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Ipo5 (NM_023579) Mouse Tagged ORF Clone
CAT＃: MG211627
- TrueORF®
Ipo5 (tGFP-tagged) - Mouse RAN binding protein 5 (Ranbp5)
ORF Plasmid: DDK
"NM_023579" in other vectors (4)
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CNY 10,550.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 1110011C18Rik; 5730478E03Rik; AA409333; C76941; IMB3; Kpnb3; Ranbp5 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG211627 representing NM_023579
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGGCCGCGGCGGAGCAGCAACAGTTCTACCTGCTCCTGGGAAACCTGCTCAGCCCCGACAATG TGGTCCGGAAACAGGCGGAGGAAACCTATGAGAATATCCCAGGCCGGTCCAAGATCACATTCCTCTTACA AGCCATCAGAAACACAACCGCTGCCGAGGAGGCTAGACAAATGGCTGCTGTTCTCCTAAGACGTCTTTTG TCCTCTGCATTTGATGAAGTCTACCCAGCTCTTCCATCAGATGTCCAGACTGCCATCAAGAGTGAATTGC TAATGATCATTCAGATGGAAACACAATCCAGCATGAGGAAGAAAATCTGTGATATTGCTGCAGAATTGGC CAGGAATTTAATAGATGAGGACGGCAACAACCAGTGGCCTGAAGGTTTGAAGTTCCTCTTTGATTCAGTC AGCTCACAAAACATGGGACTCCGGGAAGCTGCCCTTCACATATTTTGGAACTTTCCTGGAATTTTTGGGA ACCAACAACAGCACTATTTGGACGTCATCAAACGGATGTTAGTTCAGTGTATGCAAGATCAGGAGCATCC TTCGATCAGGACACTGTCTGCCAGAGCTACTGCTGCCTTCATACTTGCCAATGAGCATAATGTCGCTCTG TTCAAACACTTTGCAGACTTGCTGCCTGGATTCTTACAGGCTGTCAATGACTCATGCTACCAGAATGATG ACTCGGTCCTAAAATCCCTTGTTGAGATTGCAGACACCGTACCAAAGTACTTGCGCCCTCACTTAGAAGC AACTCTGCAGTTGAGTCTGAAGTTGTGTGGAGACACTAACCTCAATAATATGCAGCGCCAGCTTGCCCTT GAAGTGATTGTGACACTGTCAGAGACTGCAGCTGCTATGTTAAGAAAGCATACCAGTCTCATCGCACAGA CGATTCCTCAGATGTTGGCAATGATGGTTGACCTAGAAGAAGATGAGGACTGGGCAAATGCTGATGAGCT AGAAGATGATGATTTCGATAGCAATGCAGTTGCTGGTGAGAGTGCATTAGACCGAATGGCCTGTGGACTT GGTGGAAAGCTCGTTCTGCCCATGATTAAGGAACACATTATGCAAATGCTTCAAAACCCTGATTGGAAAT ACAGGCATGCAGGACTGATGGCCCTATCCGCCATTGGCGAAGGATGCCACCAGCAGATGGAAGGAATTCT AAATGAGATTGTAAATTTTGTCTTGCTTTTCCTCCAGGATCCTCATCCGAGAGTCCGGTATGCAGCCTGC AATGCTGTGGGTCAAATGGCTACAGATTTTGCACCTGGTTTTCAAAAGAAATTTCATGAGAAGGTGATTG CAGCTTTGCTTCAGACCATGGAAGACCAAGGCAACCAGCGGGTGCAGGCCCATGCAGCGGCTGCCCTCAT TAACTTCACTGAAGATTGTCCCAAGTCGCTGCTTATTCCCTACTTGGATAACTTGGTGAAGCACCTGCAC TCCATTATGGTGCTCAAGCTCCAGGAGCTGATTCAGAAAGGCACCAAGTTAGTTTTGGAACAAGTTGTGA CATCCATTGCATCAGTTGCAGATACTGCAGAAGAAAAGTTTGTCCCCTACTATGACTTGTTTATGCCATC ACTGAAGCACATTGTCGAGAACGCAGTCCAGAAGGAGCTGAGACTGCTGCGAGGGAAGACCATCGAGTGC ATCAGCCTCATCGGGCTGGCCGTCGGGAAGGAGAAATTCATGCAGGATGCTTCAGATGTGATGCAGCTAT TGTTGAAGACACAGACAGACTTCAATGATATGGAAGATGACGACCCCCAGATTTCTTACATGATCTCAGC ATGGGCCAGGATGTGCAAAATCCTTGGGAAAGAATTCCAGCAGTACCTTCCCGTGGTTATGGGGCCGCTG ATGAAGACTGCTTCAATTAAGCCTGAAGTGGCCCTTCTAGACACCCAGGACATGGAGAATATGAGTGATG ATGACGGTTGGGAATTTGTGAACCTCGGAGATCAGCAAAGTTTTGGTATTAAAACTGCAGGACTGGAAGA AAAGTCAACGGCTTGTCAGATGTTGGTTTGCTATGCTAAGGAATTAAAGGAAGGTTTTGTGGAATACACT GAACAGGTTGTCAAACTGATGGTCCCGCTGCTGAAATTTTATTTCCACGATGGGGTCCGAGTGGCAGCAG CAGAGTCCATGCCTCTTCTGCTTGAGTGTGCGAGAGTCCGGGGTCCCGAGTACCTTACACAGATGTGGCA CTTCATGTGCGACGCTCTCATCAAGGCCATCGGCACAGAGCCCGACTCAGATGTCCTCTCGGAGATCATG CATTCCTTTGCAAAGTGCATTGAGGTCATGGGAGACGGGTGCCTCAACAATGAGCACTTTGAGGAGCTGG GAGGCATCCTGAAGGCGAAGCTTGAAGAACATTTCAAAAATCAGGAGTTGCGGCAAGTTAAAAGACAAGA TGAAGACTATGACGAGCAGGTTGAAGAGTCACTACAAGATGAGGATGATAATGATGTTTATATACTGACT AAAGTGTCGGATATTTTACACTCAATATTCAGTAGCTACAAAGAAAAGGTGTTGCCGTGGTTTGAACAGC TGCTCCCATTAATTGTCAACCTGATTTGTCCGCAAAGACCATGGCCAGACAGACAATGGGGATTGTGCAT CTTCGATGATATCGTAGAACACTGTAGTCCAGCTTCATTTAAGTATGCAGAATATTTCATAAGTCCAATG CTCCAGTATGTGTGTGACAACAGCCCAGAAGTCAGGCAAGCTGCGGCATATGGCCTCGGCGTCATGGCGC AGTTTGGTGGAGATAACTACCGCCCTTTCTGTACCGATGCACTCCCGCTGCTTGTAAGAGTCATTCAGGC TCCAGAGGCTAAGACCAAAGAAAATGTCAACGCCACGGAGAACTGCATCTCAGCAGTGGGAAAGATCATG AAGTTCAAGCCCGACTGTGTGAATGTGGAGGAGGTCTTACCACACTGGCTGTCATGGCTGCCCCTACACG AAGATAAAGAAGAAGCTGTCCAGACCTTCAGTTACCTGTGCGACCTGATTGAAAGTAATCACCCAATTGT TCTTGGCCCAAACAATACCAATCTACCGAAAATATTCAGTATAATTGCAGAAGGAGAAATGCATGAGGCC ATTAAACATGAAGACCCCTGTGCCAAACGCCTGGCCAATGTAGTTCGCCAAGTACAGACTTCTGGAGGAT TGTGGACTGAATGCATAGCTCAACTCAGTCCGGAGCAGCAGGCAGCCATCCAGGAGCTCCTGAACTCTGC C ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG211627 representing NM_023579
Red=Cloning site Green=Tags(s) MAAAAAEQQQFYLLLGNLLSPDNVVRKQAEETYENIPGRSKITFLLQAIRNTTAAEEARQMAAVLLRRLL SSAFDEVYPALPSDVQTAIKSELLMIIQMETQSSMRKKICDIAAELARNLIDEDGNNQWPEGLKFLFDSV SSQNMGLREAALHIFWNFPGIFGNQQQHYLDVIKRMLVQCMQDQEHPSIRTLSARATAAFILANEHNVAL FKHFADLLPGFLQAVNDSCYQNDDSVLKSLVEIADTVPKYLRPHLEATLQLSLKLCGDTNLNNMQRQLAL EVIVTLSETAAAMLRKHTSLIAQTIPQMLAMMVDLEEDEDWANADELEDDDFDSNAVAGESALDRMACGL GGKLVLPMIKEHIMQMLQNPDWKYRHAGLMALSAIGEGCHQQMEGILNEIVNFVLLFLQDPHPRVRYAAC NAVGQMATDFAPGFQKKFHEKVIAALLQTMEDQGNQRVQAHAAAALINFTEDCPKSLLIPYLDNLVKHLH SIMVLKLQELIQKGTKLVLEQVVTSIASVADTAEEKFVPYYDLFMPSLKHIVENAVQKELRLLRGKTIEC ISLIGLAVGKEKFMQDASDVMQLLLKTQTDFNDMEDDDPQISYMISAWARMCKILGKEFQQYLPVVMGPL MKTASIKPEVALLDTQDMENMSDDDGWEFVNLGDQQSFGIKTAGLEEKSTACQMLVCYAKELKEGFVEYT EQVVKLMVPLLKFYFHDGVRVAAAESMPLLLECARVRGPEYLTQMWHFMCDALIKAIGTEPDSDVLSEIM HSFAKCIEVMGDGCLNNEHFEELGGILKAKLEEHFKNQELRQVKRQDEDYDEQVEESLQDEDDNDVYILT KVSDILHSIFSSYKEKVLPWFEQLLPLIVNLICPQRPWPDRQWGLCIFDDIVEHCSPASFKYAEYFISPM LQYVCDNSPEVRQAAAYGLGVMAQFGGDNYRPFCTDALPLLVRVIQAPEAKTKENVNATENCISAVGKIM KFKPDCVNVEEVLPHWLSWLPLHEDKEEAVQTFSYLCDLIESNHPIVLGPNNTNLPKIFSIIAEGEMHEA IKHEDPCAKRLANVVRQVQTSGGLWTECIAQLSPEQQAAIQELLNSA TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_023579 |
| ORF Size | 3291 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_023579.4, NP_076068.1 |
| RefSeq Size | 4598 bp |
| RefSeq ORF | 3294 bp |
| Locus ID | 70572 |
| UniProt ID | Q8BKC5 |
| Gene Summary | Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. Binds to CPEB3 and mediates its nuclear import following neuronal stimulation (PubMed:22730302).[UniProtKB/Swiss-Prot Function] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223537 | Ipo5 (untagged) - Mouse importin 5 (Ipo5), (10ug) |
CNY 14,060.00 |
|
| MR211627 | Ipo5 (Myc-DDK-tagged) - Mouse importin 5 (Ipo5) |
CNY 7,640.00 |
|
| MR211627L3 | Lenti ORF clone of Ipo5 (Myc-DDK-tagged) - Mouse importin 5 (Ipo5) |
CNY 11,500.00 |
|
| MR211627L4 | Lenti ORF clone of Ipo5 (mGFP-tagged) - Mouse importin 5 (Ipo5) |
CNY 11,500.00 |
