Scn7a (NM_009135) Mouse Tagged ORF Clone
CAT#: MG222192
- TrueORF®
Scn7a (tGFP-tagged) - Mouse sodium channel voltage-gated type VII alpha (Scn7a), (10ug)
ORF Plasmid: DDK
"NM_009135" in other vectors (2)
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CNY 18,530.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 1110034K09Rik; AI642000; NaG; Nav2; Nav2.3; Nax; Scn6a |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG222192 representing NM_009135
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTGACTTCCCCAGAGCCGAAGGGCCTTGTCCCATTTACAACAGAGTCACTTGAACTTATAGAAAATC ACATTGCTAAAAAATGCAATGAAGACCCCGAAGAAGAAGAAGGTTTAAAACCAAGTCGTAATCTAGAAGC TGGCAAAAGACTTCCAATTCCCTATGGAACCCTCCCTCGAGGAACCGTGTCAGAGCCCTTGGAAGATGTG GATCCATACTACTATGTTAAGAGAAATACTTTCATGGTCTTAAACAGAAGCAGAGTCATCTTCAGGTTCA ATGCGGTTTCCATCTTCTGCACATTGTCTCCTCTAAACTCCCTCAGAAGAGCAGCTATCAAGGCTTTGGT GCATCCCCTTTTTCGCCTGCTGATTTTAATCAGCGTTCTCACTGACAGCATACTTATGTGCATGAGTAAT CTACCAGAATGGATATTGGCAATAGAGAATACTTTGCTTGGGATTTACGCATTTGAAATACTTGTAAAAG TCATTGCAAGAGGTATCTGGGCAGGTTCATTTTCCTTCCTTGGGGATCTTTGGAACTGGCTTGATTTCAG TGTAACTTTGTTCGAGCTAATCACAAGGTTTTCACCTCTAAGCTCCTTTTTAATGCTTAAAACTATCAGA ACTTTCCGAATTTTGAAGATTATCCCTTTGAACCACGGCCTGCAGTCTATTGTGATGACACTGGCCCAGT GTTTGAAGAAACTATTTGGTGCCATTGCCCTAGCTCTGTTTTTTCTGGCTGTGTTTTCACTACTTGGAAT GGGCCTCTTCATGGGCAACCTGAAGCACAAATGTCTGCGGTGGCCAGAAGAAAATGAAAATGAAACGCTG CACAACAGAACTGGAAGCCTTAACTATAGTCCAGAAAGAATAAACTTCTACTACATGGAAGGAGCGAAAT ATGCTCTCCTTTGCGGCAACAGGACAGATGCTGGCCAGTGTCCGGAAGGTTATGTGTGTGTAAAAGAAGG CACAAATCCTGACAATGGCTTCACAAGTTTTGACAACTTTGGCTGGTCCCTTCTTGCTATGTTTCGATTG ATGACACAGGATTACCCTGAATTACTTTATCACCAGATCCTTTATGCTTCAGGAAAGGTCTACATGATAT TTTTTGTTATGATCAGTTTCTGGTTTGCCTTCTATTTGACAAGTTTGTTCTTGGGCATACTCACTATGAC CTATGAAAAGGAAAAGCAGAGAGCCTGTGAGGAATCTGGAGGCCTTGATCCCAAATGTCAACAGACAGTG AAAGAACTCGACGAAGAAAATGACGCAGCTGAGATGGAAACTACACAAATAGAAATGAAGAAAAGATCCC CAACTTCTATAAACACCACACTGGATATACTGGAAGACACTACCCTCGGACACAGAGAAGAACCAGAAAC ATCCAGGAAGAAATGCCCAATATGTTGGCATAAGTTTATTAAAACCTGCTTCATCTGGAAATGCTCTCCC TGTTGGGTAAAACTGAACGAGTTTGCTGATAGAGTTATAACACACCCATTGGCTGATCTTTTTCTTGTCA TCTGCATCGTTTTAAACATATGCTTCCTCGCCCTAGAACATTTTCCAATGAGCGAGGAGCTCAGGTCTCT CCTTCACGTTGGAAATTTGGTTTTTATTGGAATTTACACAATAGAAATGATTTTGAAGATAATCGCTATG CATCCATATGGGTATTTTCAAATAAGCTGGAATATTTTTGACAGTATACTTGTGGTTTTGGAGTTAACAG AAATTTTACTAGCAGATGTTGAAGGACTAGCTGTTTTAATAACAGTCCCATTGATATTTATAAAACTGGG GAAGTACGGACCACCATTTAAGAGTTTGATGCGCATCCTTGGTAGCTCATTGATGGCCCTGAAAGATTTG GTCCTGTTGCTCTGCATATTCGTTTACTTCTCTGCTGTGTTCGGCATGAAGCTGTTTGGTCGAAGTTACA AGGATTGTGTCTGCCACATAAAGGAAGACTGCCAACCCCAACGCTGGCACATGAGTGACTTCCTTCATGC CTACATGACCGTGTTCCGAATCCTCTGTGGAGAGTGGATAGAGACATTATGGGAGTGTATGGAGGTTGCA GGCCAGGCCTGGTGTATTCCTTTTTACATGATGGTCATTTTAATTGGAAACTTATTGATACTTTACCTCT TTGTGACATTGGTGAGCTCTTTCAGTTACTATGATGCTACATCGGAAGTGAACAAAGAAGCCAAAAACCT TCAGCTTGCCATGGCAAGGATAAAGTCGGGAATAAACTCCATGCTTCTTAAATTAATGTGCACAGAAAGA AGTGTTCCTACAGAAGCAACAGACCAAATATGTGATCCAAGTGTTAAAGAGAATATTTCTGGCCATACTC TTTCTGAACTGAGCAACACCCAAACTTTCCTCAGATATAAGGACCAGAGCAGCAGCACTGAGAAAACTCC AGTGACTGAATCTGAGAGTCAATCTCTGATTGCTAGTCCCAGTGCCTCTGAAACTGTGCCGATTGCTTCA GGAGAATCTGATATAGAAAATCTGGATAACAAGGAGACTCGGAGCAAGTCTGGGAATGGAGGCAGTAAAG AGAAAATGAAGCAGTCTAGCTCATCTGAGTGCAGCACAGTTGATATCGCTATTTCTGAAGAAGAAGAAAT GGTCTATGAACATGAAAAGTCAAAGCTTCATAAAAATGGTTATGAACGCAAATCTTCAACTGGTCAAATC AGTAGAGAATCTAGGAATGGAAAGATTTGGAAAAACATCAGGAAAACTTGCTGCAAGATAGTAGAGAACA GCTGGTTTGAGTGTTTCATTGGCCTGGTCACTCTGCTCTGCACAGGCACTCTGGCTCTTGAAGACATATA TATTGATCAGAGAAAAACCACTAAAATCTTACTGGAATATGCGGACATGATATTTGCATACATCTTCATT CTGGAGATGCTTCTCAAGTGGGTGGCTTATGGCTTTAAAGCCTTTTTCTCCAACAACTGGTACAAACTGG ACTTTATGGTTGTTATCGTGTTTTGTCTTAGCTTAATAGGCAAAACTCGAGAAGATCTGAACCCTCTGAC ATCAATAAAGTTCCTTCGAGCACTAAGAGTTCTGTCGCAGTTTGAAAGAATGAAGGTGGTCCTGAGAGCT TTGATAAAAACAACCTTACCCACTGTGAGCGTGTTTCTAGTCTGCCTAATGATCTGGCTGCTTTTCAGTG TTATTGGAGTGCAGTTATTTGCTGGCAAGTTCTATGAATGCATTGACCCAACAAAGGGAGAAAGATTCCC TGTATTTGAAGTTATGAATAAAAGTCAGTGTGAAAAACTGTTATTCAATGAATCAATGCCGTGGGAGAAT GCAAAACTGAACTTTGATAATGTTGGAAATGGTTTTCTTTCTTTACTCCAAGTGGCAACATTTAATGGAT GGATCAGTATTATGAATTCAGCAATTGATTCTGTTGGTGTAAACATGCAGCCCAGCTTTGAGTACAACCT CTACATGTATAGTTACTTTATCATCTTTGTTATCTTTGGATTATTTCTTCCTCTCTGTATGCTGATTGGT GTTATTATTCGCAATTTCAACAAGCAGAAAATTAAGCAGGGAGGATCAAACATCTTTATAACAGTAAAAC AGAAAAAACAGTACCGGGCCCTGAAGAAGCTCTTGTATGCAGACGTCCAGAAACCAACACCCCGCCCCAG AAACAAATTCCAAGGCTTCCTTTTTGACCTAGTAACACACCGTGTCTTTAATGTTATCATCATACTTCTT ATCTGTTTCCAAGCAACAACCATTATGATACAAAAGGATGAGCAGAGTCCACAAATGGAAACTGCCATCT TCTGGATGAACTCCATTTTTGTCATGCTGTTCACTCTGGAATGCATACTGAAGCTCACTGCCTTCCGTTG CCACTACTTCACCAGTGCATGGAATGTTCATGACTTTATGGTGGTCATTTTCTCCATTACAGGGCTGCTG CTACCCTTGACAATAGGACAATACTTTGTGCCTCCTTCCCTGGTGCAGCTGATTCTTCTCTCTCGAGTCA TCCACATCCTGCGTCCTGGGAAAGGACCGAAGGTGTTCCATGACCTGATGCTTCCCTTGATTCTGGCGCT CCCAGCATTGCTGAACATTAGTCTTCTCATCTTCCTGGTCATGTTCATCTACGCCATCTTTGGAATGTAC AACTTTGCCTATGTAAAGAAAGAAGCCGGAATTAATGATGTGTCCAACTTTGAGACCTTTGGAAGCAGTA TGCTCTGTCTCTTCCAAGTTACAACGTTTTCTGGTTGGGACGGGATGCTGGATGCAATTTTCAACAGTCA GTGGTCTGACTGCGATCCTGATAAAATTAATCCAGGGACTCAGGTCAAGGGAGATTGTGGGAGCCCTTCT GTTGGGATTTCTTATTTTGTCAGTTACATCCTCATATCATGGTTGATCATTGTTAACATGTACATTGTGT TGATCATGGAGTTCTTAAGTATTCCTTCTCAGAAAAAAAGCAGGACCTTGAGTGAAGATGACTTTAGGAG ATTCTTCCGGGTGTGGAACAGGTTTGACCCTGATAGGACCCAGTACATAGACTCTAGCAAGCTTTCTGAT TTTGCAGCTGCTCTGGATCCTCCTCTTTTCATGGCAAAACCAAACAAGGGCCAGCTTGTGGCCATGGATC TCCCCATGGCTGCGGGAGACAGAATCCACTGCCTCGACATTTTACTTGCCTTTACGAAAAGAGTGATGGG AAAGGATGAGAGGGTGGAGAAAATCCTTTCAGAGATAGAATCCGGGTTCATGTTAGCGAACCCTTTCAAA ATCACTTATGAGCCGATTACAACTACTTTGAAACGCAAACAAGAGGCAGTTTCAGCAACCATCATCCAGC GTGCATATAAAAGCTACCGCTTAAGGCAAAATGACAAGAATGTATCAGATACTCCTGCTATAGATGACCG CAGAGATGATCTTACTTCTAAAGGTGCTCACTCTGGCAAAATCGAGGAGAAGGCATCTATTCAGACCCAG ATT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG222192 representing NM_009135
Red=Cloning site Green=Tags(s) MLTSPEPKGLVPFTTESLELIENHIAKKCNEDPEEEEGLKPSRNLEAGKRLPIPYGTLPRGTVSEPLEDV DPYYYVKRNTFMVLNRSRVIFRFNAVSIFCTLSPLNSLRRAAIKALVHPLFRLLILISVLTDSILMCMSN LPEWILAIENTLLGIYAFEILVKVIARGIWAGSFSFLGDLWNWLDFSVTLFELITRFSPLSSFLMLKTIR TFRILKIIPLNHGLQSIVMTLAQCLKKLFGAIALALFFLAVFSLLGMGLFMGNLKHKCLRWPEENENETL HNRTGSLNYSPERINFYYMEGAKYALLCGNRTDAGQCPEGYVCVKEGTNPDNGFTSFDNFGWSLLAMFRL MTQDYPELLYHQILYASGKVYMIFFVMISFWFAFYLTSLFLGILTMTYEKEKQRACEESGGLDPKCQQTV KELDEENDAAEMETTQIEMKKRSPTSINTTLDILEDTTLGHREEPETSRKKCPICWHKFIKTCFIWKCSP CWVKLNEFADRVITHPLADLFLVICIVLNICFLALEHFPMSEELRSLLHVGNLVFIGIYTIEMILKIIAM HPYGYFQISWNIFDSILVVLELTEILLADVEGLAVLITVPLIFIKLGKYGPPFKSLMRILGSSLMALKDL VLLLCIFVYFSAVFGMKLFGRSYKDCVCHIKEDCQPQRWHMSDFLHAYMTVFRILCGEWIETLWECMEVA GQAWCIPFYMMVILIGNLLILYLFVTLVSSFSYYDATSEVNKEAKNLQLAMARIKSGINSMLLKLMCTER SVPTEATDQICDPSVKENISGHTLSELSNTQTFLRYKDQSSSTEKTPVTESESQSLIASPSASETVPIAS GESDIENLDNKETRSKSGNGGSKEKMKQSSSSECSTVDIAISEEEEMVYEHEKSKLHKNGYERKSSTGQI SRESRNGKIWKNIRKTCCKIVENSWFECFIGLVTLLCTGTLALEDIYIDQRKTTKILLEYADMIFAYIFI LEMLLKWVAYGFKAFFSNNWYKLDFMVVIVFCLSLIGKTREDLNPLTSIKFLRALRVLSQFERMKVVLRA LIKTTLPTVSVFLVCLMIWLLFSVIGVQLFAGKFYECIDPTKGERFPVFEVMNKSQCEKLLFNESMPWEN AKLNFDNVGNGFLSLLQVATFNGWISIMNSAIDSVGVNMQPSFEYNLYMYSYFIIFVIFGLFLPLCMLIG VIIRNFNKQKIKQGGSNIFITVKQKKQYRALKKLLYADVQKPTPRPRNKFQGFLFDLVTHRVFNVIIILL ICFQATTIMIQKDEQSPQMETAIFWMNSIFVMLFTLECILKLTAFRCHYFTSAWNVHDFMVVIFSITGLL LPLTIGQYFVPPSLVQLILLSRVIHILRPGKGPKVFHDLMLPLILALPALLNISLLIFLVMFIYAIFGMY NFAYVKKEAGINDVSNFETFGSSMLCLFQVTTFSGWDGMLDAIFNSQWSDCDPDKINPGTQVKGDCGSPS VGISYFVSYILISWLIIVNMYIVLIMEFLSIPSQKKSRTLSEDDFRRFFRVWNRFDPDRTQYIDSSKLSD FAAALDPPLFMAKPNKGQLVAMDLPMAAGDRIHCLDILLAFTKRVMGKDERVEKILSEIESGFMLANPFK ITYEPITTTLKRKQEAVSATIIQRAYKSYRLRQNDKNVSDTPAIDDRRDDLTSKGAHSGKIEEKASIQTQ I TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_009135 |
ORF Size | 5043 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_009135.2, NP_033161.2 |
RefSeq Size | 7333 bp |
RefSeq ORF | 5046 bp |
Locus ID | 20272 |
Documents
Product Manuals |
FAQs |
SDS |