Arhgap20 (NM_175535) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	6530403F17Rik; A530023E23Rik; mKIAA1391
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC223868 representing NM_175535 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGCGATGTCTCCGCAGCAGGATGCTCTAGGGGCGCAGCCGGGGCGCTCCTCCTCGCTGACAGGCA TGTCTCGCATCGCGGGAGGCCCTGGCACCAAGAAGAAAATGAAAACACTCGCAGAAAGGAGGAGAAGCGC CCCGTCCCTCATCCTGGACAAAGCCTTACAGAAGCGGCCGAGCACCAGAGACAGCCATTCTGCCAGCATT GACACGTGTGCATTCCTCTCGTCCTTCATGTGCTCCAGTCGCACTCTGCTGATTGATGGTCCAGTGGAAC TTAAAAGAGGCCTGCAGAGGCAGGAGAGGCATCTTTTCCTATTCAACGATCTGTTTGTCTCAGCCAAAAT CAAATATAACAACAACTTTAAGATAAAGAATAAAATAAGACTAACTGACATGTGGACAGCTAGCTGTGTG GAGGAAGTGGGAGAAGGCAACATGAATGCTCAGAAGTCCTTTGTCCTGGGCTGGCCCACCGTGAACTTTG TGGCTACTTTCAGTTCTCCAGAACAAAAGGACAAATGGCTGTCTCTCCTTCAGAGATACATCGCTCTAGA AAAGGAGAAAGACTACCCGAAGAGCATCCCCCTCAAAATCTTTGCCAAGGACATTGGAAACTGTGCCTAT TTTAAAACTATAACAGTGATGAATTCAGACACAGCGAGTGAAGTTATCAACATGTCACTGCAAATGTTAG GAATAACTGGCTCTGAGAGAGATTACCAACTGTGGGTAAATTCTGGAAAAGAAGCGGCACCGTACCCACT CATTGGACACGAGTATCCTTATGGAATTAAAATGAGCCACCTCCGAGACACTGCCCTTCTGACGCAGGGA TCAAGAGACTCTGCCAGCCCATCCCAGCTCCAAGAGCCTTTCCTCATGGAGCAGCTGCCCCGCGAGATGC AATGCCAGTTCATCCTGAAACCTACCCGCCTGGCGACAGCCCAGCAACTGAGCGATTCCAGCCAGAAGAC ATTTAAAAGGAGGCGCTCTATCATCAACTGGGCTTTTTGGCGGGGTTCTAGCACACACCTGGACAACCTG CCCATGTCACCAACATCCCCTATGCCAGGACAGCTCTTTGGAGTTTCTTTACCAGATCTTTGTGAGAACG ACAATCTGCCAAAGCCTATCTTGGATATGCTTTCCTTCCTTAACCAAAAAGGCCCCCTCACAAAAGGCAT CTTCAGGCAGTCGGCCAACATGAAATCTTGCAGAGAGCTAAAGGAGAAACTGAATTCTGGAATCGAAGTC CACCTTGACTGTGAATCCATCTTTGTGATAGCATCTGTGTTGAAGGATTTTCTGAGAAATATCCCAGAAA GTATCTTTTCATCAGATCTATATGATCACTGGGTCTGTGTAATGGATCAAGGAAATGATGAAGAAAAAAT AAATATAATTCAAAGGCTATTAGATCAGCTTCCCAGAGCCAATGTTGTTTTCCTAAGGTATCTGTTTGGG GTATTACACAACATTGAACAACATTCCTTGTCTAATCAGATGACTGCATTTAACTTAGCAGTGTGTATCG CTCCAAGCATTCTTTGGCCTCCTGCTTCCTCCAGTCCAGAGCTAGAAAATGAATTTACAAAAAAGGTTTC TCTTCTTATACAATTTCTGATTGAAAACTGCTGTAGGGTATTTGGAGAAGAAATCGCTTCTCTCTTGGGG GAGCTTTCGGAGAGAAGCGACAGAGAACACACCCCAGATATAACTTGCTTCCAAATGAACGACTCCTCCT ATGACAGCTTGGAAAACGAGCTCAATGAGGAAGCTGATGCTCCATGCAGTGACCTGGTCAAAAAGCTTGG CCAGGGTAGCAGGAGCATGGACTCTGTCTTAACCCTCAGCGACTATGACCTTGAACAGCCTGAGGTGGAA GGCCTCTTAACCCTGAGCAACTTTGACTTAGATCAGTCTAAAGAGGAGCACATTCCGATAAAGCCACCTC TGGAGCCCAAGCCAGTGAATGTTTTCGTAGGCTACAGGAAGGTTTCACTAGGGGAGCACGCTAGGGCCCC AGCTGGCCCAGGCACACTCAGCTGCCTGCCTGTGGCTGCAGCAGATGCCCCGAAAGTCCTGCGGCGGCAC CGGCGCTCCTCAGAACCCAGCATTGACTATCTAGACACAAAGCTTTCCTATCTCCGGGAGTTTTACCAGA AAAAGCTGCGCAAGTCCAGCTGCGATGCTGTCCTCTCTAGAAAAGATGAAGATTACCTGAAGCAGACCCA GCCTCAGAAGAAAGGGGATAAGGTGTGTCTTAAACAGAGTTCAGTGACAGGCACTGACGTCAGCAAGAGA AACACAGCTAATGAAAACATTAAGAAGAAAAGCTTGTCTGGTCATGAAGGAACTCAAGTGACACTTTTTA CTAAGTCCAAGCCAGTACCCATCTCTGTGGCATCTTACAGCCACGGGTCCTCCCAGGATCATCCCAGGAA GCAAGCCTTTGATGCAGACCCATGCCGGTTCTCCCCACCACACCTAACAGATGCCCAAAAGAGCTCAAGG GTGCAACATCGGCGCTGTTCAGAACCCAGCATAGATGACCAGAACTACAAGCTGTCCTATCTCAGGGGGA TCTATTCAATGAAACAAAATAAAGCCAGCTGTGAGGCTGGCCTCTTGCATGGGGAGGACGACTATCTCAG AAGGCATAAGTCTTTGCAAATCGAGGGCCAAAAGCTTATTAATCAGAGTTTGGTCATGGGCATTGAGGTG GGCAAGAGCAGCAGCTCTCACCAAAGCACTGAGAAGGTTTTGCCCCCAAGACTAAACCTTTGCCCAAGGG CAAGCTATTCAAGCTTATCTTCTCCAGGCTCGTCCCCATCCGGCTCCTCTGTAAGCTCCCAGGACAGCGC TTTCTCTCAGATCTCTGAACACTCCGTGTTTACGCCCACCGAAACCTCCTCACCAATAGATTGCACTTTC CAGACACAAAGAAAACAAGAAGAGCTGTCTTCAGACTTTGACAGTCCCAGCCGCCTTTCCGGAATGCCCG GTCCCTCCATGGGTCAGGCCAGCAGCCACCTAGCTTATTTAAGAAAGGGTACCACGGAGCAGCCATCACA AATGCATTCTGTAACCCTTCACCCCAGTGCGTGGTTGAGAAGTGGCTTGGTCACTTTAAAAAACTGGTCC CTCAAAAAGAAAACAAAAGCAGCCAGACCAGAAGACAGGAAAGTCTGTTCCCTGAAAGAGCCTCTGGAGT TGCCGTCATGTGCTTCCGGTACCCCAGAGGCTGACTCTCTACAAGAGAGTCAGGATGACTTACAGGGAGA TGAAGGACCTGGGCAAACAGCCTGTGGATTCAGTTCTTATGCCTGTCAGGACTCAGAGCAACATGCCGGC TCTCCATTCCACCTGGCAGAAAGCAGACTCAAGCCTTGTATGAAGTTATATAAGGGAGAAGAGAGTGGAG GGCAGTACCCTTGTGATAACCCTTGGGAAGGAGCCTCTTCAAGTCTGGAGACCACGGAGGATACAGCCAA CCCAGGTGCAGAACCTACAACATTTGCGATGACGGGGACAGATATTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_175535
Insert Size	3549 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_175535.3, NP_780744.2
RefSeq Size	6365 bp
RefSeq ORF	3549 bp
Locus ID	244867
UniProt ID	Q6IFT4
Gene Summary	GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state.[UniProtKB/Swiss-Prot Function]

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