Stt3a (BC037612) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	AA408947; BB081708; Itm1
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG210156 representing BC037612 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTAAGCTTGGATTTTTGCGATTGTCCTATGAGAAGCAGGACACACTTCTAAAGCTTCTCATCCTGT CGATGGCTGCTGTGTTATCTTTTTCTACTCGTCTTTTTGCTGTGCTGAGATTTGAAAGTGTCATCCATGA GTTTGATCCGTACTTTAATTATCGGACTACCCGGTTTCTGGCTGAGGAGGGGTTTTATAAATTCCATAAC TGGTTTGATGACCGGGCTTGGTACCCTTTGGGCCGAATCATTGGAGGAACAATTTACCCAGGTTTAATGA TCACTTCTGCTGCAATCTACCATGTACTCCATTTCTTCCATATCACTATTGACATTCGGAATGTCTGTGT TTTCCTGGCCCCACTTTTCTCCTCTTTCACCACCATCGTTACGTACCACCTTACCAAAGAGCTCAAGGAT GCAGGAGCTGGGCTTCTTGCTGCTGCCATGATTGCTGTAGTTCCTGGGTATATTTCTCGATCTGTAGCTG GCTCCTATGATAATGAAGGAATTGCTATCTTTTGCATGCTGCTTACTTACTACATGTGGATCAAGGCAGT GAAGACTGGTTCCATCTATTGGGCTGCCAAGTGTGCCCTCGCTTATTTCTACATGGTCTCTTCATGGGGA GGCTATGTGTTCCTGATCAACTTGATTCCTCTACATGTCCTGGTGCTAATGCTGACAGGCCGTTTTTCTC ACCGGATCTACGTAGCCTACTGTACTGTTTACTGCCTGGGCACCATTCTTTCTATGCAGATTTCCTTTGT TGGTTTCCAGCCCGTCCTTTCATCAGAACACATGGCAGCCTTTGGAGTGTTTGGTCTCTGTCAGATCCAT GCTTTCGTAGATTACCTGCGCAGCAAGTTGAATCCACAGCAATTCGAAGTTCTTTTCCGGAGTGTTATCT CCCTGGTTGGCTTTGTCCTCCTCACTGTGGGAGCTCTCCTCATGCTAACAGGAAAAATTTCTCCCTGGAC AGGGCGTTTCTACTCTCTGCTGGATCCCTCTTATGCTAAGAATAACATTCCCATTATTGCATCTGTTTCT GAGCACCAGCCCACAACCTGGTCTTCCTACTATTTTGATCTACAGCTCCTTGTCTTCATGTTTCCAGTTG GCCTCTATTACTGCTTTAGCAACCTGTCTGATGCTCGGATTTTTATCATCATGTATGGTGTGACCAGCAT GTACTTTTCAGCTGTAATGGTGCGTCTAATGCTGGTATTGGCACCTGTTATGTGCATTCTTTCTGGCATT GGTGTTTCCCAGGTGCTGTCCACATATATGAAAAATCTGGACATAAGTCGCCCAGACAAGAAGAGCAAGA AGCAACAGGATTCTACTTACCCTATTAAGAATGAGGTGGCGAGTGGGATGATACTGGTCATGGCTTTTTT TCTCATCACCTACACGTTTCATTCGACTTGGGTGACCAGTGAAGCCTATTCTTCTCCCTCCATTGTACTG TCTGCTCGTGGTGGGGATGGCAGTAGGATCATTTTTGATGACTTCCGAGAAGCGTATTATTGGCTCCGTC ACAATACTCCAGAGGATGCAAAAGTCATGTCATGGTGGGATTATGGCTACCAAATTACTGCAATGGCAAA TCGGACAATTTTAGTGGACAATAACACATGGAATAATACCCATATTTCTCGAGTAGGGCAGGCAATGGCA TCCACAGAAGAAAAAGCCTATGAAATCATGAGGGAGCTTGATGTCAGCTATGTGCTTGTCATTTTTGGAG GCCTTACTGGGTATTCTTCGGATGATATCAACAAGTTTCTTTGGATGGTCCGGATTGGAGGAAGCACAGA GACAGGAAGACACATTAAGGAGAATGACTACTATACTCCTACTGGGGAATTCCGTGTTGATCGTGAGGGT TCTCCGGTGCTGCTCAACTGCCTTATGTACAAAATGTGTTACTACCGCTTTGGGCAGGTCTACACAGAAG CCAAGCGTCCACCAGGCTTTGACCGTGTTCGAAATGCTGAGATTGGTAATAAAGACTTTGAGCTTGATGT CCTGGAGGAAGCGTATACCACAGAACACTGGCTAGTCAGGATATACAAGGTAAAGGACCTGGATAATCGA GGCTTGTCAAGGACA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG210156 representing BC037612 Red=Cloning site Green=Tags(s) MTKLGFLRLSYEKQDTLLKLLILSMAAVLSFSTRLFAVLRFESVIHEFDPYFNYRTTRFLAEEGFYKFHN WFDDRAWYPLGRIIGGTIYPGLMITSAAIYHVLHFFHITIDIRNVCVFLAPLFSSFTTIVTYHLTKELKD AGAGLLAAAMIAVVPGYISRSVAGSYDNEGIAIFCMLLTYYMWIKAVKTGSIYWAAKCALAYFYMVSSWG GYVFLINLIPLHVLVLMLTGRFSHRIYVAYCTVYCLGTILSMQISFVGFQPVLSSEHMAAFGVFGLCQIH AFVDYLRSKLNPQQFEVLFRSVISLVGFVLLTVGALLMLTGKISPWTGRFYSLLDPSYAKNNIPIIASVS EHQPTTWSSYYFDLQLLVFMFPVGLYYCFSNLSDARIFIIMYGVTSMYFSAVMVRLMLVLAPVMCILSGI GVSQVLSTYMKNLDISRPDKKSKKQQDSTYPIKNEVASGMILVMAFFLITYTFHSTWVTSEAYSSPSIVL SARGGDGSRIIFDDFREAYYWLRHNTPEDAKVMSWWDYGYQITAMANRTILVDNNTWNNTHISRVGQAMA STEEKAYEIMRELDVSYVLVIFGGLTGYSSDDINKFLWMVRIGGSTETGRHIKENDYYTPTGEFRVDREG SPVLLNCLMYKMCYYRFGQVYTEAKRPPGFDRVRNAEIGNKDFELDVLEEAYTTEHWLVRIYKVKDLDNR GLSRT TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC037612
ORF Size	2117 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC037612, AAH37612
RefSeq Size	2736 bp
RefSeq ORF	2117 bp
Locus ID	16430
Gene Summary	Catalytic subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity. This subunit contains the active site and the acceptor peptide and donor lipid-linked oligosaccharide (LLO) binding pockets (By similarity). STT3A is present in the majority of OST complexes and mediates cotranslational N-glycosylation of most sites on target proteins, while STT3B-containing complexes are required for efficient post-translational glycosylation and mediate glycosylation of sites that have been skipped by STT3A (By similarity).[UniProtKB/Swiss-Prot Function]

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