTSEELPKESWLKMLCRHVANTICKADAENLMYVADPESFEVNTKDMDSTLSRASRAIKKTSKKVTRA FSFSKTPKRALRMALSSSHSSEGRSPPSSGKLAVSRLSSTSSLAGIPSPSLVSLPSFFERRSHTLSRSTT HLI TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_007900 |
| ORF Size | 2739 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_007900.3, NP_031926.2 |
| RefSeq Size | 4095 bp |
| RefSeq ORF | 2742 bp |
| Locus ID | 13605 |
| UniProt ID | Q07139 |
| Gene Summary | Guanine nucleotide exchange factor (GEF) that catalyzes the exchange of GDP for GTP. Promotes guanine nucleotide exchange on the Rho family members of small GTPases, like RHOA, RHOC, RAC1 and CDC42. Required for signal transduction pathways involved in the regulation of cytokinesis. Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Regulates the translocation of RHOA from the central spindle to the equatorial region. Plays a role in the control of mitotic spindle assembly; regulates the activation of CDC42 in metaphase for the process of spindle fibers attachment to kinetochores before chromosome congression. Involved in the regulation of epithelial cell polarity; participates in the formation of epithelial tight junctions in a polarity complex PARD3-PARD6-protein kinase PRKCQ-dependent manner. Plays a role in the regulation of neurite outgrowth. Inhibits phenobarbital (PB)-induced NR1I3 nuclear translocation. Stimulates the activity of RAC1 through its association with the oncogenic PARD6A-PRKCI complex in cancer cells, thereby acting to coordinately drive tumor cell proliferation and invasion. Also stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death.[UniProtKB/Swiss-Prot Function] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222574 | Ect2 (untagged) - Mouse ect2 oncogene (Ect2), transcript variant 1, (10ug) |
CNY 11,780.00 |
|
| MR223423 | Ect2 (Myc-DDK-tagged) - Mouse ect2 oncogene (Ect2), transcript variant 1 |
CNY 9,120.00 |
|
| MR223423L3 | Lenti ORF clone of Ect2 (Myc-DDK-tagged) - Mouse ect2 oncogene (Ect2), transcript variant 1 |
CNY 11,020.00 |
|
| MR223423L4 | Lenti ORF clone of Ect2 (mGFP-tagged) - Mouse ect2 oncogene (Ect2), transcript variant 1 |
CNY 11,020.00 |
