United States
Germany
Japan
United Kingdom
China
Cop1 (NM_011931) Mouse Tagged ORF Clone
CAT＃: MG210351
- TrueORF®
Rfwd2 (tGFP-tagged) - Mouse ring finger and WD repeat domain 2 (Rfwd2)
ORF Plasmid: DDK
"NM_011931" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 6,370.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AI316802; C80879; Cop1; Rfwd2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG210351 representing NM_011931
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCAGGTAGCCGCCAGGCCGGCTCGGGTTCCGCTGGGACGAGCCCCGGCTCCTCGGCCGCCTCCTCGG TGACTTCCGCCTCCTCGTCCTTATCCTCGTCTCCGTCGCCGCCGTCCGTGGCGGCCTCGGCGGCGACGCT GGTGTCCGGCGGCGTGGCTCCGGCCGCGGGCTCCGGCGGCCTCGGCGGCCCGGGGCGGCCTGTGCTGGTG GCGGCCGCGGTGTCTGGCAGCGCGAGCGCCGGCGGGGCGGTGTCCGCGGGCCAGTCCCGGCTCAGCTGCG CGGCCAGGCCCAGCGCCGGCGTTGGAGGGAGCAGCTCCAGCCTCGGCAGTAGCAGTAGGAAGCGACCTCT GCTCGTCCCGCTTTGTAACGGGCTCCTCAACTCCTACGAGGACAAAAGCAACGACTTCGTCTGTCCCATC TGCTTTGATATGATTGAGGAGGCATACATGACAAAATGTGGCCACAGCTTTTGCTACAAGTGTATTCATC AGAGTTTGGAGGACAATAATAGATGTCCCAAGTGTAATTATGTTGTGGACAATATTGACCATCTCTATCC TAATTTCTTGGTGAATGAACTCATTCTCAAACAGAAGCAAAGATTTGAGGAAAAGAGGTTCAAATTGGAC CACTCAGTGAGTAGCACCAATGGGCATAGGTGGCAAATATTTCAAGATCTACTAGGAACTGATCAAGATA ACCTTGATTTGGCCAATGTCAACCTCATGTTGGAATTATTAGTGCAGAAGAAGAAACAACTGGAAGCAGA ATCACATGCAGCTCAGCTACAGATCCTTATGGAATTCCTCAAGGTTGCAAGGAGAAATAAGAGAGAGCAA TTGGAGCAGATCCAGAAGGAACTAAGTGTTTTGGAAGAGGATATTAAAAGAGTGGAAGAAATGAGTGGCC TCTACTCTCCTGTCAGTGAAGATAGCACAGTGCCTCAATTTGAAGCTCCTTCTCCATCACACAGTAGCAT TATTGATTCTACAGAATACAGCCAACCTCCAGGTTTCAGTGGGACCTCTCAGACAAAGAAACAGCCTTGG TATAACAGCACATTAGCATCAAGACGAAAGCGACTCACTGCTCATTTTGAAGACTTAGAGCAGTGTTATT TTTCTACAAGGATGTCTCGTATCTCAGATGACAGTAGAACTGCAAGCCAGTTAGATGAATTTCAAGAATG CTTGTCCAAGTTTACTCGATACAACTCAGTAAGACCGTTGGCCACATTGTCTTATGCTAGTGATCTCTAT AATGGTTCCAGCATAGTTTCTAGTATTGAGTTTGACCGGGATTGTGACTATTTTGCAATTGCTGGAGTTA CAAAGAAGATTAAAGTCTATGAGTATGGCACAGTCATCCAGGATGCAGTGGATATTCATTACCCTGAGAA TGAAATGACCTGCAATTCCAAAATCAGCTGTATCAGTTGGAGTAGTTACCATAAGAACCTGCTAGCCAGC AGTGATTATGAAGGCACTGTTATACTATGGGATGGATTCACAGGACAGAGGTCAAAGGTCTATCAGGAGC ATGAAAAAAGGTGTTGGAGTGTTGACTTTAATCTGATGGATCCTAAACTTCTGGCTTCAGGTTCTGATGA TGCAAAAGTGAAGCTGTGGTCTACCAATTTAGACAATTCTGTGGCGAGCATTGAGGCAAAGGCTAACGTG TGCTGTGTGAAGTTCAGCCCCTCCTCCAGGTACCATCTGGCTTTCGGCTGTGCAGATCACTGTGTCCACT ACTACGACCTTCGTAACACTAAACAGCCAATAATGGTATTCAAGGGACACCGAAAAGCAGTGTCTTATGC CAAGTTTGTAAGTGGTGAAGAAATTGTCTCAGCCTCAACAGACAGCCAACTAAAGCTGTGGAATGTGGGA AAACCATACTGTCTACGTTCCTTCAAGGGTCATATTAATGAAAAAAACTTTGTAGGCCTTGCTTCCAATG GAGATTATATAGCGTGTGGAAGTGAGAACAACTCCCTCTACCTGTATTATAAAGGACTTTCTAAAACTTT GCTAACTTTTAAGTTTGATACCGTTAAGAGTGTATTAGACAAAGATCGGAAAGAAGATGACACAAATGAA TTTGTCAGTGCTGTGTGTTGGCGGGCACTATCAGACGGGGAGTCCAATGTGCTGATTGCTGCTAACAGCC AGGGTACAATTAAGGTGCTAGAATTGGTA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG210351 representing NM_011931
Red=Cloning site Green=Tags(s) MSGSRQAGSGSAGTSPGSSAASSVTSASSSLSSSPSPPSVAASAATLVSGGVAPAAGSGGLGGPGRPVLV AAAVSGSASAGGAVSAGQSRLSCAARPSAGVGGSSSSLGSSSRKRPLLVPLCNGLLNSYEDKSNDFVCPI CFDMIEEAYMTKCGHSFCYKCIHQSLEDNNRCPKCNYVVDNIDHLYPNFLVNELILKQKQRFEEKRFKLD HSVSSTNGHRWQIFQDLLGTDQDNLDLANVNLMLELLVQKKKQLEAESHAAQLQILMEFLKVARRNKREQ LEQIQKELSVLEEDIKRVEEMSGLYSPVSEDSTVPQFEAPSPSHSSIIDSTEYSQPPGFSGTSQTKKQPW YNSTLASRRKRLTAHFEDLEQCYFSTRMSRISDDSRTASQLDEFQECLSKFTRYNSVRPLATLSYASDLY NGSSIVSSIEFDRDCDYFAIAGVTKKIKVYEYGTVIQDAVDIHYPENEMTCNSKISCISWSSYHKNLLAS SDYEGTVILWDGFTGQRSKVYQEHEKRCWSVDFNLMDPKLLASGSDDAKVKLWSTNLDNSVASIEAKANV CCVKFSPSSRYHLAFGCADHCVHYYDLRNTKQPIMVFKGHRKAVSYAKFVSGEEIVSASTDSQLKLWNVG KPYCLRSFKGHINEKNFVGLASNGDYIACGSENNSLYLYYKGLSKTLLTFKFDTVKSVLDKDRKEDDTNE FVSAVCWRALSDGESNVLIAANSQGTIKVLELV TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_011931 |
| ORF Size | 2199 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_011931.4 |
| RefSeq Size | 5056 bp |
| RefSeq ORF | 2202 bp |
| Locus ID | 26374 |
| UniProt ID | Q9R1A8 |
| Gene Summary | E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in JUN ubiquitination and degradation. Directly involved in p53 (TP53) ubiquitination and degradation, thereby abolishing p53-dependent transcription and apoptosis. Ubiquitinates p53 independently of MDM2 or RCHY1. Probably mediates E3 ubiquitin ligase activity by functioning as the essential RING domain subunit of larger E3 complexes. In contrast, it does not constitute the catalytic RING subunit in the DCX DET1-COP1 complex that negatively regulates JUN, the ubiquitin ligase activity being mediated by RBX1. Involved in 14-3-3 protein sigma/SFN ubiquitination and proteasomal degradation, leading to AKT activation and promotion of cell survival. Ubiquitinates MTA1 leading to its proteasomal degradation. Upon binding to TRIB1, ubiquitinates CEBPA, which lacks a canonical COP1-binding motif.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221164 | Rfwd2 (untagged) - Mouse ring finger and WD repeat domain 2 (Rfwd2), (10ug) |
CNY 9,500.00 |
|
| MR210351 | Rfwd2 (Myc-DDK-tagged) - Mouse ring finger and WD repeat domain 2 (Rfwd2) |
CNY 5,368.00 |
|
| MR210351L3 | Lenti ORF clone of Rfwd2 (Myc-DDK-tagged) - Mouse ring finger and WD repeat domain 2 (Rfwd2) |
CNY 7,700.00 |
|
| MR210351L4 | Lenti ORF clone of Rfwd2 (mGFP-tagged) - Mouse ring finger and WD repeat domain 2 (Rfwd2) |
CNY 7,700.00 |
