Plcb3 (NM_001290349) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	mKIAA4098
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229495 representing NM_001290349 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGGCGCGAGGCCCGGCGTCCACGCGCTGCAGCTGGAGCCGCCCACCGTGGTGGAGACCCTGCGGC GCGGGAGTAAGTTCATCAAATGGGACGAGGAGGCCTCCAGTCGGAACCTGGTGACCCTGCGTGTGGACCC TAATGGCTTCTTCTTGTACTGGACAGGACCCAACATGGAGGTGGACACACTGGACATCAGCTCCATCAGG GACACAAGGACAGGTCGTTATGCCCGCCTGCCCAAGGACCCTAAGATTCGAGAAGTACTGGGCTTTGGAG GTCCTGACACCCGGCTGGAGGAGAAACTGATGACAGTGGTGGCCGGGCCAGATCCAGTAAATACCACATT CTTGAACTTCATGGCCGTGCAAGATGACACAGTCAAGGTCTGGTCAGAGGAGTTGTTTAAACTGGCCATG AACATATTGGCTCAGAACGCCTCCCGGAACACCTTCCTGCGGAAAGCATACACGAAGCTGAAGCTGCAGG TGAACCAGGATGGACGGATTCCGGTCAAGAACATTCTGAAGATGTTCTCGGCGGACAAGAAGCGGGTGGA GACGGCGCTGGAGTCCTGTGGCCTCAACTTCAACCGAAGTGAGTCCATTCGCCCTGATGAGTTTCCCTTG GAAATTTTTGAGCGGTTCTTGAATAAACTGTGTCTGCGCCCGGATATTGACAAGATCCTGCTGGAGATAG GTGCCAAGGGCAAGCCGTACCTCACTCTGGAGCAGCTCATGGACTTTATCAACCAGAAGCAGAGAGACCC GAGACTCAACGAAGTGCTGTACCCGCCACTTCGGTCCTCACAGGCTCGGCTGCTCATTGAGAAGTACGAG ACGAACAAACAGTTCCTGGAGCGCGACCAGATGTCTATGGAGGGCTTCAGCCGCTACCTGGGAGGGGAGG AGAATGGTATCCTGCCTCTGGAGGCCCTGGATCTGAGCATGGACATGACCCAGCCACTGAGCGCATATTT TATCAACTCCTCACACAACACCTATCTCACTGCGGGCCAGCTGGCTGGACCATCATCGGTGGAGATGTAC CGCCAGGCACTGCTGTGGGGCTGCCGCTGTGTGGAGCTGGATGTATGGAAGGGACGGCCACCGGAGGAAG AGCCTTTCATCACTCATGGTTTCACCATGACCACTGAGGTGCCATTGCGTGATGTGCTAGAGGCCATCGC TGAGGCAGCCTTCAAGACCTCGCCCTACCCTGTCATCCTCTCCTTTGAGAACCATGTCGACTCGGCAAAG CAGCAGGCCAAGATGGCTGAGTACTGCCGCTCTATCTTTGGGGATGCGCTGCTCATTGACCCTCTCGACA AATACCCGCTATCTGCGGGCATCCCCCTGCCTAGTCCACAGGACCTGATGGGCCGTATCCTGGTGAAGAA TAAGAAGCGACATCGGCCCAGCACAGGTGTCCCTGACAGCTCAGTGCGCAAGCGGCCTCTGGAACAGAGC AACTCGGCCTTGAGTGAGAGCTCGGCCGCCACAGAGCCCTCCTCACCTCAGCTTGGGTCCCCCAGCTCCG ACAGCTGCCCTGGCCTAAGCAATGGGGAGGAGGTGGGACTCGAGAAGACCAGCCTGGAGCCTCAGAAGTC TCTAGGTGAGGAGAGCCTCAGCCGGGAACCTAATGTGCCCATGCCTGACCGTGACCGAGAGGATGAGGAG GAAGATGAAGAAGAAGAGGAAACAACGGATCCAAAAAAGCCCACCACTGATGAGGGCACAGCCAGCAGTG AGGTCAATGCCACGGAGGAGATGTCAACGCTCGTCAACTACGTTGAGCCCGTCAAGTTCAAGTCCTTCGA GGCTGCTCGAAAAAGGAACAAGTGCTTCGAGATGTCATCCTTCGTGGAGACCAAGGCAATGGAGCAACTG ACCAAGAGTCCTATGGAGTTTGTGGAATACAACAAACAGCAGCTCAGCCGCATCTACCCTAAGGGCACAC GAGTGGACTCCTCCAACTACATGCCTCAGCTCTTCTGGAACGTGGGTTGCCAGCTTGTTGCCCTCAACTT CCAGACCTTGGATTTGCCAATGCAGCTCAACGCAGGCGTGTTTGAGTACAATGGGCGCAGCGGGTACTTG CTCAAGCCCGAGTTTATGCGGCGGCCGGACAAGTCTTTTGATCCCTTCACTGAGGTCATCGTAGATGGCA TAGTGGCCAACGCCTTGCGGGTCAAGGTGATTTCGGGGCAGTTCCTGTCTGACAAGAAGGTGGGCATCTA CGTCGAGGTGGACATGTTTGGCCTCCCAGTTGACACAAGACGCAAATATCGTACCCGGACATCCCAGGGG AACTCATTCAACCCTGTGTGGGACGAGGAACCCTTTGACTTCCCCAAGGTTGTGCTGCCCACGCTGGCCT CACTTCGCATCGCAGCCTTTGAGGAGGGTGGCAAATTTGTTGGGCACCGTATCCTGCCTGTCTCTGCTAT CCGCTCAGGATACCACTATGTCTGCCTGCGAAACGAGGCCAACCAACCTTTGTGCCTGCCTGCCCTGCTT ATCTACACTGAAGCTTCTGACTACATCCCAGATGACCACCAGGACTATGCGGAGGCCTTGATTAACCCCA TCAAGCACGTAAGCCTAATGGACCAGCGGGCCAAGCAACTAGCCGCTCTCATTGGGGAGAGTGAGGCTCA GGCCAGCACAGAGACATATCAGGAGACCCCGTGTCAACAGCCAGGGTCACAGCTCCCCTCCAACCCCACA CCTAACCCACTGGATGCCTCACCTCGCTGGCCCCCCGGTCCTACCACTTCCTCCACTAGCTCCTCCCTCA GTAGCCCAGGGCAGCGAGATGATTTGATTGCCAGCATCCTCTCTGAGGTGACCCCTACACCTCTAGAGGA GCTCAGAAGCCACAAGGCTATGGTGAAGCTACGGAGCCGGCAGGATCGAGACCTGCGGGAGTTGCATAAG AAGCACCAGCGCAAGGCTGTGGCCCTCACCCGCCGCCTTCTGGATGGCCTGGCCCAGGCCAGGGCTGAGG GGAAGTGCCGGCCCTCCCCCAGTGCCCTGGGCAAGGCCACCAACTCGGAGGACGTGAAGGAGGAAGAGGA GGCCAAACAGTATCGAGAGTTCCAGAACCGACAAGTACAGAGCCTGTTGGAGCTGAGGGAAGCCCAGGCA GATGTGGAGACCAAGCGGAAGCTGGAGCATCTACGACAGGCTCATCAGCGGCTCAAGGAGGTTGTCCTGG ATACACACACAACACAGTTCAAGAGGCTGAAGGAGCTGAATGAAAGGGAGAAGAAGGAACTCCAGAAGAT CCTGGACAGGAAGCGCAACAACAGCATCTCAGAGGCCAAGACAAGGGAGAAACACAAGAAGGAGGTGGAA CTGACAGAGATTAATCGGCGGCACATCACTGAGTCGGTAAACTCCATCAGACGGCTGGAAGAGGCCCAGA AGCAGCGCCATGAACGCCTGGTGGCAGGGCAACAGCAAGTCCTCCAGCAGCTAGAGGAAGAGGAACCCAA GCTGCTGGCCCAGCTGACCCAGGAGTGTCAGGAACAGCGAGAGAGGTTGCCCCAGGAGATCCGTCGGTGC CTGCTGGGCGAGACAGCAGAGGGACTGGGGGACGGCCCCCTGGTGGCCTGTGCCAGCAATGGTCATGCAC CTGGGAGTGGTGGGCACCTGTCCAGCGCTGACTCGGAGAGCCAGGAGGAGAACACCCAGCTTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001290349
Insert Size	3705 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001290349.1, NP_001277278.1
RefSeq Size	4273 bp
RefSeq ORF	3705 bp
Locus ID	18797
UniProt ID	P51432
Gene Summary	The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) encodes isoform a.

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