Kif20b (NM_183046) Mouse Tagged ORF Clone

CAT#: MG217235

  • TrueORF®

Kif20b (tGFP-tagged) - Mouse kinesin family member 20B (Kif20b), (10ug)

ORF Plasmid: DDK tGFP



  "NM_183046" in other vectors (2)

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Mouse Protein / Lysate Request

CNY 15,580.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms 33cex; B130024C23; C330014J10Rik; magoo; Mphosph1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG217235 representing NM_183046
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAATCTCACTTGAATCCAGATGGAGTGCCTAGACCGTCTTACGTTTTTAGTGCTGACCCAATTGCAA
GGCCTTTGGAAATCAATTTTGATGGTGTTAAGCTTGATCTCTCTCATGAGTTTTCCTTAGTCGCCTCAAA
CCCTGCGGCAAACAGTTTGGGATCTAAAAACTATCTTCAAGTTTGTCTTCGAATAAGACCATTTACACAG
TCAGAAAAAGAACATGAGGCTGAGGGCTGTGTGCAAGTTTTGGATTCACAGTCTGTTCTGCTGAAAGACC
CTCAAAGCATCCTTGGCCACTTAAGTGAGAAGAGCTCTGGACAGGTGGCACAGAAATTCAGTTTTTCTAA
GGTTTTTGGACCTGAAACTTCACAGAAAGAGTTCTTTCTGGGTTGCATTATGCAGCCAGTAAAGGACCTC
TTAGAAGGACACAGTCGACTGATCTTCACATATGGTCTGACCAATTCTGGAAAAACATATACATTTCAAG
GCACAGAAGAAAATATTGGTATTTTGCCTAGAACACTGAATGTATTGTTTGATAGTCTTCAGGAAAGACT
ATATACGAAGATGAGCTTCAAACCACACAGATGTAGAGAATACTTAAAATTATCATCTGATCAAGAGAAG
GAGGAAAGTGCTAACAAAAATACATTGCTGCGACAAATTAAGGAGGTTACCATACATAATGACAGTTATG
ACGTTCTTTGTGGACACTTAACAAACTCTTTGACTATCCCAGAATTTGAAGAATCCGTGAACAGTTGTGA
CCAGTCCAGTCTGAATGTGGATAATATAAAGTATTCTGTGTGGGTTTCTTTCTTTGAAATTTACAATGAA
AGTATTTACGATTTGTTTGTTCCTGTGTCCTCTAAGTTTCAAAAGAGAAAAATGTTACGACTTTCCCAAG
ATATAAAGGGCTATTCTTTTATAAAAGATCTGCAGTGGGTCCAGGTATCTGACTCTAAAGAAGCATATAG
ACTCTTAAAACTAGGAGTGAAGCATCAGAGCGTTGCTTTCACCAAACTGAACAATGCTTCTAGTAGAAGT
CACAGCATATTCACTATTAGAATATTACAGATAGAAGATTCTGAAATACCTCGTGTAACACGAGTTAGTG
AATTGTCTCTATGTGATCTTGCTGGTTCAGAACGAAGCATGAAGACACAAAACGAAGGTGAAAGGTTAAG
AGAGGCTGGGAATATCAACACTTCTTTATTGACTCTGGGAAAGTGTATCAATGTTTTGAAGAACAGTGAA
AAGTCAAAGGTTCAGCATGTGCCTTTCCGAGAAAGTAAACTGACCCACTATTTCCAAAGTTTTTTCACTG
GAAAAGGGAAAATATGCATGATCATCAATATTAGTCAGTCTTGCTCTGCCTATGATGAGACACTCAATGT
CCTGAAGTTCTCAACCACTGCGCAAAGGGTCTATGTTCCGGATACTTTAAGTTCTTCTCAAGAGAAATCT
TTTGCATCTAACAAATCTTTACAAGATGTATCATTAGACAGTAATTTGGACAATAAAATACTAAATGTAA
AAAGAAAAACTGTTTCATGGGAAAATAGTCTAGAAGATGTGCTTGAAAATGAAGATTTGGTTGAGGACTT
AGAAGAAAATGAAGAAACACAAAACATGGAAACGGAACTTACAGATGAAGATAGTGATAAGTCATTAGAG
GAATGCAGAGTTTCCACCTGCCACAAAAAGAACAAGGAACTGTTGGATTTAATAGAAAAGTTGAATAAAA
GACTAATAAATGAAAATAAAGAAAAATTAACATTGGAACTTAAAATCCGAGAAGAAGTTACACAGGAATT
CACTCAGTATTGGAGCCAACGGGAAGCCGATTTTAAGGAGACACTTCTTCATGAACGAGAGATATTAGAA
GAAAACGCTGAACGTCGCTTGGCAATCTTCAAAGATTTGGTTGGAAAATGTGACAGTCAAGATGAACCAA
CAAATAGAATTTGTGACATAGAACTTGAAACTGAAGAAGCTCATAATTATGTAGGAGTTGAAGATATTTT
TCATTCTCTTCAAGATGATGTCACTGATATTAAGAAACAGGCCGAACTTGCTCATTTGTATATTACATCT
CTTGTTGATCCCCAGGAAGCTATTGCCTGTTTGCAGCTAAAGTTTAATCAAGTTAAAGCAGAATTAGCTG
AAACTAAAGAAGAATTAATTAAAGCCCAAGAAGAGCTAAAAAACAGAGAGAGCAACTCTTTGGTTCAGGC
ACTCAAGACATCAAGTAAGGTTGATACATCTTTAACCAGCAATAAGTCAACTTGTAATGAAACAAGTGAA
ATGCCAAAGAACAGTAGAGCCCAGACACATTCAGAAAGGAAAAGATTAAATGAAGATGGACTTCAGCTTG
GTGAGCCACCAGCAAAGAAAGGGCTTATACTCGTTAGTCCACCCATCACCGAAGAGCAAAACAAAATGGG
AGAAATGCAACAGTCTGTTTCAGAAGTTGTGGAAGGCAATAGAGTTTTGAAGGAAAAGAATGAAGAGCTA
AAAAGACTTTTAACTATTGGTGAGAATGAACTCAGAAATGAAAAGGAGGAAAAAGCAGAATTAAATAAAC
AGGTTGTGAGTTTGCAGCAGCAACTTCGTTTCTTTGAAGAAAAGAACTCATCCCTGAGGGCAGATGTAGA
GCAAATCCAGGCCAGTTACAACAGTGCAGTTGCCGAGCTGCAAACACAGAAAGCCGTGAACCAGGAGCAG
AGGGACAGGATCCTGAAATTATCACAGGAGATGGAGACTGCTGCAAGAAGTATCGAGAGCAATGTTTCAC
AGATAAAACAAATGCAAACAAAAATAGATGAATTGCGGTCACTTGATTCACCTTCTCATATTTCAAAAAT
AGATTTACTTAATCTCCAGGATCTATCAAGTGGTGCTAAAGGGGATAATTGTCTGAACACATCACAGCAG
CTTCCAGGTGGTGACTTCTCAAGTACGTGGGTTAAAGAATATCACACTCAGGAGATCAGCAGGGAAAATT
CCTTCCACGCGAGTATTGAAGCCATCTGGGAAGAATGTAAAGAGATTGTGAAGGCTTCCTCCAAAAAGTC
TCATCAGATTCAGGGACTAGAAGAACAAATTGAAAAATTACAGGTGGAAGTAAAAGGATACAGGGAGGAG
AACAGTGACCTCCGAGCACAGGAGAGCCAGGGTAAGAATAGAGATCACCAGCTGAAGGAGAAAGAAAGTC
TCATCCAGCAGCTCAGAGAAGAACTGCAAGAAAAAAGTGTCAGTCTCCGTGTTCAAGTACAGCTTGTAGC
GGAAAGAGAGCAAGCTCTTTCAGAGCTTTCCCAGGATGTTACCTGCTACAAGGCAAAAATAAAGGACCTT
GAGGTGATAGTAGAGACCCAGAAAGATGAGTGTAAGCGCTTAGTTGAGTTAGAGCAAAGCATTTTAGAGA
AGGAATCTGCCATCTTAAAACTGGAAGCAAATCTAAAGGAATGTGAAGCAAAGCATCAGGATCACATCAG
AACCAACGATCTAAGTGCAAAGGAAGTCAAGTTCAGAGAAGAAGTCACACGGTTGGCGAATAATTTGCAT
GATACGAAACAGTTACTTCAGTCAAAGGAAGAAGAAAATGAAATTAGCAGACAAGAGACAGAAAAGTTGA
AAGAGGAGCTTGCTGCAAACTCTATTCTTACTCAGAATCTCAAAGCAGACCTTCAGAAGAAGGAAGAAGA
TTGTGCTGAGCTGAAAGAGAAGTTCATTGATGCCAAAAAGCAGATTGAGCAGGTACAGAGGGAGGTATCT
GTAATGCGTGATGAAGAGAAATTATTGAGGATTAAAATTAATGAACTGGAGAAAAAGAAAAACCAGTATT
CTCAGGACTTAGATATGAAACAACGAACCATTCAGCAACTTAAGGAGCAGTTAAGTAATCAGAAAATGGA
AGAAGCTGTACAACAGTATGAGAAAGTGTGCAAAGATCTCAGTGTTAAGGAGAAGCTGGTTGAAGACATG
CGACTGACGCTAGTGGAGCAGGAGCAGACTCAGGCAGAGCAGGACCGGGTGCTCGAGGCCAAGTCAGAGG
AAGCAGACTGGCTAGCCACAGAATTGGATAAATGGAAAGAAAAATTCAAAGATCTGGAAACCAGAAGTAA
TCAGAGGTTAAACACAGGAACCATGGATGACCTAGACGTGCTTACCAGGAAGTTCAGCAAGCTTCAGGAC
GAGCTGCAGGAATCTGAAGAGAAATATAAAGCTGATAGAAAGAAGTGGTTAGAAGAAAAAGCTGTCCTTA
CCACTCAAGCGAAAGAAGCAGAAAATGTACGAAACAGGGAGATGAGAAAATATGCTGACGACAGGGAGCG
CTGTTTAAAGCTGCAGAATGAAGTGGAAACACTGACAGCACAGCTGGCAGAGAAAAATAGTGAGCTTCAG
AAGTGGAGAGAAGAGCGTGACCAGCTGGTCACAGCTGTAGAGACACAGATGAAGGCTCTGCTCTCCAGCT
GCAAGCACAAGGATGAAGAAATCCAGGAACTAAGAAAGGCTGCAGCAAAGAGCACCGGGACAGAAAACCA
AACCATGAATCCCAAGCCTGAATATAATGATTCCGTTGATCTTGGTGGAGTTGAAACTGAACCTCAGTCA
ACAAGTTTGGAAATTTCTAGGAACACAGCAGAGGACGGATCTGTAGTTCTTGACTCTTGTGAAGTGTCAA
CAGAAAATGTACAAAGTACTCGATTTCCAAAACCTGAATTGGAGATTCAGTTTACACCTTTGCAGCCAAA
CAAAATGGCAGTGAAGCACCCTGGTTGTCCCACACCAGTCACAATTAAGATCCCCAAGGCACGGAAGAGG
AAGAGTGGTGAAGTGGAGGAGGACTTGGTGAAATGTGAAAATAAGAAGAATTCAACCCCCAGAAGTAATG
TGAAGTTTCCCGTTTCAGAGCATAGAAATTCCCCTGTCAAAAAGGAACAAAAGGTTTCCGTTGGTCCATC
ATCTAAGAAAACTTACTCCTTACGGAGCCAAGCATCTACAGTTAGTGCAAACATAGCCTCTAAAAAAAGA
GAGGGAACACTACAGAAATTTGGAGACTTCTTGCAGCATTCTCCAACAATTCTTCAATCAAAAGCAAAAA
AGATAATTGAAACAATGAGTTCTCCAAAGTTATCAACGGTAGAAGTAAGTAAAGAAAATGTGTCCCAGCC
AAAAAAAGCCAAACGGAAATTATACAGAAATGAAATTTCATCTCCCATCAACATATCTGGCCAAGTGATT
TTAATGGAACAGAAAGTAAAAGAAACTGATCATCAGATTCTCAAACGACGGCTTCGAACACGCACAGCCA
AA


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG217235 representing NM_183046
Red=Cloning site Green=Tags(s)

MESHLNPDGVPRPSYVFSADPIARPLEINFDGVKLDLSHEFSLVASNPAANSLGSKNYLQVCLRIRPFTQ
SEKEHEAEGCVQVLDSQSVLLKDPQSILGHLSEKSSGQVAQKFSFSKVFGPETSQKEFFLGCIMQPVKDL
LEGHSRLIFTYGLTNSGKTYTFQGTEENIGILPRTLNVLFDSLQERLYTKMSFKPHRCREYLKLSSDQEK
EESANKNTLLRQIKEVTIHNDSYDVLCGHLTNSLTIPEFEESVNSCDQSSLNVDNIKYSVWVSFFEIYNE
SIYDLFVPVSSKFQKRKMLRLSQDIKGYSFIKDLQWVQVSDSKEAYRLLKLGVKHQSVAFTKLNNASSRS
HSIFTIRILQIEDSEIPRVTRVSELSLCDLAGSERSMKTQNEGERLREAGNINTSLLTLGKCINVLKNSE
KSKVQHVPFRESKLTHYFQSFFTGKGKICMIINISQSCSAYDETLNVLKFSTTAQRVYVPDTLSSSQEKS
FASNKSLQDVSLDSNLDNKILNVKRKTVSWENSLEDVLENEDLVEDLEENEETQNMETELTDEDSDKSLE
ECRVSTCHKKNKELLDLIEKLNKRLINENKEKLTLELKIREEVTQEFTQYWSQREADFKETLLHEREILE
ENAERRLAIFKDLVGKCDSQDEPTNRICDIELETEEAHNYVGVEDIFHSLQDDVTDIKKQAELAHLYITS
LVDPQEAIACLQLKFNQVKAELAETKEELIKAQEELKNRESNSLVQALKTSSKVDTSLTSNKSTCNETSE
MPKNSRAQTHSERKRLNEDGLQLGEPPAKKGLILVSPPITEEQNKMGEMQQSVSEVVEGNRVLKEKNEEL
KRLLTIGENELRNEKEEKAELNKQVVSLQQQLRFFEEKNSSLRADVEQIQASYNSAVAELQTQKAVNQEQ
RDRILKLSQEMETAARSIESNVSQIKQMQTKIDELRSLDSPSHISKIDLLNLQDLSSGAKGDNCLNTSQQ
LPGGDFSSTWVKEYHTQEISRENSFHASIEAIWEECKEIVKASSKKSHQIQGLEEQIEKLQVEVKGYREE
NSDLRAQESQGKNRDHQLKEKESLIQQLREELQEKSVSLRVQVQLVAEREQALSELSQDVTCYKAKIKDL
EVIVETQKDECKRLVELEQSILEKESAILKLEANLKECEAKHQDHIRTNDLSAKEVKFREEVTRLANNLH
DTKQLLQSKEEENEISRQETEKLKEELAANSILTQNLKADLQKKEEDCAELKEKFIDAKKQIEQVQREVS
VMRDEEKLLRIKINELEKKKNQYSQDLDMKQRTIQQLKEQLSNQKMEEAVQQYEKVCKDLSVKEKLVEDM
RLTLVEQEQTQAEQDRVLEAKSEEADWLATELDKWKEKFKDLETRSNQRLNTGTMDDLDVLTRKFSKLQD
ELQESEEKYKADRKKWLEEKAVLTTQAKEAENVRNREMRKYADDRERCLKLQNEVETLTAQLAEKNSELQ
KWREERDQLVTAVETQMKALLSSCKHKDEEIQELRKAAAKSTGTENQTMNPKPEYNDSVDLGGVETEPQS
TSLEISRNTAEDGSVVLDSCEVSTENVQSTRFPKPELEIQFTPLQPNKMAVKHPGCPTPVTIKIPKARKR
KSGEVEEDLVKCENKKNSTPRSNVKFPVSEHRNSPVKKEQKVSVGPSSKKTYSLRSQASTVSANIASKKR
EGTLQKFGDFLQHSPTILQSKAKKIIETMSSPKLSTVEVSKENVSQPKKAKRKLYRNEISSPINISGQVI
LMEQKVKETDHQILKRRLRTRTAK

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_183046
ORF Size 5322 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_183046.2
RefSeq Size 5563 bp
RefSeq ORF 5325 bp
Locus ID 240641
UniProt ID Q80WE4
Gene Summary Plus-end-directed motor enzyme that is required for completion of cytokinesis (By similarity). Required for proper midbody organization and abscission in polarized cortical stem cells (PubMed:24173802). Plays a role in the regulation of neuronal polarization by mediating the transport of specific cargos. Participates in the mobilization of SHTN1 and in the accumulation of PIP3 in the growth cone of primary hippocampal neurons in a tubulin and actin-dependent manner (PubMed:23864681). In the developing telencephalon, cooperates with SHTN1 to promote both the transition from the multipolar to the bipolar stage and the radial migration of cortical neurons from the ventricular zone toward the superficial layer of the neocortex (PubMed:23864681). Involved in cerebral cortex growth (PubMed:24173802). Acts as an oncogene for promoting bladder cancer cells proliferation, apoptosis inhibition and carcinogenic progression (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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