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Arhgap20 (NM_175535) Mouse Tagged ORF Clone
CAT＃: MG217166
- TrueORF®
Arhgap20 (tGFP-tagged) - Mouse Rho GTPase activating protein 20 (Arhgap20), (10ug)
ORF Plasmid: DDK
"NM_175535" in other vectors (2)
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CNY 10,550.00
货期*
3周
规格
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经常一起买 (4)
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 6530403F17Rik; A530023E23Rik; mKIAA1391 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG217166 representing NM_175535
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGCGATGTCTCCGCAGCAGGATGCTCTAGGGGCGCAGCCGGGGCGCTCCTCCTCGCTGACAGGCA TGTCTCGCATCGCGGGAGGCCCTGGCACCAAGAAGAAAATGAAAACACTCGCAGAAAGGAGGAGAAGCGC CCCGTCCCTCATCCTGGACAAAGCCTTACAGAAGCGGCCGAGCACCAGAGACAGCCATTCTGCCAGCATT GACACGTGTGCATTCCTCTCGTCCTTCATGTGCTCCAGTCGCACTCTGCTGATTGATGGTCCAGTGGAAC TTAAAAGAGGCCTGCAGAGGCAGGAGAGGCATCTTTTCCTATTCAACGATCTGTTTGTCTCAGCCAAAAT CAAATATAACAACAACTTTAAGATAAAGAATAAAATAAGACTAACTGACATGTGGACAGCTAGCTGTGTG GAGGAAGTGGGAGAAGGCAACATGAATGCTCAGAAGTCCTTTGTCCTGGGCTGGCCCACCGTGAACTTTG TGGCTACTTTCAGTTCTCCAGAACAAAAGGACAAATGGCTGTCTCTCCTTCAGAGATACATCGCTCTAGA AAAGGAGAAAGACTACCCGAAGAGCATCCCCCTCAAAATCTTTGCCAAGGACATTGGAAACTGTGCCTAT TTTAAAACTATAACAGTGATGAATTCAGACACAGCGAGTGAAGTTATCAACATGTCACTGCAAATGTTAG GAATAACTGGCTCTGAGAGAGATTACCAACTGTGGGTAAATTCTGGAAAAGAAGCGGCACCGTACCCACT CATTGGACACGAGTATCCTTATGGAATTAAAATGAGCCACCTCCGAGACACTGCCCTTCTGACGCAGGGA TCAAGAGACTCTGCCAGCCCATCCCAGCTCCAAGAGCCTTTCCTCATGGAGCAGCTGCCCCGCGAGATGC AATGCCAGTTCATCCTGAAACCTACCCGCCTGGCGACAGCCCAGCAACTGAGCGATTCCAGCCAGAAGAC ATTTAAAAGGAGGCGCTCTATCATCAACTGGGCTTTTTGGCGGGGTTCTAGCACACACCTGGACAACCTG CCCATGTCACCAACATCCCCTATGCCAGGACAGCTCTTTGGAGTTTCTTTACCAGATCTTTGTGAGAACG ACAATCTGCCAAAGCCTATCTTGGATATGCTTTCCTTCCTTAACCAAAAAGGCCCCCTCACAAAAGGCAT CTTCAGGCAGTCGGCCAACATGAAATCTTGCAGAGAGCTAAAGGAGAAACTGAATTCTGGAATCGAAGTC CACCTTGACTGTGAATCCATCTTTGTGATAGCATCTGTGTTGAAGGATTTTCTGAGAAATATCCCAGAAA GTATCTTTTCATCAGATCTATATGATCACTGGGTCTGTGTAATGGATCAAGGAAATGATGAAGAAAAAAT AAATATAATTCAAAGGCTATTAGATCAGCTTCCCAGAGCCAATGTTGTTTTCCTAAGGTATCTGTTTGGG GTATTACACAACATTGAACAACATTCCTTGTCTAATCAGATGACTGCATTTAACTTAGCAGTGTGTATCG CTCCAAGCATTCTTTGGCCTCCTGCTTCCTCCAGTCCAGAGCTAGAAAATGAATTTACAAAAAAGGTTTC TCTTCTTATACAATTTCTGATTGAAAACTGCTGTAGGGTATTTGGAGAAGAAATCGCTTCTCTCTTGGGG GAGCTTTCGGAGAGAAGCGACAGAGAACACACCCCAGATATAACTTGCTTCCAAATGAACGACTCCTCCT ATGACAGCTTGGAAAACGAGCTCAATGAGGAAGCTGATGCTCCATGCAGTGACCTGGTCAAAAAGCTTGG CCAGGGTAGCAGGAGCATGGACTCTGTCTTAACCCTCAGCGACTATGACCTTGAACAGCCTGAGGTGGAA GGCCTCTTAACCCTGAGCAACTTTGACTTAGATCAGTCTAAAGAGGAGCACATTCCGATAAAGCCACCTC TGGAGCCCAAGCCAGTGAATGTTTTCGTAGGCTACAGGAAGGTTTCACTAGGGGAGCACGCTAGGGCCCC AGCTGGCCCAGGCACACTCAGCTGCCTGCCTGTGGCTGCAGCAGATGCCCCGAAAGTCCTGCGGCGGCAC CGGCGCTCCTCAGAACCCAGCATTGACTATCTAGACACAAAGCTTTCCTATCTCCGGGAGTTTTACCAGA AAAAGCTGCGCAAGTCCAGCTGCGATGCTGTCCTCTCTAGAAAAGATGAAGATTACCTGAAGCAGACCCA GCCTCAGAAGAAAGGGGATAAGGTGTGTCTTAAACAGAGTTCAGTGACAGGCACTGACGTCAGCAAGAGA AACACAGCTAATGAAAACATTAAGAAGAAAAGCTTGTCTGGTCATGAAGGAACTCAAGTGACACTTTTTA CTAAGTCCAAGCCAGTACCCATCTCTGTGGCATCTTACAGCCACGGGTCCTCCCAGGATCATCCCAGGAA GCAAGCCTTTGATGCAGACCCATGCCGGTTCTCCCCACCACACCTAACAGATGCCCAAAAGAGCTCAAGG GTGCAACATCGGCGCTGTTCAGAACCCAGCATAGATGACCAGAACTACAAGCTGTCCTATCTCAGGGGGA TCTATTCAATGAAACAAAATAAAGCCAGCTGTGAGGCTGGCCTCTTGCATGGGGAGGACGACTATCTCAG AAGGCATAAGTCTTTGCAAATCGAGGGCCAAAAGCTTATTAATCAGAGTTTGGTCATGGGCATTGAGGTG GGCAAGAGCAGCAGCTCTCACCAAAGCACTGAGAAGGTTTTGCCCCCAAGACTAAACCTTTGCCCAAGGG CAAGCTATTCAAGCTTATCTTCTCCAGGCTCGTCCCCATCCGGCTCCTCTGTAAGCTCCCAGGACAGCGC TTTCTCTCAGATCTCTGAACACTCCGTGTTTACGCCCACCGAAACCTCCTCACCAATAGATTGCACTTTC CAGACACAAAGAAAACAAGAAGAGCTGTCTTCAGACTTTGACAGTCCCAGCCGCCTTTCCGGAATGCCCG GTCCCTCCATGGGTCAGGCCAGCAGCCACCTAGCTTATTTAAGAAAGGGTACCACGGAGCAGCCATCACA AATGCATTCTGTAACCCTTCACCCCAGTGCGTGGTTGAGAAGTGGCTTGGTCACTTTAAAAAACTGGTCC CTCAAAAAGAAAACAAAAGCAGCCAGACCAGAAGACAGGAAAGTCTGTTCCCTGAAAGAGCCTCTGGAGT TGCCGTCATGTGCTTCCGGTACCCCAGAGGCTGACTCTCTACAAGAGAGTCAGGATGACTTACAGGGAGA TGAAGGACCTGGGCAAACAGCCTGTGGATTCAGTTCTTATGCCTGTCAGGACTCAGAGCAACATGCCGGC TCTCCATTCCACCTGGCAGAAAGCAGACTCAAGCCTTGTATGAAGTTATATAAGGGAGAAGAGAGTGGAG GGCAGTACCCTTGTGATAACCCTTGGGAAGGAGCCTCTTCAAGTCTGGAGACCACGGAGGATACAGCCAA CCCAGGTGCAGAACCTACAACATTTGCGATGACGGGGACAGATATT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG217166 representing NM_175535
Red=Cloning site Green=Tags(s) MEAMSPQQDALGAQPGRSSSLTGMSRIAGGPGTKKKMKTLAERRRSAPSLILDKALQKRPSTRDSHSASI DTCAFLSSFMCSSRTLLIDGPVELKRGLQRQERHLFLFNDLFVSAKIKYNNNFKIKNKIRLTDMWTASCV EEVGEGNMNAQKSFVLGWPTVNFVATFSSPEQKDKWLSLLQRYIALEKEKDYPKSIPLKIFAKDIGNCAY FKTITVMNSDTASEVINMSLQMLGITGSERDYQLWVNSGKEAAPYPLIGHEYPYGIKMSHLRDTALLTQG SRDSASPSQLQEPFLMEQLPREMQCQFILKPTRLATAQQLSDSSQKTFKRRRSIINWAFWRGSSTHLDNL PMSPTSPMPGQLFGVSLPDLCENDNLPKPILDMLSFLNQKGPLTKGIFRQSANMKSCRELKEKLNSGIEV HLDCESIFVIASVLKDFLRNIPESIFSSDLYDHWVCVMDQGNDEEKINIIQRLLDQLPRANVVFLRYLFG VLHNIEQHSLSNQMTAFNLAVCIAPSILWPPASSSPELENEFTKKVSLLIQFLIENCCRVFGEEIASLLG ELSERSDREHTPDITCFQMNDSSYDSLENELNEEADAPCSDLVKKLGQGSRSMDSVLTLSDYDLEQPEVE GLLTLSNFDLDQSKEEHIPIKPPLEPKPVNVFVGYRKVSLGEHARAPAGPGTLSCLPVAAADAPKVLRRH RRSSEPSIDYLDTKLSYLREFYQKKLRKSSCDAVLSRKDEDYLKQTQPQKKGDKVCLKQSSVTGTDVSKR NTANENIKKKSLSGHEGTQVTLFTKSKPVPISVASYSHGSSQDHPRKQAFDADPCRFSPPHLTDAQKSSR VQHRRCSEPSIDDQNYKLSYLRGIYSMKQNKASCEAGLLHGEDDYLRRHKSLQIEGQKLINQSLVMGIEV GKSSSSHQSTEKVLPPRLNLCPRASYSSLSSPGSSPSGSSVSSQDSAFSQISEHSVFTPTETSSPIDCTF QTQRKQEELSSDFDSPSRLSGMPGPSMGQASSHLAYLRKGTTEQPSQMHSVTLHPSAWLRSGLVTLKNWS LKKKTKAARPEDRKVCSLKEPLELPSCASGTPEADSLQESQDDLQGDEGPGQTACGFSSYACQDSEQHAG SPFHLAESRLKPCMKLYKGEESGGQYPCDNPWEGASSSLETTEDTANPGAEPTTFAMTGTDI TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_175535 |
| ORF Size | 3546 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_175535.4 |
| RefSeq Size | 6365 bp |
| RefSeq ORF | 3549 bp |
| Locus ID | 244867 |
| UniProt ID | Q6IFT4 |
| Gene Summary | GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
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