Casp8 (NM_009812) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Casp; CASP-8; FLI; FLICE; MAC; MACH; Mch5
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC006737 sequence for NM_009812 GCAGCCTATGGCCTCTAGGGCTGGCACCAGGATGCCACCTCTAATGATTAACTCAAACGCCTCTAAACAT TCGGAGGCATTTCTGTCCCCTATGCCCTAGTTCTCTCAGTTGTCTTTCATTCTGACTTCGGTGCTTAAAA GTCCAGCTTCTCGGAATCGGTAGCAAACCTCTGTGAGCCGGCGTGGAACAGGAAGTGAGTACAGTTCTGG GGAGCGCCGGCCCGGGCTGGAGGCTCGGAAAGCCCAAGCCAGCGGAGGCCTCGCCCGAGCTGGAGTTGTG ACCGGCGCGGCAGGTACTCGGCCACAGGTTACAGCTCTTCTACCTCTTGATAAGAATGGATTTCCAGAGT TGTCTTTATGCTATTGCTGAAGAACTGGGCAGTGAAGACCTGGCTGCCCTCAAGTTCCTGTGCTTGGACT ACATCCCACACAAGAAGCAGGAGACCATCGAGGATGCCCAGAAGCTATTTCTGAGGCTGCGGGAAAAGGG GATGTTGGAGGAAGGCAATCTGTCTTTCCTGAAAGAGCTGCTTTTCCACATCAGTCGGTGGGACCTGCTG GTCAACTTCCTAGACTGCAACCGAGAGGAGATGGTGAGAGAGCTGCGGGATCCAGACAATGCCCAGATTT CTCCCTACAGGGTCATGCTCTTTAAGCTCTCAGAAGAAGTGAGCGAGTTGGAATTGAGATCTTTTAAGTT CCTTTTGAACAATGAGATCCCCAAATGTAAGCTGGAAGATGACTTGAGCCTGCTTGAAATTTTTGTAGAA ATGGAGAAGAGGACCATGCTGGCAGAAAATAACTTGGAAACCCTAAAATCAATCTGTGACCAGGTCAACA AGAGCCTGCTGGGGAAGATCGAGGATTATGAAAGATCAAGCACAGAGAGAAGAATGAGCCTTGAAGGAAG GGAAGAGTTGCCACCTTCAGTTTTGGATGAGATGAGCCTCAAAATGGCGGAACTGTGTGACTCGCCAAGA GAACAAGACAGTGAGTCACGGACTTCAGACAAAGTTTACCAAATGAAGAACAAACCTCGGGGATACTGTC TGATCATCAACAATCATGATTTCAGCAAGGCCCGGGAAGACATAACCCAACTCCGAAAAATGAAGGACAG AAAAGGAACAGACTGTGATAAAGAGGCTCTGAGTAAGACCTTTAAGGAGCTTCATTTTGAGATAGTATCT TACGACGACTGCACTGCAAATGAAATCCACGAGATTCTAGAAGGCTACCAAAGCGCAGACCACAAGAACA AAGACTGCTTCATCTGCTGTATCCTATCCCACGGTGACAAGGGTGTCGTCTATGGAACGGATGGGAAGGA GGCCTCCATCTATGACCTGACATCTTACTTCACTGGTTCAAAGTGCCCTTCCCTGTCTGGGAAACCCAAG ATCTTTTTCATTCAGGCTTGCCAAGGAAGTAACTTCCAGAAAGGAGTGCCTGATGAGGCAGGCTTCGAGC AACAGAACCACACTTTAGAAGTGGATTCATCATCTCACAAGAACTATATTCCGGATGAGGCAGACTTTCT GCTGGGAATGGCTACGGTGAAGAACTGCGTTTCCTACCGAGATCCTGTGAATGGAACCTGGTATATTCAG TCACTTTGCCAGAGCCTGAGGGAAAGATGTCCTCAAGGAGATGACATTCTTAGCATCCTGACTGGCGTGA ACTATGACGTGAGCAATAAAGACGACAGGAGGAACAAGGGAAAGCAGATGCCACAGCCCACCTTCACACT ACGGAAGAAGCTCTTCTTCCCTCCCTAATGATGTGTGCTCTCCACAGTTCACATGGCTTATCTGTGCACT TTTGTGTGGATGAGTCTAATTTATTTTTTAGAATTTCTTTTGCTTTTGAATTTACATTTACATAATTTTC CCTTTTCTTCCCTTTAAACCCTTCTTTGTTATGTTCCAATTTCAAATACATGGCCTCTTTTCTCATTAAC TGTTGTACACACACATACATACACACACACACACACACACACACATTTCTAAATATAACCTGTATACTAT CAAAAAAAAAAAAAAA
Restriction Sites	RsrII-NotI
ACCN	NM_009812
Insert Size	1443 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC006737, AAH06737
RefSeq Size	2046 bp
RefSeq ORF	1443 bp
Locus ID	12370
UniProt ID	O89110
Gene Summary	This gene is part of a family of caspases, aspartate-specific cysteine proteases well studied for their involvement in immune and apoptosis signaling. This protein, an initiator of apoptotic cell death, is activated by death-inducing tumor necrosis family receptors and targets downstream effectors. In mouse deficiency of this gene can cause embryonic lethality. This protein may have a role in embryogenesis. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Apr 2013] Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 both encode isoform (1).

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