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Snx27 (NM_029721) Mouse Untagged Clone
CAT＃: MC217483
Snx27 (untagged) - Mouse sorting nexin family member 27 (Snx27), transcript variant 2, (10ug)
CNY 6,750.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 5730552M22Rik; ESTM45; ESTM47; R75405 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC217483 representing NM_029721
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGACGAGGACGGGGAAGGTATTCACCCCTCAGCCCCGCACCGGAATGGAGGCGGCGGCGGGGGGT CTGGGCTCCACTGCGCCGGGAACGGTGGCGGGGGAGGCGGCGGCCCGCGGGTCGTGCGCATTGTCAAGTC CGAATCCGGCTACGGCTTCAACGTGCGGGGCCAAGTGAGCGAGGGCGGGCAACTGCGGAGCATCAACGGG GAGCTGTACGCGCCGCTGCAGCATGTGAGCGCCGTGCTGCCCGGGGGGGCGGCCGACCGGGCCGGGGTGC GCAAAGGGGACCGCATCCTGGAGGTGAACGGCGTGAATGTTGAGGGGGCGACACACAAGCAGGTGGTGGA CCTGATCCGAGCAGGCGAGAAGGAATTGATCTTGACAGTGTTGTCTGTACCTCCTCATGAGGCTGATAAC CTAGATCCCAGTGACGACTCCTTGGGACAATCATTTTATGATTACACAGAAAAGCAAGCAGTGCCCATAT CGGTCCCCACATACAAACATGTGGAGCAGAATGGTGAGAAGTTTGTGGTATACAATGTTTACATGGCTGG GAGGCAGCTGTGTTCTAAGCGGTACCGGGAGTTTGCCATACTGCACCAAAACCTGAAGAGAGAATTTGCC AACTTTACATTTCCTCGACTTCCAGGAAAGTGGCCATTCTCATTATCAGAACAACAATTGGATGCCCGGC GTCGTGGGTTGGAAGAATATCTAGAAAAGGTGTGTTCAATCCGAGTCATTGGTGAGAGTGACATCATGCA GGAATTCCTGTCTGAGTCTGATGAGAATTACAATGGTGTGTCTGATGTAGAACTGAGAGTAGCACTACCG GATGGAACAACGGTTACTGTCAGAGTTAAGAAGAACAGTACTACAGACCAAGTGTACCAGGCTATTGCAG CAAAGGTTGGCATGGACAGTACAACAGTGAATTACTTTGCCTTATTTGAAGTGATCAATCATTCCTTTGT ACGTAAGCTGGCACCTAATGAATTTCCTCATAAACTCTACGTCCAGAACTATACATCAGCTGTGCCAGGC ACTTGCCTGACCATTCGCAAGTGGCTCTTTACAACAGAAGAAGAAGTCCTCTTAAATGACAATGACCTTG CTGTTACGTACTTCTTTCATCAGGCTGTTGATGATGTAAAGAAAGGTTACATTAAAGCAGAAGAGAAATC TTACCAGTTACAGAAGCTCCATGAACAAAGGAAGATGGTCATGTACCTCAACATGCTAAGGACCTGCGAG GGCTACAATGAAATCATCTTCCCTCACTGTGCCTGTGACTCGAGGAGGAAGGGACATGTTATCACAGCCA TCAGCATCACGCACTTTAAACTTCATGCATGCACTGAAGAAGGGCAGCTGGAGAACCAGGTAATAGCGTT TGAATGGGATGAGATGCAGCGATGGGACACAGATGAAGAAGGAATGGCCTTCTGTTTTGAATATGCGCGA GGAGAGAAGAAGCCTCGATGGGTTAAAATCTTCACACCATATTTCAATTACATGCATGAATGCTTTGAGA GGGTGTTCTGCGAGCTCAAGTGGAGAAAAGAGGAATATTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_029721 |
| Insert Size | 1581 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_029721.2, NP_083997.1 |
| RefSeq Size | 6273 bp |
| RefSeq ORF | 1581 bp |
| Locus ID | 76742 |
| UniProt ID | Q3UHD6 |
| Gene Summary | Involved in the retrograde transport from endosome to plasma membrane, a trafficking pathway that promotes the recycling of internalized transmembrane proteins. Following internalization, endocytosed transmembrane proteins are delivered to early endosomes and recycled to the plasma membrane instead of being degraded in lysosomes. SNX27 specifically binds and directs sorting of a subset of transmembrane proteins containing a PDZ-binding motif at the C-terminus: following interaction with target transmembrane proteins, associates with the retromer complex, preventing entry into the lysosomal pathway, and promotes retromer-tubule based plasma membrane recycling. SNX27 also binds with the WASH complex. Interacts with membranes containing phosphatidylinositol-3-phosphate (PtdIns(3P)). May participate in establishment of natural killer cell polarity. Recruits CYTIP to early endosomes.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses an alternate 3' terminal exon which results in a frameshift compared to variant 1. It encodes isoform 2 which has a shorter and distinct C-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG218832 | Snx27 (tGFP-tagged) - Mouse sorting nexin family member 27 (Snx27) transcript variant 2, (10ug) |
CNY 5,040.00 |
|
| MR218832 | Snx27 (Myc-DDK-tagged) - Mouse sorting nexin family member 27 (Snx27), transcript variant 2 |
CNY 3,920.00 |
|
| MR218832L3 | Lenti ORF clone of Snx27 (Myc-DDK-tagged) - Mouse sorting nexin family member 27 (Snx27), transcript variant 2 |
CNY 6,460.00 |
|
| MR218832L4 | Lenti ORF clone of Snx27 (mGFP-tagged) - Mouse sorting nexin family member 27 (Snx27), transcript variant 2 |
CNY 6,460.00 |
