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Psme4 (NM_134013) Mouse Tagged ORF Clone
CAT＃: MG218061
- TrueORF®
Psme4 (tGFP-tagged) - Mouse proteasome (prosome macropain) activator subunit 4 (Psme4), (10ug)
ORF Plasmid: DDK
"NM_134013" in other vectors (2)
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CNY 15,580.00
货期*
9周
规格
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经常一起买 (4)
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AA409398; AU041366; mKIAA0077; TEMO |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG218061 representing NM_134013
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGCTCCGGAGCGGGCGGGCGGCGGGGAGCCCCCCGAGCCTGGCGGGCGGCCGGTACTGGGCCCGC GGGCCTTCGTCCCGCAGAAGGAGATCGTGTACAACAAGCTTCTGCCCTATGCCGAGCGGCTGGACGCCGA GTCCGACTTGCAGCTGGCACAGATCAAGAGCAACCTGGGCCGGGCCGTGCAGCTCCAAGAGCTGTGGCCC GGGGGCCTCTTCTGGACGCGGAAACTCTCCACTTATATCCGACTTTATGGGAGAAAATTTAGCAAAGAAG ATCATGTTCTTTTTATTAAGTTACTGTATGAGCTGGTATCAATTCCAAAACTAGAAATCAGCATGATGCA GGGATTTGCCCGTCTCTTGATCAACTTGTTAAAAAAAAAGGAACTTCTTTCAAGAGATGATTTAGAGTTA CCTTGGAGACCACTTTATGACTTGGTAGAAAGAATATTATATTCCAAGACAGAGCACCTGAGATTAAATT CATTTCCTAATTCTATAGAAAATGTTCTCAAAACACTGGTGAAAAGCTGCCGACCATACTTTCCAGCAGA TTCAACAGCAGAAATGTTAGAAGAATGGCGGCCTTTAATGTGTCCTTTTGATGTAACAATGCAAAAGGCC ATCAGTTATTTTGAAATATTTCTTCCTACCTCCCTTCCTCCAGAACTGCATCATAAAGGTTTTAAACTTT GGTTTGATGAATTAATTGGCCTTTGGGTTTCAGTGCAAAATCTCCCACAGTGGGAGGGGCAATTAGTAAA TCTCTTTGCTCGATTGGCTACAGATAATATAGGATACATAGATTGGGATCCATATGTACCGAAGATATTT ACAAGAATTCTGAGAAGCCTGAACCTCCCAGTGGGAAGCAGTCAAGTTTTAGTCCCAAGATTTTTAACAA ATGCTTATGATATAGGACATGCTGTAATATGGATCACCGCAATGATGGGTGGACCAAGTAAATTAGTACA AAAACATTTAGCTGGTTTGTTTAACAGCATCACATCATTTTATCATCCTTCAAATAATGGGCGTTGGCTG AACAAGTTAATGAAACTCCTTCAGCGGTTGCCAAACAGTGTTGTTAGGAGATTACATCGTGAAAGATTCA AGAAGCCCTCCTGGTTAACTCCTGTGCCTGAAAGCCATAAGCTTACTGATGAAGATGTTACAGACTTTGT ACAATGCATTATTCAGCCTGTCCTCTTGGCTATGTTTAGCAAAACTGGTAGTCTAGAAGCAGCCCAGGCT CTGCAGAACCTTGCACTTATGAGACCTGAGTTAGTAATACCCCCTGTGCTTGAAAGAACATACCCTGCTT TAGAGACATTAACAGAACCTCACCAGCTCACAGCTACTTTAAATTGTGTAATTGGAGTAGCCCGAAGTTT GGTATCAGGCAGCAAATGGTTTCCCGAAGGGCCAACACACATGCTACCTCTGTTGATGAGAGCATTGCCT GGGGTAGATCCAAACGACTTTAGTAAGTGCATGATCACATTCCAATTTATAGGAACATTTTCTACTCTGG TGCCTTTAGTAGACTGTTCATCTGTACTGCAAGAAAGAAATGACCTCACAGAAATTGAAAAAGAACTTTG TTCGGCAACAGCTGAATTTGAGGATTTTGTGTTACAATTTATGGACAGATGCTTTGGACTAATAGAAAGT AGTACATTGGAGCAAACAAGGGAAGAGACAGAAACAGAGAAAATGACTCATTTGGAGAGTTTGGTTGAAT TAGGCTTATCTTCTACGTTTAGTACAATCCTCACACAATGTTCCAAAGACATATTTATGGTGGCCCTTCA GAAGGTCTTTAACTTTTCTGTTTCACATATATTTGAAACAAGAGTTGCAGGTCGCATGGTGGCAGACATG TGCCGAGCTGCTGTGAAGTGTTGCCCAGAAGAATCCCTGAAGCTGTTTGTTCCCCACTGCTATGGTGTTA TAACTCAGCTTACAATGAATGATGATGTATTGAATGAAGAAGAGCTAGACAAGGAGTTGCTGTGGAACCT TCAGCTTCTGTCTGAGATTACTCGTGTAGACGGGAAGAAGTTACTTCTGTATAGGGAGCAGCTTGTAAAG ATTCTCCAGCGAACCTTACACCTGACCTGTAAGCAGGGCTACACGCTGTCTTGCAACCTTCTGCATCATC TTCTTCGTTCTACCACACTAATCTACCCTACAGAGTACTGCAGTGTTCCGGGGGGCTTTAACAAGCCGCC TTCTGAATACTTCCCTGTCAAGGACTGGGGTAAGCCTGGAGATTTGTGGAATCTGGGGATCCAGTGGCAT GTTCCTTCTTCAGAAGAAGTCTCTTTTGCCTTCTATTTGTTAGACTCCTTTCTTCAGCCTGAGCTTATCA AACTCCAGTGTTGTGGAGATGGAGAACTTGAAATGTCTAGAGATGATATTCTACAGAGTCTGACTATAGT ACACAGCTGCTTGATTGGTTCAGGGAACCTTCTACCTCCATTGAAAGGAGAGGCAGTTACTAACTTGGTA CCAAGTATGGTCTCCTTAGAGGAAACAAAATTGTATACCGGACTGGAACATGACCTATCCAGAGAAAACT ACCGAGAAGTAATTGCTAGTGTCATAAGGAAACTTCTTAGCCACATACTTGATAATTCAGAAGATGATAC CAAATCACTGTTTCTCATCATAAAGATCATTGGAGACCTTCTGCACTTCCAAGGATCTCATAAACATGAA TTTGACTCTCGATGGAAAAGCTTTAACTTAGTAAAGAAATCAATGGAAAATCGGCTCCATGGAAAAAAGC AACATATCAGAGCATTACTAATTGACAGAGTGATGCTGCAGCATGAGTTGCGAACACTAACTGTTGAGGG TTGTGAGTACAAAAAAATACATCAAGATATGATCAGAGATCTTCTTCGATTATCAACAAGCTCATACAGT CAGGTCAGAAACAAAGCTCAGCAAACATTTTTTGCTGCTTTGGGGGCATATAACTTCTGTTGCAGAGATA TTATCCCCTTGGTTTTGGAATTCCTACGGCCTGATAGAAAGGATGTCACACAGCAGCAGTTCAAGGGCGC CTTATATTGTCTACTTGGAAATCACAGTGGTGTGTGCCTGGCAAACCTTCATGATTGGGACTGTATTGTG CAGACCTGGCCAGCACTTGTTTCTTCAGGGCTTAGCCAAGCAATGTCACTGGAAAAGCCATCAATAGTGA GACTATTTGATGACCTTGCTGAAAAGATTCATAGACAATATGAAACAATTGGCTTGGATTTTACAATTCC AAAGTCATGTGCTGCAATAGCAGAATTACTTCAACAATCAAAGAATCCTTCCATTAGCCAGACACTACTT AGCCCAGAAAAGATTAAAGAAGGACAGAAGCGTCAACAAGATAAGAATGCTGATGCCCTAAGGAACTATG AATGTTTGGTAAATACTTTACTAGATGGTGTGGAGCAAAGAAATCTGCCTTGGAAATTTGAGCATATAGG AATCGGGCTTCTGTCTCTACTCCTAAGGGATGACAGAGTGTTGCCTCTTCGTGCCATACGTTTTTTTGTT GAAAATCTCAACCATGATGCAATTGTTGTTCGAAAGATGGCTATCTCAGCTGTTGCTGGTATTCTCAAGC AGCTAAAGAGAACTCACAAAAAGCTGACTATCAACCCTTATGAAATCAGTGGATGTCCTAAACCTACCAA AATTCTTGCTGGTGATAGACCTGATAACCATTGGTTGCATTATGATAGTAAAAATATACCAAGAACAAAA AAAGAATGGGAGTCAAGTTGCTTTGTTGAAAAAACTCACTGGGGATACTACAACTGGCCAAAGAATATGG TTGTTTATGCTGGTGTGGAAGAACAGCCTAAACTTGGCAGAAGCAGGGAGGATATGATAGAGGCAGAACA GATTATATATGATCGTTTTTCTGATCCTAAGTTTGTTGAGCAGTTAATCACATTTCTATCTTTAGAAGAC AGAAAAGGAAGAGATAAGTTTAGTCCCCGGCGTTTCTGCCTCTTTAAGGGTATATTTAGAAATTTTGATG ATGCTTTTCTGCCTGTTCTGAAGCCCCATTTAGAACGTTTGGTTGCAGATTCTCATGAAAGTACCCAGAG ATGTGTTGCAGAAATTATAGCTGGTTTAATCAGAGGTTCTAAGCATTGGACGTTTGAGAAGGTGGAGAAG CTTTGGGAGCTCCTGTGCCCGCTGCTGAGAACGGCACTGTCCAACATGACAGTGGAGACATACAATGACT GGGGGACCTGTATAGCCACTTCCTGTGAAAGCAGAGATCCCCGGAAGCTTCACTGGCTTTTTGAGCTGCT CTTGGAGTCACCATTGAGTGGTGAAGGGGGGTCCTTTGTAGATGCATGCCGACTCTATGTGCTACAAGGT GGCCTTGCCCAGCAAGAGTGGAGAGTCCCTGAACTATTGCACAGACTTCTGAAGTACTTGGAACCAAAAC TCACCCAGGTTTACAAAAATGTCAGAGAAAGAATAGGAAGTGTGCTGACCTACATATTTATGATAGATGT TTCTTTGCCAAATACTGCTCCAACAACATCTCCTTGTATCCCTGAGTTTACTGCTCGCGTCCTAGAAAAA CTGAAACCACTCACAGATGTGGATGAAGAAATTCAGAACCATGTTATGGAAGAAAATGGCATTGGTGAAG AAGATGAACGAACTCAGGGCATTAAACTCTTAAAAACTATATTGAAATGGCTTATGGCAAGTGCAGGAAG ATCTTTTTCTACAGCAGTCAAAGAGCAACTTCAGCTTCTGCCTTTGTTCTTTAAGATCGCTCCAGTGGAA AATGACAATAGTTATGATGAACTGAAAAGAGATGCAAAGCTCTGTTTGTCACTAATGTCTCAGGGGTTGC TGTACCCTCAACAAGTGCCTTTGATACTTCAGGTGCTAAGCCAGACAGCAAGAAGCAGTTCTTGGCATGC AAGATATACAGTGCTGACCTACCTCCAGACCATGGTATTTTATAACCTCTTTATTTTCCTAAACAATGAA GATGCAGTTAAAGACATCAGATGGCTGATTATATGCCTTTTGGAGGATGAACAGTTAGAGGTTCGAGAAA TGGCTGCTACCACCTTAAGTGGTCTGTTACAGTGTAACTTCCTTACCATGGACAGTGCTATGCAGATTCA TTTTGAGCAACTTTGCAAAACAAAACTACCCAAGAAAAGAAAACGAGACCCTGGTTCTGTGGGAGATACC ATTCCTTCTGCAGAGTTGGTCAAACGTCATGCTGGTGTGCTTGGACTTGGTGCATGTGTTCTTTCTAGCC CTTATGATGTTCCTACCTGGATGCCCCAGCTCCTTATGAACCTCAGTGCACATTTGAATGATCCTCAGCC TATTGAGATGACTGTAAAGAAGACCTTATCCAATTTCCGAAGGACACATCATGACAACTGGCAAGAACAC AAGCAGCAGTTCACTGATGACCAGCTGCTTGTCCTCACTGACCTCCTTGTGTCACCATGCTATTATGCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG218061 representing NM_134013
Red=Cloning site Green=Tags(s) MEAPERAGGGEPPEPGGRPVLGPRAFVPQKEIVYNKLLPYAERLDAESDLQLAQIKSNLGRAVQLQELWP GGLFWTRKLSTYIRLYGRKFSKEDHVLFIKLLYELVSIPKLEISMMQGFARLLINLLKKKELLSRDDLEL PWRPLYDLVERILYSKTEHLRLNSFPNSIENVLKTLVKSCRPYFPADSTAEMLEEWRPLMCPFDVTMQKA ISYFEIFLPTSLPPELHHKGFKLWFDELIGLWVSVQNLPQWEGQLVNLFARLATDNIGYIDWDPYVPKIF TRILRSLNLPVGSSQVLVPRFLTNAYDIGHAVIWITAMMGGPSKLVQKHLAGLFNSITSFYHPSNNGRWL NKLMKLLQRLPNSVVRRLHRERFKKPSWLTPVPESHKLTDEDVTDFVQCIIQPVLLAMFSKTGSLEAAQA LQNLALMRPELVIPPVLERTYPALETLTEPHQLTATLNCVIGVARSLVSGSKWFPEGPTHMLPLLMRALP GVDPNDFSKCMITFQFIGTFSTLVPLVDCSSVLQERNDLTEIEKELCSATAEFEDFVLQFMDRCFGLIES STLEQTREETETEKMTHLESLVELGLSSTFSTILTQCSKDIFMVALQKVFNFSVSHIFETRVAGRMVADM CRAAVKCCPEESLKLFVPHCYGVITQLTMNDDVLNEEELDKELLWNLQLLSEITRVDGKKLLLYREQLVK ILQRTLHLTCKQGYTLSCNLLHHLLRSTTLIYPTEYCSVPGGFNKPPSEYFPVKDWGKPGDLWNLGIQWH VPSSEEVSFAFYLLDSFLQPELIKLQCCGDGELEMSRDDILQSLTIVHSCLIGSGNLLPPLKGEAVTNLV PSMVSLEETKLYTGLEHDLSRENYREVIASVIRKLLSHILDNSEDDTKSLFLIIKIIGDLLHFQGSHKHE FDSRWKSFNLVKKSMENRLHGKKQHIRALLIDRVMLQHELRTLTVEGCEYKKIHQDMIRDLLRLSTSSYS QVRNKAQQTFFAALGAYNFCCRDIIPLVLEFLRPDRKDVTQQQFKGALYCLLGNHSGVCLANLHDWDCIV QTWPALVSSGLSQAMSLEKPSIVRLFDDLAEKIHRQYETIGLDFTIPKSCAAIAELLQQSKNPSISQTLL SPEKIKEGQKRQQDKNADALRNYECLVNTLLDGVEQRNLPWKFEHIGIGLLSLLLRDDRVLPLRAIRFFV ENLNHDAIVVRKMAISAVAGILKQLKRTHKKLTINPYEISGCPKPTKILAGDRPDNHWLHYDSKNIPRTK KEWESSCFVEKTHWGYYNWPKNMVVYAGVEEQPKLGRSREDMIEAEQIIYDRFSDPKFVEQLITFLSLED RKGRDKFSPRRFCLFKGIFRNFDDAFLPVLKPHLERLVADSHESTQRCVAEIIAGLIRGSKHWTFEKVEK LWELLCPLLRTALSNMTVETYNDWGTCIATSCESRDPRKLHWLFELLLESPLSGEGGSFVDACRLYVLQG GLAQQEWRVPELLHRLLKYLEPKLTQVYKNVRERIGSVLTYIFMIDVSLPNTAPTTSPCIPEFTARVLEK LKPLTDVDEEIQNHVMEENGIGEEDERTQGIKLLKTILKWLMASAGRSFSTAVKEQLQLLPLFFKIAPVE NDNSYDELKRDAKLCLSLMSQGLLYPQQVPLILQVLSQTARSSSWHARYTVLTYLQTMVFYNLFIFLNNE DAVKDIRWLIICLLEDEQLEVREMAATTLSGLLQCNFLTMDSAMQIHFEQLCKTKLPKKRKRDPGSVGDT IPSAELVKRHAGVLGLGACVLSSPYDVPTWMPQLLMNLSAHLNDPQPIEMTVKKTLSNFRRTHHDNWQEH KQQFTDDQLLVLTDLLVSPCYYA TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_134013 |
| ORF Size | 5529 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_134013.3, NP_598774.2 |
| RefSeq Size | 6454 bp |
| RefSeq ORF | 5532 bp |
| Locus ID | 103554 |
| UniProt ID | Q5SSW2 |
| Gene Summary | Associated component of the proteasome that specifically recognizes acetylated histones and promotes ATP- and ubiquitin-independent degradation of core histones during spermatogenesis and DNA damage response. Recognizes and binds acetylated histones via its bromodomain-like (BRDL) region and activates the proteasome by opening the gated channel for substrate entry. Binds to the core proteasome via its C-terminus, which occupies the same binding sites as the proteasomal ATPases, opening the closed structure of the proteasome via an active gating mechanism. Component of the spermatoproteasome, a form of the proteasome specifically found in testis: binds to acetylated histones and promotes degradation of histones, thereby participating actively to the exchange of histones during spermatogenesis. Also involved in DNA damage response in somatic cells, by promoting degradation of histones following DNA double-strand breaks.[UniProtKB/Swiss-Prot Function] |
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