Casp8 (NM_009812) Mouse Tagged ORF Clone
CAT#: MG207700
- TrueORF®
Casp8 (tGFP-tagged) - Mouse caspase 8 (Casp8), transcript variant 1
"NM_009812" in other vectors (1)
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CNY 7,088.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | Casp; CASP-8; FLI; FLICE; MAC; MACH; Mch5 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG207700 representing NM_009812
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATTTCCAGAGTTGTCTTTATGCTATTGCTGAAGAACTGGGCAGTGAAGACCTGGCTGCCCTCAAGT TCCTGTGCTTGGACTACATCCCACACAAGAAGCAGGAGACCATCGAGGATGCCCAGAAGCTATTTCTGAG GCTGCGGGAAAAGGGGATGTTGGAGGAAGGCAATCTGTCTTTCCTGAAAGAGCTGCTTTTCCACATCAGT CGGTGGGACCTGCTGGTCAACTTCCTAGACTGCAACCGAGAGGAGATGGTGAGAGAGCTGCGGGATCCAG ACAATGCCCAGATTTCTCCCTACAGGGTCATGCTCTTTAAGCTCTCAGAAGAAGTGAGCGAGTTGGAATT GAGATCTTTTAAGTTCCTTTTGAACAATGAGATCCCCAAATGTAAGCTGGAAGATGACTTGAGCCTGCTT GAAATTTTTGTAGAAATGGAGAAGAGGACCATGCTGGCAGAAAATAACTTGGAAACCCTAAAATCAATCT GTGACCAGGTCAACAAGAGCCTGCTGGGGAAGATCGAGGATTATGAAAGATCAAGCACAGAGAGAAGAAT GAGCCTTGAAGGAAGGGAAGAGTTGCCACCTTCAGTTTTGGATGAGATGAGCCTCAAAATGGCGGAACTG TGTGACTCGCCAAGAGAACAAGACAGTGAGTCACGGACTTCAGACAAAGTTTACCAAATGAAGAACAAAC CTCGGGGATACTGTCTGATCATCAACAATCATGATTTCAGCAAGGCCCGGGAAGACATAACCCAACTCCG AAAAATGAAGGACAGAAAAGGAACAGACTGTGATAAAGAGGCTCTGAGTAAGACCTTTAAGGAGCTTCAT TTTGAGATAGTATCTTACGACGACTGCACTGCAAATGAAATCCACGAGATTCTAGAAGGCTACCAAAGCG CAGACCACAAGAACAAAGACTGCTTCATCTGCTGTATCCTATCCCACGGTGACAAGGGTGTCGTCTATGG AACGGATGGGAAGGAGGCCTCCATCTATGACCTGACATCTTACTTCACTGGTTCAAAGTGCCCTTCCCTG TCTGGGAAACCCAAGATCTTTTTCATTCAGGCTTGCCAAGGAAGTAACTTCCAGAAAGGAGTGCCTGATG AGGCAGGCTTCGAGCAACAGAACCACACTTTAGAAGTGGATTCATCATCTCACAAGAACTATATTCCGGA TGAGGCAGACTTTCTGCTGGGAATGGCTACGGTGAAGAACTGCGTTTCCTACCGAGATCCTGTGAATGGA ACCTGGTATATTCAGTCACTTTGCCAGAGCCTGAGGGAAAGATGTCCTCAAGGAGATGACATTCTTAGCA TCCTGACTGGCGTGAACTATGACGTGAGCAATAAAGACGACAGGAGGAACAAGGGAAAGCAGATGCCACA GCCCACCTTCACACTACGGAAGAAGCTCTTCTTCCCTCCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG207700 representing NM_009812
Red=Cloning site Green=Tags(s) MDFQSCLYAIAEELGSEDLAALKFLCLDYIPHKKQETIEDAQKLFLRLREKGMLEEGNLSFLKELLFHIS RWDLLVNFLDCNREEMVRELRDPDNAQISPYRVMLFKLSEEVSELELRSFKFLLNNEIPKCKLEDDLSLL EIFVEMEKRTMLAENNLETLKSICDQVNKSLLGKIEDYERSSTERRMSLEGREELPPSVLDEMSLKMAEL CDSPREQDSESRTSDKVYQMKNKPRGYCLIINNHDFSKAREDITQLRKMKDRKGTDCDKEALSKTFKELH FEIVSYDDCTANEIHEILEGYQSADHKNKDCFICCILSHGDKGVVYGTDGKEASIYDLTSYFTGSKCPSL SGKPKIFFIQACQGSNFQKGVPDEAGFEQQNHTLEVDSSSHKNYIPDEADFLLGMATVKNCVSYRDPVNG TWYIQSLCQSLRERCPQGDDILSILTGVNYDVSNKDDRRNKGKQMPQPTFTLRKKLFFPP TRTRPLE - GFP Tag - V |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_009812 |
ORF Size | 1440 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_009812.2 |
RefSeq Size | 2585 bp |
RefSeq ORF | 1443 bp |
Locus ID | 12370 |
UniProt ID | O89110 |
Gene Summary | This gene is part of a family of caspases, aspartate-specific cysteine proteases well studied for their involvement in immune and apoptosis signaling. This protein, an initiator of apoptotic cell death, is activated by death-inducing tumor necrosis family receptors and targets downstream effectors. In mouse deficiency of this gene can cause embryonic lethality. This protein may have a role in embryogenesis. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Apr 2013] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC200404 | Casp8 (untagged) - Mouse caspase 8 (Casp8), transcript variant 1, (10ug) |
CNY 5,488.00 |