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Amer1 (NM_175179) Mouse Tagged ORF Clone
CAT＃: MG222769
- TrueORF®
Amer1 (tGFP-tagged) - Mouse family with sequence similarity 123 member B (Fam123b), (10ug)
ORF Plasmid: DDK
"NM_175179" in other vectors (4)
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CNY 9,220.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 2810002O09Rik; AW492303; Fam123b; Wtx |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG222769 representing NM_175179
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGAGTCAACAGGATGAAGCTGTTCAAACCAAAGGAGCATCAACTTCTAGCGATGCCCAAGACCAGG GAGCTGAAAAGGGAGCCAAGAATAAGACAACTGAGGCAACAGAAGGACCAACATCAGAGCCTCCCTTATC AGGCCCAGGTAGGCTGAAGAAAACTGCCATGAAACTCTTTGGTGGCAAGAAGGGAATCTGTACTCTGCCT AGTTTCTTTGGGGGTGGACGAAGCAAAGGTTCTGGGAAAGTCAGCTCTAAGAAAAGTCTAAACAAGAGTA AGACTCACGATGGTCTAAGTGAAGCGTCTCAGGGACCTGAAGATGTTGTCATTGAAGAAACAGACCTCTC TACACCTTTGTCCAAGTCATCTGCTCAATTTCCTAGCTCCCAGAGTGCCAATGGAGCGTTGGAGATAGGC TCTAAACATAAGACATCTGGGACTGAAGCCATAGAAAAAGCTGGAGTTGAGAAGGTACCCTCTGTGCACA AGCCGAAGAAAAGCCTAAAAAGCTTCTTTAGCAGTATCCGCCGTCACCGGAAGGGCAAGACTTCTGGGGC TGACCAAAGTGTGCCAGGGGCCAAGGAACTTGAGGGGGCAAGAACCAGGTCTCATGAGCATGTGAGTTCA ATCTCTCTGCCCAGCTCTGAGGAGATATTCCGAGACACTAGAAAGGAAAATGCCAAACCCCAGGATGCCC CTGGACCAAAAATGTCTCCAGCACAAGTACATTTTTCACCAACTACTGAGAAGGCAGCTTGTAAAAATCC AGAAAAACTCACAAGGACCTGTGCCTCAGAGTTCATGCAGCCCAAGCCTGTTCTTGAAGGTGGTAGTCTA GAGGAGCCCCACACTTCAGAAACAGAAGGAAAAGTGGTGGCAGGAGAAGTAAACCCACCCAATGGCCCTG TTGGGGATCAGCTAAGCCTCTTGTTTGGGGATGTTACATCGCTAAAAAGCTTTGACTCACTGACAGGTTG TGGTGACATTATAGCAGAACAGGACATGGACAGCATGACAGATAGCATGGCCTCTGGTGGCCAGAGGGCC AACCGAGATGGGACCAAGCGAAGTTCCTGCTTGGTAACCTACCAAGGAGGTGGGGAGGAGATGGCCTTGC CAGATGATGATGACAATGATGATGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA AGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAATTATTAGAGGATGAAGAGGAGGTTAAGGATGGA GAGGAAAATGATGACTTAGAGTATCTGTGGGCAAGTGCCCAGATATACCCAAGATTTAATATGAATCTGG GCTACCATACTGCTATATCTCCCAGCCACCAGGGCTACATGCTTCTTGACCCAGTTCAGTCTTATCCTAA TCTAGGCCTTGGAGAACTTTTGACACCTCAGAGCGACCAGCAAGAGTCTGCTCCCAATAGTGATGAAGGT TATTATGACTCTACCACACCAGGATTTGAGGATGATTCAGGTGAGGCTCTGGGACTTGCCCACAGGGATT GTCTACCCAGGGACAGCTATAGTGGTGATGCGCTATATGAGTTCTATGAGCCAGATGACAGTCTTGAGCA CTCCCCACCTGGGGATGACTGCCTATATGACCTCCGTGGACGCAACTCAGAGATGTTGGATCCTTTCTTG AACTTGGAGCCCTTTTCTTCTCGGCCACCTGGGGCAATGGAAACCGAGGAAGAACGGCTAGTGACCATCC AGAAGCAGTTGCTATACTGGGAACTTCGCCGGGAGCAGCGTGAGGCTCAAGAGGCATGTGCCAGAGAGGC TCATGCCAGAGAAGCTTATGCCCGAGACACCCATACTCGGGAATCTTATGGCAGAAATGTCCGAGCCCGA GAAACTCAGGCCTTAGAGGCTCATTCTCAAGAGGGTCGAGTCCAAGAGACCAAGGTACGGCAGGAGAAGC CTGCCTTGGAGTATCAGATGAGGCCCTTAGGTCCCTCAGTGATGGGTCTGGTGGCAGGGACATCAGGAGG TTCTCAGACTTCTCACCGGGGAACCACTTCAGCTTTCCCTGCTACTTCTAGCAGTGAGCCAGACTGGAGG GATTTCCGTCCTCTGGAGAAGCGTTTTGAGGGGACTTGTTCCAAAAAAGATCAAAGTACTTGTTTAATGC AGCTCTTCCAGAGTGATGCCATGTTTGAGCCAGACATGCAAGAAGCAAATTTTGGAGGCTCACCCAGGAA GGCGTACCCTAGTTACTCACCCCCTGAGGAGCCAGAGGAAGAAGAAGAGGAGAAGGAAGGGAATGCCACT GTGAGTTTCTCACAGGCCCTAGTAGAGTTCACCAGCAATGGAAACCTTTTTACCAGCATGTCCTACAGCT CTGATTCTGACTCATCCTTCACTCAAAACCTCCCTGAGCTGCCCCCCATGGTCACCTTTGATATTGCTGA CGTGGAACGAGATGGAGAAGGCAAGTGTGAAGAGAATCCTGAGTTCAACAATGACGAAGATCTTACAGCC TCCTTGGAAGCTTTTGAGCTGGGTTATTACCACAAACATGCCTTCAACAGCTATCACAGTCGGTTCTACC AAGGCCTGCCCTGGGGAGTTAGCAGCCTCCCTCGATACTTAGGACTCCCCGGAGTCCACCCTCGACCTCC TCCTGCTGCAATGGCACTCAATAGGAGAAGCCGTTCCCTTGACAATGCAGAGTCTCTGGAGCTGGAGCTC TCCAGTTCTCACCTAGCCCAGGGTTACATGGAGTCTGATGAGCTCCAGGCCCACCAGGAAGATTCAGATG AAGAAGGTGAAGAAGAGGAAGGAGAATGGGGTCGGGATAGTCCCTTGTCTCTCTACACTGAACCTCCAGG GGTCTATGACTGGCCTCCCTGGGCTCACTGTCCTCTTCCAGTGGGGCCAGGTCTTGCTTGGATGAGCCCT AATCAGTTGTATGAGCCTTTCAATCAGTCTTCCTATGTGCAGGCAACCTGTTGTGTACCTCCTGTGGCAA TGCCAGTGTCAGTGCCAGGAAGAACACCTGGAGACTCTGTGTCTCAGCTAGCTCGACCTTCGCACCTACC CCTGCCCATGGGCCCTTGCTATAACCTTCAATCACAGGCCTCCCAGAGTGGGAGGGCCAAGCCTCGGGAT GTACTGCTGCCTGTAGATGAGCCCAGTTGCTCTTCCATTTCTGGAGCCAACTCTCAGTCTCAAGCCAAGC CTGTAGGTATCACCCATGGTATCCCTCAGCTGCCCAGGGTGCGGCCTGAACCCTTCCAGCTTCAACCCAA TCACTATCGAGCTTCCAACCTTGACCTGTCAAAGGAGAGGGGTGAGCAAGGTGCCTCTCTCTCTACCAGC TACTCCTCTACTGCCATGAATGGAAACCTAGCCAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG222769 representing NM_175179
Red=Cloning site Green=Tags(s) MESQQDEAVQTKGASTSSDAQDQGAEKGAKNKTTEATEGPTSEPPLSGPGRLKKTAMKLFGGKKGICTLP SFFGGGRSKGSGKVSSKKSLNKSKTHDGLSEASQGPEDVVIEETDLSTPLSKSSAQFPSSQSANGALEIG SKHKTSGTEAIEKAGVEKVPSVHKPKKSLKSFFSSIRRHRKGKTSGADQSVPGAKELEGARTRSHEHVSS ISLPSSEEIFRDTRKENAKPQDAPGPKMSPAQVHFSPTTEKAACKNPEKLTRTCASEFMQPKPVLEGGSL EEPHTSETEGKVVAGEVNPPNGPVGDQLSLLFGDVTSLKSFDSLTGCGDIIAEQDMDSMTDSMASGGQRA NRDGTKRSSCLVTYQGGGEEMALPDDDDNDDEEEEEEEEEEEEEEEEEEEEEEEEEEEELLEDEEEVKDG EENDDLEYLWASAQIYPRFNMNLGYHTAISPSHQGYMLLDPVQSYPNLGLGELLTPQSDQQESAPNSDEG YYDSTTPGFEDDSGEALGLAHRDCLPRDSYSGDALYEFYEPDDSLEHSPPGDDCLYDLRGRNSEMLDPFL NLEPFSSRPPGAMETEEERLVTIQKQLLYWELRREQREAQEACAREAHAREAYARDTHTRESYGRNVRAR ETQALEAHSQEGRVQETKVRQEKPALEYQMRPLGPSVMGLVAGTSGGSQTSHRGTTSAFPATSSSEPDWR DFRPLEKRFEGTCSKKDQSTCLMQLFQSDAMFEPDMQEANFGGSPRKAYPSYSPPEEPEEEEEEKEGNAT VSFSQALVEFTSNGNLFTSMSYSSDSDSSFTQNLPELPPMVTFDIADVERDGEGKCEENPEFNNDEDLTA SLEAFELGYYHKHAFNSYHSRFYQGLPWGVSSLPRYLGLPGVHPRPPPAAMALNRRSRSLDNAESLELEL SSSHLAQGYMESDELQAHQEDSDEEGEEEEGEWGRDSPLSLYTEPPGVYDWPPWAHCPLPVGPGLAWMSP NQLYEPFNQSSYVQATCCVPPVAMPVSVPGRTPGDSVSQLARPSHLPLPMGPCYNLQSQASQSGRAKPRD VLLPVDEPSCSSISGANSQSQAKPVGITHGIPQLPRVRPEPFQLQPNHYRASNLDLSKERGEQGASLSTS YSSTAMNGNLAK TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_175179 |
| ORF Size | 3396 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_175179.4, NP_780388.2 |
| RefSeq Size | 8476 bp |
| RefSeq ORF | 3399 bp |
| Locus ID | 72345 |
| UniProt ID | Q7TS75 |
| Gene Summary | Regulator of the canonical Wnt signaling pathway. Acts by specifically binding phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2), translocating to the cell membrane and interacting with key regulators of the canonical Wnt signaling pathway, such as components of the beta-catenin destruction complex. Acts both as a positive and negative regulator of the Wnt signaling pathway, depending on the context: acts as a positive regulator by promoting LRP6 phosphorylation. Also acts as a negative regulator by acting as a scaffold protein for the beta-catenin destruction complex and promoting stabilization of Axin at the cell membrane. Promotes CTNNB1 ubiquitination and degradation. Involved in kidney development (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223690 | Amer1 (untagged) - Mouse family with sequence similarity 123, member B (Fam123b), (10ug) |
CNY 14,540.00 |
|
| MR222769 | Amer1 (Myc-DDK-tagged) - Mouse family with sequence similarity 123, member B (Fam123b) |
CNY 8,672.00 |
|
| MR222769L3 | Lenti ORF clone of Amer1 (Myc-DDK-tagged) - Mouse family with sequence similarity 123, member B (Fam123b) |
CNY 10,260.00 |
|
| MR222769L4 | Lenti ORF clone of Amer1 (mGFP-tagged) - Mouse family with sequence similarity 123, member B (Fam123b) |
CNY 10,260.00 |
