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Myl1 (NM_021285) Mouse Tagged ORF Clone
CAT＃: MG221651
- TrueORF®
Myl1 (tGFP-tagged) - Mouse myosin light polypeptide 1 (Myl1) transcript variant 1f, (10ug)
ORF Plasmid: DDK
"NM_021285" in other vectors (4)
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CNY 2,850.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AI325107; D12Mgi4; MLC1; MLC1f; MLC3; MLC3f; Myl; Mylf |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG221651 representing NM_021285
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCACCAAAGAAAGACGTGAAGAAGCCCGCTGCTGCGCCTGCCCCAGCCCCGGCCCCGGCCCCTGCCC CAGCCAAACCTAAGGAAGAAAAAATCGATCTGTCTGCTATTAAGATCGAGTTCTCTAAGGAGCAACAGGA GGACTTCAAGGAGGCATTTCTCCTGTTTGACAGAACAGGTGAATGCAAGATCACCTTAAGTCAGGTGGGA GACGTCCTCCGGGCTCTGGGCACCAATCCCACCAATGCAGAGGTCAAGAAGGTTCTTGGGAACCCCAGCA ATGAAGAGATGAATGCTAAGAAAATCGAGTTTGAACAGTTTCTGCCCATGATGCAAGCTATCTCCAACAA CAAGGACCAGGGAGGTTATGAAGATTTCGTTGAGGGTCTGCGTGTCTTCGACAAGGAGGGCAATGGCACC GTCATGGGTGCTGAACTCCGCCATGTCCTCGCCACTCTGGGAGAGAAGATGAAGGAGGAAGAGGTAGAAG CGTTGCTGGCAGGCCAGGAGGACTCCAATGGCTGCATCAACTATGAAGCTTTTGTCAAACACATCATGTC TGTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG221651 representing NM_021285
Red=Cloning site Green=Tags(s) MAPKKDVKKPAAAPAPAPAPAPAPAKPKEEKIDLSAIKIEFSKEQQEDFKEAFLLFDRTGECKITLSQVG DVLRALGTNPTNAEVKKVLGNPSNEEMNAKKIEFEQFLPMMQAISNNKDQGGYEDFVEGLRVFDKEGNGT VMGAELRHVLATLGEKMKEEEVEALLAGQEDSNGCINYEAFVKHIMSV TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_021285 |
| ORF Size | 564 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_021285.3, NP_067260.1 |
| RefSeq Size | 1003 bp |
| RefSeq ORF | 567 bp |
| Locus ID | 17901 |
| UniProt ID | P05977 |
| Gene Summary | Myosin is a hexameric ATPase cellular motor protein. It is composed of two heavy chains, two non-phosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. This gene encodes a myosin alkali light chain expressed in fast skeletal muscle. Multiple transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC209004 | Myl1 (untagged) - Mouse myosin, light polypeptide 1 (Myl1), transcript variant 1f, (10ug) |
CNY 3,990.00 |
|
| MR221651 | Myl1 (Myc-DDK-tagged) - Mouse myosin, light polypeptide 1 (Myl1), transcript variant 1f |
CNY 2,400.00 |
|
| MR221651L3 | Lenti ORF clone of Myl1 (Myc-DDK-tagged) - Mouse myosin, light polypeptide 1 (Myl1), transcript variant 1f |
CNY 4,750.00 |
|
| MR221651L4 | Lenti ORF clone of Myl1 (mGFP-tagged) - Mouse myosin, light polypeptide 1 (Myl1), transcript variant 1f |
CNY 4,750.00 |
