Rab43 (NM_001039394) Mouse Tagged ORF Clone
CAT#: MG216803
- TrueORF®
Rab43 (tGFP-tagged) - Mouse RAB43 member RAS oncogene family (Rab43) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_001039394" in other vectors (4)
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CNY 2,850.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 1810048P08Rik; 2500004H21Rik; AW490415 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG216803 representing NM_001039394
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGGCCCTGGCCCGGGAGATCAGGACGAACACTACGATTTCCTGTTCAAGCTGGTGTTAGTGGGCG ACGCGAGCGTGGGCAAGACGTGCGTGGTGCAGCGCTTCAAGACCGGCGCCTTCTCGGCGCGTCAGGGCAG CACCATCGGTGTCGACTTCACCATGAAGACGCTGGAGATCCAGGGCAAGCGGGTCAAGTTACAGATTTGG GACACAGCTGGCCAGGAGCGGTTCCGGACCATCACCCAGAGCTACTACCGAAGTGCCAATGGGGCTATCC TTGCGTACGACATCAGCAAGAGGAGCACCTTCTTGTCAGTGCCTCACTGGATCGAGGATGTGAGGAAGTA CGCGGGTTCCAACATCGTGCAGCTGCTGATTGGGAACAAGTCAGACCTTGCCGATTTCCGGGAGGTCCCA TTGGCGGAGGCACAGAGCCTGGCTGAGCACTATGACATCCTCTGTGCCATCGAGACCTCCGCAAAGGACT CCAGCAATGTGGAGGAGGCCTTCACCAGAGTGGCCACGGAGCTCATCATGAGGCACGGAGGTCCCATGTT CAGTGAGAAGAACACAGACCACATCCAGCTGGACAGCAAGGACATTGCGGAGAGCTGGGGCTGTGGGTGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG216803 representing NM_001039394
Red=Cloning site Green=Tags(s) MAGPGPGDQDEHYDFLFKLVLVGDASVGKTCVVQRFKTGAFSARQGSTIGVDFTMKTLEIQGKRVKLQIW DTAGQERFRTITQSYYRSANGAILAYDISKRSTFLSVPHWIEDVRKYAGSNIVQLLIGNKSDLADFREVP LAEAQSLAEHYDILCAIETSAKDSSNVEEAFTRVATELIMRHGGPMFSEKNTDHIQLDSKDIAESWGCGC TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001039394 |
ORF Size | 630 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001039394.1, NP_001034483.1 |
RefSeq Size | 4605 bp |
RefSeq ORF | 633 bp |
Locus ID | 69834 |
UniProt ID | Q8CG50 |
Gene Summary | The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. The low intrinsic GTPase activity of RAB43 is activated by USP6NL. Involved in retrograde transport from the endocytic pathway to the Golgi apparatus. Involved in the transport of Shiga toxin from early and recycling endosomes to the trans-Golgi network. Required for the structural integrity of the Golgi complex. Plays a role in the maturation of phagosomes that engulf pathogens, such as S.aureus and Mycobacterium.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC211013 | Rab43 (untagged) - Mouse RAB43, member RAS oncogene family (Rab43), transcript variant 1, (10ug) |
CNY 3,990.00 |
|
MR216803 | Rab43 (Myc-DDK-tagged) - Mouse RAB43, member RAS oncogene family (Rab43), transcript variant 1 |
CNY 2,400.00 |
|
MR216803L3 | Lenti ORF clone of Rab43 (Myc-DDK-tagged) - Mouse RAB43, member RAS oncogene family (Rab43), transcript variant 1 |
CNY 4,750.00 |
|
MR216803L4 | Lenti ORF clone of Rab43 (mGFP-tagged) - Mouse RAB43, member RAS oncogene family (Rab43), transcript variant 1 |
CNY 4,750.00 |