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Ube2o (NM_173755) Mouse Untagged Clone
CAT＃: MC224173
Ube2o (untagged) - Mouse ubiquitin-conjugating enzyme E2O (Ube2o), (10ug)
CNY 16,630.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 9630022H21; B230113M03Rik; E2-230K; mKIAA1734 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC224173 representing NM_173755
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGATCCCGCAGCGCCCGCACCCGCCCAGGCCCAGGCCGCCGCCGCCCCGACCCCGGCCGCAGCCC CCGCCGCCGCCGCCCCGCCGCCCGCGCCCGCTACGGACTCGGCCTCAGGGCCGTCCTCCGACTCCGGCCC CGAAGCAGGCTCACAGCGCCTGCTGTTCTCCCACGACCTGGTGTCCGGCCGTTACCGCGGCTCGGTGCAC TTCGGGCTGGTACGCCTCATCCACGGCGAGGACTCGGACTCGGAGGGCGACGATGACGGCCGCGGCAGCT CGGGCTGCTCCGAGGCCGGGGGCGCGGGCCACGAGGAGGGCCGGGCCAGCCCTCTGCGGCGCGGCTACGT GCGCGTCCAGTGGTACCCGGAGGGCGTCAAGCAGCATGTGAAGGAGACCAAGCTGAAACTGGAGGACCGC TCTGTGGTGCCCCGGGATGTGGTTCGACACATGCGCTCAACTGACAGCCAGTGTGGCACGGTGATAGATG TCAACATTGACTGTGCCGTCAAGCTCATCGGCACCAACTGCATCATCTACCCTGTCAACAGCAAGGACCT CCAGCACATCTGGCCCTTCATGTATGGAGACTATATCGCCTATGACTGCTGGCTGGGGAAGGTCTATGAC TTGAAGAACCAGATCATCCTCAAGTTGTCTAATGGTGCCAGGTGCTCCATGAACACAGAAGATGGTGCCA AACTCTACGATGTCTGCCCTCACGTCAGCGACTCGGGTCTCTTCTTTGATGACTCTTATGGCTTCTACCC AGGCCAAGTCCTTATTGGCCCAGCCAAGATCTTCTCCAGTGTCCAGTGGCTCTCTGGGGTCAAGCCTGTA CTCAGCACCAAGAGCAAGTTCCGTGTGGTGGTGGAGGAGGTGCAGGTTGTAGAACTGAAAGTCACGTGGA TTACCAAGAGCTTCTGCCCCGGCGGCACGGACAGCGTTAGCCCCCCACCTTCCATCATCACTCAGGAAAA CCTAGGCAGGGTGAAGCGTCTCGGATGCTTTGACCATGCTCAGCGGCAGCTTGGGGAACGCTGTCTGTAT GTCTTCCCAGCCAAGGTAGAGCCGGCCAAGATTGCCTGGGAATGTCCAGAAAAAAACTGCGCCCAGGGGG AGGGCTCTATGGCCAAGAAGGTGAAGCGTCTGTTGAAGAAGCAAGTTGTGAGAATCATGTCCTGCACCCC AGATACCCAGTGTCCTAGGGACCATTCCATGGAAGACCCAGACAAGAAAGGGGAAGCCAGAGCCGGGAGT GAAATAGGGTCTGCTAGCCCGGAGGAGCAACCTGATGGATCAGCCAGCCCCGTGGAGATGCAGGATGAGG GGTCGGAGGAGCTTCAGGAGACATGCGAGCCACTGCCCCCCTTCCTCTTGAAGGAGGGTGGAGATGACGG GCTGCACTCGGCAGAGCAGGACGCAGATGATGAGGCGGCTGACGATACAGATGACACCAGCTCTGTGACC TCCTCCGCCAGCTCTACTACCTCCTCCCAGAGTGGCAGTGGCACTGGTCGTAAAAAGAGCATTCCCTTGT CCATCAAAAACTTGAAGCGAAAACACAAGAGGAAGAAGAATAAGGTCACCCGTGACTTCAAGCCTGGAGA CAGGGTGGCAGTGGAGGTTGTGACCACGATGACCTCAGCTGATGTGATGTGGCAAGATGGCTCTGTGGAA TGCAACATCCGCTCCAACGACCTTTTCCCTGTGCACCACCTGGACAACAATGAGTTCTGCCCTGGGGACT TCGTGGTGGACAAGCGAGTCCAGAGCTGCCCAGACCCTGCTGTCTATGGTGTGGTGCAGTCTGGGGATCA TGTTGGCCGTACCTGCATGGTGAAGTGGTTCAAGCTGCGACCAAGTGGGGATGACGTGGAGCTCATTGGA GAAGAGGAGGACGTGAGCGTCTATGACATTGCTGACCACCCTGACTTTCGCTTCCGCACAACTGACATTG TCATCCGCATTGGTAACACGGAGGATGGGGCTCTACCCAAGGAGGATGAGCCCTCAGTGGGCCAGGTGGC CCGAGTGGATGTCAGCAGCAAAGTGGAAGTGGTGTGGGCTGACAACTCGAAAACCATCATCCTGCCACAG CACTTATACAACATCGAGTCTGAGATTGAGGAGTCTGACTACGACTCCGTGGAAGGCAGCTCCAGTGGGG CCTCCTCGGATGAGTGGGAGGACGACAGCGACAGCTGGGAGACCGACAACGGGCTCGTGGACGATGAGCA CCCCAAGATAGAGGAGCTGGCCGCCATCTTGCCTGCCGAGCAGCCCACTGCCCCCGAGGAGGACAAGGGC GTGGTCATCAGTGAGGAGGCGGCCACGGCTGCCATCCAGGGCGCCGTGGCCATGGCTGCCCCCGTGGCAG GGCTGATGGAGAAAGCTGGGAAGGACGGGCCTCCCAAGAGTTTCCGGGAGCTGAAGGAGGCCATCAAGAT CCTGGAGAGCCTCAAGAACATGACTGTGGAGCAGCTGCTCACAGGCTCGCCCACCTCGCCCACCGTGGAG CCTGAGAAGCCAACCCGAGAGAAGAAGTTTTTGGACGATATCAAGAAGCTACAGGAGAACCTCAAGAAGA CCCTGGACAATGTGGCCATTGCTGAGGAGGAGAAGATGGAGGCAGTACCCGACACAGAGCGCAAGGAGGA GAAGCCTGAGGTGCAGTCCCCAGTGAAAGCCGAGTGGCCCAGTGAGACACCTGTGCTCTGTCAGCAGTGC GGTGGCAGGCCTGGCGTCACCTTCACCAGCGCCAAGGGCGAGGTCTTCTCTGTGCTGGAGTTTGCACCCT CAAACCACTCTTTCAAGAAGATTGAGTTCCAGCCCCCGGAAGCCAAGAAGTTCTTCAGCACAGTGCGGAA GGAGATGGCTCTGCTGGCCACCTCGCTGCCCGATGGCATCATGGTCAAGACTTTTGAGGACAGAATGGAC CTCTTCTCTGCACTGATCAAGGGCCCCACTCGGACTCCCTATGAAGACGGCCTCTACCTATTTGACATCC AGCTTCCCAACATCTACCCTGCGGTGCCCCCCCACTTTTGCTACCTCTCCCAGTGCAGTGGCCGCCTGAA CCCCAACCTGTATGACAATGGGAAAGTGTGTGTCAGCCTCCTAGGTACCTGGATTGGAAAGGGGACAGAG AGGTGGACAAGCAAGTCTAGCCTTCTCCAGGTGCTCATCTCCATCCAAGGTCTGATCCTGGTGAATGAAC CATACTACAATGAAGCTGGCTTTGACAGTGACCGGGGCCTGCAGGAAGGCTATGAGAACAGTCGCTGCTA CAACGAGATGGCATTAATCCGTGTGGTGCAGTCCATGACCCAGTTGGTGCGACGACCTCCTGAGGTCTTC GAACAGGAGATTCGGCAGCACTTCAGTGTGGGTGGCTGGCGGCTGGTGAACCGAATTGAGTCCTGGCTGG AAACCCATGCCATGCAGGAGAGGGCCCAGGTGATGCCCAATGGGGCCCTTAAAGACAGCAGCTCTCTGGA GCCCATGGCTGCAGCTGAACTCTCAGACTCCGGCCGTGAAGAGCCTGAAGATGTTGGGATGGCCCCTGGG GAGGCGTCCCAGGGCTCAGACTCAGAGGGTGGAGCCCAGGGCCCTGCCTCAGCCAGCAGAGACCACACAG AGCAGACTGAGACAGCCCCTGATGCATCCGCACCTCCCAGCGTTAGACCAAAGAGGAGGAGGAAGAGCTA CCGCAGCTTCCTGCCTGAGAAGAGCGGTTACCCTGACATCGGCTTCCCCCTCTTCCCACTCTCCAAGGGT TTCATCAAGAGCATCCGGGGGGTCCTGACACAGTTCCGTGCTGCCCTGCTGGAGGCGGGCATGCCCGAAA GCACAGAGGACAAGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_173755 |
| Insert Size | 3867 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_173755.3, NP_776116.2 |
| RefSeq Size | 5192 bp |
| RefSeq ORF | 3867 bp |
| Locus ID | 217342 |
| UniProt ID | Q6ZPJ3 |
| Gene Summary | E2/E3 hybrid ubiquitin-protein ligase that displays both E2 and E3 ligase activities and mediates monoubiquitination of target proteins. Negatively regulates TRAF6-mediated NF-kappa-B activation independently of its E2 activity. Acts as a positive regulator of BMP7 signaling by mediating monoubiquitination of SMAD6, thereby regulating adipogenesis. Mediates monoubiquitination at different sites of the nuclear localization signal (NLS) of BAP1, leading to cytoplasmic retention of BAP1. Also able to monoubiquitinate the NLS of other chromatin-associated proteins, such as INO80 and CXXC1, affecting their subcellular location. Acts as a regulator of retrograde transport by assisting the TRIM27:MAGEL2 E3 ubiquitin ligase complex to mediate 'Lys-63'-linked ubiquitination of WASHC1, leading to promote endosomal F-actin assembly.[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG220692 | Ube2o (tGFP-tagged) - Mouse ubiquitin-conjugating enzyme E2O (Ube2o), (10ug) |
CNY 12,540.00 |
|
| MR220692 | Ube2o (Myc-DDK-tagged) - Mouse ubiquitin-conjugating enzyme E2O (Ube2o) |
CNY 8,968.00 |
|
| MR220692L3 | Lenti ORF clone of Ube2o (Myc-DDK-tagged) - Mouse ubiquitin-conjugating enzyme E2O (Ube2o) |
CNY 13,300.00 |
|
| MR220692L4 | Lenti ORF clone of Ube2o (mGFP-tagged) - Mouse ubiquitin-conjugating enzyme E2O (Ube2o) |
CNY 13,300.00 |
