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Cand1 (NM_027994) Mouse Tagged ORF Clone
CAT＃: MG216867
- TrueORF®
Cand1 (tGFP-tagged) - Mouse cullin associated and neddylation disassociated 1 (Cand1), (10ug)
ORF Plasmid: DDK
"NM_027994" in other vectors (4)
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CNY 13,490.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 2310038O07Rik; 6330512O03Rik; AI195005; AI846556; D10Ertd516e; mKIAA0829; Tp120a |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG216867 representing NM_027994
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGAGCGCTTCGTACCACATCTCCAACTTGCTGGAGAAAATGACATCCAGCGACAAGGACTTCAGGT TCATGGCGACCAACGACCTGATGACAGAGCTGCAGAAAGACTCTATCAAGCTGGACGATGACAGCGAGAG GAAAGTTGTGAAGATGATTCTCAGGTTGCTGGAGGATAAAAATGGCGAAGTGCAGAACTTAGCTGTCAAA TGCCTTGGCCCTTTGGTGAGTAAAGTGAAAGAGTACCAAGTGGAGACGATCGTGGACACCCTCTGCACCA ACATGCTTTCTGACAAGGAGCAGCTGCGAGACATTTCCAGCATCGGCCTGAAAACAGTAATTGGAGAACT TCCGCCAGCTTCCAGTGGCTCTGCATTAGCTGCTAATGTATGTAAAAAGATTACTGGACGCCTTACCAGT GCAATAGCAAAGCAGGAAGATGTTTCTGTTCAGCTCGAAGCCTTGGATATCATGGCTGACATGTTGAGCA GGCAAGGAGGACTGCTTGTTAATTTCCACCCTTCAATTCTGACCTGTCTGCTTCCCCAGCTGACCAGCCC TAGACTTGCAGTGAGGAAAAGAACAATTATTGCTCTTGGCCACCTAGTTATGAGCTGCGGAAATATAGTT TTTGTAGACCTTATTGAACATCTGTTGTCAGAGTTGTCCAAAAATGACTCCATGTCAACAACAAGGACCT ACATACAGTGTATTGCTGCTATTAGTAGGCAAGCTGGCCATAGAATAGGTGAATACCTTGAAAAGATAAT TCCTTTGGTGGTAAAATTTTGTAATGTAGATGATGATGAATTAAGAGAATACTGCATTCAAGCTTTTGAA TCATTTGTGAGAAGATGCCCTAAAGAAGTTTATCCTCATGTTTCTACCATTATAAACATTTGTCTAAAAT ACCTTACCTATGATCCAAATTACAATTACGATGATGAAGATGAAGATGAGAATGCTATGGATGCTGACGG TGGGGACGACGATGACCAAGGGAGTGACGATGAATATAGTGACGATGACGACATGAGTTGGAAAGTGAGA CGCGCGGCTGCTAAGTGCCTGGACGCTGTAGTTAGCACACGGCATGAGATGCTCCCGGAGTTCTACAAGA CTGTCTCTCCTGCGCTGATAGCCAGATTTAAAGAGCGGGAAGAAAATGTAAAGGCAGATGTTTTTCATGC ATACCTGTCTCTTCTGAAACAAACTCGTCCAGTGCAAAGTTGGCTGTGTGACCCTGACGCAATGGAGCAG GGAGACACGCCCCTAACAATGCTGCAGAGTCAGGTTCCCAACATTGTTAAAGCCCTGCACAAGCAGATGA AAGAGAAGAGTGTGAAGACCCGACAGTGTTGCTTTAACATGTTAACTGAGCTGGTAAATGTGCTGCCGGG AGCACTAACCCAGCACATTCCTGTACTGGTACCAGGAATCATTTTCTCACTGAATGATAAGTCCAGCTCA TCAAACCTGAAGATTGATGCGCTGTCGTGTCTGTATGTCATCCTCTGTAACCACTCTCCTCAAGTTTTCC ATCCTCATGTTCAGGCTCTGGTCCCTCCGGTGGTGGCCTGTGTGGGAGACCCGTTTTACAAGATCACTTC AGAAGCCCTTCTTGTCACTCAGCAGCTTGTCAAAGTGATCCGTCCTCTAGACCAGCCCTCTTCGTTCGAT GCAACGCCTTACATCAAAGATCTCTTCACCTGCACAATTAAGCGCTTAAAAGCAGCTGACATTGATCAAG AAGTCAAGGAAAGGGCTATTTCCTGTATGGGACAGATTATTTGCAATCTTGGAGACAATTTGGGTCCTGA TTTATCAAATACCCTACAGATTTTCTTGGAGAGACTCAAGAATGAGATTACCCGGCTAACGACCGTCAAA GCGCTGACCCTGATTGCTGGGTCACCTTTGAAGATAGATCTGAGGCCTGTGCTGGGAGAGGGAGTCCCCA TCCTTGCTTCATTCCTCAGGAAAAATCAGAGAGCTTTGAAACTGGGGACCCTCTCTGCCCTGGATATTCT CATTAAGAACTACAGTGACAGCCTGACGGCCGCCATGATTGACGCAGTTCTGGATGAGCTCCCCCCCCTC ATCAGCGAGAGCGATATGCACGTGTCGCAGATGGCTATCAGCTTCCTCACCACCCTGGCCAAGGTCTATC CCTCCTCCCTGTCCAAGATAAGCGGATCTATCCTCAATGAACTGATTGGACTTGTGAGATCGCCTCTACT GCAGGGAGGAGCGCTCAGTGCCATGCTAGACTTTTTCCAAGCTCTGGTTGTCACTGGAACAAACAATCTA GGGTACATGGACTTGCTGCGCATGCTAACGGGTCCAGTTTATTCTCAGAGCACTGCTCTCACTCATAAGC AGTCTTATTATTCCATTGCCAAATGTGTAGCTGCCCTTACTCGAGCTTGCCCTAAAGAGGGACCCGCCGT AGTAGGTCAGTTTATTCAAGATGTCAAGAACTCAAGGTCTACAGATTCCATTCGTCTCTTAGCACTCCTT TCTCTTGGAGAAGTTGGACACCATATTGACTTAAGTGGGCAGTTGGAATTAAAATCTGTAATATTAGAGG CTTTCTCATCCCCTAGTGAAGAAGTCAAATCAGCTGCGTCCTATGCATTAGGCAGCATTAGTGTAGGCAA CCTTCCTGAGTATCTGCCCTTTGTACTGCAAGAAATAACCAGCCAGCCCAAAAGGCAGTATCTTCTGCTT CACTCTTTGAAGGAGATCATTAGCTCTGCATCGGTGGCAGGCCTTAAGCCATATGTTGAGAACATCTGGG CCTTGCTGCTAAAGCACTGTGAGTGTGCGGAGGAAGGCACCAGGAATGTCGTGGCTGAATGTCTAGGGAA GCTCACTCTTATTGATCCTGAAACTCTCCTCCCACGGCTTAAAGGGTATTTGATATCAGGGTCATCATAT GCCAGGAGCTCGGTGGTTACAGCTGTGAAGTTCACGATTTCTGATCATCCTCAGCCTATTGATCCACTGT TGAAGAACTGCATAGGTGACTTTCTAAAAACTTTGGAAGACCCAGATTTGAACGTAAGAAGAGTGGCCTT GGTCACATTCAATTCTGCGGCCCATAACAAGCCATCACTGATACGGGACCTTCTGGACTCAGTTCTTCCA CATCTTTACAATGAGACAAAAGTTAGGAAGGAACTTATAAGAGAGGTAGAAATGGGTCCATTTAAACACA CGGTTGATGACGGTCTAGACATTAGAAAGGCAGCTTTTGAGTGTATGTATACACTTCTAGACAGCTGTCT TGATAGACTGGATATCTTTGAATTTCTAAATCATGTTGAAGATGGTTTGAAGGACCATTATGATATTAAG ATGCTAACATTTTTAATGTTGGTACGACTGTCTACCCTCTGTCCAAGTGCAGTACTACAAAGGCTGGACC GACTTGTTGAGCCACTGCGTGCTACGTGTACAACTAAGGTAAAGGCAAACTCTGTAAAGCAGGAGTTTGA AAAGCAAGATGAGCTAAAGCGGTCTGCCATGAGGGCAGTGGCGGCTTTGTTAACCATTCCAGAGGCAGAG AAGAGCCCTCTTATGAGTGAATTCCAGTCACAGATCAGCTCCAACCCGGAGCTGGCAGCCATCTTTGAAA GTATCCAGAAAGATTCTTCGTCCACCAACTTGGAATCAATGGACACGAGT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG216867 representing NM_027994
Red=Cloning site Green=Tags(s) MASASYHISNLLEKMTSSDKDFRFMATNDLMTELQKDSIKLDDDSERKVVKMILRLLEDKNGEVQNLAVK CLGPLVSKVKEYQVETIVDTLCTNMLSDKEQLRDISSIGLKTVIGELPPASSGSALAANVCKKITGRLTS AIAKQEDVSVQLEALDIMADMLSRQGGLLVNFHPSILTCLLPQLTSPRLAVRKRTIIALGHLVMSCGNIV FVDLIEHLLSELSKNDSMSTTRTYIQCIAAISRQAGHRIGEYLEKIIPLVVKFCNVDDDELREYCIQAFE SFVRRCPKEVYPHVSTIINICLKYLTYDPNYNYDDEDEDENAMDADGGDDDDQGSDDEYSDDDDMSWKVR RAAAKCLDAVVSTRHEMLPEFYKTVSPALIARFKEREENVKADVFHAYLSLLKQTRPVQSWLCDPDAMEQ GDTPLTMLQSQVPNIVKALHKQMKEKSVKTRQCCFNMLTELVNVLPGALTQHIPVLVPGIIFSLNDKSSS SNLKIDALSCLYVILCNHSPQVFHPHVQALVPPVVACVGDPFYKITSEALLVTQQLVKVIRPLDQPSSFD ATPYIKDLFTCTIKRLKAADIDQEVKERAISCMGQIICNLGDNLGPDLSNTLQIFLERLKNEITRLTTVK ALTLIAGSPLKIDLRPVLGEGVPILASFLRKNQRALKLGTLSALDILIKNYSDSLTAAMIDAVLDELPPL ISESDMHVSQMAISFLTTLAKVYPSSLSKISGSILNELIGLVRSPLLQGGALSAMLDFFQALVVTGTNNL GYMDLLRMLTGPVYSQSTALTHKQSYYSIAKCVAALTRACPKEGPAVVGQFIQDVKNSRSTDSIRLLALL SLGEVGHHIDLSGQLELKSVILEAFSSPSEEVKSAASYALGSISVGNLPEYLPFVLQEITSQPKRQYLLL HSLKEIISSASVAGLKPYVENIWALLLKHCECAEEGTRNVVAECLGKLTLIDPETLLPRLKGYLISGSSY ARSSVVTAVKFTISDHPQPIDPLLKNCIGDFLKTLEDPDLNVRRVALVTFNSAAHNKPSLIRDLLDSVLP HLYNETKVRKELIREVEMGPFKHTVDDGLDIRKAAFECMYTLLDSCLDRLDIFEFLNHVEDGLKDHYDIK MLTFLMLVRLSTLCPSAVLQRLDRLVEPLRATCTTKVKANSVKQEFEKQDELKRSAMRAVAALLTIPEAE KSPLMSEFQSQISSNPELAAIFESIQKDSSSTNLESMDTS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_027994 |
| ORF Size | 3690 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_027994.1, NP_082270.1 |
| RefSeq Size | 8209 bp |
| RefSeq ORF | 3693 bp |
| Locus ID | 71902 |
| UniProt ID | Q6ZQ38 |
| Gene Summary | Key assembly factor of SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complexes that promotes the exchange of the substrate-recognition F-box subunit in SCF complexes, thereby playing a key role in the cellular repertoire of SCF complexes. Acts as a F-box protein exchange factor. The exchange activity of CAND1 is coupled with cycles of neddylation conjugation: in the deneddylated state, cullin-binding CAND1 binds CUL1-RBX1, increasing dissociation of the SCF complex and promoting exchange of the F-box protein. Probably plays a similar role in other cullin-RING E3 ubiquitin ligase complexes (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224016 | Cand1 (untagged) - Mouse cullin associated and neddylation disassociated 1 (Cand1), (10ug) |
CNY 15,770.00 |
|
| MR216867 | Cand1 (Myc-DDK-tagged) - Mouse cullin associated and neddylation disassociated 1 (Cand1) |
CNY 12,260.00 |
|
| MR216867L3 | Lenti ORF clone of Cand1 (Myc-DDK-tagged) - Mouse cullin associated and neddylation disassociated 1 (Cand1) |
CNY 14,160.00 |
|
| MR216867L4 | Lenti ORF clone of Cand1 (mGFP-tagged) - Mouse cullin associated and neddylation disassociated 1 (Cand1) |
CNY 14,160.00 |
