Plcb3 (NM_001290349) Mouse Tagged ORF Clone
CAT#: MR231706
- TrueORF®
Plcb3 (myc-DDK-tagged) - Mouse phospholipase C, beta 3 (Plcb3), transcript variant 1
"NM_001290349" in other vectors (1)
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CNY 9,600.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | mKIAA4098 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR231706 representing NM_001290349
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGGCGCGAGGCCCGGCGTCCACGCGCTGCAGCTGGAGCCGCCCACCGTGGTGGAGACCCTGCGGC GCGGGAGTAAGTTCATCAAATGGGACGAGGAGGCCTCCAGTCGGAACCTGGTGACCCTGCGTGTGGACCC TAATGGCTTCTTCTTGTACTGGACAGGACCCAACATGGAGGTGGACACACTGGACATCAGCTCCATCAGG GACACAAGGACAGGTCGTTATGCCCGCCTGCCCAAGGACCCTAAGATTCGAGAAGTACTGGGCTTTGGAG GTCCTGACACCCGGCTGGAGGAGAAACTGATGACAGTGGTGGCCGGGCCAGATCCAGTAAATACCACATT CTTGAACTTCATGGCCGTGCAAGATGACACAGTCAAGGTCTGGTCAGAGGAGTTGTTTAAACTGGCCATG AACATATTGGCTCAGAACGCCTCCCGGAACACCTTCCTGCGGAAAGCATACACGAAGCTGAAGCTGCAGG TGAACCAGGATGGACGGATTCCGGTCAAGAACATTCTGAAGATGTTCTCGGCGGACAAGAAGCGGGTGGA GACGGCGCTGGAGTCCTGTGGCCTCAACTTCAACCGAAGTGAGTCCATTCGCCCTGATGAGTTTCCCTTG GAAATTTTTGAGCGGTTCTTGAATAAACTGTGTCTGCGCCCGGATATTGACAAGATCCTGCTGGAGATAG GTGCCAAGGGCAAGCCGTACCTCACTCTGGAGCAGCTCATGGACTTTATCAACCAGAAGCAGAGAGACCC GAGACTCAACGAAGTGCTGTACCCGCCACTTCGGTCCTCACAGGCTCGGCTGCTCATTGAGAAGTACGAG ACGAACAAACAGTTCCTGGAGCGCGACCAGATGTCTATGGAGGGCTTCAGCCGCTACCTGGGAGGGGAGG AGAATGGTATCCTGCCTCTGGAGGCCCTGGATCTGAGCATGGACATGACCCAGCCACTGAGCGCATATTT TATCAACTCCTCACACAACACCTATCTCACTGCGGGCCAGCTGGCTGGACCATCATCGGTGGAGATGTAC CGCCAGGCACTGCTGTGGGGCTGCCGCTGTGTGGAGCTGGATGTATGGAAGGGACGGCCACCGGAGGAAG AGCCTTTCATCACTCATGGTTTCACCATGACCACTGAGGTGCCATTGCGTGATGTGCTAGAGGCCATCGC TGAGGCAGCCTTCAAGACCTCGCCCTACCCTGTCATCCTCTCCTTTGAGAACCATGTCGACTCGGCAAAG CAGCAGGCCAAGATGGCTGAGTACTGCCGCTCTATCTTTGGGGATGCGCTGCTCATTGACCCTCTCGACA AATACCCGCTATCTGCGGGCATCCCCCTGCCTAGTCCACAGGACCTGATGGGCCGTATCCTGGTGAAGAA TAAGAAGCGACATCGGCCCAGCACAGGTGTCCCTGACAGCTCAGTGCGCAAGCGGCCTCTGGAACAGAGC AACTCGGCCTTGAGTGAGAGCTCGGCCGCCACAGAGCCCTCCTCACCTCAGCTTGGGTCCCCCAGCTCCG ACAGCTGCCCTGGCCTAAGCAATGGGGAGGAGGTGGGACTCGAGAAGACCAGCCTGGAGCCTCAGAAGTC TCTAGGTGAGGAGAGCCTCAGCCGGGAACCTAATGTGCCCATGCCTGACCGTGACCGAGAGGATGAGGAG GAAGATGAAGAAGAAGAGGAAACAACGGATCCAAAAAAGCCCACCACTGATGAGGGCACAGCCAGCAGTG AGGTCAATGCCACGGAGGAGATGTCAACGCTCGTCAACTACGTTGAGCCCGTCAAGTTCAAGTCCTTCGA GGCTGCTCGAAAAAGGAACAAGTGCTTCGAGATGTCATCCTTCGTGGAGACCAAGGCAATGGAGCAACTG ACCAAGAGTCCTATGGAGTTTGTGGAATACAACAAACAGCAGCTCAGCCGCATCTACCCTAAGGGCACAC GAGTGGACTCCTCCAACTACATGCCTCAGCTCTTCTGGAACGTGGGTTGCCAGCTTGTTGCCCTCAACTT CCAGACCTTGGATTTGCCAATGCAGCTCAACGCAGGCGTGTTTGAGTACAATGGGCGCAGCGGGTACTTG CTCAAGCCCGAGTTTATGCGGCGGCCGGACAAGTCTTTTGATCCCTTCACTGAGGTCATCGTAGATGGCA TAGTGGCCAACGCCTTGCGGGTCAAGGTGATTTCGGGGCAGTTCCTGTCTGACAAGAAGGTGGGCATCTA CGTCGAGGTGGACATGTTTGGCCTCCCAGTTGACACAAGACGCAAATATCGTACCCGGACATCCCAGGGG AACTCATTCAACCCTGTGTGGGACGAGGAACCCTTTGACTTCCCCAAGGTTGTGCTGCCCACGCTGGCCT CACTTCGCATCGCAGCCTTTGAGGAGGGTGGCAAATTTGTTGGGCACCGTATCCTGCCTGTCTCTGCTAT CCGCTCAGGATACCACTATGTCTGCCTGCGAAACGAGGCCAACCAACCTTTGTGCCTGCCTGCCCTGCTT ATCTACACTGAAGCTTCTGACTACATCCCAGATGACCACCAGGACTATGCGGAGGCCTTGATTAACCCCA TCAAGCACGTAAGCCTAATGGACCAGCGGGCCAAGCAACTAGCCGCTCTCATTGGGGAGAGTGAGGCTCA GGCCAGCACAGAGACATATCAGGAGACCCCGTGTCAACAGCCAGGGTCACAGCTCCCCTCCAACCCCACA CCTAACCCACTGGATGCCTCACCTCGCTGGCCCCCCGGTCCTACCACTTCCTCCACTAGCTCCTCCCTCA GTAGCCCAGGGCAGCGAGATGATTTGATTGCCAGCATCCTCTCTGAGGTGACCCCTACACCTCTAGAGGA GCTCAGAAGCCACAAGGCTATGGTGAAGCTACGGAGCCGGCAGGATCGAGACCTGCGGGAGTTGCATAAG AAGCACCAGCGCAAGGCTGTGGCCCTCACCCGCCGCCTTCTGGATGGCCTGGCCCAGGCCAGGGCTGAGG GGAAGTGCCGGCCCTCCCCCAGTGCCCTGGGCAAGGCCACCAACTCGGAGGACGTGAAGGAGGAAGAGGA GGCCAAACAGTATCGAGAGTTCCAGAACCGACAAGTACAGAGCCTGTTGGAGCTGAGGGAAGCCCAGGCA GATGTGGAGACCAAGCGGAAGCTGGAGCATCTACGACAGGCTCATCAGCGGCTCAAGGAGGTTGTCCTGG ATACACACACAACACAGTTCAAGAGGCTGAAGGAGCTGAATGAAAGGGAGAAGAAGGAACTCCAGAAGAT CCTGGACAGGAAGCGCAACAACAGCATCTCAGAGGCCAAGACAAGGGAGAAACACAAGAAGGAGGTGGAA CTGACAGAGATTAATCGGCGGCACATCACTGAGTCGGTAAACTCCATCAGACGGCTGGAAGAGGCCCAGA AGCAGCGCCATGAACGCCTGGTGGCAGGGCAACAGCAAGTCCTCCAGCAGCTAGAGGAAGAGGAACCCAA GCTGCTGGCCCAGCTGACCCAGGAGTGTCAGGAACAGCGAGAGAGGTTGCCCCAGGAGATCCGTCGGTGC CTGCTGGGCGAGACAGCAGAGGGACTGGGGGACGGCCCCCTGGTGGCCTGTGCCAGCAATGGTCATGCAC CTGGGAGTGGTGGGCACCTGTCCAGCGCTGACTCGGAGAGCCAGGAGGAGAACACCCAGCTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR231706 representing NM_001290349
Red=Cloning site Green=Tags(s) MAGARPGVHALQLEPPTVVETLRRGSKFIKWDEEASSRNLVTLRVDPNGFFLYWTGPNMEVDTLDISSIR DTRTGRYARLPKDPKIREVLGFGGPDTRLEEKLMTVVAGPDPVNTTFLNFMAVQDDTVKVWSEELFKLAM NILAQNASRNTFLRKAYTKLKLQVNQDGRIPVKNILKMFSADKKRVETALESCGLNFNRSESIRPDEFPL EIFERFLNKLCLRPDIDKILLEIGAKGKPYLTLEQLMDFINQKQRDPRLNEVLYPPLRSSQARLLIEKYE TNKQFLERDQMSMEGFSRYLGGEENGILPLEALDLSMDMTQPLSAYFINSSHNTYLTAGQLAGPSSVEMY RQALLWGCRCVELDVWKGRPPEEEPFITHGFTMTTEVPLRDVLEAIAEAAFKTSPYPVILSFENHVDSAK QQAKMAEYCRSIFGDALLIDPLDKYPLSAGIPLPSPQDLMGRILVKNKKRHRPSTGVPDSSVRKRPLEQS NSALSESSAATEPSSPQLGSPSSDSCPGLSNGEEVGLEKTSLEPQKSLGEESLSREPNVPMPDRDREDEE EDEEEEETTDPKKPTTDEGTASSEVNATEEMSTLVNYVEPVKFKSFEAARKRNKCFEMSSFVETKAMEQL TKSPMEFVEYNKQQLSRIYPKGTRVDSSNYMPQLFWNVGCQLVALNFQTLDLPMQLNAGVFEYNGRSGYL LKPEFMRRPDKSFDPFTEVIVDGIVANALRVKVISGQFLSDKKVGIYVEVDMFGLPVDTRRKYRTRTSQG NSFNPVWDEEPFDFPKVVLPTLASLRIAAFEEGGKFVGHRILPVSAIRSGYHYVCLRNEANQPLCLPALL IYTEASDYIPDDHQDYAEALINPIKHVSLMDQRAKQLAALIGESEAQASTETYQETPCQQPGSQLPSNPT PNPLDASPRWPPGPTTSSTSSSLSSPGQRDDLIASILSEVTPTPLEELRSHKAMVKLRSRQDRDLRELHK KHQRKAVALTRRLLDGLAQARAEGKCRPSPSALGKATNSEDVKEEEEAKQYREFQNRQVQSLLELREAQA DVETKRKLEHLRQAHQRLKEVVLDTHTTQFKRLKELNEREKKELQKILDRKRNNSISEAKTREKHKKEVE LTEINRRHITESVNSIRRLEEAQKQRHERLVAGQQQVLQQLEEEEPKLLAQLTQECQEQRERLPQEIRRC LLGETAEGLGDGPLVACASNGHAPGSGGHLSSADSESQEENTQL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001290349 |
ORF Size | 3702 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001290349.1, NP_001277278.1 |
RefSeq Size | 4273 bp |
RefSeq ORF | 3705 bp |
Locus ID | 18797 |
UniProt ID | P51432 |
MW | 139.9 kDa |
Gene Summary | The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC229495 | Plcb3 (untagged) - Mouse phospholipase C, beta 3 (Plcb3), transcript variant 1 |
CNY 11,880.00 |