Cacna1c (NM_001290335) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Cav1.2; Cchl1a1; D930026N18Rik; MBC; MELC-CC
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231888 representing NM_001290335 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGCCTCTGCTGCACATTGCCCTTCTTGTGCTCTTCGTCATCATCATTTATGCTATTATCGGCCTGG AGCTCTTCATGGGAAAGATGCACAAGACCTGCTACAACCAGGAGGGCATAATAGATGTTCCGGCAGAAGA GGATCCTTCCCCTTGTGCTTTGGAGACAGGCCATGGGCGACAGTGTCAGAACGGGACCGTGTGCAAACCC GGGTGGGATGGGCCCAAGCACGGCATCACCAACTTCGACAACTTCGCCTTCGCCATGCTGACGGTGTTCC AGTGTATCACCATGGAGGGCTGGACAGACGTGCTGTACTGGGTCAATGATGCCGTAGGAAGGGACTGGCC CTGGATCTATTTTGTAACACTAATCATCATAGGGTCATTTTTTGTACTTAACTTGGTTCTCGGTGTTCTT AGCGGGGAGTTTTCCAAAGAGAGGGAGAAAGCCAAAGCCCGAGGAGATTTCCAGAAGCTTCGAGAGAAGC AGCAACTAGAAGAAGATCTCAAAGGCTACCTGGACTGGATCACCCAGGCAGAAGACATTGACCCCGAGAA TGAGGACGAGGGCATGGATGAAGACAAGCCTCGAAACAGAGGCGCTCCAGCGGGCTTGCATGATCAGAAG AAAGGGAAGTTTGCTTGGTTTAGTCACTCTACAGAAACCCATGTGAGCATGCCCACAAGTGAGACTGAGT CTGTCAACACTGAAAACGTGGCTGGAGGTGACATCGAGGGAGAAAACTGTGGAGCCAGGCTTGCCCATCG GATCTCCAAATCCAAATTCAGCCGCTACTGGCGCAGGTGGAATCGATTCTGCAGAAGAAAATGCCGTGCA GCAGTTAAGTCCAACGTCTTCTACTGGCTCGTGATCTTCCTGGTGTTCCTCAACACCCTCACCATTGCCT CCGAACATTACAACCAGCCTCACTGGCTCACAGAAGTGCAAGACACAGCCAATAAAGCCCTCCTGGCCCT TTTCACTGCAGAAATGCTCCTGAAGATGTACAGCCTGGGTCTTCAGGCCTATTTTGTGTCCCTCTTCAAC CGCTTTGACTGTTTCATTGTGTGTGGGGGCATCCTGGAGACCATCCTGGTGGAGACGAAGATCATGTCTC CCCTGGGCATCTCTGTGCTGAGATGTGTGCGGTTGCTCAGGATCTTCAAGATCACCAGGTACTGGAATTC CTTGAGCAACCTTGTGGCATCCTTGCTGAACTCAGTGCGCTCCATTGCCTCCCTGCTGCTGCTCCTCTTC CTCTTCATCATCATCTTCTCCCTCCTGGGGATGCAGCTCTTTGGAGGGAAGTTCAATTTCGATGAGATGC AGACCCGTAGGAGCACGTTCGATAACTTCCCGCAGTCTCTCCTCACTGTGTTTCAGATCCTGACCGGGGA GGACTGGAATTCGGTGATGTATGATGGGATCATGGCTTATGGCGGCCCCTCTTTTCCAGGGATGTTAGTC TGTATTTACTTCATCATCCTCTTCATCTGTGGAAATTATATCCTACTGAATGTGTTCTTGGCCATTGCGG TGGACAACCTGGCTGATGCGGAGAGCCTGACCTCAGCCCAAAAGGAGGAGGAAGAAGAGAAGGAGAGGAA GAAGCTGGCCAGGACTGCCAGCCCAGAAAAGAAACAGGAGGTGATGGAGAAGCCAGCCGTGGAGGAGAGC AAAGAGGAGAAAATTGAACTGAAATCCATTACAGCCGATGGAGAATCCCCACCCACTACCAAGATCAACA TGGATGACCTCCAGCCCAGTGAAAACGAGGATAAGAGTCCCCACTCCAACCCAGACACTGCAGGGGAAGA GGATGAAGAGGAGCCAGAGATGCCTGTGGGGCCACGCCCCCGGCCCCTGTCTGAGCTGCACCTTAAGGAA AAGGCAGTTCCCATGCCGGAAGCCAGTGCATTTTTCATCTTCAGCCCAAACAACAGGTTCCGCCTGCAGT GCCACCGTATTGTCAATGACACGATCTTCACCAACCTCATCCTCTTCTTCATTCTGCTCAGCAGCATCTC TCTGGCTGCTGAGGACCCCGTCCAGCACACCTCCTTCAGGAACCATATGACTGCTTACGGGGCTTTCCTG CACAAGGGCTCTTTCTGCCGAAACTACTTCAATATCCTGGACCTGCTGGTGGTTAGCGTGTCCCTCATCT CCTTTGGCATCCAGTCCAGCGCGATCAACGTTGTGAAGATTTTACGAGTGCTGCGAGTCCTCAGACCCCT GAGGGCCATCAACAGGGCCAAGGGGCTAAAGCATGTGGTTCAGTGCGTGTTTGTGGCCATCCGGACCATC GGGAACATCGTAATTGTCACCACTCTGCTGCAGTTCATGTTCGCCTGCATTGGGGTCCAGCTCTTCAAGG GAAAGCTCTATACCTGTTCGGATAGTTCTAAACAGACGGAGGCAGAATGCAAGGGTAACTATATCACATA CAAAGATGGAGAGGTCGATCACCCCATTATCCAGCCTCGAAGCTGGGAGAACAGCAAGTTTGACTTTGAC AATGTTTTGGCAGCCATGATGGCTCTCTTCACCGTCTCCACCTTCGAAGGGTGGCCAGAGCTGCTGTACC GCTCCATTGACTCCCACACAGAAGACAAGGGCCCCATCTACAACTACCGTGTGGAGATCTCCATCTTCTT CATCATCTATATCATCATCATTGCCTTCTTCATGATGAACATCTTCGTGGGTTTCGTCATTGTCACCTTC CAGGAGCAGGGGGAACAAGAGTACAAGAACTGTGAGCTGGACAAGAACCAGAGACAATGTGTGGAATATG CCCTCAAGGCCCGACCCTTGCGAAGGTACATCCCCAAGAACCAGCACCAGTACAAAGTGTGGTACGTGGT CAACTCTACCTACTTCGAGTATCTGATGTTCGTTCTCATCCTGCTCAACACCATCTGCCTGGCCATGCAG CACTATGGCCAGAGCTGCCTCTTCAAAATCGCCATGAATATACTCAACATGCTTTTCACCGGCCTCTTCA CAGTGGAGATGATCCTGAAGCTCATTGCCTTCAAACCCAAGGGTTACTTTAGTGATCCCTGGAATGTTTT TGACTTCCTCATCGTCATTGGGAGCATAATTGATGTCATTCTCAGTGAGACTAATAGTGCAGAGGAGAAC TCCCGCATCTCCATCACCTTCTTCCGCCTCTTCCGGGTCATGCGCCTGGTGAAGCTGCTGAGCCGCGGGG AAGGCATCCGAACCCTGCTGTGGACCTTCATCAAGTCCTTCCAGGCTCTGCCCTATGTGGCTCTTTTGAT TGTGATGCTGTTCTTTATCTATGCAGTGATTGGGATGCAGGTGTTTGGGAAGATTGCCCTGAATGACACC ACAGAGATCAATCGGAACAACAACTTCCAGACGTTCCCCCAGGCTGTGTTACTGCTGTTCAGGTGTGCCA CCGGAGAGGCCTGGCAGGACATCATGCTGGCCTGCATGCCAGGCAAGAAGTGTGCCCCAGAGTCTGAGCC CAGCAACAGCACGGAAGGGGAGACCCCCTGTGGCAGCAGCTTTGCTGTCTTCTACTTCATCAGCTTCTAC ATGCTCTGTGCCTTCCTGATCATCAACCTCTTTGTAGCTGTTATCATGGACAACTTTGACTACCTGACTA GGGATTGGTCTATCCTCGGTCCCCATCACCTGGATGAATTCAAGAGAATCTGGGCCGAGTATGACCCTGA AGCCAAGGGTCGGATCAAACACTTGGATGTGGTGACCCTCCTCCGTCGAATTCAGCCCCCACTGGGTTTT GGGAAATTGTGTCCTCACCGTGTGGCCTGCAAACGCCTGGTGTCCATGAACATGCCTCTGAACAGCGATG GCACAGTCATGTTCAATGCTACCCTGTTTGCCCTCGTCAGGACAGCCCTGAGGATCAAAACAGAAGGGAA CCTAGAGCAAGCCAATGAGGAGCTTCGGGCCATCATCAAGAAAATCTGGAAGAGGACTAGCATGAAGCTG TTGGACCAGGTGGTGCCCCCTGCAGGCGATGACGAGGTCACAGTGGGCAAGTTCTATGCCACCTTCCTGA TCCAAGAGTACTTCAGGAAATTCAAGAAGCGAAAAGAGCAGGGGCTGGTGGGCAAGCCCTCACAAAGGAA TGCACTGTCCCTCCAGGCTGGCTTGCGCACCTTGCATGACATTGGGCCTGAGATCCGGCGGGCCATCTCT GGGGATCTGACTGCTGAGGAGGAGTTGGACAAGGCTATGAAGGAGGCGGTGTCTGCTGCCTCCGAAGATG ACATCTTCAGGAGGGCTGGAGGCCTGTTCGGCAACCATGTCACCTACTATCAGAGTGACAGCAGGGGCAA CTTTCCTCAGACGTTCGCCACCCAGCGCCCACTGCACATCAACAAGACAGGGAACAACCAAGCTGACACT GAGTCACCGTCCCATGAGAAGCTGGTGGACTCCACGTTCACCCCCAGCAGCTACTCATCCACGGGCTCCA ATGCCAACATCAACAATGCCAACAACACTGCCCTGGGCCGCTTCCCCCATCCCGCTGGCTACTCCAGCAC GGTCAGCACTGTGGAGGGCCATGGGCCTCCCTTGTCCCCTGCTGTCCGAGTACAGGAGGCAGCATGGAAG CTCAGCTCTAAGAGGTGCCACTCCCGAGAGAGCCAGGGAGCCACGGTGAATCAGGAGATATTTCCAGATG AGACCCGCAGCGTAAGGATGAGTGAAGAAGCCGAGTACTGCAGTGAGCCCAGCCTGCTCTCCACAGATAT GTTCTCCTACCAGGAAGATGAACACCGACAACTGACCTGCCCAGAGGAGGACAAGAGGGAGATCCAGCCA TCTCCAAAGAGGAGTTTCCTTCGCTCTGCCTCTCTAGGTCGAAGGGCCTCCTTCCATCTGGAATGTCTAA AGCGACAAAAGGATCAAGGGGGAGACATCTCTCAGAAGACAGCCTTGCCCTTGCATCTGGTTCATCATCA GGCATTGGCAGTGGCAGGCTTGAGCCCCCTCCTGCAGAGAAGCCATTCTCCTACCACATTCCCCAGGCCG TGCCCCACACCCCCTGTCACTCCAGGCAGCCGGGGCAGACCCCTACGGCCCATCCCTACCCTACGGCTGG AGGGGGCAGAGTCCAGCGAGAAACTCAACAGCAGCTTCCCATCCATCCACTGCAGCTCCTGGTCTGAGGA GACGACAGCCTGTAGTGGGAGCAGCAGCATGGCCCGGAGAGCCCGGCCCGTCTCCCTCACCGTGCCCAGC CAGGCTGGAGCTCCAGGGAGACAGTTCCATGGCAGTGCCAGCAGCCTGGTGGAAGCGGTCTTGATTTCAG AAGGACTGGGACAGTTTGCTCAAGATCCCAAGTTCATCGAGGTCACCACCCAGGAGCTGGCTGACGCCTG CGACATGACAATAGAGGAGATGGAGAACGCCGCAGACAACATCCTCAGTGGGGGCGCCCAGCAGAGCCCC AACGGCACCCTCTTACCTTTTGTGAACTGCAGGGACCCGGGGCAGGACAGGGCTGTGGCCCCAGAGGACG AGAGCTGCGCATATGCCCTGGGGCGAGGCCGGAGCGAGGAGGCGCTCGCGGACAGCAGGTCCTACGTCAG CAACCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231888 representing NM_001290335 Red=Cloning site Green=Tags(s) MVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIIDVPAEEDPSPCALETGHGRQCQNGTVCKP GWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAVGRDWPWIYFVTLIIIGSFFVLNLVLGVL SGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGMDEDKPRNRGAPAGLHDQK KGKFAWFSHSTETHVSMPTSETESVNTENVAGGDIEGENCGARLAHRISKSKFSRYWRRWNRFCRRKCRA AVKSNVFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFN RFDCFIVCGGILETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLF LFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLV CIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVMEKPAVEES KEEKIELKSITADGESPPTTKINMDDLQPSENEDKSPHSNPDTAGEEDEEEPEMPVGPRPRPLSELHLKE KAVPMPEASAFFIFSPNNRFRLQCHRIVNDTIFTNLILFFILLSSISLAAEDPVQHTSFRNHMTAYGAFL HKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTI GNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFD NVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTF QEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQYKVWYVVNSTYFEYLMFVLILLNTICLAMQ HYGQSCLFKIAMNILNMLFTGLFTVEMILKLIAFKPKGYFSDPWNVFDFLIVIGSIIDVILSETNSAEEN SRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDT TEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPSNSTEGETPCGSSFAVFYFISFY MLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGF GKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIKTEGNLEQANEELRAIIKKIWKRTSMKL LDQVVPPAGDDEVTVGKFYATFLIQEYFRKFKKRKEQGLVGKPSQRNALSLQAGLRTLHDIGPEIRRAIS GDLTAEEELDKAMKEAVSAASEDDIFRRAGGLFGNHVTYYQSDSRGNFPQTFATQRPLHINKTGNNQADT ESPSHEKLVDSTFTPSSYSSTGSNANINNANNTALGRFPHPAGYSSTVSTVEGHGPPLSPAVRVQEAAWK LSSKRCHSRESQGATVNQEIFPDETRSVRMSEEAEYCSEPSLLSTDMFSYQEDEHRQLTCPEEDKREIQP SPKRSFLRSASLGRRASFHLECLKRQKDQGGDISQKTALPLHLVHHQALAVAGLSPLLQRSHSPTTFPRP CPTPPVTPGSRGRPLRPIPTLRLEGAESSEKLNSSFPSIHCSSWSEETTACSGSSSMARRARPVSLTVPS QAGAPGRQFHGSASSLVEAVLISEGLGQFAQDPKFIEVTTQELADACDMTIEEMENAADNILSGGAQQSP NGTLLPFVNCRDPGQDRAVAPEDESCAYALGRGRSEEALADSRSYVSNL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001290335
ORF Size	5607 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001290335.1, NP_001277264.1
RefSeq Size	12656 bp
RefSeq ORF	5610 bp
Locus ID	12288
MW	211.3 kDa
Gene Summary	Pore-forming, alpha-1C subunit of the voltage-gated calcium channel that gives rise to L-type calcium currents (PubMed:14609949, PubMed:18586882, PubMed:21216955, PubMed:25368181, PubMed:28119464). Mediates influx of calcium ions into the cytoplasm, and thereby triggers calcium release from the sarcoplasm (By similarity). Plays an important role in excitation-contraction coupling in the heart. Required for normal heart development and normal regulation of heart rhythm (PubMed:21216955). Required for normal contraction of smooth muscle cells in blood vessels and in the intestine. Essential for normal blood pressure regulation via its role in the contraction of arterial smooth muscle cells (PubMed:14609949, PubMed:28119464). Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group (Probable).[UniProtKB/Swiss-Prot Function]

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