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Ranbp1 (NM_011239) Mouse Tagged ORF Clone
CAT＃: MG202097
- TrueORF®
Ranbp1 (tGFP-tagged) - Mouse RAN binding protein 1 (Ranbp1)
ORF Plasmid: DDK
"NM_011239" in other vectors (4)
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CNY 2,850.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Htf9a |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG202097 representing NM_011239
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCCGCCAAGGACAGTCACGAGGACCATGATACTTCCACAGAGAATGCAGATGAGTCCAACCACG ACCCCCAGTTCGAGCCAATAGTTTCTCTTCCCGAGCAAGAAATTAAAACGCTGGAGGAAGATGAAGAGGA ACTTTTTAAGATGCGTGCAAAGCTGTTCCGGTTTGCTTCAGAGAATGACCTCCCAGAATGGAAGGAGCGA GGCACTGGAGATGTCAAGCTTCTGAAGCACAAGGAGAAAGGGACCATCCGCCTTCTTATGAGGAGGGACA AAACCTTGAAGATATGCGCCAACCACTATATTACACCAATGATGGAGCTGAAGCCGAATGCTGGCAGTGA CCGAGCCTGGGTCTGGAATACCCACGCCGACTTTGCTGACGAGTGCCCCAAGCCTGAGCTGCTCGCCATC CGCTTCCTAAATGCTGAGAATGCACAAAAGTTCAAAACAAAGTTTGAAGAATGCAGGAAAGAAATTGAAG AGAGAGAAAAGAAAGGACCAGGCAAAAATGATAATGCCGAAAAGGTGGCCGAGAAGCTGGAAGCCCTTTC AGTGAGGGAGGCCAGAGAGGAGGCTGAAGAGAAGTCTGAGGAGAAACAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG202097 representing NM_011239
Red=Cloning site Green=Tags(s) MAAAKDSHEDHDTSTENADESNHDPQFEPIVSLPEQEIKTLEEDEEELFKMRAKLFRFASENDLPEWKER GTGDVKLLKHKEKGTIRLLMRRDKTLKICANHYITPMMELKPNAGSDRAWVWNTHADFADECPKPELLAI RFLNAENAQKFKTKFEECRKEIEEREKKGPGKNDNAEKVAEKLEALSVREAREEAEEKSEEKQ TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_011239 |
| ORF Size | 609 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_011239.2, NP_035369.2 |
| RefSeq Size | 852 bp |
| RefSeq ORF | 612 bp |
| Locus ID | 19385 |
| UniProt ID | P34022 |
| Gene Summary | Plays a role in RAN-dependent nucleocytoplasmic transport. Alleviates the TNPO1-dependent inhibition of RAN GTPase activity and mediates the dissociation of RAN from proteins involved in transport into the nucleus (PubMed:9428644). Induces a conformation change in the complex formed by XPO1 and RAN that triggers the release of the nuclear export signal of cargo proteins (By similarity). Promotes the disassembly of the complex formed by RAN and importin beta. Promotes dissociation of RAN from a complex with KPNA2 and CSE1L (PubMed:9428644). Required for normal mitotic spindle assembly and normal progress through mitosis via its effect on RAN (By similarity). Does not increase the RAN GTPase activity by itself, but increases GTP hydrolysis mediated by RANGAP1 (PubMed:9428644). Inhibits RCC1-dependent exchange of RAN-bound GDP by GTP (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC209292 | Ranbp1 (untagged) - Mouse RAN binding protein 1 (Ranbp1), (10ug) |
CNY 3,990.00 |
|
| MR202097 | Ranbp1 (Myc-DDK-tagged) - Mouse RAN binding protein 1 (Ranbp1) |
CNY 2,400.00 |
|
| MR202097L3 | Lenti ORF clone of Ranbp1 (Myc-DDK-tagged) - Mouse RAN binding protein 1 (Ranbp1) |
CNY 4,750.00 |
|
| MR202097L4 | Lenti ORF clone of Ranbp1 (mGFP-tagged) - Mouse RAN binding protein 1 (Ranbp1) |
CNY 4,750.00 |
