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Ect2 (NM_007900) Mouse Untagged Clone
CAT＃: MC222574
Ect2 (untagged) - Mouse ect2 oncogene (Ect2), transcript variant 1, (10ug)
CNY 11,780.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | AI528536; mKIAA4037 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC222574 representing NM_007900
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGACGACAGCGTGTTACCATCTCCTTCTGAGATAACTAGCTTGGCAGACTCTTCAGTGTTTGATT CTAAAGTTGCTGAAATGTCCAAGGAAAACTTGTGCTTGGCGTCTACTTCAAATGTTGATGAAGAAATGCC GCAGGTTGAAGCAAGAGTGATAATGGTCCAGGATGCTGGGAAACAAGAAGAACTTCTAAAGGCCTTAAAG ACTATTAAGATAATGGAAGTCCCTGTTATAAAGATAAAAGAAAGTTGTCCTGGAAAATCGGAGGAAAAAT TAATAAAAAGTATTATTAATATGGAAATGAAAGTGCCCTGTGTAAAGATGGACTCAATGGAAGAATTTGA AAGTTTGGATTCCCCAGAATTTGAGAATATATTTGTAGTTACTGACTTCCAGAATTCTGTCTTCAATGAC TTATACAAGGCTGATTGTAGGATTGTTGGGCCACCAGTTATACTCAACTGTGCACAAAGGGGAGAGCCCT TGCCGTTTTCCTGCCGGCCGCTGTATTGTACGAGTATGCTGAACCTGGTGCTCTGCTTCACTGGATTCAG GAAGAAGGAGGAGCTTGTCAAATTGGTGACGTTGGTTCATCATATGGGTGGAGTTATTCGAAAAGAGTGT AATTCCAAAGTAACACATCTGGTGGCAAATTGTACACAAGGCGAAAAATTTAGGGTTGCTGTGAGCCTGG GCACTCCAATTATGAAGCCAGAATGGATTTATAAAGCGTGGGAAAGACGCAATGAACAGTGTTTCTGTGC AGCAGTTGATGACTTTAGAAATGAATTTAAAGTTCCTCCATTCCAAGATTGCATTTTAAGTTTCCTGGGA TTTTCAGATGAAGAGAAACATAGTATGGAAGAAATGACTGAAATGCAAGGAGGTAGCTATTTACCAGTTG GGGATGAAAGGTGCACTCACCTTATTGTTGAAGAGAATACAGTAAAGGACCTTCCATTCGAACCTTCAAA GAAACTTTTTGTTGTCAAGCAAGAGTGGTTCTGGGGAAGCATTCAGATGGACGCTCGTGCAGGAGAGACT ATGTATTTGTATGAAAAGGCTAATACTCCTGAGCTCAAGAAATCGGTGTCTCTGCTTTCTCTAAGTACTC CAAACAGCAACCGCAAGAGACGGAGATTGAAAGAGACCCTGGCTCAGCTCTCCAGGGAGACTGACCTCTC TCCTTTCCCTCCCCGGAAGCGGCCCTCAGCTGAGCACTCTCTTTCTATTGGCTCACTCCTGGATATCTCC AACACACCCGAGTCCAGCATCCACTATGGAGAAACACCGAAGTCCTGTGCTAAGTCTTCTAGAAGTTCTA CTCCAGTTCCTCCAAAGCAGTCAGCCAGGTGGCAGGTGGCGAAAGAGCTCTACCAGACTGAGAGCAATTA TGTCAATATACTGGCCACAATCATTCAATTATTTCAGGTGCCCTTAGAAGAAGAAGGACAGCGTGGTGGG CCTATTCTTGCACCTGAGGAGATCAAGACTATTTTTGGGAGCATCCCAGATATCTTTGATGTGCATATGA AGATCAAGGATGATCTTGAAGACCTTATTGCTAACTGGGATGAGAGCAGAAGTATTGGTGACATCTTTCT TAAATATGCAAAAGATTTGGTAAAAACCTACCCTCCGTTTGTAAACTTCTTTGAAATGAGCAAGGAAATG ATCATTAAATGTGAGAAACAAAAGCCCAGATTTCATGCTTTCCTCAAGATAAATCAAGCTAAACCAGAAT GTGGACGACAGAGCCTTGTGGAACTTCTCATCCGGCCAGTGCAGAGGCTACCCAGTGTCGCTTTACTTTT AAACGATCTTAAAAAGCATACAGCTGATGAAAATCCAGACAAAAGCACTTTAGAAAAAGCTATTGGATCA CTAAAAGAAGTAATGACACATATCAATGAGGATAAGAGAAAAACAGAAGCACAGAAGCAAATTTTTGATG TTGTTTATGAAGTAGATGGATGCCCAGCTAATCTCTTATCTTCTCATCGGAGCCTGGTGCAACGAGTGGA AACGGTTTCCCTTGGTGAGCACCCTTGCGACCGAGGAGAACAAGTCACTCTCTTCCTCTTCAATGACTGC CTCGAGATAGCAAGAAAGCGGCACAAGGTTATTGGCACTTTTAGAAGTCCTCACGACCGCACCCGGCCCC CCGCTTCTCTGAAGCACATTCATCTCATGCCTCTTTCTCAGATTAAGAAGGTGCTGGACATCCGAGAGAC AGAAGATTGTCACAATGCCTTTGCCTTGCTTGTAAGGCCACCAACAGAACAGGCCAATGTACTGCTCAGC TTCCAGATGACGTCAGAGGAGCTTCCAAAGGAGAGCTGGCTGAAGATGCTGTGCCGACATGTAGCCAACA CCATTTGTAAGGCAGATGCTGAGAATCTTATGTATGTGGCTGATCCAGAATCCTTTGAAGTAAATACAAA AGATATGGATAGTACATTGAGTCGAGCATCTAGAGCAATAAAGAAGACTTCAAAAAAGGTTACAAGGGCA TTCTCTTTTTCCAAAACTCCCAAGAGAGCCCTGCGGATGGCTCTTTCATCATCCCATAGCTCAGAGGGAA GGAGTCCTCCGAGCAGTGGCAAGCTTGCTGTGAGCCGTCTGTCCAGCACATCATCCTTAGCAGGTATTCC ATCTCCCTCCCTTGTCAGCCTCCCTTCTTTCTTTGAACGGAGAAGTCATACATTAAGTCGATCTACAACT CACTTGATATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_007900 |
| Insert Size | 2742 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_007900.3, NP_031926.2 |
| RefSeq Size | 4095 bp |
| RefSeq ORF | 2742 bp |
| Locus ID | 13605 |
| UniProt ID | Q07139 |
| Gene Summary | Guanine nucleotide exchange factor (GEF) that catalyzes the exchange of GDP for GTP. Promotes guanine nucleotide exchange on the Rho family members of small GTPases, like RHOA, RHOC, RAC1 and CDC42. Required for signal transduction pathways involved in the regulation of cytokinesis. Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Regulates the translocation of RHOA from the central spindle to the equatorial region. Plays a role in the control of mitotic spindle assembly; regulates the activation of CDC42 in metaphase for the process of spindle fibers attachment to kinetochores before chromosome congression. Involved in the regulation of epithelial cell polarity; participates in the formation of epithelial tight junctions in a polarity complex PARD3-PARD6-protein kinase PRKCQ-dependent manner. Plays a role in the regulation of neurite outgrowth. Inhibits phenobarbital (PB)-induced NR1I3 nuclear translocation. Stimulates the activity of RAC1 through its association with the oncogenic PARD6A-PRKCI complex in cancer cells, thereby acting to coordinately drive tumor cell proliferation and invasion. Also stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) encodes the longer isoform (1). CCDS Note: The coding region has been updated to an alternate splice pattern, which adds an in-frame coding exon, and extends the N-terminus to one that is more supported by available conservation data. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG223423 | Ect2 (tGFP-tagged) - Mouse ect2 oncogene (Ect2) transcript variant 1, (10ug) |
CNY 10,070.00 |
|
| MR223423 | Ect2 (Myc-DDK-tagged) - Mouse ect2 oncogene (Ect2), transcript variant 1 |
CNY 9,120.00 |
|
| MR223423L3 | Lenti ORF clone of Ect2 (Myc-DDK-tagged) - Mouse ect2 oncogene (Ect2), transcript variant 1 |
CNY 11,020.00 |
|
| MR223423L4 | Lenti ORF clone of Ect2 (mGFP-tagged) - Mouse ect2 oncogene (Ect2), transcript variant 1 |
CNY 11,020.00 |
