United States
Germany
Japan
United Kingdom
China
Bmp3 (NM_173404) Mouse Tagged ORF Clone
CAT＃: MG223213
- TrueORF®
Bmp3 (tGFP-tagged) - Mouse bone morphogenetic protein 3 (Bmp3), (10ug)
ORF Plasmid: DDK
"NM_173404" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 3,710.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 9530029I04Rik; D630004D15R |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG223213 representing NM_173404
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGGGGCGCGCGGGCTGCTCTGTCTATGGCTAGGTTACTTCTGCCTGAACCTGGCACAGGGACAGA GACCAAACTTGCACCTCCCGGGACTCCGTGAGACTGAGCCAAGCGACCGCGCGACAGGTGGTAGCCCGAG TCCGGACCTGAGGCCGCACGACAAGGTGTCGGAGCATATGCTGTGGCTCTATGACAGGTACAGCGGCAGC AGTAGAGTCCAGGCTACCCGGACACCGGGCTCGCAGCTCCCGGGTCCTCAGCCCCTGCGCGGTGGTAACA CGGTCCGCAGCTTCAGAGCCGCAGCCGCAGGAACTCCTCAAACGAAGGGGTTACACACCTTCAATCTGAC TTCTCTAACCAAGTCGGAGAACATTTTGTCAGCCACACTGTATTTCTACGTTGGAGAATTAGTGAACATC AGCTTGAGCTGTCCAGAACCCCAAGGATGCTCCCATCACACTCAGAGACAACACATCCAGATAGACCTCT CTGCATGGATCCTCAAATCCAACCAAAGCCAGCTCTTGGGTCATCTGTCTGTAGATGTGGTCAGACCTTA TAGAGACAGCGTGTCTTGGCTGTCAAAAGACATCACTCAGCTCTTAAGAAAGGCCAAGCAAAATGAAGAG TTTCTCATAGGGTTTAACATTACCTCCAGAGCACACGAGCTACCCAAGAGGATGCTGTTTTTCCCGGAAC CTTATATCTTGGTATACGCCAACGATGCTGCCATTTCTGAGCCAGAAAGCGTGGTATCCAGCTTACAGAG ACACCGAGATTTCACAGCGGGAACTGGGCCCAGACTGGATAGCCACGTCAGAGAAGCCCTCTCGGTTGAG AGGAGGAAGAAGCGCTCTACTGGGATCTTGCTGCCCTTGCAGAACAATGAGCTACCTGGGGCAGAGTATC AGTACAAGGAGGAGGGAGCGTGGGAGGAGAGAAAGCCTTATAAGAGCCTTCAGACTCAGCCCCCTGAGAA GAGTAGGAACAAAAAGAAACAGAGGAAAGGGTCCCATCAGAAGGGACAGACGCTGCAATTTGATGAGCAG ACCCTGAAGAAGGCAAGGCGAAAGCAGTGGGTCGAACCTCGGAACTGTGCCAGGAGGTACCTTAAAGTGG ACTTTGCTGATATCGGCTGGAGCGAATGGATTATCTCTCCCAAGTCATTTGATGCTTTCTACTGCTCTGG AGCCTGCCAGTTCCCCATGCCAAAGTCTTTGAAACCATCAAATCACGCCACCATCCAGAGCATAGTGCGA GCGGTGGGGGTCGTCTCCGGGATTCCCGAGCCTTGCTGTGTGCCGGAAAAGATGTCCTCACTCAGCATCT TGTTCTTTGATGAAAACAAGAATGTAGTGCTCAAAGTCTACCCTAACATGACAGTCGACTCCTGTGCTTG TAGA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG223213 representing NM_173404
Red=Cloning site Green=Tags(s) MAGARGLLCLWLGYFCLNLAQGQRPNLHLPGLRETEPSDRATGGSPSPDLRPHDKVSEHMLWLYDRYSGS SRVQATRTPGSQLPGPQPLRGGNTVRSFRAAAAGTPQTKGLHTFNLTSLTKSENILSATLYFYVGELVNI SLSCPEPQGCSHHTQRQHIQIDLSAWILKSNQSQLLGHLSVDVVRPYRDSVSWLSKDITQLLRKAKQNEE FLIGFNITSRAHELPKRMLFFPEPYILVYANDAAISEPESVVSSLQRHRDFTAGTGPRLDSHVREALSVE RRKKRSTGILLPLQNNELPGAEYQYKEEGAWEERKPYKSLQTQPPEKSRNKKKQRKGSHQKGQTLQFDEQ TLKKARRKQWVEPRNCARRYLKVDFADIGWSEWIISPKSFDAFYCSGACQFPMPKSLKPSNHATIQSIVR AVGVVSGIPEPCCVPEKMSSLSILFFDENKNVVLKVYPNMTVDSCACR TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_173404 |
| ORF Size | 1404 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_173404.5, NP_775580.1 |
| RefSeq Size | 3112 bp |
| RefSeq ORF | 1407 bp |
| Locus ID | 110075 |
| UniProt ID | Q8BHE5 |
| Gene Summary | This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein suppresses osteoblast differentiation, and negatively regulates bone density, by modulating TGF-beta receptor availability to other ligands. Homozygous knockout mice for this gene exhibit increased bone density and volume, while overexpression of this gene in a transgenic mouse causes bone defects resulting in spontaneous rib fractures. This gene encodes distinct protein isoforms that may be similarly proteolytically processed. [provided by RefSeq, Jul 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC216355 | Bmp3 (untagged) - Mouse bone morphogenetic protein 3 (Bmp3), (10ug) |
CNY 6,080.00 |
|
| MR223213 | Bmp3 (Myc-DDK-tagged) - Mouse bone morphogenetic protein 3 (Bmp3) |
CNY 3,656.00 |
|
| MR223213L3 | Lenti ORF clone of Bmp3 (Myc-DDK-tagged) - Mouse bone morphogenetic protein 3 (Bmp3) |
CNY 5,230.00 |
|
| MR223213L4 | Lenti ORF clone of Bmp3 (mGFP-tagged) - Mouse bone morphogenetic protein 3 (Bmp3) |
CNY 5,230.00 |
