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Micu2 (NM_028643) Mouse Tagged ORF Clone
CAT＃: MG206892
- TrueORF®
Efha1 (tGFP-tagged) - Mouse EF hand domain family A1 (Efha1)
ORF Plasmid: DDK
"NM_028643" in other vectors (4)
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CNY 3,140.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 1110008L20Rik; 4833427E09Rik; AU041871; Efha1; Smhs2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG206892 representing NM_028643
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGGCTGCGGGAAGAAGCGCTTGGCTGGCGGCCTGGGGCGGAAGGCTGCGGCGCGGGCTCGCGG CCGGCCGACGAGCTGTGCCGACTCGCGGCCCTCTCGCAGCGGCTGTGGCCGGGGTAGCCCTGGCAGGCGC AGGAGCGGCATGGCATCACGGCCGCGTGAAAGCAGCCGCGCGCGAGGGCTCCCGAACGGTGTCGGCGCAG AAAAATTATTTGGGACCAATAGAGAAACTTTCTCTCCGCAAACAGCGGTTCATGCAGTTTTCATCCCTGG AGCACGACGGAGAGTATTACATGACACCCCGAGACTTCCTCTTCTCAGTCATGTTTGAGCAAGTGGAGCG TAAAACACTGGTCAAGAAGCTGGCAAAAAAGGATATCGAGGATGTACTGTCAGGAATCCAAACAGCACGC TGTGGATCAACATTTTTTAGAGACCTGGGTGATAAAGGGGTAATTTCATATACCGAGTATCTTTTCTTGC TTACAATCCTCACAAAACCTCACTCTGGGTTCCATGTTGCTTTTAAAATGCTGGATGTGGATGGGAATGA GATGATCGAGAGGAAGGAGTTTGTTAAGCTGCAGAAGATCATAAGTAAACAAGATGGCTTCAAGACAGTG AAGACTAATGAGACAGAATACCAGGACCCAACAGTGAAAGAGCCTGGCGTTAACACAACCCTTCAAGTGC GTTTCTTTGGGAAAAGAGGAGAAAAAAAACTCCATTATAAAGAATTTCGAAGATTTATGGAAAATTTACA AACAGAAGTTCAAGAAATGGAATTCCTTCAGTTCTCTAAAGGCCTGAATTTCATGAGAAAAGAAGACTTT GCAGAATGGCTACTTTTCTTCACTAACACTGAAAATAAAGATATTTACTGGAGAAACGTGAGAGAAAAGT TGTCAGTAGGAGAGAGCATTAGTTTGGATGAGTTCAAGTCCTTTTGCCATTTTACAACGCATTTGGAAGA CTTTGCTATCGCCATGCAAATGTTTAGTTTAGCTCATCGCCCTGTGCGGCTGGCAGAGTTCAAGAGAGCT GTGAAGGTAGCGACTGGACAGGAGCTCTCGGACAATCTTCTGGACACCGTCTTCAAGATCTTTGACCTGG ACGGGGACGAGTGCCTCAGCCACGGCGAGTTCCTTGGGGTACTGAAAAACAGGATGCACCGAGGCTTATG GGTGTCACAGCAGCAGAGTGTGCAAGAATACTGGAAGTGTGTGAAGAAGGAAAGCATCAAGGGAGTAAAG GAAGCCTGGAGACAACAAGCCGGCAAGGGCCCCTTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG206892 representing NM_028643
Red=Cloning site Green=Tags(s) MAAAAGRSAWLAAWGGRLRRGLAAGRRAVPTRGPLAAAVAGVALAGAGAAWHHGRVKAAAREGSRTVSAQ KNYLGPIEKLSLRKQRFMQFSSLEHDGEYYMTPRDFLFSVMFEQVERKTLVKKLAKKDIEDVLSGIQTAR CGSTFFRDLGDKGVISYTEYLFLLTILTKPHSGFHVAFKMLDVDGNEMIERKEFVKLQKIISKQDGFKTV KTNETEYQDPTVKEPGVNTTLQVRFFGKRGEKKLHYKEFRRFMENLQTEVQEMEFLQFSKGLNFMRKEDF AEWLLFFTNTENKDIYWRNVREKLSVGESISLDEFKSFCHFTTHLEDFAIAMQMFSLAHRPVRLAEFKRA VKVATGQELSDNLLDTVFKIFDLDGDECLSHGEFLGVLKNRMHRGLWVSQQQSVQEYWKCVKKESIKGVK EAWRQQAGKGPF TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_028643 |
| ORF Size | 1296 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_028643.3 |
| RefSeq Size | 2333 bp |
| RefSeq ORF | 1299 bp |
| Locus ID | 68514 |
| UniProt ID | Q8CD10 |
| Gene Summary | Key regulator of mitochondrial calcium uniporter (MCU) required to limit calcium uptake by MCU when cytoplasmic calcium is low (PubMed:23409044, PubMed:24560927). MICU1 and MICU2 form a disulfide-linked heterodimer that stimulate and inhibit MCU activity, depending on the concentration of calcium (PubMed:24560927). MICU2 acts as a gatekeeper of MCU that senses calcium level via its EF-hand domains: prevents channel opening at resting Ca(2+), avoiding energy dissipation and cell-death triggering (PubMed:24560927).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC201768 | Micu2 (untagged) - Mouse EF hand domain family A1 (Efha1), (10ug) |
CNY 2,000.00 |
|
| MR206892 | Efha1 (Myc-DDK-tagged) - Mouse EF hand domain family A1 (Efha1) |
CNY 2,850.00 |
|
| MR206892L3 | Lenti ORF clone of Efha1 (Myc-DDK-tagged) - Mouse EF hand domain family A1 (Efha1) |
CNY 4,750.00 |
|
| MR206892L4 | Lenti ORF clone of Efha1 (mGFP-tagged) - Mouse EF hand domain family A1 (Efha1) |
CNY 4,750.00 |
