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Ryk (BC032275) Mouse Tagged ORF Clone
CAT＃: MG207613
- TrueORF®
Ryk (tGFP-tagged) - Mouse receptor-like tyrosine kinase (cDNA clone MGC:40871 IMAGE:5370100)
ORF Plasmid: DDK
"BC032275" in other vectors (4)
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CNY 3,040.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Vik, ERK-3 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG207613 representing BC032275
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCATGCCCCAGGTCAATATTTCTGCTCAAGGGGAGGTCCCACGCACTTTATCAGTGTTTCGGGTCG AGCTTTCTTGTACCGGCAAAGTCGACTCTGAAGTCATGATTCTAATGCAGCTCAATCTGACAGTGAATTC CTCAAAAAATTTTACAGTTTTAAATTTTAAACGAAGGAAAATGTGCTACAAAAAACTTGAAGAAGTAAAA ACTTCAGCCTTGGACAAAAACACTAGCAGAACTATTTATGACCCTGTCCATGCAGCGCCAACGACTTCCA CGCGTGTGTTTTACATCAGTGTAGGGGTTTGCTGTGCAGTGATATTTCTTGTAGCAATAATATTAGCCGT TTTGCACCTTCATAGCATGAAAAGGATTGAACTGGATGACAGCATCAGCGCCAGCAGTAGTTCCCAGGGG CTGTCTCAGCCGTCTACCCAGACGACCCAGTATCTGAGAGCTGACACACCCAACAATGCAACGCCTATCA CCAGTTATCCTACCTTGCGGATAGAGAAGAACGACTTGCGAAGTGTCACTCTTCTGGAAGCCAAAGCCAA GGTGAAGGATATCGCAATATCCAGAGAAAGGATCACACTGAAAGATGTCCTCCAAGAAGGTACTTTTGGG CGTATTTTCCATGGGATTTTAGTAGATGAAAAAGATCCAAATAAAGAGAAGCAAACATTTGTAAAAACAG TTAAAGACCAAGCATCTGAAGTTCAGGTGACGATGATGCTCACCGAGAGCTGCAAGCTTCGAGGTCTGCA CCACAGAAACCTCCTTCCTATTACTCATGTGTGCATAGAAGAAGGAGAAAAGCCCATGGTGGTATTGCCA TACATGAATTGGGGGAATCTTAAATTATTTCTTCGGCAGTGCAAATTAGTAGAAGCCAATAATCCACAGG CAATTTCCCAGCAAGATCTGGTCCATATGGCTATTCAGATTGCCTGCGGGATGAGCTACCTGGCGAGGAG AGAAGTGATCCATAGAGACCTGGCTGCTAGGAACTGTGTCATCGACGACACTCTTCAAGTCAAGATCACA GACAATGCCCTTTCCAGAGACTTGTTTCCTATGGACTACCACTGCCTAGGGGACAACGAGAACAGGCCAG TGAGATGGATGGCTCTGGAAAGTCTGGTTAATAATGAGTTCTCTAGTGCTAGTGACGTGTGGGCCTTTGG AGTGACGCTGTGGGAGCTCATGACTCTGGGCCAGACGCCCTACGTGGACATCGACCCCTTTGAGATGGCC GCTTACCTGAAAGATGGTTACCGAATAGCCCAGCCAATCAACTGCCCTGATGAACTGTTTGCTGTGATGG CCTGTTGCTGGGCCTTGGACCCTGAGGAGAGGCCTAAGTTCCAGCAGCTGGTCCAGTGCCTCACAGAGTT CCACGCTGCCCTGGGAGCCTACGTC AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG207613 representing BC032275
Red=Cloning site Green=Tags(s) MGMPQVNISAQGEVPRTLSVFRVELSCTGKVDSEVMILMQLNLTVNSSKNFTVLNFKRRKMCYKKLEEVK TSALDKNTSRTIYDPVHAAPTTSTRVFYISVGVCCAVIFLVAIILAVLHLHSMKRIELDDSISASSSSQG LSQPSTQTTQYLRADTPNNATPITSYPTLRIEKNDLRSVTLLEAKAKVKDIAISRERITLKDVLQEGTFG RIFHGILVDEKDPNKEKQTFVKTVKDQASEVQVTMMLTESCKLRGLHHRNLLPITHVCIEEGEKPMVVLP YMNWGNLKLFLRQCKLVEANNPQAISQQDLVHMAIQIACGMSYLARREVIHRDLAARNCVIDDTLQVKIT DNALSRDLFPMDYHCLGDNENRPVRWMALESLVNNEFSSASDVWAFGVTLWELMTLGQTPYVDIDPFEMA AYLKDGYRIAQPINCPDELFAVMACCWALDPEERPKFQQLVQCLTEFHAALGAYV SGPTRTRRLE - GFP Tag - V |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC032275 |
| ORF Size | 1427 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC032275, AAH32275 |
| RefSeq Size | 2006 bp |
| RefSeq ORF | 1427 bp |
| Locus ID | 20187 |
| Gene Summary | May be a coreceptor along with FZD8 of Wnt proteins, such as WNT1, WNT3, WNT3A and WNT5A. Involved in neuron differentiation, axon guidance, corpus callosum establishment and neurite outgrowth. In response to WNT3 stimulation, receptor C-terminal cleavage occurs in its transmembrane region and allows the C-terminal intracellular product to translocate from the cytoplasm to the nucleus where it plays a crucial role in neuronal development.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218103 | Ryk (untagged) - Mouse receptor-like tyrosine kinase (cDNA clone MGC:40871 IMAGE:5370100), (10ug) |
CNY 6,940.00 |
|
| MR207613 | Ryk (Myc-DDK-tagged) - Mouse receptor-like tyrosine kinase (cDNA clone MGC:40871 IMAGE:5370100) |
CNY 3,656.00 |
|
| MR207613L3 | Lenti ORF clone of Ryk (Myc-DDK-tagged) - Mouse receptor-like tyrosine kinase (cDNA clone MGC:40871 IMAGE:5370100) |
CNY 6,056.00 |
|
| MR207613L4 | Lenti ORF clone of Ryk (mGFP-tagged) - Mouse receptor-like tyrosine kinase (cDNA clone MGC:40871 IMAGE:5370100) |
CNY 4,660.00 |
