Scn7a (NM_009135) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1110034K09Rik; AI642000; NaG; Nav2; Nav2.3; Nax; Scn6a
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224815 representing NM_009135 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTGACTTCCCCAGAGCCGAAGGGCCTTGTCCCATTTACAACAGAGTCACTTGAACTTATAGAAAATC ACATTGCTAAAAAATGCAATGAAGACCCCGAAGAAGAAGAAGGTTTAAAACCAAGTCGTAATCTAGAAGC TGGCAAAAGACTTCCAATTCCCTATGGAACCCTCCCTCGAGGAACCGTGTCAGAGCCCTTGGAAGATGTG GATCCATACTACTATGTTAAGAGAAATACTTTCATGGTCTTAAACAGAAGCAGAGTCATCTTCAGGTTCA ATGCGGTTTCCATCTTCTGCACATTGTCTCCTCTAAACTCCCTCAGAAGAGCAGCTATCAAGGCTTTGGT GCATCCCCTTTTTCGCCTGCTGATTTTAATCAGCGTTCTCACTGACAGCATACTTATGTGCATGAGTAAT CTACCAGAATGGATATTGGCAATAGAGAATACTTTGCTTGGGATTTACGCATTTGAAATACTTGTAAAAG TCATTGCAAGAGGTATCTGGGCAGGTTCATTTTCCTTCCTTGGGGATCTTTGGAACTGGCTTGATTTCAG TGTAACTTTGTTCGAGCTAATCACAAGGTTTTCACCTCTAAGCTCCTTTTTAATGCTTAAAACTATCAGA ACTTTCCGAATTTTGAAGATTATCCCTTTGAACCACGGCCTGCAGTCTATTGTGATGACACTGGCCCAGT GTTTGAAGAAACTATTTGGTGCCATTGCCCTAGCTCTGTTTTTTCTGGCTGTGTTTTCACTACTTGGAAT GGGCCTCTTCATGGGCAACCTGAAGCACAAATGTCTGCGGTGGCCAGAAGAAAATGAAAATGAAACGCTG CACAACAGAACTGGAAGCCTTAACTATAGTCCAGAAAGAATAAACTTCTACTACATGGAAGGAGCGAAAT ATGCTCTCCTTTGCGGCAACAGGACAGATGCTGGCCAGTGTCCGGAAGGTTATGTGTGTGTAAAAGAAGG CACAAATCCTGACAATGGCTTCACAAGTTTTGACAACTTTGGCTGGTCCCTTCTTGCTATGTTTCGATTG ATGACACAGGATTACCCTGAATTACTTTATCACCAGATCCTTTATGCTTCAGGAAAGGTCTACATGATAT TTTTTGTTATGATCAGTTTCTGGTTTGCCTTCTATTTGACAAGTTTGTTCTTGGGCATACTCACTATGAC CTATGAAAAGGAAAAGCAGAGAGCCTGTGAGGAATCTGGAGGCCTTGATCCCAAATGTCAACAGACAGTG AAAGAACTCGACGAAGAAAATGACGCAGCTGAGATGGAAACTACACAAATAGAAATGAAGAAAAGATCCC CAACTTCTATAAACACCACACTGGATATACTGGAAGACACTACCCTCGGACACAGAGAAGAACCAGAAAC ATCCAGGAAGAAATGCCCAATATGTTGGCATAAGTTTATTAAAACCTGCTTCATCTGGAAATGCTCTCCC TGTTGGGTAAAACTGAACGAGTTTGCTGATAGAGTTATAACACACCCATTGGCTGATCTTTTTCTTGTCA TCTGCATCGTTTTAAACATATGCTTCCTCGCCCTAGAACATTTTCCAATGAGCGAGGAGCTCAGGTCTCT CCTTCACGTTGGAAATTTGGTTTTTATTGGAATTTACACAATAGAAATGATTTTGAAGATAATCGCTATG CATCCATATGGGTATTTTCAAATAAGCTGGAATATTTTTGACAGTATACTTGTGGTTTTGGAGTTAACAG AAATTTTACTAGCAGATGTTGAAGGACTAGCTGTTTTAATAACAGTCCCATTGATATTTATAAAACTGGG GAAGTACGGACCACCATTTAAGAGTTTGATGCGCATCCTTGGTAGCTCATTGATGGCCCTGAAAGATTTG GTCCTGTTGCTCTGCATATTCGTTTACTTCTCTGCTGTGTTCGGCATGAAGCTGTTTGGTCGAAGTTACA AGGATTGTGTCTGCCACATAAAGGAAGACTGCCAACCCCAACGCTGGCACATGAGTGACTTCCTTCATGC CTACATGACCGTGTTCCGAATCCTCTGTGGAGAGTGGATAGAGACATTATGGGAGTGTATGGAGGTTGCA GGCCAGGCCTGGTGTATTCCTTTTTACATGATGGTCATTTTAATTGGAAACTTATTGATACTTTACCTCT TTGTGACATTGGTGAGCTCTTTCAGTTACTATGATGCTACATCGGAAGTGAACAAAGAAGCCAAAAACCT TCAGCTTGCCATGGCAAGGATAAAGTCGGGAATAAACTCCATGCTTCTTAAATTAATGTGCACAGAAAGA AGTGTTCCTACAGAAGCAACAGACCAAATATGTGATCCAAGTGTTAAAGAGAATATTTCTGGCCATACTC TTTCTGAACTGAGCAACACCCAAACTTTCCTCAGATATAAGGACCAGAGCAGCAGCACTGAGAAAACTCC AGTGACTGAATCTGAGAGTCAATCTCTGATTGCTAGTCCCAGTGCCTCTGAAACTGTGCCGATTGCTTCA GGAGAATCTGATATAGAAAATCTGGATAACAAGGAGACTCGGAGCAAGTCTGGGAATGGAGGCAGTAAAG AGAAAATGAAGCAGTCTAGCTCATCTGAGTGCAGCACAGTTGATATCGCTATTTCTGAAGAAGAAGAAAT GGTCTATGAACATGAAAAGTCAAAGCTTCATAAAAATGGTTATGAACGCAAATCTTCAACTGGTCAAATC AGTAGAGAATCTAGGAATGGAAAGATTTGGAAAAACATCAGGAAAACTTGCTGCAAGATAGTAGAGAACA GCTGGTTTGAGTGTTTCATTGGCCTGGTCACTCTGCTCTGCACAGGCACTCTGGCTCTTGAAGACATATA TATTGATCAGAGAAAAACCACTAAAATCTTACTGGAATATGCGGACATGATATTTGCATACATCTTCATT CTGGAGATGCTTCTCAAGTGGGTGGCTTATGGCTTTAAAGCCTTTTTCTCCAACAACTGGTACAAACTGG ACTTTATGGTTGTTATCGTGTTTTGTCTTAGCTTAATAGGCAAAACTCGAGAAGATCTGAACCCTCTGAC ATCAATAAAGTTCCTTCGAGCACTAAGAGTTCTGTCGCAGTTTGAAAGAATGAAGGTGGTCCTGAGAGCT TTGATAAAAACAACCTTACCCACTGTGAGCGTGTTTCTAGTCTGCCTAATGATCTGGCTGCTTTTCAGTG TTATTGGAGTGCAGTTATTTGCTGGCAAGTTCTATGAATGCATTGACCCAACAAAGGGAGAAAGATTCCC TGTATTTGAAGTTATGAATAAAAGTCAGTGTGAAAAACTGTTATTCAATGAATCAATGCCGTGGGAGAAT GCAAAACTGAACTTTGATAATGTTGGAAATGGTTTTCTTTCTTTACTCCAAGTGGCAACATTTAATGGAT GGATCAGTATTATGAATTCAGCAATTGATTCTGTTGGTGTAAACATGCAGCCCAGCTTTGAGTACAACCT CTACATGTATAGTTACTTTATCATCTTTGTTATCTTTGGATTATTTCTTCCTCTCTGTATGCTGATTGGT GTTATTATTCGCAATTTCAACAAGCAGAAAATTAAGCAGGGAGGATCAAACATCTTTATAACAGTAAAAC AGAAAAAACAGTACCGGGCCCTGAAGAAGCTCTTGTATGCAGACGTCCAGAAACCAACACCCCGCCCCAG AAACAAATTCCAAGGCTTCCTTTTTGACCTAGTAACACACCGTGTCTTTAATGTTATCATCATACTTCTT ATCTGTTTCCAAGCAACAACCATTATGATACAAAAGGATGAGCAGAGTCCACAAATGGAAACTGCCATCT TCTGGATGAACTCCATTTTTGTCATGCTGTTCACTCTGGAATGCATACTGAAGCTCACTGCCTTCCGTTG CCACTACTTCACCAGTGCATGGAATGTTCATGACTTTATGGTGGTCATTTTCTCCATTACAGGGCTGCTG CTACCCTTGACAATAGGACAATACTTTGTGCCTCCTTCCCTGGTGCAGCTGATTCTTCTCTCTCGAGTCA TCCACATCCTGCGTCCTGGGAAAGGACCGAAGGTGTTCCATGACCTGATGCTTCCCTTGATTCTGGCGCT CCCAGCATTGCTGAACATTAGTCTTCTCATCTTCCTGGTCATGTTCATCTACGCCATCTTTGGAATGTAC AACTTTGCCTATGTAAAGAAAGAAGCCGGAATTAATGATGTGTCCAACTTTGAGACCTTTGGAAGCAGTA TGCTCTGTCTCTTCCAAGTTACAACGTTTTCTGGTTGGGACGGGATGCTGGATGCAATTTTCAACAGTCA GTGGTCTGACTGCGATCCTGATAAAATTAATCCAGGGACTCAGGTCAAGGGAGATTGTGGGAGCCCTTCT GTTGGGATTTCTTATTTTGTCAGTTACATCCTCATATCATGGTTGATCATTGTTAACATGTACATTGTGT TGATCATGGAGTTCTTAAGTATTCCTTCTCAGAAAAAAAGCAGGACCTTGAGTGAAGATGACTTTAGGAG ATTCTTCCGGGTGTGGAACAGGTTTGACCCTGATAGGACCCAGTACATAGACTCTAGCAAGCTTTCTGAT TTTGCAGCTGCTCTGGATCCTCCTCTTTTCATGGCAAAACCAAACAAGGGCCAGCTTGTGGCCATGGATC TCCCCATGGCTGCGGGAGACAGAATCCACTGCCTCGACATTTTACTTGCCTTTACGAAAAGAGTGATGGG AAAGGATGAGAGGGTGGAGAAAATCCTTTCAGAGATAGAATCCGGGTTCATGTTAGCGAACCCTTTCAAA ATCACTTATGAGCCGATTACAACTACTTTGAAACGCAAACAAGAGGCAGTTTCAGCAACCATCATCCAGC GTGCATATAAAAGCTACCGCTTAAGGCAAAATGACAAGAATGTATCAGATACTCCTGCTATAGATGACCG CAGAGATGATCTTACTTCTAAAGGTGCTCACTCTGGCAAAATCGAGGAGAAGGCATCTATTCAGACCCAG ATTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_009135
Insert Size	5046 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_009135.2, NP_033161.2
RefSeq Size	7333 bp
RefSeq ORF	5046 bp
Locus ID	20272

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