Kif20b (NM_183046) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	33cex; B130024C23; C330014J10Rik; magoo; Mphosph1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224920 representing NM_183046 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAATCTCACTTGAATCCAGATGGAGTGCCTAGACCGTCTTACGTTTTTAGTGCTGACCCAATTGCAA GGCCTTTGGAAATCAATTTTGATGGTGTTAAGCTTGATCTCTCTCATGAGTTTTCCTTAGTCGCCTCAAA CCCTGCGGCAAACAGTTTGGGATCTAAAAACTATCTTCAAGTTTGTCTTCGAATAAGACCATTTACACAG TCAGAAAAAGAACATGAGGCTGAGGGCTGTGTGCAAGTTTTGGATTCACAGTCTGTTCTGCTGAAAGACC CTCAAAGCATCCTTGGCCACTTAAGTGAGAAGAGCTCTGGACAGGTGGCACAGAAATTCAGTTTTTCTAA GGTTTTTGGACCTGAAACTTCACAGAAAGAGTTCTTTCTGGGTTGCATTATGCAGCCAGTAAAGGACCTC TTAGAAGGACACAGTCGACTGATCTTCACATATGGTCTGACCAATTCTGGAAAAACATATACATTTCAAG GCACAGAAGAAAATATTGGTATTTTGCCTAGAACACTGAATGTATTGTTTGATAGTCTTCAGGAAAGACT ATATACGAAGATGAGCTTCAAACCACACAGATGTAGAGAATACTTAAAATTATCATCTGATCAAGAGAAG GAGGAAAGTGCTAACAAAAATACATTGCTGCGACAAATTAAGGAGGTTACCATACATAATGACAGTTATG ACGTTCTTTGTGGACACTTAACAAACTCTTTGACTATCCCAGAATTTGAAGAATCCGTGAACAGTTGTGA CCAGTCCAGTCTGAATGTGGATAATATAAAGTATTCTGTGTGGGTTTCTTTCTTTGAAATTTACAATGAA AGTATTTACGATTTGTTTGTTCCTGTGTCCTCTAAGTTTCAAAAGAGAAAAATGTTACGACTTTCCCAAG ATATAAAGGGCTATTCTTTTATAAAAGATCTGCAGTGGGTCCAGGTATCTGACTCTAAAGAAGCATATAG ACTCTTAAAACTAGGAGTGAAGCATCAGAGCGTTGCTTTCACCAAACTGAACAATGCTTCTAGTAGAAGT CACAGCATATTCACTATTAGAATATTACAGATAGAAGATTCTGAAATACCTCGTGTAACACGAGTTAGTG AATTGTCTCTATGTGATCTTGCTGGTTCAGAACGAAGCATGAAGACACAAAACGAAGGTGAAAGGTTAAG AGAGGCTGGGAATATCAACACTTCTTTATTGACTCTGGGAAAGTGTATCAATGTTTTGAAGAACAGTGAA AAGTCAAAGGTTCAGCATGTGCCTTTCCGAGAAAGTAAACTGACCCACTATTTCCAAAGTTTTTTCACTG GAAAAGGGAAAATATGCATGATCATCAATATTAGTCAGTCTTGCTCTGCCTATGATGAGACACTCAATGT CCTGAAGTTCTCAACCACTGCGCAAAGGGTCTATGTTCCGGATACTTTAAGTTCTTCTCAAGAGAAATCT TTTGCATCTAACAAATCTTTACAAGATGTATCATTAGACAGTAATTTGGACAATAAAATACTAAATGTAA AAAGAAAAACTGTTTCATGGGAAAATAGTCTAGAAGATGTGCTTGAAAATGAAGATTTGGTTGAGGACTT AGAAGAAAATGAAGAAACACAAAACATGGAAACGGAACTTACAGATGAAGATAGTGATAAGTCATTAGAG GAATGCAGAGTTTCCACCTGCCACAAAAAGAACAAGGAACTGTTGGATTTAATAGAAAAGTTGAATAAAA GACTAATAAATGAAAATAAAGAAAAATTAACATTGGAACTTAAAATCCGAGAAGAAGTTACACAGGAATT CACTCAGTATTGGAGCCAACGGGAAGCCGATTTTAAGGAGACACTTCTTCATGAACGAGAGATATTAGAA GAAAACGCTGAACGTCGCTTGGCAATCTTCAAAGATTTGGTTGGAAAATGTGACAGTCAAGATGAACCAA CAAATAGAATTTGTGACATAGAACTTGAAACTGAAGAAGCTCATAATTATGTAGGAGTTGAAGATATTTT TCATTCTCTTCAAGATGATGTCACTGATATTAAGAAACAGGCCGAACTTGCTCATTTGTATATTACATCT CTTGTTGATCCCCAGGAAGCTATTGCCTGTTTGCAGCTAAAGTTTAATCAAGTTAAAGCAGAATTAGCTG AAACTAAAGAAGAATTAATTAAAGCCCAAGAAGAGCTAAAAAACAGAGAGAGCAACTCTTTGGTTCAGGC ACTCAAGACATCAAGTAAGGTTGATACATCTTTAACCAGCAATAAGTCAACTTGTAATGAAACAAGTGAA ATGCCAAAGAACAGTAGAGCCCAGACACATTCAGAAAGGAAAAGATTAAATGAAGATGGACTTCAGCTTG GTGAGCCACCAGCAAAGAAAGGGCTTATACTCGTTAGTCCACCCATCACCGAAGAGCAAAACAAAATGGG AGAAATGCAACAGTCTGTTTCAGAAGTTGTGGAAGGCAATAGAGTTTTGAAGGAAAAGAATGAAGAGCTA AAAAGACTTTTAACTATTGGTGAGAATGAACTCAGAAATGAAAAGGAGGAAAAAGCAGAATTAAATAAAC AGGTTGTGAGTTTGCAGCAGCAACTTCGTTTCTTTGAAGAAAAGAACTCATCCCTGAGGGCAGATGTAGA GCAAATCCAGGCCAGTTACAACAGTGCAGTTGCCGAGCTGCAAACACAGAAAGCCGTGAACCAGGAGCAG AGGGACAGGATCCTGAAATTATCACAGGAGATGGAGACTGCTGCAAGAAGTATCGAGAGCAATGTTTCAC AGATAAAACAAATGCAAACAAAAATAGATGAATTGCGGTCACTTGATTCACCTTCTCATATTTCAAAAAT AGATTTACTTAATCTCCAGGATCTATCAAGTGGTGCTAAAGGGGATAATTGTCTGAACACATCACAGCAG CTTCCAGGTGGTGACTTCTCAAGTACGTGGGTTAAAGAATATCACACTCAGGAGATCAGCAGGGAAAATT CCTTCCACGCGAGTATTGAAGCCATCTGGGAAGAATGTAAAGAGATTGTGAAGGCTTCCTCCAAAAAGTC TCATCAGATTCAGGGACTAGAAGAACAAATTGAAAAATTACAGGTGGAAGTAAAAGGATACAGGGAGGAG AACAGTGACCTCCGAGCACAGGAGAGCCAGGGTAAGAATAGAGATCACCAGCTGAAGGAGAAAGAAAGTC TCATCCAGCAGCTCAGAGAAGAACTGCAAGAAAAAAGTGTCAGTCTCCGTGTTCAAGTACAGCTTGTAGC GGAAAGAGAGCAAGCTCTTTCAGAGCTTTCCCAGGATGTTACCTGCTACAAGGCAAAAATAAAGGACCTT GAGGTGATAGTAGAGACCCAGAAAGATGAGTGTAAGCGCTTAGTTGAGTTAGAGCAAAGCATTTTAGAGA AGGAATCTGCCATCTTAAAACTGGAAGCAAATCTAAAGGAATGTGAAGCAAAGCATCAGGATCACATCAG AACCAACGATCTAAGTGCAAAGGAAGTCAAGTTCAGAGAAGAAGTCACACGGTTGGCGAATAATTTGCAT GATACGAAACAGTTACTTCAGTCAAAGGAAGAAGAAAATGAAATTAGCAGACAAGAGACAGAAAAGTTGA AAGAGGAGCTTGCTGCAAACTCTATTCTTACTCAGAATCTCAAAGCAGACCTTCAGAAGAAGGAAGAAGA TTGTGCTGAGCTGAAAGAGAAGTTCATTGATGCCAAAAAGCAGATTGAGCAGGTACAGAGGGAGGTATCT GTAATGCGTGATGAAGAGAAATTATTGAGGATTAAAATTAATGAACTGGAGAAAAAGAAAAACCAGTATT CTCAGGACTTAGATATGAAACAACGAACCATTCAGCAACTTAAGGAGCAGTTAAGTAATCAGAAAATGGA AGAAGCTGTACAACAGTATGAGAAAGTGTGCAAAGATCTCAGTGTTAAGGAGAAGCTGGTTGAAGACATG CGACTGACGCTAGTGGAGCAGGAGCAGACTCAGGCAGAGCAGGACCGGGTGCTCGAGGCCAAGTCAGAGG AAGCAGACTGGCTAGCCACAGAATTGGATAAATGGAAAGAAAAATTCAAAGATCTGGAAACCAGAAGTAA TCAGAGGTTAAACACAGGAACCATGGATGACCTAGACGTGCTTACCAGGAAGTTCAGCAAGCTTCAGGAC GAGCTGCAGGAATCTGAAGAGAAATATAAAGCTGATAGAAAGAAGTGGTTAGAAGAAAAAGCTGTCCTTA CCACTCAAGCGAAAGAAGCAGAAAATGTACGAAACAGGGAGATGAGAAAATATGCTGACGACAGGGAGCG CTGTTTAAAGCTGCAGAATGAAGTGGAAACACTGACAGCACAGCTGGCAGAGAAAAATAGTGAGCTTCAG AAGTGGAGAGAAGAGCGTGACCAGCTGGTCACAGCTGTAGAGACACAGATGAAGGCTCTGCTCTCCAGCT GCAAGCACAAGGATGAAGAAATCCAGGAACTAAGAAAGGCTGCAGCAAAGAGCACCGGGACAGAAAACCA AACCATGAATCCCAAGCCTGAATATAATGATTCCGTTGATCTTGGTGGAGTTGAAACTGAACCTCAGTCA ACAAGTTTGGAAATTTCTAGGAACACAGCAGAGGACGGATCTGTAGTTCTTGACTCTTGTGAAGTGTCAA CAGAAAATGTACAAAGTACTCGATTTCCAAAACCTGAATTGGAGATTCAGTTTACACCTTTGCAGCCAAA CAAAATGGCAGTGAAGCACCCTGGTTGTCCCACACCAGTCACAATTAAGATCCCCAAGGCACGGAAGAGG AAGAGTGGTGAAGTGGAGGAGGACTTGGTGAAATGTGAAAATAAGAAGAATTCAACCCCCAGAAGTAATG TGAAGTTTCCCGTTTCAGAGCATAGAAATTCCCCTGTCAAAAAGGAACAAAAGGTTTCCGTTGGTCCATC ATCTAAGAAAACTTACTCCTTACGGAGCCAAGCATCTACAGTTAGTGCAAACATAGCCTCTAAAAAAAGA GAGGGAACACTACAGAAATTTGGAGACTTCTTGCAGCATTCTCCAACAATTCTTCAATCAAAAGCAAAAA AGATAATTGAAACAATGAGTTCTCCAAAGTTATCAACGGTAGAAGTAAGTAAAGAAAATGTGTCCCAGCC AAAAAAAGCCAAACGGAAATTATACAGAAATGAAATTTCATCTCCCATCAACATATCTGGCCAAGTGATT TTAATGGAACAGAAAGTAAAAGAAACTGATCATCAGATTCTCAAACGACGGCTTCGAACACGCACAGCCA AATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_183046
Insert Size	5325 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_183046.1, NP_898867.1
RefSeq Size	5563 bp
RefSeq ORF	5325 bp
Locus ID	240641
UniProt ID	Q80WE4
Gene Summary	Plus-end-directed motor enzyme that is required for completion of cytokinesis (By similarity). Required for proper midbody organization and abscission in polarized cortical stem cells (PubMed:24173802). Plays a role in the regulation of neuronal polarization by mediating the transport of specific cargos. Participates in the mobilization of SHTN1 and in the accumulation of PIP3 in the growth cone of primary hippocampal neurons in a tubulin and actin-dependent manner (PubMed:23864681). In the developing telencephalon, cooperates with SHTN1 to promote both the transition from the multipolar to the bipolar stage and the radial migration of cortical neurons from the ventricular zone toward the superficial layer of the neocortex (PubMed:23864681). Involved in cerebral cortex growth (PubMed:24173802). Acts as an oncogene for promoting bladder cancer cells proliferation, apoptosis inhibition and carcinogenic progression (By similarity).[UniProtKB/Swiss-Prot Function]

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