Stt3a (BC037612) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AA408947; BB081708; Itm1
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC037612 CCACGCGTCCGGCGCCGCTGAACGGATGGCAGGGGGAGCAGAGTGGGTTCCTGAGGAGCATCCGTGAGGT ATTTGAATATCATCAGTTGCCACCCATTGATGTCAAGATGACTAAGCTTGGATTTTTGCGATTGTCCTAT GAGAAGCAGGACACACTTCTAAAGCTTCTCATCCTGTCGATGGCTGCTGTGTTATCTTTTTCTACTCGTC TTTTTGCTGTGCTGAGATTTGAAAGTGTCATCCATGAGTTTGATCCGTACTTTAATTATCGGACTACCCG GTTTCTGGCTGAGGAGGGGTTTTATAAATTCCATAACTGGTTTGATGACCGGGCTTGGTACCCTTTGGGC CGAATCATTGGAGGAACAATTTACCCAGGTTTAATGATCACTTCTGCTGCAATCTACCATGTACTCCATT TCTTCCATATCACTATTGACATTCGGAATGTCTGTGTTTTCCTGGCCCCACTTTTCTCCTCTTTCACCAC CATCGTTACGTACCACCTTACCAAAGAGCTCAAGGATGCAGGAGCTGGGCTTCTTGCTGCTGCCATGATT GCTGTAGTTCCTGGGTATATTTCTCGATCTGTAGCTGGCTCCTATGATAATGAAGGAATTGCTATCTTTT GCATGCTGCTTACTTACTACATGTGGATCAAGGCAGTGAAGACTGGTTCCATCTATTGGGCTGCCAAGTG TGCCCTCGCTTATTTCTACATGGTCTCTTCATGGGGAGGCTATGTGTTCCTGATCAACTTGATTCCTCTA CATGTCCTGGTGCTAATGCTGACAGGCCGTTTTTCTCACCGGATCTACGTAGCCTACTGTACTGTTTACT GCCTGGGCACCATTCTTTCTATGCAGATTTCCTTTGTTGGTTTCCAGCCCGTCCTTTCATCAGAACACAT GGCAGCCTTTGGAGTGTTTGGTCTCTGTCAGATCCATGCTTTCGTAGATTACCTGCGCAGCAAGTTGAAT CCACAGCAATTCGAAGTTCTTTTCCGGAGTGTTATCTCCCTGGTTGGCTTTGTCCTCCTCACTGTGGGAG CTCTCCTCATGCTAACAGGAAAAATTTCTCCCTGGACAGGGCGTTTCTACTCTCTGCTGGATCCCTCTTA TGCTAAGAATAACATTCCCATTATTGCATCTGTTTCTGAGCACCAGCCCACAACCTGGTCTTCCTACTAT TTTGATCTACAGCTCCTTGTCTTCATGTTTCCAGTTGGCCTCTATTACTGCTTTAGCAACCTGTCTGATG CTCGGATTTTTATCATCATGTATGGTGTGACCAGCATGTACTTTTCAGCTGTAATGGTGCGTCTAATGCT GGTATTGGCACCTGTTATGTGCATTCTTTCTGGCATTGGTGTTTCCCAGGTGCTGTCCACATATATGAAA AATCTGGACATAAGTCGCCCAGACAAGAAGAGCAAGAAGCAACAGGATTCTACTTACCCTATTAAGAATG AGGTGGCGAGTGGGATGATACTGGTCATGGCTTTTTTTCTCATCACCTACACGTTTCATTCGACTTGGGT GACCAGTGAAGCCTATTCTTCTCCCTCCATTGTACTGTCTGCTCGTGGTGGGGATGGCAGTAGGATCATT TTTGATGACTTCCGAGAAGCGTATTATTGGCTCCGTCACAATACTCCAGAGGATGCAAAAGTCATGTCAT GGTGGGATTATGGCTACCAAATTACTGCAATGGCAAATCGGACAATTTTAGTGGACAATAACACATGGAA TAATACCCATATTTCTCGAGTAGGGCAGGCAATGGCATCCACAGAAGAAAAAGCCTATGAAATCATGAGG GAGCTTGATGTCAGCTATGTGCTTGTCATTTTTGGAGGCCTTACTGGGTATTCTTCGGATGATATCAACA AGTTTCTTTGGATGGTCCGGATTGGAGGAAGCACAGAGACAGGAAGACACATTAAGGAGAATGACTACTA TACTCCTACTGGGGAATTCCGTGTTGATCGTGAGGGTTCTCCGGTGCTGCTCAACTGCCTTATGTACAAA ATGTGTTACTACCGCTTTGGGCAGGTCTACACAGAAGCCAAGCGTCCACCAGGCTTTGACCGTGTTCGAA ATGCTGAGATTGGTAATAAAGACTTTGAGCTTGATGTCCTGGAGGAAGCGTATACCACAGAACACTGGCT AGTCAGGATATACAAGGTAAAGGACCTGGATAATCGAGGCTTGTCAAGGACATAAACGTCACATTGTGCC CTGAGCATTATGCTTCGCACTGAGCGCGTCATGTTGAGGACGCTGAAGATGTTTTTTATATGCAGTTTAT AAGAACAGCCGGATGGGGTTAGAATTGTCTGCAAGTTTTGCCCTGGACAATATGGGCTGGGCCAAGTGAA ATGATTTTTATAATTCTGAGCAGGTTACCAAATGAAATGTTATGGCTTTACTTTGGTCAATTAAAAGAGG GGGGGGGATTTTTTTTAAATGTGCCTTATTTGTTTTGACTTAAATTGGCTGATACGAGGATCACAGAAGT GAGCGGATGGAAGACCATATCCATGCTCTAGGTCCCCAAATGAACCAGATAGGAGCATTTTTTTCTCCTA TCAGCAATCTCAAGGACTAGCTCTGGTTCAACAAATGTAAACAACAACTTTGTCACACTTTTTTGTTTTT TAGCACCCAGGTACAATGCTTTCCTTATAATGGGTGCTTAATAAATTTTTATCAAATGAATAAAAAAAAA AAAAAA
Restriction Sites	RsrII-NotI
ACCN	BC037612
Insert Size	2118 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC037612, AAH37612
RefSeq Size	2736 bp
RefSeq ORF	2118 bp
Locus ID	16430
Gene Summary	Catalytic subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity. This subunit contains the active site and the acceptor peptide and donor lipid-linked oligosaccharide (LLO) binding pockets (By similarity). STT3A is present in the majority of OST complexes and mediates cotranslational N-glycosylation of most sites on target proteins, while STT3B-containing complexes are required for efficient post-translational glycosylation and mediate glycosylation of sites that have been skipped by STT3A (By similarity).[UniProtKB/Swiss-Prot Function]

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