Sox10 (BC018551) Mouse Tagged ORF Clone

CAT#: MG207447

  • TrueORF®

Sox10 (tGFP-tagged) - Mouse SRY-box containing gene 10 (cDNA clone MGC:18351 IMAGE:3675437)

ORF Plasmid: DDK tGFP



  "BC018551" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 2,950.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


Sox10 Antibody - N-terminal region
    • 50 ug

CNY 4,628.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms Dom; gt; Sox21
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG207447 representing BC018551
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGAGGAACAAGACCTATCAGAGGTGGAGCTGAGCCCTGTGGGCTCGGAGGAACCCCGCTGCCTGT
CCCCAGGCAGCGCGCCGTCGCTGGGACCCGACGGCGGCGGCGGTGGCTCGGGCTTGCGAGCCAGCCCGGG
GCCCGGTGAACTGGGCAAGGTCAAGAAGGAACAGCAGGACGGCGAGGCGGACGATGACAAGTTCCCCGTG
TGCATCCGCGAGGCGGTCAGCCAGGTGCTCAGCGGCTACGACTGGACGCTGGTGCCCATGCCCGTGCGCG
TCAACGGTGCCAGCAAGAGCAAGCCGCACGTCAAGAGGCCCATGAACGCCTTCATGGTGTGGGCACAGGC
GGCACGCAGAAAGCTAGCCGACCAGTACCCTCACCTCCACAATGCTGAGCTCAGCAAGACACTAGGCAAG
CTCTGGAGGTTGCTGAACGAAAGTGACAAGCGCCCCTTCATTGAGGAGGCTGAGCGGCTCCGGATGCAGC
ACAAAAAGGACCATCCGGACTACAAGTACCAACCTCGGCGGCGGAAGAACGGGAAGGCAGCCCAGGGGGA
GGCAGAATGCCCAGGCGGGGAAGCCGAGCAAGGAGGGGCTGCTGCTATTCAGGCTCACTACAAGAGTGCC
CACCTGGACCACCGGCACCCAGAAGAAGGCTCCCCCATGTCAGATGGGAACCCAGAGCACCCCTCAGGCC
AGAGCCATGGCCCCCCAACCCCTCCAACCACCCCAAAGACAGAGCTGCAGTCCGGCAAGGCAGACCCCAA
AAGGGATGGGCGCTCCTTGGGGGAGGGCGGGAAGCCCCACATCGACTTCGGCAACGTGGACATCGGGGAG
ATCAGCCACGAGGTAATGTCCAACATGGAAACCTTTGATGTGACTGAGCTGGACCAATACCTGCCACCCA
ACGGGCACCCAGGCCATGTGGGTAGCTACTCGGCAGCTGGCTATGGGCTGGGCAGTGCCCTGGCTGTGGC
CAGTGGACACTCTGCCTGGATCTCCAAGCCACCAGGTGTGGCTCTGCCCACGGTCTCGCCCCCTGGTGTG
GATGCCAAAGCCCAGGTGAAGACAGAGACCACAGGCCCCCAGGGACCCCCACACTACACCGACCAGCCGT
CCACTTCCCAGATCGCCTACACCTCCCTCAGTCTGCCCCACTACGGCTCCGCCTTCCCCTCCATCTCACG
ACCCCAGTTTGACTATTCTGACCATCAGCCCTCAGGACCCTATTATGGCCATGCAGGCCAGGCCTCTGGC
CTCTATTCAGCCTTTTCTTACATGGGGCCCTCCCAGCGGCCCCTCTACACTGCCATCTCTGACCCCAGCC
CCTCGGGGCCCCAATCCCACAGTCCCACACACTGGGAGCAGCCAGTATATACGACTCTATCCCGACCT


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG207447 representing BC018551
Red=Cloning site Green=Tags(s)

MAEEQDLSEVELSPVGSEEPRCLSPGSAPSLGPDGGGGGSGLRASPGPGELGKVKKEQQDGEADDDKFPV
CIREAVSQVLSGYDWTLVPMPVRVNGASKSKPHVKRPMNAFMVWAQAARRKLADQYPHLHNAELSKTLGK
LWRLLNESDKRPFIEEAERLRMQHKKDHPDYKYQPRRRKNGKAAQGEAECPGGEAEQGGAAAIQAHYKSA
HLDHRHPEEGSPMSDGNPEHPSGQSHGPPTPPTTPKTELQSGKADPKRDGRSLGEGGKPHIDFGNVDIGE
ISHEVMSNMETFDVTELDQYLPPNGHPGHVGSYSAAGYGLGSALAVASGHSAWISKPPGVALPTVSPPGV
DAKAQVKTETTGPQGPPHYTDQPSTSQIAYTSLSLPHYGSAFPSISRPQFDYSDHQPSGPYYGHAGQASG
LYSAFSYMGPSQRPLYTAISDPSPSGPQSHSPTHWEQPVYTTLSRP

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC018551
ORF Size 1400 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC018551, AAH18551
RefSeq Size 2554 bp
RefSeq ORF 1400 bp
Locus ID 20665
Gene Summary Transcription factor that plays a central role in developing and mature glia (PubMed:24204311, PubMed:27532821). Specifically activates expression of myelin genes, during oligodendrocyte (OL) maturation, such as DUSP15 and MYRF, thereby playing a central role in oligodendrocyte maturation and CNS myelination (PubMed:24204311, PubMed:27532821). Once induced, MYRF cooperates with SOX10 to implement the myelination program (PubMed:24204311). Transcriptional activator of MITF, acting synergistically with PAX3 (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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