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Mcm8 (NM_025676) Mouse Tagged ORF Clone
CAT＃: MG210756
- TrueORF®
Mcm8 (tGFP-tagged) - Mouse minichromosome maintenance deficient 8 (S. cerevisiae) (Mcm8)
ORF Plasmid: DDK
"NM_025676" in other vectors (4)
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CNY 7,890.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 5730432L01Rik |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG210756 representing NM_025676
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGGTGCTTATAGAGGCAGAGGTTTTGGACGAGGAAGATTCCAAAGCTGGAAAAGAGGAAGAGGTG GTGGGAACTTCTCAGGAAGGTGGAGAGAAAGAGAAAACAGAGTTGACCTGAATGAAGCTTCAGGAAAGCA CGCTTCTGTTTACAGTAATAACTCTCCCTTTATTGAGAAGATTCAAGCATTTGAGAAATTTTTCACAAGG CATATCGACTTATACGATAAGGATGAAATAGAAAGAAAAGGAAGCATTTTGGTGGATTTTAAAGAACTGA CAAAAGCTGATGAAATAACTAACTTGATACCCGATATAGAAAACGCACTAAGAGATGCCCCTGAGAAAAC ACTGGCGTGTATGGGGCTGGCAATACATCAGGTATTAACTAAGGACCTTGAAAGGCATGCCGCCGAATTA CAAGCTCAAGAGGGATTGTCTAACGGTGGAGAGACAATGGTAAATGTACCACATATTTATGCAAGAGTGT ACAACTATGAGCCCTTGACACACCTCAAGAATATCCGAGCAACTTGCTATGGGAAATACATCTCTATACG AGGGACTGTGGTCCGGGTCAGCAACATAAAGCCTCTTTGTACCAACATGGCTTTTCAGTGCGCTGCATGT GGAGAGATTCAGAGCTTTCCTCTGCCAGATGGAAAATACACCCTTCCTACAAAGTGTCCTGTGCCTGCGT GCCGAGGGAGGTCATTTGCTCCACTGCGCAGCTCTCCTCTCACAGTTACACTGGACTGGCAGTTGATCAA AATCCAGGAGCTGATGTCTGATGCACAGAGAGAAGCTGGTCGGATCCCTCGGACGATAGAATGTGAACTT GTTCACGATCTTGTAGATAGTTGTGTCCCAGGAGATACAGTGACTGTTACTGGAATTGTCAAAGTCTCCA ATTCTGAAGAAGGTTCTCGAAATAAGAATGATAAGTGCATGTTCCTTTTGTACATTGAGGCAAATTCTGT TAGCAACAGCAAGGGGCCGAAAGCACAGACTGCAGAAGATGGTTGCAAGCATGGGACACTGATGGAGTTC TCCCTTAAAGACCTCTATGCCATCCGAGAGATCCAGGCTGAAGAGAACCTGCTCAAGCTCGTTGTCAACT CACTCTGTCCTGTCATTTTTGGTCATGAACTCGTCAAAGCAGGTCTGACGTTAGCACTCTTTGGCGGTAG CCAGAAGTACGCAGATGACAAAAACAGAATTCCCATTCGAGGAGACCCACATGTCCTGATTGTTGGAGAT CCAGGCTTGGGGAAGAGTCAGATGCTACAGGCAGCATGCAACGTGGCGCCACGTGGTGTGTATGTTTGTG GAAATACCACCACCAGCTCTGGTCTCACTGTGACTCTTTCAAAGGACAGTTCCTCTGGAGATTTTGCTTT GGAAGCTGGTGCCCTTGTACTTGGTGACCAAGGCATCTGTGGAATAGATGAATTTGATAAAATGGGGAAC CAACATCAAGCCCTGTTAGAAGCCATGGAACAGCAGAGTATTAGCCTTGCGAAGGCTGGGGTAGTTTGCA GCCTCCCTGCAAGAACTTCCATTATTGCTGCTGCAAATCCAGTCGGAGGACACTACAATAAAGCCAGAAC GGTTTCTGAGAATTTAAAGATGGGGAGTGCCCTACTGTCCAGATTCGATTTGGTCTTTATCTTGTTAGAT ACGCCAAATGAACAGCATGACCACTTACTTTCTGAACATGTGATCGCGATAAGAGCTGGGAAGCAGAAAG CGGTTAGCAGTGCCACAGTCACTCGTGTGCTCAGTCAAGACTCAAATACTTCTGTACTTGAAGTGGTTTC TGAGAAACCATTATCAGAAAGACTAAAGGTGGCTCCTGGAGAACAGACAGATCCGATTCCACATCAGCTG TTGAGGAAGTACATTGGCTATGCACGGCAGTATGTCCACCCAAGGTTATCTACGGACGCTGCTCAGGCCC TTCAGGATTTCTACCTGGAGCTCCGCAAGCAGAGCCAGCGCGTGGGCAGCTCACCCATCACCACTCGGCA GCTGGAGTCTTTGATCCGTCTGACAGAGGCACGGGCAAGGTTAGAATTGAGAGAGGAAGCAACTAGAGAA GATGCTGAAGATATAATTGAAATTATGAAGCATAGCATGCTAGGAACTTACTCAGATGAATTCGGAAACC TGGACTTTGAGCGATCCCAGCATGGCTCTGGGATGAGCAACAGGTCAACAGCAAAAAGATTTATTTCTGC TCTCAACAGCATTGCTGAAAGAACTTATAACAACATATTTCAATATCATCAACTTCGTCAGATTGCTAAA GAACTAAACATTCAGGTTGCCGATTTTGAAAACTTCATTGGATCACTGAATGACCAAGGTTATCTCTTGA AGAAAGGCCCAAAGATTTACCAACTTCAAACTATG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG210756 representing NM_025676
Red=Cloning site Green=Tags(s) MSGAYRGRGFGRGRFQSWKRGRGGGNFSGRWRERENRVDLNEASGKHASVYSNNSPFIEKIQAFEKFFTR HIDLYDKDEIERKGSILVDFKELTKADEITNLIPDIENALRDAPEKTLACMGLAIHQVLTKDLERHAAEL QAQEGLSNGGETMVNVPHIYARVYNYEPLTHLKNIRATCYGKYISIRGTVVRVSNIKPLCTNMAFQCAAC GEIQSFPLPDGKYTLPTKCPVPACRGRSFAPLRSSPLTVTLDWQLIKIQELMSDAQREAGRIPRTIECEL VHDLVDSCVPGDTVTVTGIVKVSNSEEGSRNKNDKCMFLLYIEANSVSNSKGPKAQTAEDGCKHGTLMEF SLKDLYAIREIQAEENLLKLVVNSLCPVIFGHELVKAGLTLALFGGSQKYADDKNRIPIRGDPHVLIVGD PGLGKSQMLQAACNVAPRGVYVCGNTTTSSGLTVTLSKDSSSGDFALEAGALVLGDQGICGIDEFDKMGN QHQALLEAMEQQSISLAKAGVVCSLPARTSIIAAANPVGGHYNKARTVSENLKMGSALLSRFDLVFILLD TPNEQHDHLLSEHVIAIRAGKQKAVSSATVTRVLSQDSNTSVLEVVSEKPLSERLKVAPGEQTDPIPHQL LRKYIGYARQYVHPRLSTDAAQALQDFYLELRKQSQRVGSSPITTRQLESLIRLTEARARLELREEATRE DAEDIIEIMKHSMLGTYSDEFGNLDFERSQHGSGMSNRSTAKRFISALNSIAERTYNNIFQYHQLRQIAK ELNIQVADFENFIGSLNDQGYLLKKGPKIYQLQTM TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_025676 |
| ORF Size | 2415 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_025676.2 |
| RefSeq Size | 3203 bp |
| RefSeq ORF | 2418 bp |
| Locus ID | 66634 |
| UniProt ID | Q9CWV1 |
| Gene Summary | Component of the MCM8-MCM9 complex, a complex involved in the repair of double-stranded DNA breaks (DBSs) and DNA interstrand cross-links (ICLs) by homologous recombination (HR). Required for DNA resection by the MRE11-RAD50-NBN/NBS1 (MRN) complex by recruiting the MRN complex to the repair site and by promoting the complex nuclease activity. Probably by regulating the localization of the MNR complex, indirectly regulates the recruitment of downstream effector RAD51 to DNA damage sites including DBSs and ICLs. The MCM8-MCM9 complex is dispensable for DNA replication and S phase progression. However, may play a non-essential for DNA replication: may be involved in the activation of the prereplicative complex (pre-RC) during G(1) phase by recruiting CDC6 to the origin recognition complex (ORC) (By similarity). Probably by regulating HR, plays a key role during gametogenesis (PubMed:22771120). Stabilizes MCM9 protein (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221745 | Mcm8 (untagged) - Mouse minichromosome maintenance deficient 8 (S. cerevisiae) (Mcm8), (10ug) |
CNY 10,360.00 |
|
| MR210756 | Mcm8 (Myc-DDK-tagged) - Mouse minichromosome maintenance deficient 8 (S. cerevisiae) (Mcm8) |
CNY 5,904.00 |
|
| MR210756L3 | Lenti ORF clone of Mcm8 (Myc-DDK-tagged) - Mouse minichromosome maintenance deficient 8 (S. cerevisiae) (Mcm8) |
CNY 9,030.00 |
|
| MR210756L4 | Lenti ORF clone of Mcm8 (mGFP-tagged) - Mouse minichromosome maintenance deficient 8 (S. cerevisiae) (Mcm8) |
CNY 9,030.00 |
