Ryk (BC032275) Mouse Untagged Clone

CAT#: MC218103

Ryk (untagged) - Mouse receptor-like tyrosine kinase (cDNA clone MGC:40871 IMAGE:5370100), (10ug)



  "BC032275" in other vectors (4)

CNY 6,940.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms Vik, ERK-3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>BC032275
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCATGCCCCAGGTCAATATTTCTGCTCAAGGGGAGGTCCCACGCACTTTATCAGTGTTTCGGGTCG
AGCTTTCTTGTACCGGCAAAGTCGACTCTGAAGTCATGATTCTAATGCAGCTCAATCTGACAGTGAATTC
CTCAAAAAATTTTACAGTTTTAAATTTTAAACGAAGGAAAATGTGCTACAAAAAACTTGAAGAAGTAAAA
ACTTCAGCCTTGGACAAAAACACTAGCAGAACTATTTATGACCCTGTCCATGCAGCGCCAACGACTTCCA
CGCGTGTGTTTTACATCAGTGTAGGGGTTTGCTGTGCAGTGATATTTCTTGTAGCAATAATATTAGCCGT
TTTGCACCTTCATAGCATGAAAAGGATTGAACTGGATGACAGCATCAGCGCCAGCAGTAGTTCCCAGGGG
CTGTCTCAGCCGTCTACCCAGACGACCCAGTATCTGAGAGCTGACACACCCAACAATGCAACGCCTATCA
CCAGTTATCCTACCTTGCGGATAGAGAAGAACGACTTGCGAAGTGTCACTCTTCTGGAAGCCAAAGCCAA
GGTGAAGGATATCGCAATATCCAGAGAAAGGATCACACTGAAAGATGTCCTCCAAGAAGGTACTTTTGGG
CGTATTTTCCATGGGATTTTAGTAGATGAAAAAGATCCAAATAAAGAGAAGCAAACATTTGTAAAAACAG
TTAAAGACCAAGCATCTGAAGTTCAGGTGACGATGATGCTCACCGAGAGCTGCAAGCTTCGAGGTCTGCA
CCACAGAAACCTCCTTCCTATTACTCATGTGTGCATAGAAGAAGGAGAAAAGCCCATGGTGGTATTGCCA
TACATGAATTGGGGGAATCTTAAATTATTTCTTCGGCAGTGCAAATTAGTAGAAGCCAATAATCCACAGG
CAATTTCCCAGCAAGATCTGGTCCATATGGCTATTCAGATTGCCTGCGGGATGAGCTACCTGGCGAGGAG
AGAAGTGATCCATAGAGACCTGGCTGCTAGGAACTGTGTCATCGACGACACTCTTCAAGTCAAGATCACA
GACAATGCCCTTTCCAGAGACTTGTTTCCTATGGACTACCACTGCCTAGGGGACAACGAGAACAGGCCAG
TGAGATGGATGGCTCTGGAAAGTCTGGTTAATAATGAGTTCTCTAGTGCTAGTGACGTGTGGGCCTTTGG
AGTGACGCTGTGGGAGCTCATGACTCTGGGCCAGACGCCCTACGTGGACATCGACCCCTTTGAGATGGCC
GCTTACCTGAAAGATGGTTACCGAATAGCCCAGCCAATCAACTGCCCTGATGAACTGTTTGCTGTGATGG
CCTGTTGCTGGGCCTTGGACCCTGAGGAGAGGCCTAAGTTCCAGCAGCTGGTCCAGTGCCTCACAGAGTT
CCACGCTGCCCTGGGAGCCTACGTCTGA


AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATA
TCCTGGATTACAAGGATGACGACGATAAG
GTTTAA
Restriction Sites SgfI-RsrII     
ACCN BC032275
Insert Size 1428 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC032275, AAH32275
RefSeq Size 2006 bp
RefSeq ORF 1427 bp
Locus ID 20187
Gene Summary May be a coreceptor along with FZD8 of Wnt proteins, such as WNT1, WNT3, WNT3A and WNT5A. Involved in neuron differentiation, axon guidance, corpus callosum establishment and neurite outgrowth. In response to WNT3 stimulation, receptor C-terminal cleavage occurs in its transmembrane region and allows the C-terminal intracellular product to translocate from the cytoplasm to the nucleus where it plays a crucial role in neuronal development.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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