United States
Germany
Japan
United Kingdom
China
Ube2o (NM_173755) Mouse Tagged ORF Clone
CAT＃: MG220692
- TrueORF®
Ube2o (tGFP-tagged) - Mouse ubiquitin-conjugating enzyme E2O (Ube2o), (10ug)
ORF Plasmid: DDK
"NM_173755" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 12,540.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 9630022H21; B230113M03Rik; E2-230K; mKIAA1734 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG220692 representing NM_173755
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGATCCCGCAGCGCCCGCACCCGCCCAGGCCCAGGCCGCCGCCGCCCCGACCCCGGCCGCAGCCC CCGCCGCCGCCGCCCCGCCGCCCGCGCCCGCTACGGACTCGGCCTCAGGGCCGTCCTCCGACTCCGGCCC CGAAGCAGGCTCACAGCGCCTGCTGTTCTCCCACGACCTGGTGTCCGGCCGTTACCGCGGCTCGGTGCAC TTCGGGCTGGTACGCCTCATCCACGGCGAGGACTCGGACTCGGAGGGCGACGATGACGGCCGCGGCAGCT CGGGCTGCTCCGAGGCCGGGGGCGCGGGCCACGAGGAGGGCCGGGCCAGCCCTCTGCGGCGCGGCTACGT GCGCGTCCAGTGGTACCCGGAGGGCGTCAAGCAGCATGTGAAGGAGACCAAGCTGAAACTGGAGGACCGC TCTGTGGTGCCCCGGGATGTGGTTCGACACATGCGCTCAACTGACAGCCAGTGTGGCACGGTGATAGATG TCAACATTGACTGTGCCGTCAAGCTCATCGGCACCAACTGCATCATCTACCCTGTCAACAGCAAGGACCT CCAGCACATCTGGCCCTTCATGTATGGAGACTATATCGCCTATGACTGCTGGCTGGGGAAGGTCTATGAC TTGAAGAACCAGATCATCCTCAAGTTGTCTAATGGTGCCAGGTGCTCCATGAACACAGAAGATGGTGCCA AACTCTACGATGTCTGCCCTCACGTCAGCGACTCGGGTCTCTTCTTTGATGACTCTTATGGCTTCTACCC AGGCCAAGTCCTTATTGGCCCAGCCAAGATCTTCTCCAGTGTCCAGTGGCTCTCTGGGGTCAAGCCTGTA CTCAGCACCAAGAGCAAGTTCCGTGTGGTGGTGGAGGAGGTGCAGGTTGTAGAACTGAAAGTCACGTGGA TTACCAAGAGCTTCTGCCCCGGCGGCACGGACAGCGTTAGCCCCCCACCTTCCATCATCACTCAGGAAAA CCTAGGCAGGGTGAAGCGTCTCGGATGCTTTGACCATGCTCAGCGGCAGCTTGGGGAACGCTGTCTGTAT GTCTTCCCAGCCAAGGTAGAGCCGGCCAAGATTGCCTGGGAATGTCCAGAAAAAAACTGCGCCCAGGGGG AGGGCTCTATGGCCAAGAAGGTGAAGCGTCTGTTGAAGAAGCAAGTTGTGAGAATCATGTCCTGCACCCC AGATACCCAGTGTCCTAGGGACCATTCCATGGAAGACCCAGACAAGAAAGGGGAAGCCAGAGCCGGGAGT GAAATAGGGTCTGCTAGCCCGGAGGAGCAACCTGATGGATCAGCCAGCCCCGTGGAGATGCAGGATGAGG GGTCGGAGGAGCTTCAGGAGACATGCGAGCCACTGCCCCCCTTCCTCTTGAAGGAGGGTGGAGATGACGG GCTGCACTCGGCAGAGCAGGACGCAGATGATGAGGCGGCTGACGATACAGATGACACCAGCTCTGTGACC TCCTCCGCCAGCTCTACTACCTCCTCCCAGAGTGGCAGTGGCACTGGTCGTAAAAAGAGCATTCCCTTGT CCATCAAAAACTTGAAGCGAAAACACAAGAGGAAGAAGAATAAGGTCACCCGTGACTTCAAGCCTGGAGA CAGGGTGGCAGTGGAGGTTGTGACCACGATGACCTCAGCTGATGTGATGTGGCAAGATGGCTCTGTGGAA TGCAACATCCGCTCCAACGACCTTTTCCCTGTGCACCACCTGGACAACAATGAGTTCTGCCCTGGGGACT TCGTGGTGGACAAGCGAGTCCAGAGCTGCCCAGACCCTGCTGTCTATGGTGTGGTGCAGTCTGGGGATCA TGTTGGCCGTACCTGCATGGTGAAGTGGTTCAAGCTGCGACCAAGTGGGGATGACGTGGAGCTCATTGGA GAAGAGGAGGACGTGAGCGTCTATGACATTGCTGACCACCCTGACTTTCGCTTCCGCACAACTGACATTG TCATCCGCATTGGTAACACGGAGGATGGGGCTCTACCCAAGGAGGATGAGCCCTCAGTGGGCCAGGTGGC CCGAGTGGATGTCAGCAGCAAAGTGGAAGTGGTGTGGGCTGACAACTCGAAAACCATCATCCTGCCACAG CACTTATACAACATCGAGTCTGAGATTGAGGAGTCTGACTACGACTCCGTGGAAGGCAGCTCCAGTGGGG CCTCCTCGGATGAGTGGGAGGACGACAGCGACAGCTGGGAGACCGACAACGGGCTCGTGGACGATGAGCA CCCCAAGATAGAGGAGCTGGCCGCCATCTTGCCTGCCGAGCAGCCCACTGCCCCCGAGGAGGACAAGGGC GTGGTCATCAGTGAGGAGGCGGCCACGGCTGCCATCCAGGGCGCCGTGGCCATGGCTGCCCCCGTGGCAG GGCTGATGGAGAAAGCTGGGAAGGACGGGCCTCCCAAGAGTTTCCGGGAGCTGAAGGAGGCCATCAAGAT CCTGGAGAGCCTCAAGAACATGACTGTGGAGCAGCTGCTCACAGGCTCGCCCACCTCGCCCACCGTGGAG CCTGAGAAGCCAACCCGAGAGAAGAAGTTTTTGGACGATATCAAGAAGCTACAGGAGAACCTCAAGAAGA CCCTGGACAATGTGGCCATTGCTGAGGAGGAGAAGATGGAGGCAGTACCCGACACAGAGCGCAAGGAGGA GAAGCCTGAGGTGCAGTCCCCAGTGAAAGCCGAGTGGCCCAGTGAGACACCTGTGCTCTGTCAGCAGTGC GGTGGCAGGCCTGGCGTCACCTTCACCAGCGCCAAGGGCGAGGTCTTCTCTGTGCTGGAGTTTGCACCCT CAAACCACTCTTTCAAGAAGATTGAGTTCCAGCCCCCGGAAGCCAAGAAGTTCTTCAGCACAGTGCGGAA GGAGATGGCTCTGCTGGCCACCTCGCTGCCCGATGGCATCATGGTCAAGACTTTTGAGGACAGAATGGAC CTCTTCTCTGCACTGATCAAGGGCCCCACTCGGACTCCCTATGAAGACGGCCTCTACCTATTTGACATCC AGCTTCCCAACATCTACCCTGCGGTGCCCCCCCACTTTTGCTACCTCTCCCAGTGCAGTGGCCGCCTGAA CCCCAACCTGTATGACAATGGGAAAGTGTGTGTCAGCCTCCTAGGTACCTGGATTGGAAAGGGGACAGAG AGGTGGACAAGCAAGTCTAGCCTTCTCCAGGTGCTCATCTCCATCCAAGGTCTGATCCTGGTGAATGAAC CATACTACAATGAAGCTGGCTTTGACAGTGACCGGGGCCTGCAGGAAGGCTATGAGAACAGTCGCTGCTA CAACGAGATGGCATTAATCCGTGTGGTGCAGTCCATGACCCAGTTGGTGCGACGACCTCCTGAGGTCTTC GAACAGGAGATTCGGCAGCACTTCAGTGTGGGTGGCTGGCGGCTGGTGAACCGAATTGAGTCCTGGCTGG AAACCCATGCCATGCAGGAGAGGGCCCAGGTGATGCCCAATGGGGCCCTTAAAGACAGCAGCTCTCTGGA GCCCATGGCTGCAGCTGAACTCTCAGACTCCGGCCGTGAAGAGCCTGAAGATGTTGGGATGGCCCCTGGG GAGGCGTCCCAGGGCTCAGACTCAGAGGGTGGAGCCCAGGGCCCTGCCTCAGCCAGCAGAGACCACACAG AGCAGACTGAGACAGCCCCTGATGCATCCGCACCTCCCAGCGTTAGACCAAAGAGGAGGAGGAAGAGCTA CCGCAGCTTCCTGCCTGAGAAGAGCGGTTACCCTGACATCGGCTTCCCCCTCTTCCCACTCTCCAAGGGT TTCATCAAGAGCATCCGGGGGGTCCTGACACAGTTCCGTGCTGCCCTGCTGGAGGCGGGCATGCCCGAAA GCACAGAGGACAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG220692 representing NM_173755
Red=Cloning site Green=Tags(s) MADPAAPAPAQAQAAAAPTPAAAPAAAAPPPAPATDSASGPSSDSGPEAGSQRLLFSHDLVSGRYRGSVH FGLVRLIHGEDSDSEGDDDGRGSSGCSEAGGAGHEEGRASPLRRGYVRVQWYPEGVKQHVKETKLKLEDR SVVPRDVVRHMRSTDSQCGTVIDVNIDCAVKLIGTNCIIYPVNSKDLQHIWPFMYGDYIAYDCWLGKVYD LKNQIILKLSNGARCSMNTEDGAKLYDVCPHVSDSGLFFDDSYGFYPGQVLIGPAKIFSSVQWLSGVKPV LSTKSKFRVVVEEVQVVELKVTWITKSFCPGGTDSVSPPPSIITQENLGRVKRLGCFDHAQRQLGERCLY VFPAKVEPAKIAWECPEKNCAQGEGSMAKKVKRLLKKQVVRIMSCTPDTQCPRDHSMEDPDKKGEARAGS EIGSASPEEQPDGSASPVEMQDEGSEELQETCEPLPPFLLKEGGDDGLHSAEQDADDEAADDTDDTSSVT SSASSTTSSQSGSGTGRKKSIPLSIKNLKRKHKRKKNKVTRDFKPGDRVAVEVVTTMTSADVMWQDGSVE CNIRSNDLFPVHHLDNNEFCPGDFVVDKRVQSCPDPAVYGVVQSGDHVGRTCMVKWFKLRPSGDDVELIG EEEDVSVYDIADHPDFRFRTTDIVIRIGNTEDGALPKEDEPSVGQVARVDVSSKVEVVWADNSKTIILPQ HLYNIESEIEESDYDSVEGSSSGASSDEWEDDSDSWETDNGLVDDEHPKIEELAAILPAEQPTAPEEDKG VVISEEAATAAIQGAVAMAAPVAGLMEKAGKDGPPKSFRELKEAIKILESLKNMTVEQLLTGSPTSPTVE PEKPTREKKFLDDIKKLQENLKKTLDNVAIAEEEKMEAVPDTERKEEKPEVQSPVKAEWPSETPVLCQQC GGRPGVTFTSAKGEVFSVLEFAPSNHSFKKIEFQPPEAKKFFSTVRKEMALLATSLPDGIMVKTFEDRMD LFSALIKGPTRTPYEDGLYLFDIQLPNIYPAVPPHFCYLSQCSGRLNPNLYDNGKVCVSLLGTWIGKGTE RWTSKSSLLQVLISIQGLILVNEPYYNEAGFDSDRGLQEGYENSRCYNEMALIRVVQSMTQLVRRPPEVF EQEIRQHFSVGGWRLVNRIESWLETHAMQERAQVMPNGALKDSSSLEPMAAAELSDSGREEPEDVGMAPG EASQGSDSEGGAQGPASASRDHTEQTETAPDASAPPSVRPKRRRKSYRSFLPEKSGYPDIGFPLFPLSKG FIKSIRGVLTQFRAALLEAGMPESTEDK TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_173755 |
| ORF Size | 3864 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_173755.3, NP_776116.2 |
| RefSeq Size | 5192 bp |
| RefSeq ORF | 3867 bp |
| Locus ID | 217342 |
| UniProt ID | Q6ZPJ3 |
| Gene Summary | E2/E3 hybrid ubiquitin-protein ligase that displays both E2 and E3 ligase activities and mediates monoubiquitination of target proteins. Negatively regulates TRAF6-mediated NF-kappa-B activation independently of its E2 activity. Acts as a positive regulator of BMP7 signaling by mediating monoubiquitination of SMAD6, thereby regulating adipogenesis. Mediates monoubiquitination at different sites of the nuclear localization signal (NLS) of BAP1, leading to cytoplasmic retention of BAP1. Also able to monoubiquitinate the NLS of other chromatin-associated proteins, such as INO80 and CXXC1, affecting their subcellular location. Acts as a regulator of retrograde transport by assisting the TRIM27:MAGEL2 E3 ubiquitin ligase complex to mediate 'Lys-63'-linked ubiquitination of WASHC1, leading to promote endosomal F-actin assembly.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224173 | Ube2o (untagged) - Mouse ubiquitin-conjugating enzyme E2O (Ube2o), (10ug) |
CNY 16,630.00 |
|
| MR220692 | Ube2o (Myc-DDK-tagged) - Mouse ubiquitin-conjugating enzyme E2O (Ube2o) |
CNY 8,968.00 |
|
| MR220692L3 | Lenti ORF clone of Ube2o (Myc-DDK-tagged) - Mouse ubiquitin-conjugating enzyme E2O (Ube2o) |
CNY 13,300.00 |
|
| MR220692L4 | Lenti ORF clone of Ube2o (mGFP-tagged) - Mouse ubiquitin-conjugating enzyme E2O (Ube2o) |
CNY 13,300.00 |
