Dmrt2 (BC027669) Mouse Tagged ORF Clone

CAT#: MG204805

  • TrueORF®

Dmrt2 (tGFP-tagged) - Mouse doublesex and mab-3 related transcription factor 2 (cDNA clone MGC:41586 IMAGE:1248080)

ORF Plasmid: DDK tGFP



  "BC027669" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 2,850.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


Rabbit polyclonal anti-DMRT2 antibody
    • 100 ul

CNY 5,250.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms Terra
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG204805 representing BC027669
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGGAAGAGAAGAGCCTTTGCTGACAAGGAGTTGGAGAACATTATGCTGGAGAGAGAATATAAAGAGA
GGGAGATGTTAGAAACATCTCAAGCTGCTGCCTTATTCCTGCCCAACCGTATGGTGCCTGGACCCGAGTA
CAGTTCCTACAAAGGTACCTACAGCCCCACTGCAGGAGAGCTGCCGAGCAAGGACTTCTGTAACTTTTTA
CCTACCTGCCTTGATCTCACCATGCAGTATTCAGGGTCTGGGAATATGGAACTTATTTCTTCTAACGTCA
GTGTGGCCACAACTTACAGACAATATCCCCTGTCCTCACGATTTTTAGTTTGGCCCAAGTGCGGTCCCAT
TAGTGACACCCTTCTCTACCAGCAATATCTGTTAAATGCTACCACTTCTGTCCAAGCTCTGAAGCCGGGG
ACCGGCTGGGACTTGAAAGGAACCCGAGTTCAGGATGGGCTTAGCGCAGAGCAAGACATGATGCCACCCA
AACTGGAAGGCTCTCTGGTGCTGCCCCATCTGCCAGAGGTCCCAGCCTCGAGAACAGACCTTCAGGTTCA
CCAGGTGGTTCCAGAGAGGTCTGCCTTCTCCCCACCCGGTCGGAATTTCTCTCCCATTGTTGATATGGAC
TGCCTGGCAGCTCAAGGGCACGTCTTAACCAAGCTCAGCAAAGAAAACACTAGACCTTCTCTGCCACTTA
AAACTAATCCATTCCACTCAGTATTCCAGCAGACGCTCAGCGACAAATCAGGCCCTGAGTTGAACGCACC
ATTTGTCAAAGAAGCCTTTGAAGAGACCCCAAAGAAACACAGAGAGTGTTTGGTCAAGGAGAGCCAGAAG
TACACATTTACAATAGACAGATGCGCAAAAGACCTCTTTGTAGCCAAACAAGTTGGAACGAAACTTTCGG
CGAATGAGCCACTGTCGTTCTCTGTCGAATCTATTCTTAAGAGGCCTTCATCTGCCGTCACTCACGTCTC
TCAG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG204805 representing BC027669
Red=Cloning site Green=Tags(s)

MRKRRAFADKELENIMLEREYKEREMLETSQAAALFLPNRMVPGPEYSSYKGTYSPTAGELPSKDFCNFL
PTCLDLTMQYSGSGNMELISSNVSVATTYRQYPLSSRFLVWPKCGPISDTLLYQQYLLNATTSVQALKPG
TGWDLKGTRVQDGLSAEQDMMPPKLEGSLVLPHLPEVPASRTDLQVHQVVPERSAFSPPGRNFSPIVDMD
CLAAQGHVLTKLSKENTRPSLPLKTNPFHSVFQQTLSDKSGPELNAPFVKEAFEETPKKHRECLVKESQK
YTFTIDRCAKDLFVAKQVGTKLSANEPLSFSVESILKRPSSAVTHVSQ

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC027669
ORF Size 986 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC027669, AAH27669
RefSeq Size 1304 bp
RefSeq ORF 986 bp
Locus ID 226049
Gene Summary Transcriptional activator that directly regulates early activation of the myogenic determination gene MYF5 by binding in a sequence-specific manner to the early epaxial enhancer element of it. Involved in somitogenesis during embryogenesis and somite development and differentiation into sclerotome and dermomyotome. Required for the initiation and/or maintenance of proper organization of the sclerotome, dermomyotome and myotome. Is not required for sex determination and/or differentiation in embryonic development. Also not involved in symmetric somite formation and hence does not regulate the laterality pathway that controls left-right asymmetric organ positioning.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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