Psme4 (NM_134013) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AA409398; AU041366; mKIAA0077; TEMO
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224972 representing NM_134013 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGCTCCGGAGCGGGCGGGCGGCGGGGAGCCCCCCGAGCCTGGCGGGCGGCCGGTACTGGGCCCGC GGGCCTTCGTCCCGCAGAAGGAGATCGTGTACAACAAGCTTCTGCCCTATGCCGAGCGGCTGGACGCCGA GTCCGACTTGCAGCTGGCACAGATCAAGAGCAACCTGGGCCGGGCCGTGCAGCTCCAAGAGCTGTGGCCC GGGGGCCTCTTCTGGACGCGGAAACTCTCCACTTATATCCGACTTTATGGGAGAAAATTTAGCAAAGAAG ATCATGTTCTTTTTATTAAGTTACTGTATGAGCTGGTATCAATTCCAAAACTAGAAATCAGCATGATGCA GGGATTTGCCCGTCTCTTGATCAACTTGTTAAAAAAAAAGGAACTTCTTTCAAGAGATGATTTAGAGTTA CCTTGGAGACCACTTTATGACTTGGTAGAAAGAATATTATATTCCAAGACAGAGCACCTGAGATTAAATT CATTTCCTAATTCTATAGAAAATGTTCTCAAAACACTGGTGAAAAGCTGCCGACCATACTTTCCAGCAGA TTCAACAGCAGAAATGTTAGAAGAATGGCGGCCTTTAATGTGTCCTTTTGATGTAACAATGCAAAAGGCC ATCAGTTATTTTGAAATATTTCTTCCTACCTCCCTTCCTCCAGAACTGCATCATAAAGGTTTTAAACTTT GGTTTGATGAATTAATTGGCCTTTGGGTTTCAGTGCAAAATCTCCCACAGTGGGAGGGGCAATTAGTAAA TCTCTTTGCTCGATTGGCTACAGATAATATAGGATACATAGATTGGGATCCATATGTACCGAAGATATTT ACAAGAATTCTGAGAAGCCTGAACCTCCCAGTGGGAAGCAGTCAAGTTTTAGTCCCAAGATTTTTAACAA ATGCTTATGATATAGGACATGCTGTAATATGGATCACCGCAATGATGGGTGGACCAAGTAAATTAGTACA AAAACATTTAGCTGGTTTGTTTAACAGCATCACATCATTTTATCATCCTTCAAATAATGGGCGTTGGCTG AACAAGTTAATGAAACTCCTTCAGCGGTTGCCAAACAGTGTTGTTAGGAGATTACATCGTGAAAGATTCA AGAAGCCCTCCTGGTTAACTCCTGTGCCTGAAAGCCATAAGCTTACTGATGAAGATGTTACAGACTTTGT ACAATGCATTATTCAGCCTGTCCTCTTGGCTATGTTTAGCAAAACTGGTAGTCTAGAAGCAGCCCAGGCT CTGCAGAACCTTGCACTTATGAGACCTGAGTTAGTAATACCCCCTGTGCTTGAAAGAACATACCCTGCTT TAGAGACATTAACAGAACCTCACCAGCTCACAGCTACTTTAAATTGTGTAATTGGAGTAGCCCGAAGTTT GGTATCAGGCAGCAAATGGTTTCCCGAAGGGCCAACACACATGCTACCTCTGTTGATGAGAGCATTGCCT GGGGTAGATCCAAACGACTTTAGTAAGTGCATGATCACATTCCAATTTATAGGAACATTTTCTACTCTGG TGCCTTTAGTAGACTGTTCATCTGTACTGCAAGAAAGAAATGACCTCACAGAAATTGAAAAAGAACTTTG TTCGGCAACAGCTGAATTTGAGGATTTTGTGTTACAATTTATGGACAGATGCTTTGGACTAATAGAAAGT AGTACATTGGAGCAAACAAGGGAAGAGACAGAAACAGAGAAAATGACTCATTTGGAGAGTTTGGTTGAAT TAGGCTTATCTTCTACGTTTAGTACAATCCTCACACAATGTTCCAAAGACATATTTATGGTGGCCCTTCA GAAGGTCTTTAACTTTTCTGTTTCACATATATTTGAAACAAGAGTTGCAGGTCGCATGGTGGCAGACATG TGCCGAGCTGCTGTGAAGTGTTGCCCAGAAGAATCCCTGAAGCTGTTTGTTCCCCACTGCTATGGTGTTA TAACTCAGCTTACAATGAATGATGATGTATTGAATGAAGAAGAGCTAGACAAGGAGTTGCTGTGGAACCT TCAGCTTCTGTCTGAGATTACTCGTGTAGACGGGAAGAAGTTACTTCTGTATAGGGAGCAGCTTGTAAAG ATTCTCCAGCGAACCTTACACCTGACCTGTAAGCAGGGCTACACGCTGTCTTGCAACCTTCTGCATCATC TTCTTCGTTCTACCACACTAATCTACCCTACAGAGTACTGCAGTGTTCCGGGGGGCTTTAACAAGCCGCC TTCTGAATACTTCCCTGTCAAGGACTGGGGTAAGCCTGGAGATTTGTGGAATCTGGGGATCCAGTGGCAT GTTCCTTCTTCAGAAGAAGTCTCTTTTGCCTTCTATTTGTTAGACTCCTTTCTTCAGCCTGAGCTTATCA AACTCCAGTGTTGTGGAGATGGAGAACTTGAAATGTCTAGAGATGATATTCTACAGAGTCTGACTATAGT ACACAGCTGCTTGATTGGTTCAGGGAACCTTCTACCTCCATTGAAAGGAGAGGCAGTTACTAACTTGGTA CCAAGTATGGTCTCCTTAGAGGAAACAAAATTGTATACCGGACTGGAACATGACCTATCCAGAGAAAACT ACCGAGAAGTAATTGCTAGTGTCATAAGGAAACTTCTTAGCCACATACTTGATAATTCAGAAGATGATAC CAAATCACTGTTTCTCATCATAAAGATCATTGGAGACCTTCTGCACTTCCAAGGATCTCATAAACATGAA TTTGACTCTCGATGGAAAAGCTTTAACTTAGTAAAGAAATCAATGGAAAATCGGCTCCATGGAAAAAAGC AACATATCAGAGCATTACTAATTGACAGAGTGATGCTGCAGCATGAGTTGCGAACACTAACTGTTGAGGG TTGTGAGTACAAAAAAATACATCAAGATATGATCAGAGATCTTCTTCGATTATCAACAAGCTCATACAGT CAGGTCAGAAACAAAGCTCAGCAAACATTTTTTGCTGCTTTGGGGGCATATAACTTCTGTTGCAGAGATA TTATCCCCTTGGTTTTGGAATTCCTACGGCCTGATAGAAAGGATGTCACACAGCAGCAGTTCAAGGGCGC CTTATATTGTCTACTTGGAAATCACAGTGGTGTGTGCCTGGCAAACCTTCATGATTGGGACTGTATTGTG CAGACCTGGCCAGCACTTGTTTCTTCAGGGCTTAGCCAAGCAATGTCACTGGAAAAGCCATCAATAGTGA GACTATTTGATGACCTTGCTGAAAAGATTCATAGACAATATGAAACAATTGGCTTGGATTTTACAATTCC AAAGTCATGTGCTGCAATAGCAGAATTACTTCAACAATCAAAGAATCCTTCCATTAGCCAGACACTACTT AGCCCAGAAAAGATTAAAGAAGGACAGAAGCGTCAACAAGATAAGAATGCTGATGCCCTAAGGAACTATG AATGTTTGGTAAATACTTTACTAGATGGTGTGGAGCAAAGAAATCTGCCTTGGAAATTTGAGCATATAGG AATCGGGCTTCTGTCTCTACTCCTAAGGGATGACAGAGTGTTGCCTCTTCGTGCCATACGTTTTTTTGTT GAAAATCTCAACCATGATGCAATTGTTGTTCGAAAGATGGCTATCTCAGCTGTTGCTGGTATTCTCAAGC AGCTAAAGAGAACTCACAAAAAGCTGACTATCAACCCTTATGAAATCAGTGGATGTCCTAAACCTACCAA AATTCTTGCTGGTGATAGACCTGATAACCATTGGTTGCATTATGATAGTAAAAATATACCAAGAACAAAA AAAGAATGGGAGTCAAGTTGCTTTGTTGAAAAAACTCACTGGGGATACTACAACTGGCCAAAGAATATGG TTGTTTATGCTGGTGTGGAAGAACAGCCTAAACTTGGCAGAAGCAGGGAGGATATGATAGAGGCAGAACA GATTATATATGATCGTTTTTCTGATCCTAAGTTTGTTGAGCAGTTAATCACATTTCTATCTTTAGAAGAC AGAAAAGGAAGAGATAAGTTTAGTCCCCGGCGTTTCTGCCTCTTTAAGGGTATATTTAGAAATTTTGATG ATGCTTTTCTGCCTGTTCTGAAGCCCCATTTAGAACGTTTGGTTGCAGATTCTCATGAAAGTACCCAGAG ATGTGTTGCAGAAATTATAGCTGGTTTAATCAGAGGTTCTAAGCATTGGACGTTTGAGAAGGTGGAGAAG CTTTGGGAGCTCCTGTGCCCGCTGCTGAGAACGGCACTGTCCAACATGACAGTGGAGACATACAATGACT GGGGGACCTGTATAGCCACTTCCTGTGAAAGCAGAGATCCCCGGAAGCTTCACTGGCTTTTTGAGCTGCT CTTGGAGTCACCATTGAGTGGTGAAGGGGGGTCCTTTGTAGATGCATGCCGACTCTATGTGCTACAAGGT GGCCTTGCCCAGCAAGAGTGGAGAGTCCCTGAACTATTGCACAGACTTCTGAAGTACTTGGAACCAAAAC TCACCCAGGTTTACAAAAATGTCAGAGAAAGAATAGGAAGTGTGCTGACCTACATATTTATGATAGATGT TTCTTTGCCAAATACTGCTCCAACAACATCTCCTTGTATCCCTGAGTTTACTGCTCGCGTCCTAGAAAAA CTGAAACCACTCACAGATGTGGATGAAGAAATTCAGAACCATGTTATGGAAGAAAATGGCATTGGTGAAG AAGATGAACGAACTCAGGGCATTAAACTCTTAAAAACTATATTGAAATGGCTTATGGCAAGTGCAGGAAG ATCTTTTTCTACAGCAGTCAAAGAGCAACTTCAGCTTCTGCCTTTGTTCTTTAAGATCGCTCCAGTGGAA AATGACAATAGTTATGATGAACTGAAAAGAGATGCAAAGCTCTGTTTGTCACTAATGTCTCAGGGGTTGC TGTACCCTCAACAAGTGCCTTTGATACTTCAGGTGCTAAGCCAGACAGCAAGAAGCAGTTCTTGGCATGC AAGATATACAGTGCTGACCTACCTCCAGACCATGGTATTTTATAACCTCTTTATTTTCCTAAACAATGAA GATGCAGTTAAAGACATCAGATGGCTGATTATATGCCTTTTGGAGGATGAACAGTTAGAGGTTCGAGAAA TGGCTGCTACCACCTTAAGTGGTCTGTTACAGTGTAACTTCCTTACCATGGACAGTGCTATGCAGATTCA TTTTGAGCAACTTTGCAAAACAAAACTACCCAAGAAAAGAAAACGAGACCCTGGTTCTGTGGGAGATACC ATTCCTTCTGCAGAGTTGGTCAAACGTCATGCTGGTGTGCTTGGACTTGGTGCATGTGTTCTTTCTAGCC CTTATGATGTTCCTACCTGGATGCCCCAGCTCCTTATGAACCTCAGTGCACATTTGAATGATCCTCAGCC TATTGAGATGACTGTAAAGAAGACCTTATCCAATTTCCGAAGGACACATCATGACAACTGGCAAGAACAC AAGCAGCAGTTCACTGATGACCAGCTGCTTGTCCTCACTGACCTCCTTGTGTCACCATGCTATTATGCAT AG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_134013
Insert Size	5532 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_134013.3, NP_598774.2
RefSeq Size	6454 bp
RefSeq ORF	5532 bp
Locus ID	103554
UniProt ID	Q5SSW2
Gene Summary	Associated component of the proteasome that specifically recognizes acetylated histones and promotes ATP- and ubiquitin-independent degradation of core histones during spermatogenesis and DNA damage response. Recognizes and binds acetylated histones via its bromodomain-like (BRDL) region and activates the proteasome by opening the gated channel for substrate entry. Binds to the core proteasome via its C-terminus, which occupies the same binding sites as the proteasomal ATPases, opening the closed structure of the proteasome via an active gating mechanism. Component of the spermatoproteasome, a form of the proteasome specifically found in testis: binds to acetylated histones and promotes degradation of histones, thereby participating actively to the exchange of histones during spermatogenesis. Also involved in DNA damage response in somatic cells, by promoting degradation of histones following DNA double-strand breaks.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.