Snx27 (NM_029721) Mouse Tagged ORF Clone
CAT#: MG218832
- TrueORF®
Snx27 (tGFP-tagged) - Mouse sorting nexin family member 27 (Snx27) transcript variant 2, (10ug)
ORF Plasmid: DDK
"NM_029721" in other vectors (4)
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CNY 5,040.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 5730552M22Rik; ESTM45; ESTM47; R75405 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG218832 representing NM_029721
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGACGAGGACGGGGAAGGTATTCACCCCTCAGCCCCGCACCGGAATGGAGGCGGCGGCGGGGGGT CTGGGCTCCACTGCGCCGGGAACGGTGGCGGGGGAGGCGGCGGCCCGCGGGTCGTGCGCATTGTCAAGTC CGAATCCGGCTACGGCTTCAACGTGCGGGGCCAAGTGAGCGAGGGCGGGCAACTGCGGAGCATCAACGGG GAGCTGTACGCGCCGCTGCAGCATGTGAGCGCCGTGCTGCCCGGGGGGGCGGCCGACCGGGCCGGGGTGC GCAAAGGGGACCGCATCCTGGAGGTGAACGGCGTGAATGTTGAGGGGGCGACACACAAGCAGGTGGTGGA CCTGATCCGAGCAGGCGAGAAGGAATTGATCTTGACAGTGTTGTCTGTACCTCCTCATGAGGCTGATAAC CTAGATCCCAGTGACGACTCCTTGGGACAATCATTTTATGATTACACAGAAAAGCAAGCAGTGCCCATAT CGGTCCCCACATACAAACATGTGGAGCAGAATGGTGAGAAGTTTGTGGTATACAATGTTTACATGGCTGG GAGGCAGCTGTGTTCTAAGCGGTACCGGGAGTTTGCCATACTGCACCAAAACCTGAAGAGAGAATTTGCC AACTTTACATTTCCTCGACTTCCAGGAAAGTGGCCATTCTCATTATCAGAACAACAATTGGATGCCCGGC GTCGTGGGTTGGAAGAATATCTAGAAAAGGTGTGTTCAATCCGAGTCATTGGTGAGAGTGACATCATGCA GGAATTCCTGTCTGAGTCTGATGAGAATTACAATGGTGTGTCTGATGTAGAACTGAGAGTAGCACTACCG GATGGAACAACGGTTACTGTCAGAGTTAAGAAGAACAGTACTACAGACCAAGTGTACCAGGCTATTGCAG CAAAGGTTGGCATGGACAGTACAACAGTGAATTACTTTGCCTTATTTGAAGTGATCAATCATTCCTTTGT ACGTAAGCTGGCACCTAATGAATTTCCTCATAAACTCTACGTCCAGAACTATACATCAGCTGTGCCAGGC ACTTGCCTGACCATTCGCAAGTGGCTCTTTACAACAGAAGAAGAAGTCCTCTTAAATGACAATGACCTTG CTGTTACGTACTTCTTTCATCAGGCTGTTGATGATGTAAAGAAAGGTTACATTAAAGCAGAAGAGAAATC TTACCAGTTACAGAAGCTCCATGAACAAAGGAAGATGGTCATGTACCTCAACATGCTAAGGACCTGCGAG GGCTACAATGAAATCATCTTCCCTCACTGTGCCTGTGACTCGAGGAGGAAGGGACATGTTATCACAGCCA TCAGCATCACGCACTTTAAACTTCATGCATGCACTGAAGAAGGGCAGCTGGAGAACCAGGTAATAGCGTT TGAATGGGATGAGATGCAGCGATGGGACACAGATGAAGAAGGAATGGCCTTCTGTTTTGAATATGCGCGA GGAGAGAAGAAGCCTCGATGGGTTAAAATCTTCACACCATATTTCAATTACATGCATGAATGCTTTGAGA GGGTGTTCTGCGAGCTCAAGTGGAGAAAAGAGGAATAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG218832 representing NM_029721
Red=Cloning site Green=Tags(s) MADEDGEGIHPSAPHRNGGGGGGSGLHCAGNGGGGGGGPRVVRIVKSESGYGFNVRGQVSEGGQLRSING ELYAPLQHVSAVLPGGAADRAGVRKGDRILEVNGVNVEGATHKQVVDLIRAGEKELILTVLSVPPHEADN LDPSDDSLGQSFYDYTEKQAVPISVPTYKHVEQNGEKFVVYNVYMAGRQLCSKRYREFAILHQNLKREFA NFTFPRLPGKWPFSLSEQQLDARRRGLEEYLEKVCSIRVIGESDIMQEFLSESDENYNGVSDVELRVALP DGTTVTVRVKKNSTTDQVYQAIAAKVGMDSTTVNYFALFEVINHSFVRKLAPNEFPHKLYVQNYTSAVPG TCLTIRKWLFTTEEEVLLNDNDLAVTYFFHQAVDDVKKGYIKAEEKSYQLQKLHEQRKMVMYLNMLRTCE GYNEIIFPHCACDSRRKGHVITAISITHFKLHACTEEGQLENQVIAFEWDEMQRWDTDEEGMAFCFEYAR GEKKPRWVKIFTPYFNYMHECFERVFCELKWRKEEY TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_029721 |
ORF Size | 1578 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_029721.2, NP_083997.1 |
RefSeq Size | 6259 bp |
RefSeq ORF | 1581 bp |
Locus ID | 76742 |
UniProt ID | Q3UHD6 |
Gene Summary | Involved in the retrograde transport from endosome to plasma membrane, a trafficking pathway that promotes the recycling of internalized transmembrane proteins. Following internalization, endocytosed transmembrane proteins are delivered to early endosomes and recycled to the plasma membrane instead of being degraded in lysosomes. SNX27 specifically binds and directs sorting of a subset of transmembrane proteins containing a PDZ-binding motif at the C-terminus: following interaction with target transmembrane proteins, associates with the retromer complex, preventing entry into the lysosomal pathway, and promotes retromer-tubule based plasma membrane recycling. SNX27 also binds with the WASH complex. Interacts with membranes containing phosphatidylinositol-3-phosphate (PtdIns(3P)). May participate in establishment of natural killer cell polarity. Recruits CYTIP to early endosomes.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC217483 | Snx27 (untagged) - Mouse sorting nexin family member 27 (Snx27), transcript variant 2, (10ug) |
CNY 6,750.00 |
|
MR218832 | Snx27 (Myc-DDK-tagged) - Mouse sorting nexin family member 27 (Snx27), transcript variant 2 |
CNY 3,920.00 |
|
MR218832L3 | Lenti ORF clone of Snx27 (Myc-DDK-tagged) - Mouse sorting nexin family member 27 (Snx27), transcript variant 2 |
CNY 6,460.00 |
|
MR218832L4 | Lenti ORF clone of Snx27 (mGFP-tagged) - Mouse sorting nexin family member 27 (Snx27), transcript variant 2 |
CNY 6,460.00 |