Cacna1c (NM_001290335) Mouse Untagged Clone

CAT#: MC229677

Cacna1c (untagged) - Mouse calcium channel, voltage-dependent, L type, alpha 1C subunit (Cacna1c), transcript variant 11



  "NM_001290335" in other vectors (1)

CNY 18,050.00


货期*
8周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms Cav1.2; Cchl1a1; D930026N18Rik; MBC; MELC-CC
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC229677 representing NM_001290335
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGCCTCTGCTGCACATTGCCCTTCTTGTGCTCTTCGTCATCATCATTTATGCTATTATCGGCCTGG
AGCTCTTCATGGGAAAGATGCACAAGACCTGCTACAACCAGGAGGGCATAATAGATGTTCCGGCAGAAGA
GGATCCTTCCCCTTGTGCTTTGGAGACAGGCCATGGGCGACAGTGTCAGAACGGGACCGTGTGCAAACCC
GGGTGGGATGGGCCCAAGCACGGCATCACCAACTTCGACAACTTCGCCTTCGCCATGCTGACGGTGTTCC
AGTGTATCACCATGGAGGGCTGGACAGACGTGCTGTACTGGGTCAATGATGCCGTAGGAAGGGACTGGCC
CTGGATCTATTTTGTAACACTAATCATCATAGGGTCATTTTTTGTACTTAACTTGGTTCTCGGTGTTCTT
AGCGGGGAGTTTTCCAAAGAGAGGGAGAAAGCCAAAGCCCGAGGAGATTTCCAGAAGCTTCGAGAGAAGC
AGCAACTAGAAGAAGATCTCAAAGGCTACCTGGACTGGATCACCCAGGCAGAAGACATTGACCCCGAGAA
TGAGGACGAGGGCATGGATGAAGACAAGCCTCGAAACAGAGGCGCTCCAGCGGGCTTGCATGATCAGAAG
AAAGGGAAGTTTGCTTGGTTTAGTCACTCTACAGAAACCCATGTGAGCATGCCCACAAGTGAGACTGAGT
CTGTCAACACTGAAAACGTGGCTGGAGGTGACATCGAGGGAGAAAACTGTGGAGCCAGGCTTGCCCATCG
GATCTCCAAATCCAAATTCAGCCGCTACTGGCGCAGGTGGAATCGATTCTGCAGAAGAAAATGCCGTGCA
GCAGTTAAGTCCAACGTCTTCTACTGGCTCGTGATCTTCCTGGTGTTCCTCAACACCCTCACCATTGCCT
CCGAACATTACAACCAGCCTCACTGGCTCACAGAAGTGCAAGACACAGCCAATAAAGCCCTCCTGGCCCT
TTTCACTGCAGAAATGCTCCTGAAGATGTACAGCCTGGGTCTTCAGGCCTATTTTGTGTCCCTCTTCAAC
CGCTTTGACTGTTTCATTGTGTGTGGGGGCATCCTGGAGACCATCCTGGTGGAGACGAAGATCATGTCTC
CCCTGGGCATCTCTGTGCTGAGATGTGTGCGGTTGCTCAGGATCTTCAAGATCACCAGGTACTGGAATTC
CTTGAGCAACCTTGTGGCATCCTTGCTGAACTCAGTGCGCTCCATTGCCTCCCTGCTGCTGCTCCTCTTC
CTCTTCATCATCATCTTCTCCCTCCTGGGGATGCAGCTCTTTGGAGGGAAGTTCAATTTCGATGAGATGC
AGACCCGTAGGAGCACGTTCGATAACTTCCCGCAGTCTCTCCTCACTGTGTTTCAGATCCTGACCGGGGA
GGACTGGAATTCGGTGATGTATGATGGGATCATGGCTTATGGCGGCCCCTCTTTTCCAGGGATGTTAGTC
TGTATTTACTTCATCATCCTCTTCATCTGTGGAAATTATATCCTACTGAATGTGTTCTTGGCCATTGCGG
TGGACAACCTGGCTGATGCGGAGAGCCTGACCTCAGCCCAAAAGGAGGAGGAAGAAGAGAAGGAGAGGAA
GAAGCTGGCCAGGACTGCCAGCCCAGAAAAGAAACAGGAGGTGATGGAGAAGCCAGCCGTGGAGGAGAGC
AAAGAGGAGAAAATTGAACTGAAATCCATTACAGCCGATGGAGAATCCCCACCCACTACCAAGATCAACA
TGGATGACCTCCAGCCCAGTGAAAACGAGGATAAGAGTCCCCACTCCAACCCAGACACTGCAGGGGAAGA
GGATGAAGAGGAGCCAGAGATGCCTGTGGGGCCACGCCCCCGGCCCCTGTCTGAGCTGCACCTTAAGGAA
AAGGCAGTTCCCATGCCGGAAGCCAGTGCATTTTTCATCTTCAGCCCAAACAACAGGTTCCGCCTGCAGT
GCCACCGTATTGTCAATGACACGATCTTCACCAACCTCATCCTCTTCTTCATTCTGCTCAGCAGCATCTC
TCTGGCTGCTGAGGACCCCGTCCAGCACACCTCCTTCAGGAACCATATGACTGCTTACGGGGCTTTCCTG
CACAAGGGCTCTTTCTGCCGAAACTACTTCAATATCCTGGACCTGCTGGTGGTTAGCGTGTCCCTCATCT
CCTTTGGCATCCAGTCCAGCGCGATCAACGTTGTGAAGATTTTACGAGTGCTGCGAGTCCTCAGACCCCT
GAGGGCCATCAACAGGGCCAAGGGGCTAAAGCATGTGGTTCAGTGCGTGTTTGTGGCCATCCGGACCATC
GGGAACATCGTAATTGTCACCACTCTGCTGCAGTTCATGTTCGCCTGCATTGGGGTCCAGCTCTTCAAGG
GAAAGCTCTATACCTGTTCGGATAGTTCTAAACAGACGGAGGCAGAATGCAAGGGTAACTATATCACATA
CAAAGATGGAGAGGTCGATCACCCCATTATCCAGCCTCGAAGCTGGGAGAACAGCAAGTTTGACTTTGAC
AATGTTTTGGCAGCCATGATGGCTCTCTTCACCGTCTCCACCTTCGAAGGGTGGCCAGAGCTGCTGTACC
GCTCCATTGACTCCCACACAGAAGACAAGGGCCCCATCTACAACTACCGTGTGGAGATCTCCATCTTCTT
CATCATCTATATCATCATCATTGCCTTCTTCATGATGAACATCTTCGTGGGTTTCGTCATTGTCACCTTC
CAGGAGCAGGGGGAACAAGAGTACAAGAACTGTGAGCTGGACAAGAACCAGAGACAATGTGTGGAATATG
CCCTCAAGGCCCGACCCTTGCGAAGGTACATCCCCAAGAACCAGCACCAGTACAAAGTGTGGTACGTGGT
CAACTCTACCTACTTCGAGTATCTGATGTTCGTTCTCATCCTGCTCAACACCATCTGCCTGGCCATGCAG
CACTATGGCCAGAGCTGCCTCTTCAAAATCGCCATGAATATACTCAACATGCTTTTCACCGGCCTCTTCA
CAGTGGAGATGATCCTGAAGCTCATTGCCTTCAAACCCAAGGGTTACTTTAGTGATCCCTGGAATGTTTT
TGACTTCCTCATCGTCATTGGGAGCATAATTGATGTCATTCTCAGTGAGACTAATAGTGCAGAGGAGAAC
TCCCGCATCTCCATCACCTTCTTCCGCCTCTTCCGGGTCATGCGCCTGGTGAAGCTGCTGAGCCGCGGGG
AAGGCATCCGAACCCTGCTGTGGACCTTCATCAAGTCCTTCCAGGCTCTGCCCTATGTGGCTCTTTTGAT
TGTGATGCTGTTCTTTATCTATGCAGTGATTGGGATGCAGGTGTTTGGGAAGATTGCCCTGAATGACACC
ACAGAGATCAATCGGAACAACAACTTCCAGACGTTCCCCCAGGCTGTGTTACTGCTGTTCAGGTGTGCCA
CCGGAGAGGCCTGGCAGGACATCATGCTGGCCTGCATGCCAGGCAAGAAGTGTGCCCCAGAGTCTGAGCC
CAGCAACAGCACGGAAGGGGAGACCCCCTGTGGCAGCAGCTTTGCTGTCTTCTACTTCATCAGCTTCTAC
ATGCTCTGTGCCTTCCTGATCATCAACCTCTTTGTAGCTGTTATCATGGACAACTTTGACTACCTGACTA
GGGATTGGTCTATCCTCGGTCCCCATCACCTGGATGAATTCAAGAGAATCTGGGCCGAGTATGACCCTGA
AGCCAAGGGTCGGATCAAACACTTGGATGTGGTGACCCTCCTCCGTCGAATTCAGCCCCCACTGGGTTTT
GGGAAATTGTGTCCTCACCGTGTGGCCTGCAAACGCCTGGTGTCCATGAACATGCCTCTGAACAGCGATG
GCACAGTCATGTTCAATGCTACCCTGTTTGCCCTCGTCAGGACAGCCCTGAGGATCAAAACAGAAGGGAA
CCTAGAGCAAGCCAATGAGGAGCTTCGGGCCATCATCAAGAAAATCTGGAAGAGGACTAGCATGAAGCTG
TTGGACCAGGTGGTGCCCCCTGCAGGCGATGACGAGGTCACAGTGGGCAAGTTCTATGCCACCTTCCTGA
TCCAAGAGTACTTCAGGAAATTCAAGAAGCGAAAAGAGCAGGGGCTGGTGGGCAAGCCCTCACAAAGGAA
TGCACTGTCCCTCCAGGCTGGCTTGCGCACCTTGCATGACATTGGGCCTGAGATCCGGCGGGCCATCTCT
GGGGATCTGACTGCTGAGGAGGAGTTGGACAAGGCTATGAAGGAGGCGGTGTCTGCTGCCTCCGAAGATG
ACATCTTCAGGAGGGCTGGAGGCCTGTTCGGCAACCATGTCACCTACTATCAGAGTGACAGCAGGGGCAA
CTTTCCTCAGACGTTCGCCACCCAGCGCCCACTGCACATCAACAAGACAGGGAACAACCAAGCTGACACT
GAGTCACCGTCCCATGAGAAGCTGGTGGACTCCACGTTCACCCCCAGCAGCTACTCATCCACGGGCTCCA
ATGCCAACATCAACAATGCCAACAACACTGCCCTGGGCCGCTTCCCCCATCCCGCTGGCTACTCCAGCAC
GGTCAGCACTGTGGAGGGCCATGGGCCTCCCTTGTCCCCTGCTGTCCGAGTACAGGAGGCAGCATGGAAG
CTCAGCTCTAAGAGGTGCCACTCCCGAGAGAGCCAGGGAGCCACGGTGAATCAGGAGATATTTCCAGATG
AGACCCGCAGCGTAAGGATGAGTGAAGAAGCCGAGTACTGCAGTGAGCCCAGCCTGCTCTCCACAGATAT
GTTCTCCTACCAGGAAGATGAACACCGACAACTGACCTGCCCAGAGGAGGACAAGAGGGAGATCCAGCCA
TCTCCAAAGAGGAGTTTCCTTCGCTCTGCCTCTCTAGGTCGAAGGGCCTCCTTCCATCTGGAATGTCTAA
AGCGACAAAAGGATCAAGGGGGAGACATCTCTCAGAAGACAGCCTTGCCCTTGCATCTGGTTCATCATCA
GGCATTGGCAGTGGCAGGCTTGAGCCCCCTCCTGCAGAGAAGCCATTCTCCTACCACATTCCCCAGGCCG
TGCCCCACACCCCCTGTCACTCCAGGCAGCCGGGGCAGACCCCTACGGCCCATCCCTACCCTACGGCTGG
AGGGGGCAGAGTCCAGCGAGAAACTCAACAGCAGCTTCCCATCCATCCACTGCAGCTCCTGGTCTGAGGA
GACGACAGCCTGTAGTGGGAGCAGCAGCATGGCCCGGAGAGCCCGGCCCGTCTCCCTCACCGTGCCCAGC
CAGGCTGGAGCTCCAGGGAGACAGTTCCATGGCAGTGCCAGCAGCCTGGTGGAAGCGGTCTTGATTTCAG
AAGGACTGGGACAGTTTGCTCAAGATCCCAAGTTCATCGAGGTCACCACCCAGGAGCTGGCTGACGCCTG
CGACATGACAATAGAGGAGATGGAGAACGCCGCAGACAACATCCTCAGTGGGGGCGCCCAGCAGAGCCCC
AACGGCACCCTCTTACCTTTTGTGAACTGCAGGGACCCGGGGCAGGACAGGGCTGTGGCCCCAGAGGACG
AGAGCTGCGCATATGCCCTGGGGCGAGGCCGGAGCGAGGAGGCGCTCGCGGACAGCAGGTCCTACGTCAG
CAACCTGTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001290335
Insert Size 5610 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001290335.1, NP_001277264.1
RefSeq Size 12656 bp
RefSeq ORF 5610 bp
Locus ID 12288
Gene Summary Pore-forming, alpha-1C subunit of the voltage-gated calcium channel that gives rise to L-type calcium currents (PubMed:14609949, PubMed:18586882, PubMed:21216955, PubMed:25368181, PubMed:28119464). Mediates influx of calcium ions into the cytoplasm, and thereby triggers calcium release from the sarcoplasm (By similarity). Plays an important role in excitation-contraction coupling in the heart. Required for normal heart development and normal regulation of heart rhythm (PubMed:21216955). Required for normal contraction of smooth muscle cells in blood vessels and in the intestine. Essential for normal blood pressure regulation via its role in the contraction of arterial smooth muscle cells (PubMed:14609949, PubMed:28119464). Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group (Probable).[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (11) contains an alternate 5'-terminal exon, lacks part of the 5' coding region, uses a downstream start codon, and has multiple coding region differences, compared to variant 1. The encoded isoform (11) is shorter, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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