Cacna1c (NM_001290335) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Cav1.2; Cchl1a1; D930026N18Rik; MBC; MELC-CC
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229677 representing NM_001290335 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGCCTCTGCTGCACATTGCCCTTCTTGTGCTCTTCGTCATCATCATTTATGCTATTATCGGCCTGG AGCTCTTCATGGGAAAGATGCACAAGACCTGCTACAACCAGGAGGGCATAATAGATGTTCCGGCAGAAGA GGATCCTTCCCCTTGTGCTTTGGAGACAGGCCATGGGCGACAGTGTCAGAACGGGACCGTGTGCAAACCC GGGTGGGATGGGCCCAAGCACGGCATCACCAACTTCGACAACTTCGCCTTCGCCATGCTGACGGTGTTCC AGTGTATCACCATGGAGGGCTGGACAGACGTGCTGTACTGGGTCAATGATGCCGTAGGAAGGGACTGGCC CTGGATCTATTTTGTAACACTAATCATCATAGGGTCATTTTTTGTACTTAACTTGGTTCTCGGTGTTCTT AGCGGGGAGTTTTCCAAAGAGAGGGAGAAAGCCAAAGCCCGAGGAGATTTCCAGAAGCTTCGAGAGAAGC AGCAACTAGAAGAAGATCTCAAAGGCTACCTGGACTGGATCACCCAGGCAGAAGACATTGACCCCGAGAA TGAGGACGAGGGCATGGATGAAGACAAGCCTCGAAACAGAGGCGCTCCAGCGGGCTTGCATGATCAGAAG AAAGGGAAGTTTGCTTGGTTTAGTCACTCTACAGAAACCCATGTGAGCATGCCCACAAGTGAGACTGAGT CTGTCAACACTGAAAACGTGGCTGGAGGTGACATCGAGGGAGAAAACTGTGGAGCCAGGCTTGCCCATCG GATCTCCAAATCCAAATTCAGCCGCTACTGGCGCAGGTGGAATCGATTCTGCAGAAGAAAATGCCGTGCA GCAGTTAAGTCCAACGTCTTCTACTGGCTCGTGATCTTCCTGGTGTTCCTCAACACCCTCACCATTGCCT CCGAACATTACAACCAGCCTCACTGGCTCACAGAAGTGCAAGACACAGCCAATAAAGCCCTCCTGGCCCT TTTCACTGCAGAAATGCTCCTGAAGATGTACAGCCTGGGTCTTCAGGCCTATTTTGTGTCCCTCTTCAAC CGCTTTGACTGTTTCATTGTGTGTGGGGGCATCCTGGAGACCATCCTGGTGGAGACGAAGATCATGTCTC CCCTGGGCATCTCTGTGCTGAGATGTGTGCGGTTGCTCAGGATCTTCAAGATCACCAGGTACTGGAATTC CTTGAGCAACCTTGTGGCATCCTTGCTGAACTCAGTGCGCTCCATTGCCTCCCTGCTGCTGCTCCTCTTC CTCTTCATCATCATCTTCTCCCTCCTGGGGATGCAGCTCTTTGGAGGGAAGTTCAATTTCGATGAGATGC AGACCCGTAGGAGCACGTTCGATAACTTCCCGCAGTCTCTCCTCACTGTGTTTCAGATCCTGACCGGGGA GGACTGGAATTCGGTGATGTATGATGGGATCATGGCTTATGGCGGCCCCTCTTTTCCAGGGATGTTAGTC TGTATTTACTTCATCATCCTCTTCATCTGTGGAAATTATATCCTACTGAATGTGTTCTTGGCCATTGCGG TGGACAACCTGGCTGATGCGGAGAGCCTGACCTCAGCCCAAAAGGAGGAGGAAGAAGAGAAGGAGAGGAA GAAGCTGGCCAGGACTGCCAGCCCAGAAAAGAAACAGGAGGTGATGGAGAAGCCAGCCGTGGAGGAGAGC AAAGAGGAGAAAATTGAACTGAAATCCATTACAGCCGATGGAGAATCCCCACCCACTACCAAGATCAACA TGGATGACCTCCAGCCCAGTGAAAACGAGGATAAGAGTCCCCACTCCAACCCAGACACTGCAGGGGAAGA GGATGAAGAGGAGCCAGAGATGCCTGTGGGGCCACGCCCCCGGCCCCTGTCTGAGCTGCACCTTAAGGAA AAGGCAGTTCCCATGCCGGAAGCCAGTGCATTTTTCATCTTCAGCCCAAACAACAGGTTCCGCCTGCAGT GCCACCGTATTGTCAATGACACGATCTTCACCAACCTCATCCTCTTCTTCATTCTGCTCAGCAGCATCTC TCTGGCTGCTGAGGACCCCGTCCAGCACACCTCCTTCAGGAACCATATGACTGCTTACGGGGCTTTCCTG CACAAGGGCTCTTTCTGCCGAAACTACTTCAATATCCTGGACCTGCTGGTGGTTAGCGTGTCCCTCATCT CCTTTGGCATCCAGTCCAGCGCGATCAACGTTGTGAAGATTTTACGAGTGCTGCGAGTCCTCAGACCCCT GAGGGCCATCAACAGGGCCAAGGGGCTAAAGCATGTGGTTCAGTGCGTGTTTGTGGCCATCCGGACCATC GGGAACATCGTAATTGTCACCACTCTGCTGCAGTTCATGTTCGCCTGCATTGGGGTCCAGCTCTTCAAGG GAAAGCTCTATACCTGTTCGGATAGTTCTAAACAGACGGAGGCAGAATGCAAGGGTAACTATATCACATA CAAAGATGGAGAGGTCGATCACCCCATTATCCAGCCTCGAAGCTGGGAGAACAGCAAGTTTGACTTTGAC AATGTTTTGGCAGCCATGATGGCTCTCTTCACCGTCTCCACCTTCGAAGGGTGGCCAGAGCTGCTGTACC GCTCCATTGACTCCCACACAGAAGACAAGGGCCCCATCTACAACTACCGTGTGGAGATCTCCATCTTCTT CATCATCTATATCATCATCATTGCCTTCTTCATGATGAACATCTTCGTGGGTTTCGTCATTGTCACCTTC CAGGAGCAGGGGGAACAAGAGTACAAGAACTGTGAGCTGGACAAGAACCAGAGACAATGTGTGGAATATG CCCTCAAGGCCCGACCCTTGCGAAGGTACATCCCCAAGAACCAGCACCAGTACAAAGTGTGGTACGTGGT CAACTCTACCTACTTCGAGTATCTGATGTTCGTTCTCATCCTGCTCAACACCATCTGCCTGGCCATGCAG CACTATGGCCAGAGCTGCCTCTTCAAAATCGCCATGAATATACTCAACATGCTTTTCACCGGCCTCTTCA CAGTGGAGATGATCCTGAAGCTCATTGCCTTCAAACCCAAGGGTTACTTTAGTGATCCCTGGAATGTTTT TGACTTCCTCATCGTCATTGGGAGCATAATTGATGTCATTCTCAGTGAGACTAATAGTGCAGAGGAGAAC TCCCGCATCTCCATCACCTTCTTCCGCCTCTTCCGGGTCATGCGCCTGGTGAAGCTGCTGAGCCGCGGGG AAGGCATCCGAACCCTGCTGTGGACCTTCATCAAGTCCTTCCAGGCTCTGCCCTATGTGGCTCTTTTGAT TGTGATGCTGTTCTTTATCTATGCAGTGATTGGGATGCAGGTGTTTGGGAAGATTGCCCTGAATGACACC ACAGAGATCAATCGGAACAACAACTTCCAGACGTTCCCCCAGGCTGTGTTACTGCTGTTCAGGTGTGCCA CCGGAGAGGCCTGGCAGGACATCATGCTGGCCTGCATGCCAGGCAAGAAGTGTGCCCCAGAGTCTGAGCC CAGCAACAGCACGGAAGGGGAGACCCCCTGTGGCAGCAGCTTTGCTGTCTTCTACTTCATCAGCTTCTAC ATGCTCTGTGCCTTCCTGATCATCAACCTCTTTGTAGCTGTTATCATGGACAACTTTGACTACCTGACTA GGGATTGGTCTATCCTCGGTCCCCATCACCTGGATGAATTCAAGAGAATCTGGGCCGAGTATGACCCTGA AGCCAAGGGTCGGATCAAACACTTGGATGTGGTGACCCTCCTCCGTCGAATTCAGCCCCCACTGGGTTTT GGGAAATTGTGTCCTCACCGTGTGGCCTGCAAACGCCTGGTGTCCATGAACATGCCTCTGAACAGCGATG GCACAGTCATGTTCAATGCTACCCTGTTTGCCCTCGTCAGGACAGCCCTGAGGATCAAAACAGAAGGGAA CCTAGAGCAAGCCAATGAGGAGCTTCGGGCCATCATCAAGAAAATCTGGAAGAGGACTAGCATGAAGCTG TTGGACCAGGTGGTGCCCCCTGCAGGCGATGACGAGGTCACAGTGGGCAAGTTCTATGCCACCTTCCTGA TCCAAGAGTACTTCAGGAAATTCAAGAAGCGAAAAGAGCAGGGGCTGGTGGGCAAGCCCTCACAAAGGAA TGCACTGTCCCTCCAGGCTGGCTTGCGCACCTTGCATGACATTGGGCCTGAGATCCGGCGGGCCATCTCT GGGGATCTGACTGCTGAGGAGGAGTTGGACAAGGCTATGAAGGAGGCGGTGTCTGCTGCCTCCGAAGATG ACATCTTCAGGAGGGCTGGAGGCCTGTTCGGCAACCATGTCACCTACTATCAGAGTGACAGCAGGGGCAA CTTTCCTCAGACGTTCGCCACCCAGCGCCCACTGCACATCAACAAGACAGGGAACAACCAAGCTGACACT GAGTCACCGTCCCATGAGAAGCTGGTGGACTCCACGTTCACCCCCAGCAGCTACTCATCCACGGGCTCCA ATGCCAACATCAACAATGCCAACAACACTGCCCTGGGCCGCTTCCCCCATCCCGCTGGCTACTCCAGCAC GGTCAGCACTGTGGAGGGCCATGGGCCTCCCTTGTCCCCTGCTGTCCGAGTACAGGAGGCAGCATGGAAG CTCAGCTCTAAGAGGTGCCACTCCCGAGAGAGCCAGGGAGCCACGGTGAATCAGGAGATATTTCCAGATG AGACCCGCAGCGTAAGGATGAGTGAAGAAGCCGAGTACTGCAGTGAGCCCAGCCTGCTCTCCACAGATAT GTTCTCCTACCAGGAAGATGAACACCGACAACTGACCTGCCCAGAGGAGGACAAGAGGGAGATCCAGCCA TCTCCAAAGAGGAGTTTCCTTCGCTCTGCCTCTCTAGGTCGAAGGGCCTCCTTCCATCTGGAATGTCTAA AGCGACAAAAGGATCAAGGGGGAGACATCTCTCAGAAGACAGCCTTGCCCTTGCATCTGGTTCATCATCA GGCATTGGCAGTGGCAGGCTTGAGCCCCCTCCTGCAGAGAAGCCATTCTCCTACCACATTCCCCAGGCCG TGCCCCACACCCCCTGTCACTCCAGGCAGCCGGGGCAGACCCCTACGGCCCATCCCTACCCTACGGCTGG AGGGGGCAGAGTCCAGCGAGAAACTCAACAGCAGCTTCCCATCCATCCACTGCAGCTCCTGGTCTGAGGA GACGACAGCCTGTAGTGGGAGCAGCAGCATGGCCCGGAGAGCCCGGCCCGTCTCCCTCACCGTGCCCAGC CAGGCTGGAGCTCCAGGGAGACAGTTCCATGGCAGTGCCAGCAGCCTGGTGGAAGCGGTCTTGATTTCAG AAGGACTGGGACAGTTTGCTCAAGATCCCAAGTTCATCGAGGTCACCACCCAGGAGCTGGCTGACGCCTG CGACATGACAATAGAGGAGATGGAGAACGCCGCAGACAACATCCTCAGTGGGGGCGCCCAGCAGAGCCCC AACGGCACCCTCTTACCTTTTGTGAACTGCAGGGACCCGGGGCAGGACAGGGCTGTGGCCCCAGAGGACG AGAGCTGCGCATATGCCCTGGGGCGAGGCCGGAGCGAGGAGGCGCTCGCGGACAGCAGGTCCTACGTCAG CAACCTGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001290335
Insert Size	5610 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001290335.1, NP_001277264.1
RefSeq Size	12656 bp
RefSeq ORF	5610 bp
Locus ID	12288
Gene Summary	Pore-forming, alpha-1C subunit of the voltage-gated calcium channel that gives rise to L-type calcium currents (PubMed:14609949, PubMed:18586882, PubMed:21216955, PubMed:25368181, PubMed:28119464). Mediates influx of calcium ions into the cytoplasm, and thereby triggers calcium release from the sarcoplasm (By similarity). Plays an important role in excitation-contraction coupling in the heart. Required for normal heart development and normal regulation of heart rhythm (PubMed:21216955). Required for normal contraction of smooth muscle cells in blood vessels and in the intestine. Essential for normal blood pressure regulation via its role in the contraction of arterial smooth muscle cells (PubMed:14609949, PubMed:28119464). Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group (Probable).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (11) contains an alternate 5'-terminal exon, lacks part of the 5' coding region, uses a downstream start codon, and has multiple coding region differences, compared to variant 1. The encoded isoform (11) is shorter, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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