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Mcm8 (NM_025676) Mouse Untagged Clone
CAT＃: MC221745
Mcm8 (untagged) - Mouse minichromosome maintenance deficient 8 (S. cerevisiae) (Mcm8), (10ug)
CNY 10,360.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 5730432L01Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC221745 representing NM_025676
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGGTGCTTATAGAGGCAGAGGTTTTGGACGAGGAAGATTCCAAAGCTGGAAAAGAGGAAGAGGTG GTGGGAACTTCTCAGGAAGGTGGAGAGAAAGAGAAAACAGAGTTGACCTGAATGAAGCTTCAGGAAAGCA CGCTTCTGTTTACAGTAATAACTCTCCCTTTATTGAGAAGATTCAAGCATTTGAGAAATTTTTCACAAGG CATATCGACTTATACGATAAGGATGAAATAGAAAGAAAAGGAAGCATTTTGGTGGATTTTAAAGAACTGA CAAAAGCTGATGAAATAACTAACTTGATACCCGATATAGAAAACGCACTAAGAGATGCCCCTGAGAAAAC ACTGGCGTGTATGGGGCTGGCAATACATCAGGTATTAACTAAGGACCTTGAAAGGCATGCCGCCGAATTA CAAGCTCAAGAGGGATTGTCTAACGGTGGAGAGACAATGGTAAATGTACCACATATTTATGCAAGAGTGT ACAACTATGAGCCCTTGACACACCTCAAGAATATCCGAGCAACTTGCTATGGGAAATACATCTCTATACG AGGGACTGTGGTCCGGGTCAGCAACATAAAGCCTCTTTGCACCAACATGGCTTTTCAGTGCGCTGCATGT GGAGAGATTCAGAGCTTTCCTCTGCCAGATGGAAAATACACCCTTCCTACAAAGTGTCCTGTGCCTGCGT GCCGAGGGAGGTCATTTGCTCCACTGCGCAGCTCTCCTCTCACAGTTACACTGGACTGGCAGTTGATCAA AATCCAGGAGCTGATGTCTGATGCACAGAGAGAAGCTGGTCGGATCCCTCGGACGATAGAATGTGAACTT GTTCACGATCTTGTAGATAGTTGTGTCCCAGGAGATACAGTGACTGTTACTGGAATTGTCAAAGTCTCCA ATTCTGAAGAAGGTTCTCGAAATAAGAATGATAAGTGCATGTTCCTTTTGTACATTGAGGCAAATTCTGT TAGCAACAGCAAGGGGCCGAAAGCACAGACTGCAGAAGATGGTTGCAAGCATGGGACACTGATGGAGTTC TCCCTTAAAGACCTCTATGCCATCCGAGAGATCCAGGCTGAAGAGAACCTGCTCAAGCTCGTTGTCAACT CACTCTGTCCTGTCATTTTTGGTCATGAACTCGTCAAAGCAGGTCTGACGTTAGCACTCTTTGGCGGTAG CCAGAAGTACGCAGATGACAAAAACAGAATTCCCATTCGAGGAGACCCACATGTCCTGATTGTTGGAGAT CCAGGCTTGGGGAAGAGTCAGATGCTACAGGCAGCATGCAACGTGGCGCCACGTGGTGTGTATGTTTGTG GAAATACCACCACCAGCTCTGGTCTCACTGTGACTCTTTCAAAGGACAGTTCCTCTGGAGATTTTGCTTT GGAAGCTGGTGCCCTTGTACTTGGTGACCAAGGCATCTGTGGAATAGATGAATTTGATAAAATGGGGAAC CAACATCAAGCCCTGTTAGAAGCCATGGAACAGCAGAGTATTAGCCTTGCGAAGGCTGGGGTAGTTTGCA GCCTCCCTGCAAGAACTTCCATTATTGCTGCTGCAAATCCAGTCGGAGGACACTACAATAAAGCCAGAAC GGTTTCTGAGAATTTAAAGATGGGGAGTGCCCTACTGTCCAGATTCGATTTGGTCTTTATCTTGTTAGAT ACGCCAAATGAACAGCATGACCACTTACTTTCTGAACATGTGATCGCGATAAGAGCTGGGAAGCAGAAAG CGGTTAGCAGTGCCACAGTCACTCGTGTGCTCAGTCAAGACTCAAATACTTCTGTACTTGAAGTGGTTTC TGAGAAACCATTATCAGAAAGACTAAAGGTGGCTCCTGGAGAACAGACAGATCCGATTCCACATCAGCTG TTGAGGAAGTACATTGGCTATGCACGGCAGTATGTCCACCCAAGGTTATCTACGGACGCTGCTCAGGCCC TTCAGGATTTCTACCTGGAGCTCCGCAAGCAGAGCCAGCGCGTGGGCAGCTCACCCATCACCACTCGGCA GCTGGAGTCTTTGATCCGTCTGACAGAGGCACGGGCAAGGTTAGAATTGAGAGAGGAAGCAACTAGAGAA GATGCTGAAGATATAATTGAAATTATGAAGCATAGCATGCTAGGAACTTACTCAGATGAATTCGGAAACC TGGACTTTGAGCGATCCCAGCATGGCTCTGGGATGAGCAACAGGTCAACAGCAAAAAGATTTATTTCTGC TCTCAACAGCATTGCTGAAAGAACTTATAACAACATATTTCAATATCATCAACTTCGTCAGATTGCTAAA GAACTAAACATTCAGGTTGCCGATTTTGAAAACTTCATTGGATCACTGAATGACCAAGGTTATCTCTTGA AGAAAGGCCCAAAGATTTACCAACTTCAAACTATGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_025676 |
| Insert Size | 2418 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_025676.4, NP_079952.2 |
| RefSeq Size | 3397 bp |
| RefSeq ORF | 2418 bp |
| Locus ID | 66634 |
| UniProt ID | Q9CWV1 |
| Gene Summary | Component of the MCM8-MCM9 complex, a complex involved in the repair of double-stranded DNA breaks (DBSs) and DNA interstrand cross-links (ICLs) by homologous recombination (HR). Required for DNA resection by the MRE11-RAD50-NBN/NBS1 (MRN) complex by recruiting the MRN complex to the repair site and by promoting the complex nuclease activity. Probably by regulating the localization of the MNR complex, indirectly regulates the recruitment of downstream effector RAD51 to DNA damage sites including DBSs and ICLs. The MCM8-MCM9 complex is dispensable for DNA replication and S phase progression. However, may play a non-essential for DNA replication: may be involved in the activation of the prereplicative complex (pre-RC) during G(1) phase by recruiting CDC6 to the origin recognition complex (ORC) (By similarity). Probably by regulating HR, plays a key role during gametogenesis (PubMed:22771120). Stabilizes MCM9 protein (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1, resulting in an isoform (b) that is shorter than isoform a. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG210756 | Mcm8 (tGFP-tagged) - Mouse minichromosome maintenance deficient 8 (S. cerevisiae) (Mcm8) |
CNY 7,890.00 |
|
| MR210756 | Mcm8 (Myc-DDK-tagged) - Mouse minichromosome maintenance deficient 8 (S. cerevisiae) (Mcm8) |
CNY 5,904.00 |
|
| MR210756L3 | Lenti ORF clone of Mcm8 (Myc-DDK-tagged) - Mouse minichromosome maintenance deficient 8 (S. cerevisiae) (Mcm8) |
CNY 9,030.00 |
|
| MR210756L4 | Lenti ORF clone of Mcm8 (mGFP-tagged) - Mouse minichromosome maintenance deficient 8 (S. cerevisiae) (Mcm8) |
CNY 9,030.00 |
